Cross reaction of human antibody to Hydatid Cyst Fluid antigens of human and mice

The aim of the current study was to assess the cross reaction of the crude hydatid cyst fluid (HCF) antigens of Echinococcus granulosus, obtained from mice experimentally infected with hydatid cyst and from naturally infected human using sera of infected human who had surgery at different hospitals in Iran. hydatid cysts obtained from lungs and livers of naturally infected sheep slauthered from local abattoirs in Ilam, Iran. HCF recovered from cysts was centrifuged at 10000 rpm for 25 minutes. The supernatant was dialysed against PBS, freezed- thawed and used as crude HCF. HCFs obtained from two different host species were highly useful for ELISA and most sera from CE patients equally recognized the HCF antigen of both mice and human. Mice sera showed a cross-reaction with human sera in recognition of HCF antigens of echinococcosis. IgG4 was the highest IgG subclass recognizing HCF of both mice and human HCF. Human IgG was high in response to both antigens while the OD ratio was two times using human IgG against mice antigen compared to human antigen. IgG and IgG4 of human sera are reacting against both mice and human HCF with the greatest response from IgG total to mice HCF. Cross reaction of human IgG and IgG4 was observed for both mice and human HCF. Mice HCF can be relied on as an appropriate antigen in detection of human hydatidosis.