Moringa oleifera is known as a rich and cost-effective source of phytochemical compounds and has many applications in industry, water treatment, pharmacy and biofuels The leaves of Moringa oleifera were introduced by the Taiwan Plant Center as one of the 120 food species studied as the plant with the highest nutritional content. In this study, the effect of different extraction methods such as Maceration and Soxhlet on phenolic content, flavonoid content and antioxidant properties, The effect of these extracts on A549 lung cancer cells was also evaluated by MTT assay. This plant was studied. According to the results obtained from the leaves of Moringa oleifera, the highest extraction efficiency was related to Soxhlet extraction. The highest phenolic and flavonoid content was related to Maceration extraction method. The results of studying the antioxidant properties of the extract showed that most of the antioxidant properties were related to the extract obtained by Soxhlet method. MTT test showed that among the various extracts, the lowest IC50 was related to the extract extracted by Soxhlet method. This study shows that Moringa oleifera leaves are a good nutritional source.

Due to the stability, water and environmental pollution, and health concerns of phenol compounds, phenol removal is important. For this purpose, the present study evaluated the efficiency of phenol removal of activated carbon derived from Moringa oleifera pod from aqueous solutions.
In this study, the Moringa oleifera pod was used as carbon source in activated carbon preparation. The activated carbon preparation protocol included dehydration, debris removal, and thermal activation. After preparation of activated carbon, the effective parameters on phenol adsorption process including solution pH, contact time, initial concentration of phenol, and activated carbon doses were studied. In addition, the obtained data was tested via Langmuir and Freundlich isotherms.
Findings: The obtained results showed that the highest phenol adsorption was achieved at neutral solution pH. With increasing or reducing of solution pH, phenol removal efficiency decreased. Moreover, with increasing of contact time from 5 minutes to 840 minutes, phenol removal efficiency improved from 23% to 94%. In addition, with increasing of initial concentration of phenol, phenol removal efficiency had a descending trend. Thus, with the use of 0.5 g/l activated carbon and initial concentration of 7.5 mg/l, phenol removal efficiency was 76%, and at the concentrations of 10 and 15 mg/l, removal efficiency reduced to 71% and 66%, respectively. The obtained results were in line with the Freundlich isotherm and maximum adsorption capacity varied from 6.5 to 7.2 mg/g in the studied initial phenol concentration.
The obtained results demonstrated that activated carbon derived from Moringa oleifera pod had high efficiency in phenol removal from aqueous solutions. With respect to the fact that the Moringa oleifera is grown in southern Iran and does not have a specific application, the Moringa oleifera pod could be considered as a low cost source for activated carbon preparation.

One of the classifications of peripheral neuropathy causes is due to the use of chemotherapeutic agents called chemotherapy-induced peripheral neuropathy (CIPN) conditions. Administration of platinum groups resulted in changes in the expression and function of Transient Receptor Potential Vanilloid type 1 (TRPV1) as well as altered neuronal excitation and propagation of nociceptive sensory signals. Moringa oleifera and Caesalpinia sappan L. are reported for their neuroprotective effect. In this study, we conducted a molecular docking study for 63 secondary metabolites of Moringa oleifera and 27 secondary metabolites of Caesalpinia sappan L. using an in silico approach targeting TRPV1 (PDB ID: 5IS0) using AutoDockVina software. ADMET characteristics were predicted using the SwissADME and pkCSM Online Tool. This study found that the binding energy of the six metabolites of Moringa oleifera (quercetin, ellagic acid, lutein, luteolin, rhamnetin, and 3-O-beta-D-Glucopyranosyl sitosterol) and three metabolites of Caesalpinia sappan L. (ombuin, phanginin I, and phanginin J) lower than native ligand through TRPV1 protein. This compounds are potential to be developed as a candidate for antagonist TRPV1. Furthermore, this study became basic data for developing TRPV1 antagonist-targeted therapy, especially in CIPN conditions.

