عباس دل آذر

The genus Eremostachys (Lamiaceae), consists of about 60 species which 15 species of Eremostachys grow wildly in the North-West of Iran. The rhizomes and roots of E. laciniata have traditionally been used as a local analgesic and for the treatment of rheumatic diseases and pain in the feet. This experiment was conducted in the North-West of Iran in 2012-2013, to evaluate morphological diversity. The samples were taken from 15 regions. Thirty traits including plant height, inflorescence height, root length, root fresh and dry weight was measured. Simple correlation analysis showed the existence of significant correlations among root yield and tuberous root length (r=0.62), main root length (r=0.53), tuberous root number (r=0.57) at p≤0.05 level and tuberous root diameter (r=0.85) at p≤0.01 level. ANOVA showed that populations in 27 of 30 traits had significant differences. Factor analysis via PCA explained over 75.5% of the variation related to main effective characters. Populations of E. laciniata separated into four groups by cluster analysis using Ward method. Results showed that three populations i.e. Marand, Hyderabad and Till had high quantities in most of the traits and can be used in breeding and production programs.

Increasing the use of medicinal plants and limitation of water resources for production of these plants reveal the necessity of identification of the adapted genotypes to stress condition. Thus, two field experiments were conducted during 2009 - 2011 at the Research Farm of the Faculty of Agriculture, University of Tabriz, Iran, to evaluate response of Fennel Landraces to irrigation treatments. Experiments were arranged as split plot based on randomized complete block design in three replications with irrigation treatments (Irrigation after 60 mm, 90 mm, 120 mm and 150 mm evaporation from class A pan) in main plots and Landraces (Hamdan, Ezmir and Gaziantep) in subplots. Irrigation treatments had significant effects on all traits. Increasing irrigation intervals from 60 to 150 mm evaporation resulted in decreasing plant height, umbels per plant, seeds per umbel, seeds per umbelet, 1000-seed weight, plant biomass and grain yield. But umbelets per umbel was increased. Grain yield of Ezmir landrace was higher than that of Gaziantep and Hamdan landraces. It seems that due to the limited sources of water in the region, irrigation after 90 mm evaporation and Ezmir landrace are suitable for fennel grain production.

Two field experiments were conducted at the Research Farm of the Faculty of Agriculture، University of Tabriz، Iran during 2009-2010 and 2010-2011، to evaluate the response of fennel (Foeniculum vulgare L.) landraces to limited irrigation. Experiments were arranged as split plot based on randomized complete block design in three replications with irrigation treatments (Irrigation after 60، 90، 120 and 150 mm evaporation from class A pan) in main plots and landraces (Hamdan، Ezmir and Gaziantep) in subplots. Chlorophyll a، Chlorophyll b and Proline contents، soluble carbohydrates، relative water content and leaf water potential of fennel were recorded at flowering stage. The results showed that the all traits of fennel were significantly affected by limited irrigation. Chlorophyll a، chlorophyll b، relative water content and leaf water potential decreased by 30، 24، 27 and 41%، but proline and soluble carbohydrate increased by 41 and 23%، respectively، due to water deficit. Ezmir and Hamdan were more drought tolerant than Gaziantep land race. Therefore، fennel plant uses osmoregulation by increasing proline and soluble carbohydrate in order to tolerate water limitation.

The medicinal plant of Ornithogalum cuspidatum (Liliaceae) uses widely in Iranian traditional medicine for treatment of respiratory and inflammatory disease. Previous study on methanolic extract of Ornithogalum cuspidatum showed that it has cytotoxic effect. Therefore we aimed to study the cytotoxic effect of methanolic extract fractions of Ornithogalum cuspidatum on WEHI-164, fibrosarcoma cancer cell line. Fractions of methanolic extract of Ornithogalum Cuspidatum was prepared using SPE (Solid Phase Extraction).WEHI-164 cancer cell line were treated with different concentrations of 10%, 20%, 40%, 60%, 80% and 100% fractions of methanolic extract of Ornithogalum cuspidatum for 12 and 24 hours. Cytotoxicity and cell viability were measured by MTT assay. ELISA was performed to determine apoptosis after 24 hours in different concentrations of methanolic fractions. 10% and 20% fractions of methanolic extract had not cytotoxic effect but 40%, 60%, 80% and 100% fractions induced a significant growth inhibition in WEHI-164 cancer cell line with LC50 of 200, 96, 76 and 73 μg/ml respectively. Morphological changes of cell death in treated WEHI-164 cells were mediated via apoptosis. Apoptosis in WEHI-164 cells after 24 hours treatment with 200 μg/ml of 40%, 60%, 80% and 100% fractions were 36, 41, 42 and 49% respectively. The results of this study suggest that 80% and 100% fractions of methanolic extract of Ornithogalum cuspidatum contain higher cytotoxic and apoptotic compounds on WEHI-164 cancer cells and can be candidate for identifying and purifying of cytotoxic bioactive compounds.

