فهرست مطالب حسن روان سالار

One of the common adulterations in milk and dairy products, is the addition of low-priced vegetable oils such as palm oil in order to increase the profitability of products. Therefore, there is an urgent need to develop accurate, fast and reliable identification techniques to protect consumers against this type of adulteration. The aim of the present study was DNA barcoding with real-time PCR as a reliable method for detecting palm oil fraud with levels of 2, 3, 4, 5, 10, 30, 50 and 100 % and positive and negative control samples in yogurt. According to the results, the highest and lowest concentrations of DNA extracted belonged to the palm oil sample of 100 % and 3% , respectively, while among all samples, the concentration of DNA extracted in sesame oil and then in the control oil sample was lower. It was determined from other samples. The lowest and highest adsorption rates (10mm) between the samples were control oil (excluding palm) and 100 % palm oil, respectively. There was no significant difference in absorption rate between 4%, 5% and sesame oil samples. The range for detecting palm oil adulteration was from 0.05% to 100% palm oil. LOQ and LOD were 0.1 % and 0.05 % of palm oil, respectively.

The members of the genus Mycobacterium, are the causative agents of mycobacteriosis, Mycobacteriosis is a chronic and progressive disease that may affect all tissues of the fish. Clinical signs include scale loss, dermal ulceration, spinal defects and etc. Mycobacteriosis is a zoonosis disease, and most people who deal with fish, are at risk. Due to the expansion of Rainbow fish breeding in the city of Sabzevar and having awareness the disease caused by Mycobacterium in fish and as a result of mortality and economic damage and also possibility of transferring this bacterium from fish to humans, it is necessary to study Mycobacterium in Sabzevar Rainbow fish. The purpose of this research is isolation and molecular identification of Mycobacterium from Rainbow fishes in Sabzevar pools. In this research 50 Rainbow fishes with clinical signs collected from seven different fish breeding pools in Sabzevar city. After autopsy and culture of the specimens in sterile conditions, in order to confirm acid-fast of the bacteria, was performed Ziel-Neelsen staining. Then, DNA extraction was performed and PCR-16s rRNA was performed to confirm mycobacterium infection. To determine identity, the results of the sequencing of the PCR product were evaluated using the BLAST program. In this research, three acidfast isolates grew, that the sequence of all three isolates belonged with 99% similar to Mycobacterium peregrinum. Given that M. peregrinum is isolated from aquatic and human respiratory tract infections, Therefore, it is necessary to warn people who deal with fish in any way.

The aim of this study was to investigate the effects of two types of quran voice، Majlisi or Tartil on IgA، IgM، IgG، C3، C4 and White Blood Cell count. Methods and Materials: this study was conducted on 60 volunteer students in sabzevar university of Medical sciences. Subjects were divided randomly into 2 groups. In the first group، participants heard to Majlisi voice and in the second group، subjects heard to Tartil voice of Quran for 20 minutes. Blood sampling were done before and after Quran hearing. IgA، IgM، IgG، C3 and C4 were measured by SRID and kit method and white blood cell count was done by cell counter. Data were analyzed using SPSS16 software and U Mann – Whitney and Kruskal Wallis tests. Hearing the Majlisi voice significantly increased the IgG (p=0. 032)، C3 (p=0. 049) and C4 (p=0. 004)، but it did not change other factors. Hearing the Tartil voice rose significantly gust the IgG (p=0. 008) and C3 (p=0. 001). The results of this study shows that hearing to both types of Quran voice (Majlisi or Tartil) can go up the immune system factors and inhance this to encountering some stressful and critical situations.

Background and Thyroid autoimmune disease is the most common thyroid disorder in low-iodine intake areas; evaluation of thyroid peroxidase antibodies (TPOAb), anti-Thyoglubolin (TGAb) and anti-microsomal (TMAb) in most patients with hypothyroidism is of significant importance. Therefore, the present study was conducted to determine the role of anti-thyroid antibodies in patients with hypothyroidism in Sabzevar, Iran. Methods and Materials: This is a cross-sectional descriptive analytical study where 382 subjects with TSH above normal and T3 and T4 below normal were selected through non-probability convenient sampling. Their TSH, TPOAb, TGAb and TMSb were measured through Eliza method. The obtained data were analyzed in SPSS 15 using chi-square, independent t-test and Pearson correlation coefficient, where P<0.05 was considered significant. TPOAb, TGAb and TMSb in men and women with TSH higher than 10 mIU/ml were 72.3%, 78.3% and 50% positive respectively. In 72.3% of the subjects, TPOAb was above normal; in 78.5% of them TMAb was higher, and in 50% of them TGAb was higher. The highest rate of correlation was observed between TGAb and TPOAb (r=0.89). The results indicated that, in the population studied, TPOAb, TMAb and TGAb were higher than the normal rates, and that TPOAb and TGAb are more remarkably changing to higher rates than TMAb.

Background and Vulvovaginal candidiasis is one of the most common infections in women and is caused by various types of candidiasis with different patterns of resistance against drugs. The present study was conducted to determine the various species of candidiasis in women admitted to Mobini Hospital in Sabzevar, Iran in order to make preventive recommendations for medical doctors. Methods and Materials: This cross-sectional descriptive analytical research was conducted on 231 women admitted to Mobini Hospital in Sabzevar, Iran, complaining of vaginal secretions. After getting the ethics committee permissions and obtaining the informed consent of the participants, at the same time as measuring vaginal pH, two swaps were used for collecting samples of secretions from lateral and posterior fornix. They were examined for yeasts and were then cultured in saburu dextrose. Grown yeasts were detected based on their macroscopic and microscopic features, tubulation in blood serum and biochemical characteristics using Yeast Plus System. The obtained data were analyzed in SPSS 11 using chi-square and bi-dimensional tables. Positives results in direct examination results were 7.35%, and in culture the positive results were 26.8%. In detecting processes, candida albicans yeast (38.7%), Candida kefyr (17.7%), Saccharomyces cerevisiae (14,5%); candia tropicalis (9.6%), Candida glabrata (8%), candida cruse (3.2%) were isolated with other non-pathogenic yeasts including Candida rugosa, Candida lipolytica, trycospron bejli, Blastoschizomyces capitatus (6.4%). Non-pathogenic yeasts were isolated from patients with defective immunity systems, and need to be considered in patients with vaginal infections.