In this study, green synthesis assisted colloidal solution system was used to prepare monodispersed spherical silver nanoparticles from hydroponic Moringa oleifera Lam. leaf extract. The Moringa oleifera leaf extract used has shown great potential in the biosynthesis of silver nanoparticles for medicinal and biotechnological applications. The formation of silver nanoparticles was observed by UV-Vis spectroscopy with a surface plasmon resonance band at 425 nm after 1 hour of preparation. Transmission electron microscopy (TEM) imaging shows that the nanoparticles are spherical in shape with an average size of 20 nm. The atomic force microscopy (AFM) image showed that the Ag nanoparticles were spherical with good monodispersity, confirming the TEM results. The biomedical use of the green synthesized silver nanoparticles from medicinal plants as antimicrobial and cytotoxic agents can be suggested by their strong bioactivity and synergistic effect of Moringa oleifera and silver nanoparticles.

Pain is a severe symptom of many diseases, with an increasing percentage of people manifesting various types of pain. Medicinal plants provide analgesic potential with little toxicity. We performed this experiment to compare the analgesic activities of Lawsonia inermis, Waltheria indica, Moringa oleifera, and Nigella sativa in Wistar rats using writhing and paw lick responses. We grouped 21 adult female rats into seven groups (n=3), including uninduced and untreated rats (group 1), induced untreated rats (group 2), rats treated with Lawsonia inermis at 200 mg/kg (group 3), rats treated with Waltheria indica at 200 mg/kg (group 4), rats treated with Nigella sativa at 200 mg/kg (group 5), rats treated with Moringa oleifera at 200 mg/kg (group 6), and rats treated with diclofenac at 10 mg/kg (group 7). We dosed rats for 14 days after inducing the pain. Phytochemical screening showed that methanolic extracts of Lawsonia inermis, Moringa oleifera, and ethanolic extract of Waltheria indica contain: Alkaloid, saponin, steroid, tannin, flavonoid, phenols, terpene, and glycosides. The rate of weight gain in rats treated with M. oleifera and W. indica was 7%, and with diclofenac was 9% compared to the untreated control. L. inermis and N. sativa possessed a weight gain of 3% and 2%, respectively. All the extracts exhibited analgesic activities by significantly reducing the number of lick and writh in the order of Lawsonia inermis, Nigella sativa, Moringa oleifera, and Waltheria indica.This study concluded that Lawsonia inermis possess significant analgesic activities compared to other plants and the standard drug (diclofenac).

Moringa oleifera Lam. belong the family Moringaceae is introduced and recorded for the first time in the flora of Libya, this record is add a new family as well because the family Moringaceae was not recorded in the flora of Libya. The plants of M. oleifera were seen in petroleum clinic garden in Tripoli in the North West part of Libyan, and Ghat hospital garden in the south-west of the country were seen few trees of Moringa oleifera in both hospitals, these trees were introduced by Indian physicians who were worked at these hospitals in the past, they introduced Moringa tree for its medicinal and nutritional value. This species was also introduced in the last four years and cultivated in many parts of the country for the purpose of nutritional and medicinal value. Morphological description is provided to facilitate further identification of this species and to warrant its future detection as well.

