مجله زیست شناسی جانوری
سال چهاردهم شماره 2 (زمستان 1400)

In obese people, the expression level of interleukin is one beta higher, so that the expression level of interleukin 1 beta is directly related to the level of body mass index and fat mass. This study aimed to determine the effects of six weeks of aerobic exercise, consumption of cinnamon extract and high fat diet on the expression of 1 beta interleukin gene in liver tissue of male rats fed a high fat diet using two-month-old male Wistar rats. Fifty two-month-old male rats were divided into five control groups: high-fat diet, cinnamon extract, and high-fat diet, aerobic training and high-fat diet, and aerobic training and cinnamon extract and high-fat diet after two weeks of control. Cinnamon extract at a rate of 200 mg per kg of body weight and emulsion of high-fat food in excess of water and normal diet of rodents daily at a rate of 1.5 mg per kg of body weight for six weeks by gavage. The training group participated in an aerobic exercise program on a treadmill five days a week for six weeks. The mean of research variables among groups (except the control group) was compared with one-way variance analysis and Bonferroni tests to determine the differences among groups. The mean of research variables between each group was compared with the control group to determine the differences within the group by one-sample t-test at the significant level of p 05 0.05. There was a significant difference between body weight and liver tissue weight in the post-test between all groups. The difference among the mean variables of one beta interleukin in the liver tissue of male rats fed a high-fat diet after six weeks of aerobic exercise, consumption of cinnamon extract, and high-fat diet was significant. Therefore, it seems that the effects of high fat diet, high fat diet and aerobic exercise, high fat diet, and cinnamon and high-fat diet, and aerobic exercise and cinnamon on the interleukin variable of one beta in liver tissue are significant and significant.

Cardiovascular diseases are the leading causes of mortality and death in all areas of the world except Africa. This study aimed at the effect of eight-week combined training on Endostatin and VEGF gene expression in CABG patients.This is an applied and semi-experimental research with two groups (interventional and control), which was conducted as a pretest and posttest. 30 middle-aged CABG men were selected using purposive sampling based on inclusion criteria. Participants were randomly divided into two groups of control and combined training. Quantitative Real-time PCR was utilized  to measure Endostatin and VEGF gene expression levels. The eight-week combined exercise training was conducted in three sessions per a week. Dependent t-test and independent t-test were used to data analysis. A significant increase was observed after eight weeks of combined training on VEGF gene expression in CABG  patients. (p=0.001), but it was not Significant on Endostatin gene expression( p=0.38) . Eight weeks of combined  Cardiac Rehabilitation training as the protocol used in  this research increased the expression of the VEGF gene in CABG patients , but there were not any significant changes on Endostatin gene expression by this way. As a result, the increase in the VEGF gene is one of the results of adaptation to the combined exercise activity used in this study, and it appears that physical activity is an important and effective factor in the process of angiogenesis, and can play a key role in the prevention of cardiovascular diseases.

Glioblastoma multiforme (also called glioblastoma, GBM, or grade 4 astrocytoma) is uncommon cancer which accounts for more than half of the primary malignant brain tumors diagnosed. The study of the expression of genes involved in the glioblastoma can be effective in early detection, so we discussed the clinical significance and biological role of the SMC4 gene transcript in glioblastoma cancer, which is unknown. Before treatment, 50 samples of healthy tissue margin from a glioblastoma tumor were taken as a control group, and 50 samples of glioblastoma tissue were acquired as a patient group. We did RNA extraction and cDNA synthesis. qRT-PCR was used to determine SMC4 expression levels. As an internal control, ACTB was employed. Statistical analysis was performed by GraphPad Prism v.8.0.1. ROC curve was plotted.Overexpression of SMC4 (P<0.0001) in glioblastoma tissue samples was significant compared to healthy tumor margin tissue. While in age (P<0.7507) and sex (P<0.2270) no significant expression was observed. Increased expression of SMC4 gene can play an important role in the glioblastoma cancer. Our work provides a new perspective on SMC4 gene transcription with respect to ROC curve analysis as a biomarker, but needs further study.

