سید مصطفی عقیلی نژاد

The aim of this study was to investigate the effect of in fall and winter on the hematological and serum biochemical factors of 3-4 years old great sturgeon in the penclture ) located in the Gorgan Bay (Caspian sea) and processing sturgeon center of Marjani for 6 months.  Huso huso individuals were anesthetized withclove powderand 5 mL of bloodwas sampling from a total of 15 great sturgeon specimens were apparentlyhealthy by caudal venous puncture each of the examined panes (3 pins)in different seasons.Blood was analyzed with routine method and standard formulas used in fish hematology. the results showed thatthere are no any differences between the mean corpuscular volume, cortisol levels and activity of Alanine aminotransferase enzyme in the different season(p>0.05).But, by examining other parameters, the highest WBCs number, Activity of aspartate aminotransferase and alkaline phosphatase enzymes in summer, the highest RBCs number, hematocrit concentration, total serum protein in spring, the highesthemoglobin concentration, the mean corpuscular hemoglobinand themean corpuscular hemoglobin concentration in autumn and the highest glucose levels in winter were observed, which were significantly increased in comparison with other seasons (p<0.05). Overally, based on these results, it can be rectified that temperature changes as a result of season transition may have an important role on Blood factors of Huso huso. As with the same ecological conditions, with changing the temperature of the environment, we will see different changes in the blood factorsin the Beluga.

To investigate the relationship between the length and weight of Neogobius melanostomus, 5145 fish were captured using nets from March to February 2018. The maximum total length and weight for females were recorded at 12.2 cm and 28.08 grams, while for males, the maximum total length and weight were 11.3 cm and 33.17 grams, respectively. The length-weight relationship for both female and male populations of N. melanostomus in Gorgan Bay across the four seasons (spring, summer, autumn, and winter) showed a high coefficient of determination. The regression slope values (b) for this species in different seasons were significantly different from the isometric growth coefficient of 3 (p < 0.05). Specifically, the t-values were 3.73 for the winter population, 10.80 for the autumn population, 21.25 for the summer population, and 21.71 for the spring population, indicating positive allometric growth throughout the year. The Pauli test confirmed this growth pattern in the N. melanostomus population of Gorgan Bay. Seasonal condition coefficients showed that females had the highest values in summer and winter, while males displayed the highest values in winter.

This study aims to analyze the fishing trend and predict the future catch of Persian sturgeon (Acipenser persicus) stocks by DB-SRA and dynamic models in the southern shores of the Caspian Sea. The results showed that, according to the size of the initial population in 2018, equal to 153 spicemens(mean weigth 30kg); assuming a constant rate of catch from the population equivalentto 0.2 per year (half of the current catch); until the next 30 years (2050), the amount of catching of Persian sturgeon will increase until the stocks of this species have the ability to fully restore themselves. While assuming the current catch rate, there has been a sharp decrease in the catch of fish in the southern shores of the Caspian Sea; it is estimated that the stocks of this type of sturgeon fish will be extinct by 2050

The present study aimed to investigate variability in growth pattern and evaluate abundance of A. persicus based on catch data in the southern Caspian Sea water in a period of 27 years (1991-2018). The data of 72704 specimens were analyzed separately by sex (male, female and population), time (years) and region (coastal waters of Gilan, Mazandaran and Golestan provinces). The highest catch of this species was recorded in 1991 (6038 pieces of the fish) and the lowest catch was recorded in 2018 (292 pieces). During the period, the Golestan province had the highest number of catches (30851 pieces) and Gilan province had the lowest ones (11129 pieces). The maximum recorded fork length of this species was 240 cm in 2006. The results showed that the highest annual average fork length was 151 cm in 2007 and 2010 and the highest annual average total weight in 1993, 1994, 1996, 1997 and 2007, which was 26000 grams. The lowest annual average fork length was 139 cm in 2018 and the lowest annual average total weight was 600 grams in 2010 and 2012. The highest length frequency corresponded for the ranges of 136-148 and 148-160 cm in males and females, respectively. The females showed the highest catches in 1991, and the lowest in 2018, while the males had the highest in 1991, and the lowest in 2014. The females dominated the population in the period of 1991- 2013, and after that this ratio was reversed. The allometric coefficient of growth (b) varied between 1.03 and 3.49 in the study period. The growth pattern was mostly positive allometric in first decade of study (1991-2001), isometric or negative allometric in the second decade of study period (2001-2011), and negative in third decade (2011-2018). During the study period, the condition factor of the fish varied between 0.61 and 0.80, and the average of the factor for the whole period of study was 0.72. Generally, the catch of A. persicus in the southern Caspian waters decreased more than 95% from 1991 to 2018 (a period of 27 catch-years).

