Optimizing Regeneration of Cotton Plant via Shoot Apex

Optimizing regeneration of cotton plant in two variety (Sahel and Varamin) via shoot apex was done in order to Agrobacterium-mediated transformation. In this reaserch shoot apexes of two varieties were isolated from four or five day seedling and were placed on a special medium of shooting (modified MS without hormon).In order to select the best rooting media, The Statistical Analysis explants that produce shoot and leaves in a CRBD with 4 replicates and 4 rooting treatments: 1) modified MS without hormon, 2) ½ MS with 0.1 mg/lit IBA, 3) ½ MS with 0.1 mg/lit NAA, 4) ½ MS with 0.1 mg/lit IAA. The statistical analysis indicated that the best for both varieties, was medium with 0.1 mg/lit IBA and rooting percentage of Varamin is better than sahel in all of media.