Nowadays, the nanotechnology development has expanded to various medical fields, drug delivery, etc. novel drug delivery systems have made diseases cure such as cancer easier. Alumina nanoparticles have a vast use in this area due to their thermal properties. Among numerous applications uses of these nanoparticles, one of the hazards to discuss is their toxicity. Besides, surface modification of these nanoparticles with chitosan as a biopolymer lead to the bioavailability improvement. In this research, alumina NPs were covered with chitosan biopolymer. Analysis of the size of particles indicated about 70 nm. FT-IR analysis on alumina and alumina/chitosan NPs showed surface modification of alumina NPs greatly. Besides, TEM images of the nanoparticles approved suitable size for drug delivery purposes. HeLa cells were treated with Alumina and Alumina/CS for 24 h, and IC50 concentration was determined. Toxic effects of these components on the cells were evaluated by MTT assay and acridine orange/ethidium bromide (AO/EB) staining. The results of the bioassay of nanoparticles well showed their successful coating by chitosan polymer. Moreover, the results detected that Alumina samples had toxic effects on the cells in a dose dependent manner and their IC50 concentration was about 25 mM. While coated samples had significantly lower toxicity and it had no significant toxicity on HeLa cells in concentrations below 5 mM.

Streptococcosis and Yersiniosis are important diseases for the aquaculture industry for the rainbow trout (Oncorhynchus mykiss).  In this study, the specific immunological responses of rainbow trout to vaccination with the bivalent vaccine of Streptococcus/ Yersinios were investigated. Three hundred rainbow trout fingerlings (25±2g) were randomly divided into four equal groups in triplicates. Treatments contain Streptococcosis vaccine, Yersiniosis vaccine, bivalent vaccine, and control group.  Fish in vaccinated treatment intraperitoneally injected with 100 microliter vaccine on the first day. The control group was injected with sterile PBS, identical to the vaccinated group. Blood samples were collected from vaccinated and non-vaccinated groups at days 0, 30, and 60 of the study, and antibody titer was subsequently performed. Sixty days after initial immunization, all groups were challenged by a lethal dose of Streptococcus iniae and Yersinia rockeri, mortality rate was calculated and compared among the groups for 14 days. These results indicated high antibody levels in the vaccinated fish at day 30 and 60 post-immunization using both vaccines(p<0.05). However, this amount was fewer at day 60 than day 30. The percentage of trout cumulative mortality after the challenge with Streptococcus iniae and Yersinia rockeri showed a significant decrease in vaccinated groups compared to the control treatment. In general, it can be concluded that the administration of bivalent Streptococosis/Yersiniosis vaccine to the rainbow trout provided appropriate protection against disease and effectively induced the immune response against these two diseases, which is comparable to either of the vaccines separately.

Poultry diet contamination with cadmium (Cd) is both important in terms of economic losses due to reduced production and public health. This study aimed to evaluate the effect of turmeric powder (TP) containing a certain amount of curcuminoids (2.96%) on Japanese quail exposed to dietary cadmium. 180 male Japanese quail from 22 to 55 days of age were divided into 6 groups and received, basal diet(BD),  BD + 3 g/kg TP,  BD + 5 g/kg TP,  BD + 100mg/kg Cd, BD + 100 mg/kg  Cd and 3 g/kg TP, and BD + 100mg/kg Cd and 5 g/kg TP. Cadmium contaminated diet significantly decreases SOD, CAT, and total antioxidant capacity and increasing MDA concentration in liver tissue. Cadmium induced hepatic histological lesions, including fatty change, hyperaemia, single hepatocyte necrosis, and leukocyte infiltration. Turmeric powder substantially increased antioxidant status and decreased the severity of liver histological lesions in a cadmium-contaminated diet (P <0.5). When 5 g/kg of turmeric powder was added to the cadmium-contaminated food, the deleterious effects of dietary cadmium in Japanese quail were reduced more effectively.

