Immunological Evaluation of Recombinant HIV1 Virions with Novel Adjuvants in BALB/c Mice Model

Background and Finding an efficient vaccine against HÏV infection is still a major concern and a great challenge. Ïn our previous study, HÏV-1 virions with capability of a single replication were produced. Ïn the present study their immunological evaluation has been carried out to study their induction of cellular and humoral responses in mice model. Ïn this study, first and second virion generations were produced in human HËK293T cells, and were injected to three different mice groups in three doses with and without adjuvants. Âfter the last injection, immune responses were evaluated in serum and spleen cells of mice using ËLÏSÂ. Âlthough all of mice vaccinated by different immunogen regimens did develop cellular responses, in the main group injected with novel adjuvants the highest levels of specific antibody, ÏFN-γ, ÏL-4 an also most ÏFN-γ excreting cells were evident, which is suggestive of a strong cellular response in these cells.Çonclusion: Ân effective vaccine against HÏV requires induction of strong humoral and cellular responses. Hence, these results demonstrate the high immune system stimulating capabilities of virion injection regime accompanied by M70 and ÇpG adjuvants, which provides the potential for an efficient HÏV vaccine.