فرشته مرشدی

Sertraline is an antidepressant drug and many controversial reports have been presented on the complications of sertraline on the reproductive system. In this study we evaluated the protective effects of vitamin E against sertraline-induced damage to the reproductive system of male mice.

In this experimental study, 40 adult male mice were divided into 8 groups of 5. Four groups received 100 IU/kg.bw vitamin E by gavage for 42 days. One group was considered as control and three groups received just sertraline with doses of 5, 10 and 20 mg/kg.bw orally. 24 hours after the last treatment, testes tissue and blood samples were collected and sent for histochemical and biochemical studies, spermatogenic indices as well as measurement of the expressions of Bcl-2, Caspase-3 and Hsp70-2 genes.

The results showed a significant increase in the level of malondialdehyde, nitric oxide and expression of Caspase-3 and Hsp70-2 genes. We also found a significant decrease in spermatogenesis indices, total antioxidant capacity, testosterone and Bcl-2 gene expression in the group receiving 20 mg/kg.bw sertraline (P<0.05). The above mentioned parameters were improved in the groups that received vitamin E along with sertraline.

Vitamin E can decrease the adverse effects of sertraline on biochemical and histochemical parameters, spermatogenic indices and can increase the expression of Bcl-2, Caspase-3 and Hsp70-2 genes in the testicular tissue of mice.

Sertraline is one of the antidepressants that little information about the side effects of this medication on the adrenal glands is available. The present study was conducted in order to evaluate the protective effects of vitamin E against sertraline-induced injuries on serum and tissue structure of adrenal glands in mice. 40 adult male mice were randomly divided into eight groups, each five. Four groups of mice received vitamin E at the dose of 100 IU/kg body weight orally for 42 days. Three groups of these, following the vitamin E administration, received sertraline at 5, 10 and 20 mg/kg body weight, respectively. The remaining three groups, received sertraline at 5, 10 and 20 mg/kg body weight. And one group was considered as the control. 24 hours after the last treatment, blood samples were collected to measure MDA and TAC levels from the heart. The adrenal glands were removed from the abdominal cavity and histological and histomorphometric changes were examined by light microscope and digital camera. Specific staining methods including toluidine blue for identifying mast cells, periodic acid-Schif to determine carbohydrates and masson's trichrome for determining fibrosis in adrenal tissue were performed. Sertraline at 20 mg/kg, significantly decreased in TAC level and significantly increased the sponge cell diameter, MDA level and thickness of fasciculata and reticularis zones compared to the control group. Although, no significant changes in the thickness of capsule and glomerulosa zone were observed. Specific staining showed that sertraline induced an increase in the fibrosis tissue, but did not change significantly the amount of carbohydrates between the groups. The administration of vitamin E markedly improved the changes observed in these parameters. It could be deduced that Vitamin E reduces the toxicity of sertraline in mice adrenal glands by improving tissue and serum indices.

Sertraline is one of the most widely used antidepressants in the world. The present study was performed to evaluate the protective effects of vitamin E against sertraline-induced damage on testicular tissue in adult NMRI mice.

In this experimental study, 40 adult male NMRI mice were divided into eight groups of five. The first control group, received 0.3 ml of normal saline per day by gavage. The second, third and fourth groups received sertraline at 5, 10 and 20 mg/kg body weight per day by gavage,respectively. The fifth group received vitamin E at 100 international units per kg body weight per day by gavage. The sixth, seventh, and eighth groups received sertraline at 5, 10, and 20 mg/kg body weight, along with vitamin E at 100 international units per kg body weight per day by gavage, respectively. After 42 days of treatment, testicular specimens were collected and used for histomorphological, histomorphometrical and histochemical studies. The obtained data were analyzed in SPSS v. 19 using one-way Analysis of Variance (ANOVA) and Tukey’s test at the significance level of (p<0.05).

Sertraline caused destructive histomorphological and histochemical changes in testicular tissue and also significantly decreased the diameter of the seminiferous tubules, the height of the germinal epithelium, the number of spermatocytes, the number of Leydig and Sertoli cells and the Johnsen’s score compared to the control group (p<0.05). Vitamin E administration improved the histomorphological, histochemical condition and significantly increased the histomorphometric parameters in testicular tissue (p<0.05).

Vitamin E can improve the adverse effects on histomorphological, histomorphometrical and histochemical parameters in the testicular tissue of mice receiving sertraline.

Sertraline is one of the antidepressants whose effects on the reproductive system has faced controversial reports. This study was performed to evaluate the efficacy of vitamin E against sertraline-induced injuries on serum and sperm parameters.

In this experimental study, 40 adult male mice were divided into eight groups of 5. Four groups of mice received vitamin E at 100 international units per kg body weight via gavage for 42 days. In three of the above groups, sertraline was administered orally at 5, 10 and 20 mg/kg body weight four hours after receiving vitamin E. One group was considered as the control group. Twenty-four hours after the final treatment, blood samples were collected from the heart and sperm quality parameters including count, motility, viability, chromatin density, abnormal forms, and sperm DNA damage were measured.

Sertraline significantly increased abnormal sperm (25.8±2.04) and significantly decreased the count (30.2±2.77), motility (68.30±2.94), viability (75.4±3.20) and maturation (84.0±1.58) of sperm compared to control group (10.4±1.14, 39.6±2.07, 89.37±1.87, 92.6±3.71, and 97.4±1.14, respectively) (P<0.05). Vitamin E administration significantly improved abnormal sperm (17.8±2.28), count (37.2±1.92), motility (78.27±2.10), viability (83.2±2.38) and maturation (83.4±1.81) of sperm (p<0.05).

The results of this study showed that vitamin E decreases the reproductive toxicity of sertraline in mice by improving serum and sperm parameters.

Sertraline, along with its beneficial antidepressant effects, may have adverse effects on the fertility and reproductive system. In recent years, the negative effects of sertraline on body tissues have been shown to some extent. This study aimed to investigate the effects of sertraline at different doses on the histological and histometrical structure of ventral and anterior lobes of prostate in adult mice.

In this experimental study, twenty adult male mice were randomly divided into four groups: control (no treatment) and three groups receiving sertraline at doses of 5, 10 and 20 mg/kg by gavage for 42 days. First, the mice were sacrificed, then, blood samples and prostate lobes tissue were collected for histomorphological and histochemical studies.

Results showed a significant decrease in cell count of secretory units in ventral and anterior lobes in the sertraline groups, compared to that in control group (p<0.05). Epithelial thickness showed a significant decrease in ventral and anterior lobes of prostate following the administration of sertraline (20 mg/kg), compared to the control group (p<0.05). A significant dose-dependent decrease was seen in serum testosterone in sertraline groups, compared to the control group (p<0.05).

Sertraline has a negative effect on the histopathological and histometrical structure of prostate. However, further clinical studies are required to assess the negative effects of sertraline in human beings.