Skeletal muscle resorption is caused by the biosynthesis of new structures involved in the muscle contraction in terms of the increased skeletal muscle cross-sectional area, which is recognized as one of the major muscle changes as a result of exercise. This study aimed to investigate the response of mTOR and AKT proteins to distinct eccentric and concentric resistance training in healthy subjects. Ten healthy men were randomly divided into two groups (5 patients in the eccentric group - 5 in eccentric group). Isokinetic contraction protocols included eccentric and concentric knee extension with maximum power and angular velocity of 60 degrees per second. The torques assigned to each subject were assumed to match the workload in both protocols, with a rotational speed of 60 degrees per second. Contractions consisted of a maximum of 12 sets of 10 repetitions for the right leg, a rest time of 30 seconds between each set. At the beginning and end of the study, biopsy of the lateral broad muscle tissue was performed. A biopsy was performed in both distal and proximal directions of lateral flat muscle. Tissue analysis using immunocytochemistry was used to assess the expression of mTOR and AKT proteins in each group. A dependent t-test and a covariance test were used to assess the data. For data analysis, SPSS21 and Exell 2013 software were utilized. The findings indicated that after one session of exercise, intra-group increases in mTOR and AKT proteins were significant in both eccentric and concentric groups (p ≤0.05).However, the inter-group changes of mTOR and AKT proteins showed no difference between two groups. In general, the present study showed that a session of eccentric and concentric activity leads to changes in the factors involved in skeletal muscle strength and hypertrophy. Besides, these changes in aggregate eccentric contraction are more than concentric.

Varicocele is one of the most important causes of infertility in men, which in addition to negatively affecting spermatogenesis, can also affect the endocrine system. The link between varicocele and testosterone production is unclear. The liver is one of the most effective organs in the metabolism of substances and hormones. This study aimed to evaluate serum testosterone levels and their association with liver enzymatic changes. Fifty male Wistar rats were divided into 5 groups, including sham control, varicocele induction, varicocele induction (50 mg/kg curcumin), and varicocele induction (100 mg / kg curcumin). After the treatment period, iron (Fe2 +), testosterone, direct bilirubin, total bilirubin and serum concentrations of ALT, and AST were assessed and liver tissue was isolated and examined. Varicocele caused a significant increase in iron levels (p ≤ 0.05) and a non-significant decrease in testosterone levels (p = 0.242) and total bilirubin (T) (p = 0.639). The treatment resulted in a significant decrease in the levels of iron (p = 0.056) and total bilirubin (p = 0.639), as well as a significant increase in testosterone levels (p < 0.05) compared to the varicocele. While the level of direct bilirubin (p ≥ 0.05) in the varicocele showed a significant increase compared to the sham and control. Using curcumin significantly reduced it compared to the varicocele (p = 0.03). Evaluation of ALT (p = 0.762) and AST (p = 0.367) levels did not show a significant difference among the groups. The levels of iron, testosterone, bilirubin, and liver enzymes are negatively affected by varicocele. Curcumin may lessen the harmful effects of varicocele on biochemical components linked with liver tissue in addition to preventing varicocele damage to testicular tissue. In varicocele rats, greater dosages of curcumin may lower the liver enzyme AST.

Despite a number of experimental studies, the healing effects of stem cells on chronic wounds still face serious challenges. The present study investigated the effect of injection of fibroblasts isolated from foreskin on the histological improvement of diabetic wounds in male rats. 24 male Wistar rats were divided into control and treatment groups during this experimental laboratory study.  Diabetes was induced in control and treatment groups using streptozotocin. Using a biopsy punch, a wound was created in the dorsal region of the animals Foreskin derived fibroblasts were injected into the dermis layer in the treatment group. On days 7, 14, and 21 after treatment, the wound healing was evaluated using morphologic observation, histological examination through hematoxylin- eosin staining, and measuring the wound size by Image j. On day 14 after treatment, the wound area was significantly smaller in the treated group than the control group (P<0.001). Histological examination showed that the skin thickness significantly increased in the treatment group on days 14 and 21 compared with control group (P<0.01). Besides, no morphologic complications were observed in the skin tissue following the injection of fibroblast cells. Our findings indicated that fibroblast cells are capable of accelerating the process of diabetic wound healing without morphologic complications in the skin tissue; according to which, the foreskin derived fibroblast cells can be used in the field of cell therapy.

This study aimed to investigate the fermentation efficiency of faecal microorganisms in comparison with rumen microorganisms to estimate the nutritional value of corn and sorghum grains in ruminants using in vitro gas production technique. To perform in vitro gas production technique with rumen liquor and faecal suspension, rumen fluid and fresh faeces were collected from three fistulated Gezel rams. The results of this study showed that there are no significant differences due to in vitro gas production between the two methods at different incubation times in experimental feedstuffs. There was no significant difference between two methods in terms of the amount of gas production from fermentable fraction (A) for corn grain, while the amount of this parameter by fecal suspension was significantly higher than that of rumen liquor in sorghum grain (P<0.05). Estimated metabolizable energy (ME), net energy for lactation (NEL), organic matter digestibility (OMD), and short chain fatty acids (SCFA) contents of experimental grains with rumen fluid and faecal suspension showed no significant differences.The amount of gas produced with rumen liquor can be successfully estimated from faeces suspension using the obtained equations Ysorghum=0.8929 X -1.6353 for the sorghum grain and Ycorn=0.9657X-3.4097 for corn grain. According to the results of the study, it seems that the animal faeces suspension has the potential to replace rumen liquor in the in vitro gas production technique for ruminants feed evaluation.