IntroductionCervical cancer is the most common cancer in women living in developing countries. Recently, for treatment of diseases such as cancer, herbal medicine is used as a supplementary.The Aim of this study was assessment of anticancerous effects of polygonum aviculare herbal extract on Hela cervical cancer cell lineMaterials and MethodsHeLa cells were cultured in RPMI -1640 with 10% Fetal Bovine Serum in 5% Co2 and at 37ºC in different concentrations (0, 0.005, 0.05, 0.01, 0.025, 0.075, 0.1, 0.125, 0.15, 0.175, 0.2, 0.25, 0.3, 0.35, 0.5, 5 mg/ml) of polygonum aviculare. For assessment of viability of cells, trypan blue staining was performed. MTT assay was used for proliferation detection.

our results showed that in 0.15, 0.20 and 0.35 mg/ml proliferation of HeLa cells decreased according to MTT assay.ConclusionIt was proved that polygonum aviculare had antioxidant component and could be a scavenger of free radical. Because of high production of free radicals in diseases such as cancer, the use of the herbal medicine with high amount of antioxidant could be a supplementary treatment in cancer and other diseases.

Reliable findings indicate that inflammatory factors and glycosilated hemoglubin (HbA1c) disorders has the main role in pathogeneses of diabetes and its complications. Traditionally plant extracts have been used for diabetes control. This study was aimed to examine the effect of total and different fractions of black tea on inflammatory factors and glycosilated hemoglobin (HbA1c) in diabetic rats. This study was conducted on 56 rats which were randomly divided into 8 groups (7 rats in each group)and they were studied for one month. The weight of rats was 200 – 250 gr. Groups 1 and 2 were non-diabetic, groups 3–8 were diabetic, and diabetes was induced by intraperitoneal injection of streptozotocin 60mg/kg. Groups 1 to 8 were received by intraperitoneal injection: vehicle, total extrac (50mg/kgw), vehicle, total extrac (50mg/kgw)t, methanol fractions of 20%, 40%, 60%, 80% plus 100% respectively. In the end of study blood samples were taken from all groups and glycosilated hemoglobin (HbA1c) and inflammatory factors: Interleukin-1,6, TNFα and CRP were measured. In this study concluded that injection of total extract and 20% fraction of black tea had decreased inflammatory factors in diabetic and non diabetic rats (p<0.05). but did not on glycosilated hemoglobin (HbA1c). It can be concluded that injection of total extract and 20% fraction of black tea had positive effect on inflammatory factors in diabetic rats

Ornithogalum cuspidatum is one of the medicinal plants that has been used in Iranian traditional medicine as treatment for respiratory and inflammatory diseaes. Previous studies on other Ornithogalum species showed that thay have anti-cancer and cytotoxic activities. However, the exact mechanism has not yet been determind. In present study, the growth inhibitory and apoptotic effects of methanolic extract of Ornithogalum cuspidatum (MEOC) on WEHI-164 fibrosarcoma cell line, a type of soft tissue cancer, were evaluated. MTT assay was used for measuring the cytotoxicity and cell viability at 6, 12 and 24 hours and in different concentrations of MEOC. Also, ELISA was used to measure apoptosis after 12 hours in different concentrations of MEOC. The results showed that MECO had growth inhibitory and cytotoxic activities on WEHI-164 cell line, in three mentioned times. The MECO changed morphology of WEHI-164 cell lines in to apoptotic cells. Increasing extract concentration and time caused decreased in cell viability. MEOC caused induction of apoptosis in WEHI-164 cell line. In general, these effects depends on the concentration of MEOC (P< 0.01).