Variability in growth allometry of two prawn species Palaemon adspersus and Palaemon elegans was investigated in the Gorgan Bay, SE Caspian Sea, during a period between May 2019 and April 2020. Resalts showed that females grew to a larger size than males in both species. The weight growth of females was faster than length growth in P. adspersus (Log TW = -5.3975 + 3.2018Log TL), while in P. elegans the length growth of males was faster than weight growth (Log TW = -4.9019 + 2.8758Log TL). In P. adspersus TL – CL relationship was CL = 0.2162TL + 0.002 for females and CL = 0.2087TL + 0.0096 for males. In P. elegans TL – CL relationship was CL = 0.2451TL – 0.0908 for females and CL = 0.2043TL + 0.0372 for males. TL – CL relationships were nagative allometry in both sexes of two species. Mean condition factor (CF) of females was larger than that of males in both species, and it was observed significant difference in the mean condition factor between species and month.

This study aimed to investigate the changes and growth pattern of Beluga sturgeon fishing in fishing regions of Golestan and biological characteristics of this species over a period of 13 years. The highest amount of Beluga sturgeon fishing was in 2008, when a total of 95 pieces of this species were caught from five fishing grounds in Golestan province, and the lowest amount was caught in 2020, when 3 pieces of this species were caught. The results showed that the highest average total length was observed in 2020 and the highest total weight was observed in 2017 and the lowest average total length and total weight was obtained in 2011.The sex ratio of male to female elephant fish in the thirteen-year period was 0.66:1, which was significantly higher than the female population (X2=19.69, p<0.05). The range of growth coefficient (b) for the population of Beluga sturgeon between 2008 to 2020 years varied from 2.56 to 3.97. The results of Pauli test for the relationship between length and weight of Beluga sturgeon species showed that the growth pattern of this species in 2008 and 2010 were negative allometric, in 1393, 1396 and 1397 positive allometric and in 2009, 2011, 2012, 2013, 2015, 2016, 2019 and 2020 were isometric. In general, in a period of 13 years, the amount of elephant fishing in the coasts of Golestan province decreased by more than 90%, so in this region, this trend for Beluga sturgeon is among the most endangered species, concluding an alarm for conservation of this valuable species in Caspian Sea..

This study aimed to investigate the changes and growth pattern of Persian sturgeon fishing in fishing regions of Golestan and biological characteristics of this species over a period of 10 years. The highest amount of Persian sturgeon fishing was in 2012, when a total of 143 pieces of this species were caught from five fishing grounds in Golestan province, and the lowest amount was caught in 1398, when 49 pieces of this species were caught. in the present study, the maximum total length of Qarabrun was in 1397 with a total length of 257 cm. Mianqaleh fishing farm had the highest number of catches (471 pieces) of this species and the lowest amount of catches (26 pieces) for this species was recorded in Chalasht fishing farm. The results showed that the highest average total length was observed in 2014 and the highest total weight was observed in 2017 and the lowest average total length and total weight was obtained in 2012. The sex ratio of males to females of Persian sturgeon species in the ten-year period was 1.24: 1, where the male population was higher (p < 0.05). The range of growth coefficient (b) for the population of Persian sturgeon in the years 2011 to 2020 varied between 2.60 to 3.82, The results of Pauli test for the relationship between length and weight of Persian sturgeon species showed that the growth pattern of this species in 2011 and 2012 was negative allometric, in 2015 positive allometric and in 2013, 2014,  2016, 2017, 2018, 2019 and 2020 It was isometric. Conclusion of this study shows that in a period of 10 years for Qarahbrun species had a decrease of 75%, so in the waters of Golestan province this trend for Qarahbrun is among the most endangered species and this means an alarm for this valuable species It has been heard in the Caspian Sea.

