Comparison of GuttaFlow & Gutta-percha cytotoxicity on rat's L929 cells

A new root filling material, GuttaFlow, has been introduced by adding gutta-percha to RoekoSeal playing the role of gutta-percha and sealer simultaneously. The present study evaluated the cytotoxicity of two root filling materials GuttaFlow and gutta-percha on mouse fibroblast cell line L929. GuttaFlow and gutta-percha were prepared according to manufacturer’s instruction and exposed to UV radiation under the hood. After passage of mouse L929 fibroblast cells for 4 times, their vitality was assessed by tripan blue and counted by neobar devices. The counted cells were placed in the 24-well plates. The cell and dimethyl methacrylate were used as negative and positive controls. The fibroblasts were cultured at 37° C in a humidified atmosphere of %95 air and %5 Co2 in Dulbecco’s MEM supplemented with %10 FBS containing penicillin (10 IU/ ml) and streptomycin (10 µg/ml solution. 10 specimens of each experimental and control groups were brought into contact with the culture medium and incubated after 24 hours under sterilized condition. The cytotoxicity of each samples were assessed by MTT test after 1 hour, 24 and 72 hours. The results were analyzed using one-way and two-way ANOVA and Tukey multiple comparison interval. There were a significant effect of material, assessment time and their interaction on optical density of material’s cytotoxicity (p<0.0001 in all conditions), also it was revealed that assessment time had a significant influence on optical density of both materials. In GuttaFlow, the most and least cytotoxicity was observed at 24 and 72 hours while cytotoxicity of Gutta-percha increased with time