Curcuma longa possesses anti-inflammatory effects and inhibits growth of cancer cells. Principle compound of Curcuma longa is curcumin which could interferes with biological pathways. Telomerase is a ribonucleoprotein complex which prevents telomeres from shortening during cell division. In the most cancers, including breast cancer, expression and activity of this enzyme increases to compensate for chromosomes shortening and could be an attractive target of cancer therapy. Therefore, we aimed to study inhibitory effect of curcuma longa total extract on MCF-7 breast cancer cell line for evaluation of its possible anti-telomerase effect in these cells. MCF-7 cells were cultured, IC50 of extract was determined by MTT assay test and then, cells were treated with serial dilutions of extract. Finally, we used TRAP assay to determine inhibition level of telomerase in treated and control cells. IC50 was determined as 0.057mg/ml. Percentage of relative telomerase activity (expression) (%RTA) was decreased with increasing of Curcuma longa n-hexane extract. Considering inhibitory effect of Curcuma longa total extract on telomerase in the breast cancer cells, it is possible to use it for developing novel breast cancer therapeutics in the future.

Qualitative and quantitative analyses of ‘Zonouz’ and ‘Gala’ apples polyphenolic compounds were carried out by HPLC during the commercial harvest time. The results showed qualitative differences between the two apple cultivars regarding polyphenolic compounds. In general, ‘Gala’ had higher polyphenolic compounds compared to ‘Zonouz’. The highest polyphenolics content was recorded in the Gala's peel and seed. The predominant flavonoids in the Gala's peel were quercetin-3-D-galactoside and cyanidin-3-galactoside with 45.7% and 42.3% of the total identified components, respectively. Phloridzin dihydrate (86.3% of the total flavonoid compounds) contained the principal flavonoid compound in the Gala's seed. Cyanidin-3-galactoside and (-)-epicatechin were the main flavonoid compounds in the Zonouz's peel with 65.4 and 26.1% of the total flavonoids. Phloridzin dihydrate (82.6% of the total flavonoids) was the major flavonoid compound in the Zonouz's seeds. The Zonouz's Peel and pulp had higher flavonoid and phenolic acids content compared to Gala. Chlorogenic acid (1mg/g DW) and (-)-epicatechin (0.5mg/g DW) were the highest phenolic acid and flavonoid components in the Zonouz's peel and pulp, respectively. Rutin hydrate was another major flavonoid constituent of Zonouz's pulp with 5.8% of the total identified flavonoids. This compound was absent in the Gala's pulp. Caffeic acid content in the Zonouz's pulp (0.3 mg/g DW) was considerably higher than Gala (0.08 mg/g DW).

Quantitative and qualitative study of phenolic compounds (phenolic acids, flavanols and anthocyanins) in peel of Gala apple were implemented using analytical HPLC, fluorescence microscopy and epi-illumination light microscopy at three different growing stages [1- fruit early growing stage 2- mid-stage of fruit development (80 days after full bloom) 3- during commercial harvest time]. The results showed qualitative and quantitative differences between diverse growing stages regarding the content and accumulation of phenolic compounds. HPLC and microscopic studies showed that anthocyanin content of peel increased progressively during season. Microscopic data revealed that the highest anthocyanin accumulation occurred at the commercial harvest time. Our results showed that phenolic acids content decreased till mid-season but then, after their content had increasing pattern until commercial harvest time. Microscopic pictures verified this claim as well. Simultaneous comparison of HPLC and microscopy of derived data showed that flavanol content of peel increased towards mid season. In contrast, a drastic decline was recorded for flavanols during commercial harvest time. Comparative survey of microscopic and chromatographic data indicated that the great potential of fluorescence microscopy for study of phenolic compounds in apple peels. In brief, integrated study of phenolic compounds by means microscopic and chromatographic techniques is fast and easy methods for identification and quantification of phenolic compounds leading to accurate conclusion regarding elevation of apple fruit quality criteria.

Reliable findings indicate that lipid profile, oxidative stress and inflammatory factors disorders has the main role in pathogeneses of diabetes and its complications. Traditionally plant extracts have been used for diabetes control. This study was aimed to recognition of the most components in the most effective hydromethanolic fraction of Iranian black orthodox tea on lipid profile, oxidative stress and inflammatory factors in type I Diabetic rats. This study was conducted on 35 rats which were randomly divided into 5 groups (7 rats in each group). The weight of rats was 200 – 250 gr. Total of groups was diabetic, and diabetes was induced by intraperitoneal injection of streptozotocin 60mg/kg. Groups 1 to 5 were received: carrier, methanol fractions of 20%, 40%, 60%, 80% plus 100% respectively. In the end of study biochemical factors was measured. Data were analyzed by using one ways ANOVA, SPSS statistic methods. Analytical, Preparative and NMR were used to recognize of components in the most effective fraction. In this study concluded that the effective fraction on biochemical factors was 20%. The main component of 20% fraction were caffeine, epicatechingallat, quercetin and kaempferol. It can be concluded that injection of 20% fraction of black tea had positive effect on the prevention of incidence and develop of diabetes complications.