The bony fishes of the Caspian Sea are the species whose reserves are decreasing due to Pollution, habitat destruction, exploitation and illegal fishing. Regarding the extent of illegal fishing in Golestan province and lack of sufficient studies in this regard, this study examines the changes in illegal fishing and identifies the factors involved in its occurrence. Using descriptive-analytical method, designing a questionnaire and questionnaire in person, the factors affecting the incidence of illegal fishing in the southern part of the Caspian Sea, Golestan province, were identified and ranked in two regions of Turkmen and Gomishan. Required information was collected by completing 50 questionnaires and questions related to social, economic and hunting causes during a one-year period (1395) was answered. The results showed a significant difference in illegal fishing between Bandar-Torkman and Gomishan (p ˂0.05, t- test= 3/3). Also, there was a significant difference between the winter and the rest of the seasons in the illegal fishing survey (p ˂0.05, ANOVA= 2/15). Analysis of illegal catch data by using linear regression model showed that there was a significant correlation between the probability of illegal catching of all trapped bones (in kilograms) and independent variables, including information from fish, establishment of trapping in sea, total number of traps, The main motive for hunting and the distance of the sea. This study showed that the rate of catches and reserves of bony species in the Caspian Sea has decreased, which has been accompanied by an increase in illegal fishing activities.

The purpose of this study was to evaluate the number, catch composition, and catch per unit effort (CPUE) of sturgeon spawners by gillnet in the fishing grounds of Golestan province. Sampling and data collection were conducted in the 4 fishing grounds according to the fishing seasons of sturgeon fish using gillnets (Polyamide) with a mesh size of 150 mm from autumn 2018 to autumn 2019. Five species of sturgeon fish including Acipenser persicus, Huso huso, Acipenser gueldenstaedtii, Acipenser nudiventris, and Acipenser stellatus were caught during the sampling period. The highest fish weight was observed in autumn 2019 (946 kg) and the lowest was in spring 2019 (242 kg). The highest total catch weight and fishing effort were obtained for matured males of A. persicus (389 kg and 0.9 g/fishing effort) and the lowest was for matured females of A. stellatus (13 kg and 0.03 g/fishing effort). According to the results of the Chi-square test, the separate and simultaneous effect of sampling time and the location was significant on the number of matured Acipenser persicus (P<0.05).

The Caspian Sea is valuable habitat ecologically and commercially for sturgeon fishes but their stocks severely decreasing for increasing illegal catches. This research was achieved different illegal fisherman activities and their motivation in the south east of Caspian Sea. In this study, data was collected from 203 illegal fisherman using analytical-description methods and questionnaire design with researcher in cities and villages on coast of south east of Caspian Sea. The reliability of the questionnaire based on Cronbach's alpha was 0.71. The results showed that most of the illegal fishing was in spring and autumn and most of fisherman with education low level and unemployed. Their method of fishing is the use of gill net, and they come even from further cities and villages for illegal catch. Their motivation was not related to age but decreased significantly with increasing education low level. There was a low, negative, and significant relationship between estimated motivation with answering to motivation question (r=-0.25**). Totally, preventing illegal supply and sale of fish in the province and identifying and responding to sales agents, illegal fishermen, increasing education and raising awareness of protecting valuable fish can reduce illegal activities.

Caspian sturgeon is one of the most important and economically valuable fish that unfortunately have witnessed a sharp decline in their natural stocks in recent decades, as they are on the endangered species list. Therefore, given the importance of sturgeon, identifying the factors that reduce their stockpiling is essential. The present study was applied in terms of purpose type and descriptive-analytical and survey method. The sample size was estimated based on Cochran formula of 270 fishermen by available and voluntary sampling method and the required information was collected by distributing questionnaires based on Likert spectrum and in-person interviews among fishermen in Golestan, Mazandaran and Guilan provinces. Questionnaire data analysis was performed using structural equations and PLS software and the findings indicated that factors such as overfishing, low release rate, illegal fishing, ؛ Inadequate fishing management ؛ Non-standard fishing gears and equipment had a significant and positive impact on the Reduction of sturgeon stocks , with the largest impact being illegal fishing. However, the effect of contaminant inflow and water pollution had a positive effect on the downward trend of sturgeon stocks, but was not significant. The findings of the present study can be of great help to fisheries decision makers in planning and Their implementing measures to restore sturgeon stocks their reserves and prevent their rapid decline and lead to improved sturgeon restoration efforts.

