Investigating the Effect of an Aerobic Exercise Course on Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase in Heart Tissue of Male Rats with Diabetes

Inactivity plays a role in diabetes and increasing tissue oxidative stress and this study aimed to investigate the effect of a period of aerobic exercise on malondialdehyde, superoxide dismutase, and glutathione peroxidase in the heart tissue of male diabetic rats.
44 male Wistar rats (200-250 grams) were randomly divided into 4 ten groups of healthy, diabetic, training, and diabetes+ training. After 12 hours of food deprivation, diabetes was induced by intraperitoneal injection of STZ solution, The training groups did aerobic exercise 5 days a week for 6 weeks. 48 hours after completion of the protocol, anesthetized animals and heart tissue were removed. ELISA method was used to measure the amount of superoxide dismutase, malondialdehyde, and glutathione peroxide. Data were analyzed with one-way ANOVA, Tukey's post hoc tests, SPSS, and GraphPad software.
The results showed that superoxide dismutase and glutathione peroxide in the diabetes group were significantly lower than the control, exercise, and diabetes+exercise groups (p≤0.0001). Also, superoxide dismutase and glutathione peroxide were significantly higher in the diabetes+exercise group than in the control and exercise groups (p≤0.0001). Malondialdehyde in the diabetes group was significantly higher than in the diabetes+exercise group, the exercise group, and the control group (p≤0.0001). Malondialdehyde was significantly higher in the diabetes+exercise group than in the diabetes and control groups (p≤0.0001).
aerobic training can have a positive effect on the heart tissue in diabetic rats by reducing oxidative stress.