ارسطو روشن

Background and Hydatidosis is highly prevalent worldwide which mainly involves liver and lung. There are some drugs sensitive to the hydatid disease but surgery is still the most common form of treatment. Different chemical agents are used for inactivation of protoscolices during surgery but most leave serious side effects. Therefore, herbal extracts have received more attention as replacing agents that have acceptable scolicidal effects and no adverse effects. The aim of this study was to investigate the scolicidal effects of mixture of Artemisia, Eucalyptus and ginger extracts on hydatid cyst protoscolices in vitro.
Hydatid liver cysts isolated from sheep were collected from a slaughterhouse in Hamedan, Iran. The cysts fluid containing live protoscolices were aspirated aseptically. The effect of two concentrations of the methanolic extracts of the mixture (50 and 100 mg/ml) was investigated at 15 and 30 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Data analysis was done applying Chi-square test.
The mixture of eucalyptus and ginger extracts showed acceptable scolicidal effects in which after 15 and 30 min exposure at 50 and 100 mg/ml concentrations it killed 97.24% and 100% of protoscolices, respectively.
This study showed that mixture of Eucalyptus and ginger had high scolicidal activity in vitro condition. Therefore, it could be used in surgical treatment of hydatid cyst after complementary researches.

Hydatidosis is one of the dengerous zoonotic diseases that cause serious problems for human health, as well as major economic losses for livestock industry. Due to the nature of the parasite life cycle and also the structure of the cyst in human, the control of parasite in community is difficult and its treatment has faced with a major challenge. One of these challenges is inactivating the protoscolices for treatment purposes and preventing secondary cysts. Different chemicals have been used in the treatment of cyst that most of them had serious side effects for the patient. The aim of this study was investigating the scolicidal effects of some herbal extracts in vitro. Liver hydatid cysts were collected from slaughterhouse; the cysts fluid containing live protoscolex was aspirated aseptically and stored at 4°C until use. Three concentrations (25, 50 and 100 mg per ml) of each extract (ginger and artemisia) prepared and protoscoleces placed into incubator at 37oC. The viability of the protoscoleces was determined by eosin staining method at the times 5, 10, 25, 40 and 60 minutes. The methanolic extract of ginger at the concentration of 100 mg/ml leads to kill all of protoscoleces at 40 minutes. While the artemisia extract in none of investigated concentrations had not much effect on the protoscoleces. The study of animal models and complementary tests showed that methanolic extract of ginger can be used as an effective protoscolex for it has high activity.

Introduction & 130 heteroxenous species of sarcosytis with different life cycle and pathogenesis have been recognized. The pathogenic species for humans are S. hominis from cattle and S. suihominis from pig that humans are definitive and cattle and pig are intermedi-ate hosts. Some species of Sarcocystis can cause important economic loss and disease in livestock، and health issues in humans. The aim of this study was to determine the prevalence of Sarcocystis infection in slaughtered Cattle and sheep in Hamadan، west of Iran. In this cross sectional descriptive study a total of 324 cattle and 334 sheep carcasses were examined using naked eye inspection for macroscopic Sarcocysts، and digestion method، for microscopic types of parasite. Muscles from thigh، heart، tongue، esophagus، diaphragm and costal muscles were examined. All carcasses examined by naked eyes and tissues were minced and poured in digestion medium separately and sediment was examined microscopically. The prevalence of microscopic Sarcocystis in cattle was detected in 100% and there was no macroscopic cyst in examined carcasses. However، the prevalence of microscopic Sarcocystis in the sheep was also 100% and the sarcocysts were found in the 48. 34 % of esophagus and 29. 49% of diaphragm muscles by naked eyes inspection. The digestion is found the most sensitive method for diagnosis of Sarcocystis. Al-though 100% of muscles were found infected but the majority of the cysts in the sheep and all in the cattle were as microcysts. That means، the meat should be cooked sufficiently irrespec-tive of meat inspection results.

Cysticercus tenuicollis is the larval stage of Taenia hydatigena, which causes great economic losses to the livestock industry. The aim of this study was to evaluate the prevalence and health care Importance of cysticercus tenuicollis larva in livestock animals slaughtered in industrial slaughterhouse of Hamadan based on age and sex in 2015. This descriptive analytical cross-sectional study was carried out with simple sampling from slaughtered livestock in industrial slaughterhouse of Hamadan Using macroscopic observation method. For determination of prevalence of Cysticercus tenuicollis and its seasonal changes, about 6000 slaughtered livestock in slaughterhouse were studied and their sex and age were recorded. Data analysis was performed using spss-20 statistical software, descriptive statistics and Chi-square test. In this study the frequency of cysticercus infection was 14% in cattle, 28.5% in sheep and 10% in goat. All together the frequency of infection in slaughtered livestock was 17.5%. The rate of Infection in females was more than males (p=0.02). There was a statistical significant difference between the rate of Infection and seasons of the year (p

SummuryTaniasis, caused by Tania saginata, is one of the common diseases between human and cattle. Human infection with adult worms and larval stage of the parasite in cattle was reported from different regions of Iran. The aim of this study is description of the rate of infection and health care importance of the cysticercus bovis infection in cattle in Hamadan industrial slaughterhouse in 2014.
This is a cross sectional –analytical descriptive study. In this study the presence of C. bovis in the scapular muscles, heart and thigh, between the ribs and diaphragm and liver of the cattle were surveyed. Both alive and calcareous cysts were studied. Data were analyzed using SPSS- 21 software, descriptive statistics and chi-square test.
The prevalence of C. bovis in 2000 studied cattle was 3.8%. The highest rate of infection was found in heart muscle and there was a statistically significant difference between the rate of infection in different organs (p