Golden gray mullet, Liza aurata is one of the most valuable and sensitive species in the Caspian Sea. Given the possibility of catching Caspian bony fish only in the second six months of each year, the present study was conducted from autumn to winter 2015 in the Caspian Sea and Gorgan Bay to investigate the effect of season and habitat on hematological and biochemical analysis of golden mullet fish serum. 30 golden mullet fish that were caught in both seasons randomly from the Caspian Sea and Gorgan Bay with a blade net were studied. The blood study was divided into two sections: blood serum studies (liver enzymes, cortisol, glucose and protein) and hematological indices (blood and differential cells). The results of enzymatic indices showed that all indices had the highest amount in the autumn seasons and the lowest in the winter, indicating a greater influence of the season on sampling location. The results of hematological, immunological and biochemical indices showed that these indices did not follow the same trend and did not adhere to a single characteristic and each index showed different responses under different environmental conditions and seasons. Overall, the results of this study confirm that the blood and serum parameters of mullet fish in different environmental and time conditions have different responses and among these parameters, the enzymatic parameters showed significant trend and could be suitable biomarkers.

The aim of this study was to determine the effect of Ultra-Amax perboitic on 1 g / kg diet Morphometric characteristics, growth performance and nutrition indices in adult male and female Huso huso for 120 days. For this purpose, 60 fishes with an average weight of 4.24± 0.27 kg after one week's adaptation They were randomly divided into 2 treatments and each with three replicates (with 10 replicates per each). The results showed that ultra-Amax prebiotics produced a significant difference in morphometric factors. But it did not affect the parameters such as Fork length and body length in the pectoral Fin. However, adding 1 g / kg of ultra-Amax perbiotic to fish diets Improved growth and nutrition parameters, specific growth rates, relative weight rates, daily growth rate, feed conversion ratio and relative eating were improved, However, no significant difference was observed between treatments   (P>0.05). The amount of obesity and nutritional conversion efficiency indicated a significant difference between treatments with control treatment. Based on the data analysis, it has been shown that commercial ultra-Amax is a more effective and effective effect on Adult male Huso huso. The total results of this study showed that dietary supplemented diets with ultra-Amax of 1 g / kg diet Can be effective in improving morphometric factors and growth performance and nutritional indices.

This experiment was conducted to evaluate some of the hematological (RBC, WBC, MCV, MCH, MCHC, Hb, PCV) and serum biochemical factors (TSP, GLU, COR, AST,ALT and ALP) of 2 to 3 year’s old Great Sturgeon during the yearlong period (autumn 2015 to summer 2016) at the Sturgeon Institute, Shahid Marjani in different seasons. For this purpose, after the seasonal blood sampling a total of 15 great sturgeon specimens were apparentlyhealthy after bleeding in the caudal vein hematological and serum biochemical indices was determined by standard protocols. Based on the results, seasonal variations were observed in WBCS, PCV, Hb, MCV, MCH, MCHC values, activity of AST, ALP enzymes and glucose and cortisol levels (P<0.05). But, there were no any significant differences between RBCs number’s, TSP levels and ALT values (P>0.05). Based on the results and according to the stable of all environmental conditions, except for temperature, couldbe claimed that temperature changes can be very important as a key factor in determining of hematological and biochemical indices of Huso huso in the different season that were complementary to the changes in water quality parameters indicating that environmental changes affects the blood chemistry of Huso huso. Overally, the hematological and biochemical values of the present study can be used as reference for monitoring of physiological and health status of Huso huso from Sturgeon Institute, Shahid Marjani and other center of the fishing and processing sturgeon fishes.

The aim of this study was to investigate the effects of different levels of Celmanax commercial prebiotic on growth performance, nutritional efficiency, blood and plasma biochemical indices in beluga, Huso huso (Linnaeus, 1758) in a 120-day feeding period. So that, 120 individuals of beluga with the average weight of 4.66 ± 0.26 kg were transferred to 12 holding cage nets, 1.20 × 1 × 2 m in size at a depth of 1.5 m with a completely randomized design as four treatments with three replications including control (without prebiotic) and three experimental treatments fed by diets containing 0.5, 1, 1.5 g/kg prebiotic. The results exhibited that adding Celmanax to the diet, especially 0.5 g/kg, significantly enhanced relative weight, daily growth rate, specific growth rate, feed conversion ratio and relative eating (p<0.05). However, no significant effect was observed in the protein and fat ratio (p>0.05). There were also no significant differences between the treatments fed by prebiotic compared to control with respect to hemoglobin, mean corposcular volume and mean corpuscular hemoglobin (p>0.05). Analyses on biochemical indices indicated that there were significant differences in total protein, glucose and triglyceride in blood plasma levels. Totally, according to the results, 0.5 g/kg Celmanax in diet can effectively enhance growth performance, nutritional efficiency and blood indices in cultured beluga.