Ornithogalum cuspidatum Bertol is an Iranian species of the genus Ornithogalum L. (family: Liliaceae). In the Iran, the aerial parts of O. cuspidatum are used as food additives and also as an anti-irritant and relaxant by soothing the throat and bronchial tubes during dry coughs. Only limited phytochemical investigation has yet been carried out on O. caspidatum. The essential oils were obtained by hydrodistillation in a clevenger extractor and the composition of essential oils of flowers, leaves and bulbs were determined by coupled GC-MS analysis. The yields of essential oils of fresh flowers, leaves and bulbs were 0.016%, 0.007% and 0.037% respectively. The GC-MS analysis of the essential oils of bulbs, flower and leaves led to the identification and quantification of a total of 155, 20 and 23 compounds respectively. The major components of flower’s oil were hexacosane (31.49%), ethyl linoleolate (2.74%), tricosane (7.72%), tetracosane (3.46%), heptacosane (5.65%), Octacosane (25.22%) and tetracosane,11-decyl- (10.03%); and about leave’s oil were Hexahydro farnesyl acetone (5.71%), Palmitic acid (16.01%), tricosane (17.42%), linolenic acid methyl ester (9.01%), heneicosane (5.30%) and phytol (20.71%). Also the major components of bulb’s oil were nonane (2.65 %), 4-methyl nonane (2.76%), decane (9.65 %) and undecane (7.73 %). Analysis of the essential oil of different parts of O. cuspidatum showed that the essential oils of flower and bulb consisted mainly of saturated hydrocarbon compounds. Although, the essential oil of the leaves consisted mainly of from oxygenated hydrocarbons. Also, the essential oil of the flower contained oxygenated terpenoid compounds (4.84%).

The hypoglycemic effects of the Uritca Dioica (UD) extract, used for treatment of diabetes mellitus for many centuries, have been documented in several studies. The present study was designed to determine the possible mechanisms of hypoglycemic effects of UD on human muscle cells and RIN5F rat pancreatic b cells.

In the cell culture laboratory of the Drug Applied Research Center, pancreatic b cells and human muscle cells were prepared in multiple flasks containing culture media. Alcoholic extract of UD at concentrations of 50, 100 and 200 mg/mL were added to muscle cell flasks. The same concentrations of extract plus insulin were added to other muscle cell flasks. Glucose levels were measured in the flasks before and after 60, 120 and 180 minutes after adding of extract. Also the same concentrations of UD were added to flask containing RIN5F rat pancreatic b cells, and insulin and C-peptide level were measured at 0, 60, 120 and 180 minutes.

Mean glucose level in the muscle cell media with UD alone and UD plus insulin, at the concentrations and time intervals mentioned, did not change significantly. Insulin levels in pancreatic cells media, before and after applying of UD at different concentrations, and at different times was ≤0. 2 µg/ml. C-peptide (µg/ml) levels in these medias with a dose of 50 µg/ml of UD and at above mentioned times were 0. 31, 0. 33, 0. 86 and 0. 8 at concentration of 100 µg/mL they were 0. 7, 0. 2, 0. 4 and 0. 39, and at concentration of 200 µg/mL were 0. 32, 0. 33, 0. 93, 0. 77 respectively (Nonsignificant changes).

The results of the present study showed that alcoholic extract of UD was unable to increase insulin sensitivity in muscle cells and/or increase insulin and C-peptide secretion from RIN5F pancreatic b cells. It seems that hypoglycemic effects of UD were not mediated through the proposed mechanisms of this study.

Some kinds of epilepsy are refractory to treatment with standard antiepileptic drugs. Several plant species are used in the treatment of convulsions. In this study we attempted to extract and evaluate the protective effect Peganum harmala on convulsion induced by strychnine in mice. After collection of Peganum harmala seeds and ground to obtain powder. The methanolic extract was prepared. Albino Swiss male mice were orally pretreated dose dependently (15, 30 and 45mg/kg) with Peganum harmala (Methanolic extract), for a period of 15 days, also a group of animals received phenytion (30 mg/kg i.p). After eight hours the animals were injected intraperitoneally single dose of strychnine 1.7 μg/kg. The convulsion was observed on strychnine treated animals. The convulsion was significantly decreased among those animals pretreated with phenytoin (p< 0.05). However, the protective effect of, Peganum harmala was evident at the dose of 45 mg/kg and the starting of convulsion time along with its duration were delayed in comparison to control group, the respiratory arrest time were also delayed. Increased survival rate were observed on Peganum harmala/phenytoin treated animals. The results obtained from the present study indicate that the Peganum harmala at the doses of 15 and 30 mg/kg were not effective against strychnine induced convulsion. However, Peganum harmala was effective at the dose of 45 mg/kg and its effect was comparable with phenytoin. It seems that the Peganum harmala seeds inhibits convulsion in mice by modulating the glycine and NMDA receptors. However, the exact mechanism Peganum harmala seeds on convulsion induced by strychnine remains elusive and needs further experimentation.