The present study aimed to investigate the response of MyoD and MRF4 myokines to eccentric and concentric activity in healthy young men. Ten healthy males were split into two groups of five, each with concentric and eccentric contractions. Excentric and concentric knee extension with a maximal power and angular velocity of 60 per second were used in isokinetic contraction protocols. The torque set for each subject was considered the same in both protocols to match the load, and the reciprocating speed was 60 degrees per second. At the beginning and end of the activity, the extensive biopsy was performed to examine the expression of the MyoD and MRF4 genes. The results showed that the intragroup changes of MRF4 after one session of activity were not significant in the eccentric (p = 0.229) and concentric (p = 0.178) groups. Furthermore, intergroup changes showed no difference between two groups (p = 0.182). Moreover, the changes within MyoD group after one activity session were significant in the eccentric group (p = 0.001) but not significant in the concentric group (p = 0.318). Moreover, intergroup changes showed no difference between the two groups (p = 0.821). In general, the present study showed that one session of eccentric and concentric activity leads to changes in the factors involved in skeletal muscle strength and hypertrophy. Besides, these changes were generally greater in the eccentric than concentric contraction. Therefore, it is recommended to focus more on concentric exercises to increase hypertrophy and muscle strength.

This study aimed evaluate the effect of short-term detraining following 6 weeks of high-intensity interval training on HIF-1α, bronchus, and bronchioles apoptosis levels on lung tissue in male Wistar rats. For this purpose, 18 healthy Wistar male rats (4 weeks with a mean weight of 72±9.9 g) were divided into experimental (n=12) and control (n=6) groups. After 6 weeks of high-intensity interval training, 6 rats the experimental group was transferred to the detraining group (n=6) and kept in detrain condition for three weeks. To measure of HIF-1α and bronchus and bronchioles apoptosis, a lung tissue sample was taken and measured at the end of the ninth week. One-way analysis of variance at the significance level (P≤0.05) was used to analyze the data. The results showed that despite the significant increase in HIF-1α and bronchial apoptosis and pulmonary bronchioles (P≤0.05) during high-intensity training, there was a significant decrease (P ≤ 0.05) in HIF-1α, bronchial apoptosis and bronchioles of lung tissue were observed. It seems to be done by the increase of the amount of HIF-1α and apoptosis in multiple cell lung bronchi and bronchioles, but the detraining period will decrease.
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The present investigation was carried out to evaluate the effects of dioxide titanium particles on growth and performance character. Based on previous works, it is expected that TiO2 nanoparticles (NPs) improving the feed efficiency and promote silk protein synthesis in the silkworm can be improved important traits such as cocoon shell weight and cocoon weight. The experiment was designed in a completely randomized design with three replications. Five groups of silkworms fed diets with dioxide titanium nanoparticles at 0, 5, 10, 50, and 100 mg/l in duration 4 and 5 instars. Data were analyzed by SAS software, and Duncan test was used to compare the mean. It was observed that the addition of dioxide titanium nanoparticles at different levels significantly improved percent viability, cocoon and cocoon shell performance compared control group.  In comparison to the control treatment, cocoon weights rose by 3.3 and 3.85 percent in 5 and 10 mg/l dioxide titanium, respectively. In comparison to the control treatment, 5 mg/l nanoparticles had a higher cocoon shell weight and cocoon shell ratio of 10.40 and 6.27 percent, respectively. Results showed that using dioxide titanium nanoparticles in 5 and 10 mg/l can increase cocoon and cocoon shell performance, but it has not effect on these traits with higher concentration.

