The relationship between miR34c, miR15b, the quality of sperm parameters and sperm DNA damage rate in infertile oligoasthenoteratozoospermia men

MiRNAs play an important role in various pathophysiological processes in cells and controlling the process of apoptosis in sperm. The aim of this study is to investigate the relationship between the expression of miR34c and miR15b, the quality of sperm parameters and the rate of sperm DNA breakage in oligoasthenoteratozoospermia infertile individuals.

This study was conducted on 25 patients with oligoasthenoteratozoospermia who had referred to Jihad University Infertility Treatment Center in Qom. 25 fertile people have been selected as the control group. After collecting sperm samples, sperm parameters were analyzed according to the World Health Organization (WHO 2010). The amount of sperm DNA breakage was checked using the SCD technique. The expression levels of miR34c and miR15b were measured using the Rael-time PCR technique.

According to the obtained results, sperm parameters such as concentration, motility and morphology of sperm in infertile oligoasthenoteratozoospermia individuals show a significant decrease compared to fertile individuals (P<0.05) and the rate of sperm DNA breakage in oligoasthenoteratozoospermia individuals increases. It was significant (P<0.05). Correlation test revealed that there is a significant correlation between sperm variables including concentration, motility, normal morphology, sperm DNA breakage rate and the expression level of miR34c and miR15b.

Our findings showed that there is a significant relationship between the expression of miR34c, miR15b, sperm parameters and sperm DNA health. In fact, these results can provide new insights for the diagnosis of male infertility at the molecular level.