Excessive exploitation of commercial aquatic resources, especially valuable Caspian sturgeons, has exposed these living resources to high extinction risks. This study was undertakenusing field-based surveysacross fishing zones in Golestan province and by designinga questionnaire. Data were collected through the distribution of 172 questionnaires among fishermen communities to answer questions related to social, economic, fisheries and conservation factors which are likely to contribute in the occurrence of illegal fishing. Following the evaluation of validity and reliability of the questionnaire, data were analyzed using logit model. Results indicated that factors such as home distance to the sea, type of fishing vessels, type of catch ownership, time and depth of fishing activity had significant effect in the incidence of illegal fishing. Fishing efforts of illegal fishermen for sturgeon harvest in Golestan province were mostly occurred during night and in shallow depths, and using non-standard fishing equipment. In conclusion, it appears that the probability of the occurrence of illegal fishing is in association with a range of social, economic, fisheries and conservation factors.

Excessive and illegal exploitation of aquatic resources, especially Caspian sturgeon, are serious threats to their long term protection. Given the importance of maintaining stocks of sturgeon, the study on major causes of committing illegal fishing by fishermen is inevitable. In this study, data were collected through field-based surveys and a questionnaire. Validity and reliability of the questionnaire were assessed based on expert’s knowledge and Cronbach's alpha coefficient. Sample size was estimated using Cochran's formula, and then 201 questionnaires (designed based on Likert scale) were distributed among fishermen in Guilan province during spring and summer 2016 to answer questions related to social, economic, fisheries and conservation factors. Data were analyzed using Logit Model. Results showed that social and economic factors such as study level, income and job satisfaction had significant correlations with the occurrence of illegal fishing (P

This study designed to evaluate blood parameters of 3-4 years Huso huso on pen in the center of the fishing and processing Ashuradeh (Chalasht) from the Gorgan Bay in different seasons.Blood samples from 10 Huso huso withaverage weight2698.01 ± 757.32 g and length 77.98 ± 5.53 cm were collected in each season (autumn 2014 to summer 2015) and activity of alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and variation in hematological parameters such as hemoglobin, erythrocyte count, leucocytes count, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in the blood samples of fish were measured in each season. There is significant difference in the enzymes levels were observed. (P<0.05). The lowest activity of aminotransferases and alanine aminotransferase were also observed in autumn. With the exception of mean corpuscular hemoglobin concentration (MCHC) that was the highest in the spring, the other blood parameters mentioned were highest in the autumn. The number of WBC (6.67 ± 0.35×103 mm3), RBC (0.97 ± 0.01×106 mm3) and hemoglobin values (6.96 ± 0.15 g/dl) had its lowest level in winter. But the levels of hematocrit (%21.80 ± 0.62), hemoglobin, MCV and MCH values respectively 187.50 ± 3.87 fL and 63.36 ± 1.45 pg/cell showed the lowest level in spring. The results of this study showed seasonal variations effect on haematological parameters in Huso huso and should be considered when these parameters are utilized to evaluate fish health status.

Salted roe was prepared from rainbow trout (Oncorhynchus mykiss) by adding food-grade pure and mixed NaCl (3.5, 4.5 and 5.5 %) to obtain optimal concentrations of both salts. Chemical, sensory and microbiological and color analysis were measured throughout the storage time to determine the changes that took place at 4°C and to evaluate the effects of the salt type up to 60 days. Total aerobic and psychrophilic microflora, mold and yeast were determined. Microbial population was lower in products with higher salt contents (4.5 and 5.5%). The decrease due to higher pure salt content of 4.5 to 5.5% was not significant. However, the addition of 4.5 and 5.5% pure and mixed salt at these levels did to inhibit total aerobic microflora growth at 4◦C and counts reached 5 to 6 logs CFU/g at 30 and 60 day, respectively. L* value of samples increased by increasing salt concentration of 3.5 to 5.5%. All of the salted roe significantly (p