Sustanon is considered an oil-based injectable anabolic-androgenic steroid. It comprises four different testosterone esters. The principal androgen in the testis is testosterone which is mediated by the androgen receptor. The current study aimed to investigate AR gene expression and biochemical and histological analyses in male rats after using various dosages of Sustanon. There were four groups of male adult rats. The first group (control) received an intramuscular injection of Sustanon solution (peanut oil). The second, third, and fourth groups underwent the treatment with a weekly injection of different Sustanon dosages of 10, 20, and 30 mg/kg for 8 weeks, respectively. The blood samples were obtained and the measurement of the serum levels of FSH and LH were conducted. The testes were extracted for histopathological and gene expression investigation. Using generated cDNA, real-time PCR was used to evaluate AR gene expression. SPSS software was used to do statistical analysis and analysis of variance on the data. Sustanon was shown to drastically lower the number of Leydig and Sertoli cells, perhaps resulting in a large drop in FSH and LH concentrations, according to the results.The expression of AR was dose-dependent and significantly increased at a dose of 10 mg / kg and decreased significantly at a dose of 30 mg / kg compared to the control group. Sustanon is distinguished by its unique structure. It induced biochemical plus histopathological damages and changed AR gene expression in adult male rats that may cause infertility.

Millions of people worldwide have Alzheimer's disease, and with the disproportionate growth of the elderly population, the disease, which is the most common form of dementia among the elderly, is becoming a public health crisis. This study aimed to investigate the effect of endurance training before and after Alzheimer's induction on some neuroplasticity-related factors in the hippocampus of male Wistar rats. Before inducing Alzheimer's disease, 32 8-week-old male rats weighing an average of 250 g were randomly allocated into two groups: rest (16 heads) and exercise (16 heads). Each group was divided into two subgroups after four weeks (the first and second weeks at a speed of 10 meters per minute in two 15-minute shifts, the third week at a speed of 15 meters per minute in three 15-minute shifts, and the fourth week at a speed of 15 meters per minute in four 15-minute shifts with 5-minute intervals).1. amyloid beta injection and 2. without injection. After 72 hours, the animals were killed and their hippocampus was removed for examination. Real Time PCR measured BDNF, PKG, and cGMP gene expression.Data were analyzed by one-way analysis of variance and Tukey post hoc test at the significant level of p <0.05. The results showed that the levels of BDNF, PKG and cGMP in the exercise group were significantly higher than the rest group in the stage after Alzheimer's induction (p = 0.001). According to the results, it seems that aerobic exercise can help improve neuroplasticity in the hippocampus of Alzheimer's mice.

Using plant growth supplements in the diet of broilers increases the production efficiency. The purpose of this study was to compare the effects of different amounts of sumac powder on broiler chicken growth, gastrointestinal development, immune system, and blood factors. 160 one-day-old male Ross 308 chickens were used in a completely randomized design with four treatments and four replications, each with ten chickens.The experimental diets were: 1) control with basal diet (no sumac), 2) basal diet containing the 0.25% sumac 3) basal diet containing the 0.75% of sumac, and 4) basal diet containing 1.5%. The results showed that the increase in body weight and feed conversion ratio during the rearing period in the diet containing 1.5% of sumac powder significantly improved (p <0.05). The weight of Fabricius bursa in all different levels of sumac powder significantly increased compared to the control (p <0.01). There was a significant increase in hematocrit percentage for the treatments containing levels of 0.75 and 1.5% of sumac powder (p <0.05). The amount of 1.5% sumac powder in the diet had a significant improvement on the concentration of albumin, cholesterol, and very low-density lipoproteins (VLDL) in the serum of broiler chickens (p <0.05). In general, the results showed that using sumac powder in the diet of broilers could have beneficial effects as a growth stimulant.
 
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Stress is a neurological condition that affects the nerves of all organisms by molecular processes. Magnesium oxide nanoparticles effectively reduce and relieve anxiety-like behaviors, but the molecular mechanism of this relationship has not yet been studied. Gad2 gene encodes one of the isoforms of glutamate decarboxylase, an enzyme which converts glutamic acid to GABA (gamma amino butyric acid). In the present study, the effect of magnesium oxide nanoparticles on Gad2 gene expression in the hippocampus of male rats in the presence and absence of acute motor stress was investigated. 4 groups of male Wistar rats( control, receiving salin+stress, 5mg/kg Mg nanoxide, stress+ 5mg/kg Mg nanoxide) were prepared. RNA was extracted from hippocampal tissue. Then, cDNA synthesis and real-time were performed for all groups. The results showed that Gad2 gene expression was significantly (p<0.01) increased in the presence of magnesium nanoxide, especially in the group with acute motor stress compared to other groups. According to the findings, magnesium nanoxide enhances the inhibitory nervous system by enhancing the hippocampal enzyme glutamate decarboxylase. Additional research in this area will throw more light on this molecular connection.