The current study aimed to know the effects of oral administration of Moringa oleifera leaves powder which contains the active substances to prevent the harmful toxic effect of chromium Cr (potassium dichromate; VI) on some hormonal and biochemical tests, in addition to understand the relationship between active compound of Moringa oleifera on testes and liver tissues against chromium toxicity. Thirty-three healthy male white New Zealand rabbits were randomly distributed in three groups (11 rabbits in each group). The first group were orally drenched normal saline considered as a control group. The second, were treated with chromium (40 mg kg-1 body weight/day) to notice experimental toxicity on some blood parameters and tissues structure., The third with 300 mg Moringa oleifera powder /kg diet/day with the chromium (40 mg kg-1 body weight/day). Treating for all groups was continued for 60 days. The results showed negative effects as a significant decrease (p≤ 0.01) in testosterone and inhibin-B hormone, glutathione (GSH) and total antioxidant capacity (TAC), by the increased level of FSH, alanin amino transferase (ALT), aspartate amino transferase (AST), liver cholesterol and malondialdehyde (MDA), in addition to histological variation in liver and testes in the rabbit serum dealt with chromium (group 2). In contrast, a positive effect was observed as a significant raise in testosterone, inhibin-B hormone, GSH and TAC, by decreased levels of FSH, ALT, AST, liver cholesterol and MDA in the serum dealt with Moringa oleifera (group 3), in addition to the improvement effects on testicular tissue and liver to be closed to normal concentration in control group.

Moringa oleifera is a small tree with very significant nutritional but alsopharmacological properties and various preparations from almost all parts of the plant (leaves,fruit, stems, bark, roots) are used in folk medicine for treating several ailments. This study aimedto investigating the polyphenolic burden and in vitro antioxidant properties of M. oleifera rootextracts, obtained with solvents covering a range of polarities. Lyophilised Moringa oleifera roots were extracted with solvents of increasing polarity,including ethyl acetate, butanol, methanol and water. The generated extracts were screened forpolyphenolic load and antioxidant activities, by determining the total polyphenol, total f lavonoid,total f lavanol, antiradical activity and reducing power. Liquid chromatography-mass spectrometrywas also employed to obtain some evidence regarding the nature of the main constituents. The results indicated that both aqueous and methanolic extracts were the richestin total polyphenols, but the aqueous extract also exhibited high reducing power. The liquidchromatography-diode array-mass spectrometry analysis also revealed that the major substancesoccurring in the aqueous extract were relatively polar molecules, but the mass spectral data wereambiguous to assign tentative structures. Polar solvents, such as water, may be effectively used to recover high amounts of M.oleifera root phenolics, which possess important antioxidant properties.

Phytochemical and antimicrobial analysis of Moringa oleifera seeds were carried using standard laboratory procedures. The phytochemical content showed the presence of alkaloid, flavonoid, saponin, and steroids respectively as samples contained 5.85g alkaloid, 5.97g flavonoid, and 1.46g saponin. Anti-microbial screening with certain selected human pathogens and the zones of inhibition obtained showed that alkaloids were 3mm, 3mm, 2mm, 4mm, & 5mm, flavonoids were 6mm, 5mm, 4mm, 2mm and 7mm and saponins were 4mm, 3mm, 3mm, 6mm and 5mm for Escherichia coli, Pseudomonas aureginosa, Klebsiella, Staphylococcus aureus and Streptocuccus aureus. Minimum inhibitory concentration (MIC) were 6.5mg/cm3, 12.5mg/cm3, 12.5mg/cm3, 6.5mg/cm3 and 12.5mg/cm3 for Escherichia coli, Pseudomonas aureginosa, Klebsiella, Staphylococcus aureus and Streptocuccus aureus respectively. This finding shows that the application of the seed extract of Moringa oleifera for potential therapeutic use and medicinal drugs in treatment of pneumonia, urinary tract infection and a host of other diseases in humans.

The work presents a one-step green synthesis of green luminescent C-dots using Moringa Oleifera leaves as precursor which has not been explored as a source or precursor for the fabrication of the lowest dimensional carbon material. The presence of calcium ion in the bulk structure and the surface passivated functional groups of the C-dot with a size of 1-2 nm were well correlated using extensive spectroscopic tools. The as-prepared C-dots were investigated as a fluorescent probe for detection of heavy metal ions such as Fe(III), Hg(II), Cu(II), Pb(II), Cr(VI) and As(III). The C-dots were extrapolated as a potent turn-off sensor for Cr(VI) ion in the range of 0.05-10 mM. The study provided a new approach to the application of Moringa Oleifera leaves for nanotechnology.