The marine survey for sturgeon stock assessment was conducted to estimate the abundance of each species, total abundance and their percentage composition.In this study, a sample of 5 stations from the four fishing Golestan province which since 1380, the Iranian Fisheries as fisheries was done selected. Sampling was carried out using the gillnet. The sampling in 2011, 247 specimens including 105 A. persicus specimens, 14 A. guldenstaedti, 44 A. stellatus, 71 Huso huso and 13 A. nudiventris were caught. In 2012, of 281 specimens, 143 A. persicus, 8 A. guldenstaedti, 19 A. stellatus,19 Huso huso and 52 A. nudiventris were caught. Species composition in 2011, including 42 percent of A. persicus, 6 percent of A. guldenstaedti, 18 percent A. stellatus, 29 percent Huso huso and 5 percent A. nudiventris. In 2012, Species composition, including 59 percent of A. persicus, 3 percent A. guldenstaedti, 8 percent, A. stellatus, 22 percent Huso huso and 8 percent was A. nudiventris. The average length of A. persicus in 2011, 162.62±19.8 cm, A. guldenstaedti 149.45±16.26 cm, A. stellatus 126.4±17.42 cm, Huso huso 167.69±60.72 cm and A. nudiventris 139.06±12 cm. In 2012, the average length of A. persicus and A. guldenstaedti to the 144.55±42.12 and 145.8±13.92 cm. The average weight of A. persicus in 2011, 22.38±8.57 gr, A. guldenstaedti 19.24±6.55 gr and A. stellatus 8.70±2.76 gr. In 2012, the average weight of A. persicus 18.66±11.43 gr and A. guldenstaedti 20±7.08 gr. The catch per unit effort (CPUE) in 2011 sampeling, equivalent to 5.23 and sampling in 2013 was equivalent to the 5.73.

This study aimed to investigate the effects of salting of rainbow trout (Onchorhynchus mykiss) roe by pure and mixed salts (3.5, 4.5 and 5.5 %) on the quality and shelf life of the product, with respect to the contents of Total Volatile base nitrogen (TVBN), lipid oxidation (TBA), and on the proximate compositions during storage. The processing of roe leaded to changes in its chemical composition. In comparison with fresh roe, salted roe presented higher contents of TBA and lower content of TVBN. The greatest increases of TVBN were observed in the samples salted by salt without additives, in which TVBN was detected at 43.43 mg/100 g. pH value, salt absorption and TVBN and lipid oxidation had significant increase during storage (p

Nowadays, fish has been considered as one of the most important natural resources to provide nutritional requirements and support human health worldwide. On the other hand, seafood consumption has been reported as an important route of human exposure to a variety of chemical contaminants including heavy metals such as mercury. In this study total mercury levels were measured in 30 canned Yellowfin tuna (Thunnus albacares) from Famyla company product and then were compared with international standards. Weekly limit consumption based on the limit provided by the World Health Organization was provided. Wet digestion method was used and the samples were analyzed by ICP-OES. The results showed that the average concentration of mercury in this product was 0.126 μg/g of wet weight which was less than the allowable amount for human consumption determined by the international organizations such as WHO, FAO, USEPA, FDA, EC and MAFF. In this study, average available methyl mercury in canned Yellowfin tuna was 0.126 μg/g of wet weight which is less than the safety level of methyl mercury in fish. Risk index (HQ) was below 1 (0.54) therefore, the consumption of this product is not a serious threat to the consumer’s health and according to USEPA and JECFA standards, consumption of 2-3 meals of canned Yellowfin tuna per week is allowed.

The objective of this study was to introduce a simple method for identification of caviar species in caspian sea sturgeons. For this purpose, caviar samples of Acipenser gueldenstaedtii, A. percicus, A. stellatus, A. ndiventeri and Huso huso were collected from fishery stations, Khajenaphas, Torkaman and Chalasht in Golestan province and propagation center of sturgeons in Aghalla city. The extracted DNA from caviars were amplified using three primers termed R1, F2a and F1a, which were designed for cytb gene in the mtDNA. The primers of R1 and F1a were universlly introduced for all corresponding species in this study whereas R1 and F2a were spesific for A. stellatus. In addition, the results of sequencing obtained in cytb gene showed that the region studied here can be successfully utilized for identification of A. stellatus, A. ndiventeri and Huso huso species. phylogenetical study and the sequential corrolation among species studied verified the results obtained in this investigation. The results of this study can be perfectly used for identification of caviar in forensic,import and export issues.