Gold nanoparticles disturb the physiological processes of the liver tissue by affecting the body’s biological system. Given gingerol's antioxidant properties, researchers wanted to see how it affected the levels of liver enzymes and oxidative stress indices in the liver tissue of rats exposed to gold nanoparticles.This experimental research was conducted on 32 male Wistar rats, divided into four equal groups of control, gold nanoparticle (one administration of 0.5 ml gold nanoparticle, 200 ppm and 60 nanometers), gold nanoparticles + 50 mg/kg concentration of gingerol, and gold nanoparticles + 100 mg/kg gingerol. At the end of treatment period, the serum level of ALT, AST, GGT, ALP, and level of antioxidant enzymes of SOD < GST, and CAT, and levels of MDA and HOdG-8 in the liver tissue were measured using the ELISA method. In this study, rats receiving gold nanoparticles with concentrations of 50 and 100 mg/kg gingerol had significantly higher levels of SOD, GST, and CAT enzymes in their liver tissue than the dose-dependent group receiving gold nanoparticles. The serum levels of ALT, AST, GGT, and ALP, on the other hand, were reduced. Furthermore, the levels of MDA and HOdG-8 in rat liver tissue were found to be significantly lower (P <0.001).According to the results of study, gingerol improved the oxidative stress indices in liver tissue and decreased the serum level of liver enzymes. Therefore, this compound can be exploited to reduce DNA oxidative stress and lipid peroxidation in liver tissue.

Glioblastoma is one of the most dangerous types of brain cancer, with a high rate of therapy resistance. Apoptosis, angiogenesis, autophagy, NF-κB, and Wnt pathways are just a few of the molecular and cellular processes that play a role in Glioblastoma development. The effectiveness of curcumin and Nano-micell curcumin with Erlotinib to suppress Glioblastoma in vitro was investigated in this study.The suppression is carried out by affecting NF-κB and Wnt signaling pathways, angiogenesis inhibition, and autophagy and apoptosis induction. Curcumin and Nano-micelle Curcumin (50 μM) was investigated alone and with Erlotinib (50 μM) in the U87 glioblastoma cells. The expression of Wnt and NF-κB signaling pathways, apoptosis, angiogenesis, and autophagy-related genes and proteins were assessed by qRT-PCR and Western blot. Compared with the control group, all treatments declined the U87 glioblastoma cells viability. Furthermore, Angiogenesis-associated proteins, i.e., Cox-2, VEGF, HIF-1α & bFGF, were remarkably decreased. Each treatment regulated autophagy and apoptosis-associated proteins, i.e., Bax, Beclin 1, caspase 8, Bcl-2, LC3-II, and LC3-I. Total NF κB (p65) and phospho NF. κB (p65) declined by each treatment at protein levels. Expressions of VEGF, cyclin D1, Twist, ZEB, and Wnt pathway-associated genes were also decreased. In general, our findings demonstrated that curcumin and Nano-micelle Curcumin, either alone or in conjunction with Erlotinib, had anti-Glioblastoma effects via modulating a number of processes including apoptosis, autophagy, angiogenesis, Wnt, and NF. κB signaling pathways.

Cancer causes about 13% of human deaths and has not yet been properly treated. Conventional cancer treatment methods such as surgery, chemotherapy, and radiotherapy have many side effects and in some cases have no effect on cancer treatment, so creating appropriate and targeted therapies can revolutionize cancer treatment. New methods include gene therapy and using immunotoxins, which can act up to a high percentage. This study aimed to investigate the effect of modified PA protein that binds only to cancer cells along with lethal factor (LF) on cancer cells. In this regard, recombinant mPA and LF proteins were isolated from recombinant Bacillus subtilis and after examining the presence of protein bands and their concentrations using SDS-PAGE and Bradford methods, respectively, the combined effect of different concentrations of these proteins on lung cancer cells, Breast, prostate and pancreas were examined by MTT method. The results showed that 75 ng of recombinant LF protein and 50 ng of recombinant mPA protein had the greatest effect on cancer cell death and caused the death of more than 98% of the studied cancer cells. With these results, it is hoped that a new dimension can be added to cancer treatment by producing targeted toxins such as mPA and LF.