The medicinal properties attributed to Crocus sativus L. (saffron) are extensive, but saffron taken in large amounts is highly toxic. This study was performed to elucidate the possible toxic effects of total extract of Crocus sativus L. stigma on liver, kidney and some hematological parameters in normal rats. Male Wistar rats were randomly assigned into four groups of eight animals each. Group 1 was treated with ISS as control and Groups 2 to 4 were treated with total saffron extract administered daily for 2 weeks intraperitoneally in doses of 0.35, 0.70 and 1.05 g/kg, respectively. Body weight of the animals were recorded on the first, 7 and 14 of the experiment Hematological and biochemical parameters were measured on final days of the experiment. Tissue specimens of the liver and kidneys were subjected to histological examination using standard hematoxyline eosin staining. Total saffron extract caused significant reductions in the Hb and HCT levels and total RBC count, although it showed no dose-dependent effect. Total WBC count showed significant dose dependent increases in extract treated rats. The extract treated animals, also, exhibited significant dose-dependent increased values of AST, ALT, urea, uric acid and creatinine. In the histopathological studies of liver and kidneys in extract treated rats, there were mild to severe tissue injuries supporting the biochemical analysis. The results of this study indicated that total extract of Crocus sativus L. stigma is toxic (in given doses), and causes hepatic and renal tissue damages along with anemia in rats.

Ribes biebersteinii fruit is used for treatment of hypertension in folk medicine of Azarbaijan. However it's mechanism of action is not studied. This work was aimed to examine the vasorelaxant effect of the Ribes biebersteinii fruit total extract (RBE) and role of endothelium in rat isolated aorta. Rings of aorta (3-5 mm length) were prepared and equilibrated in Kreb's solution under 2 g tension. Rings with or without endothelium were contracted by phenylephrine (PHE, 0.1μM) or PGF2α (8 μM) and then exposed to cumulative doses of RBE. In order to asses the role of endothelium in the vasorelaxant effect of RBE, tissues were studied by incubation for 20 min by (L-NAME, 100 μM) or (Methylene blue, 10μM) before contraction by PHE. RBE induced relaxation in rat aortic rings pre-contracted with PHE or PGF2α dose–dependently in both intact and endothelium-denuded aortic rings. The differences between the relaxant response of intact and endothelium denuded rings were not significant (p>0.05). L-NAME and Methylene blue did not affect the RBE-induced relaxant response in rat aortic rings. the results indicate that RBE induces relaxation dose-dependently in rat aortic rings precontracted with PHE by a mechanism independent of endothelium function or its productions.

Because of eventual side effects of chemical drugs, the efficacy of natural wound healing accelerators in long-term diseases and some situations is demanded to practitioners. The initial aim of our study was to assess full thickness excisional skin wound healing and inflammation diminution, Morphometrically and Histopathologically, after topical application of dried extract of Echinacea purpurea aerial part in rats, compared with zinc oxide. Sixty wistar rats received four full thickness excisional wounds with the aim of surgical punch on the back skin under surgical anesthesia.

Nowadays, the promotion of wound healing in some diseases and chronic disorders with the aid of herbal extracts is more challenging than ever before. That is why new compounds prepared for this purpose have been widely accepted. Echinacea purpurea has shown anti-inflammatory, immune stimulatory, fibroblastocyte stimulatory as well as anti-hyaluronidase and anti-microbial effects; therefore, it must hasten wound healing. The aim of this work was to evaluate histometrically and histopathologically the ability of dried extract ointment of Echinacea purpurea in enhancing the healing process and in reducing phlogosis of full thickness excisional skin wound in rats following topical application, compared with zinc oxide ointment by the same vehicle of eucerin. Under surgical anaesthesia, four full-thickness similar excisional wounds were made on the back of 70 rats by punching that divided into 5 groups of Echinacea purpurea 20% and 10% ointment, zinc oxide ointment, eucerin, and control. A double-blind method was employed throughout the study. All the cases were treated with topical ointments daily for 21 days. Healing process of the wounds were daily quantified and compared using digital photography and image analysis software. Histopathologic examination was performed in the 0th, 3rd, 7th, 14th, and 21st days and the wound healing was scored using healing grades I to VI regard to the wound healing parameters such as oedema & phlogistic process, congestion & haemorrhage, fibroplasia, epithelium regeneration, wound contraction, Collagen deposition and granulation tissue maturation. The overall outcome of the wound recovery for each individual group was calculated, and the results were put under a series of statistical analysis through SPSS. According to the histometric findings, the skin contraction rate in Echinacea purpurea 10% group (group II) on treatment period was much higher than that in the other groups. Also, histopathologic results revealed that overall healing rate in the group II in the second and third weeks was higher than that in the other groups (P < 0.01). Echinacea purpurea could be a new promising therapeutic approach to improve skin wound healing because of its potential anti-inflammatory and wound healing stimulatory effects.

Nowadays, nondrug treatments (mineral waters and plants) are novel therapeutic approaches for hyperlipidemia. The aim of the present study was the evaluation of the effects of calcic and magnesic sulfate mineral water and total extract of Hypericum perforatum on the serum lipid profile (T-CH, TG, HDL-CH, LDL-CH) in hypercholesterolemic rats. Thirty male rats (SD) weighing 100-150g were randomly divided into 5 experimental groups. The rats in control group were fed a standard diet and the other groups were fed a cholesterolrich diet for 60 days. Then, hypercholesterolemia was observed through analyzing the blood samples (P<0.001). In the second phase(therapeutic phase), control group(group1) was continued with standard laboratory diet, and hyperlipidemic rats were treated with total extract of Hypericum perforatum, tap water or mineral water for the next 60 days, as the following: group2: cholesterol-rich diet and tap water, group3: cholesterol-rich diet, tap water, total extract of Hypericum perforatum (4mg/kg, ip), group4: cholesterol-rich diet, tap water and total extract of Hypericum perforatum (12mg/kg, ip), group5: cholesterol-rich diet and mineral water. All groups received saline normal and DMSO as a solvent for extract through intra peritoneal (ip) injection. At the end of the period, blood samples of each group were drawn from venus plexus of eye and analyzed for the lipid levels of plasma. The results indicated that treatment by mineral water or total extract of Hypericum\ perforatum at the doses of 4 and 12mg/kg could reduce the levels of T-CH, TG, LDL-CH significantly (P<0.001). Total extract of Hypericum perforatum (4mg/kg, ip) and mineral water administration alter a statistically significant difference to the level of TG (P<0.001), but could not reduce T-CH, LDL-CH levels. Calcic and Magnesic Sulfate mineral water and total extract of Hypericum perforatum could reduce lipid profile especially TG. More studies are recommended.

Satureja (F: Labiatea) species are present in the worldwide. 12 Satureja species grow in Iran that 8 species are endemic. These plants are famous due to use as a culinary and medicinal plants. Methodes: In this research the aerial parts of Satureja sahendica were collected from east Azarbayejan provinence, Osku at E: 46 10 3.6, N: 37 43 52.7 and 2048 m heigh. The air dried parts of plants were kept at 2ºc up to used for GC analysis.The plant materials seprated to two different parts, inflorescence (S1), Leaf and stem(S2). 30 – 40 gr of each samples were subjected to hydro – distillation for 4 h using a clevenger – type apparatus to produce essential oil. Hydrodistillation was repeated thrice for each sample and average was calculated. The essential oil was found to be yellow liquid and was obtained in yields of 1.66 ± 0.19 for S1 and 1.5 ± 0.12 for S2 based on v/w. 28 compounds were identified in the oil of S1 that thymol (32.5 %), γ– terpinen (29.33 %) and p– cymene (23.48 %) are main constituents and 23 compounds were characterized in the oil of S2 that main constituents were p– cymene (44.88 %), thymol (28.22%) and γ– terpinen (10.07 %). The antioxidant activitities of S1 and S2 samples were assessed by DPPH assay and RC50 values were 7.38×10−2 and 7.15×10−2mg/ml, respectively. Investigations showed that the essential oil of aerial parts of S. sahendica had a high antioxidant activity and due to high amount of thymol and Pcymene and other terpenoids in the oil, it can be used as a culinary and medicinal plant and perfume industries.