فهرست مطالب مجید رضا خوش خلق

The aim of the present study was to evaluate effects of different levels of thyme, Zataria multiflora (n growth performance, body chemical composition and resistance to environmental stressors such as temperature and acidity in zebra fish, Danio rerio. 480 Fish with an average weight of 0.33 ± 0.08 g were distributed in 12 tanks and fed by four isonitrogenous and isoenergetic diets including replacement levels of 0, 1, 1.5 and 2% thyme powder (T0, T1%, T1.5% and T2% respectively). After 10 weeks, growth performance, survival and body proximate composition, were determined, then 10 fish per tank were taken for environmental resistance and 10 fish per tank for the body composition analysis. According to the results, the highest final weight, weight gain, body weight increase, specific growth rate and protein efficiency ratio were obtained in T2% (p < 0.05), while the lowest feed conversion ratio in T2%. It was also true for the highest survival rates which were also found in T2% but not significant (p>0.05). Differences among treatments in survival rate against acidity and temperatures were also not significant (p>0.05). Significant difference between treatments were observed in body composition including moisture, protein, fat and ash (p<0.05). In conclusion, using thyme powder at the food dietary of the zebra fish, can affect the growth performance, carcass composition and survival rate of D. rerio but not on resistance against the environmental stressors.

This study was conducted to evaluate the effects of replacing fishmeal with fermented soybean meal on some hematological and immunological indices of Beluga, Huso huso. For this purpose, 240 Beluga (394.6 ± 4.3 g) were distributed in eight treatments (with three replications). The experimental treatments included T1: fish meal (FM), T2: (FM + methionine and lysine), T3: Fermented soybean 40% (FSBM40), T4: (FSBM60), T5: (FSBM80), T6: (FSBM40 + methionine and lysine), T7: (FSBM60 + methionine and lysine) and T8: (FSBM80 + methionine and lysine). During the 56-day feeding period, the fish were fed to apparent satiation three times daily. The results showed that there was no significant difference in hemoglobin, hematocrit, MCHC, neutrophil, monocyte and eosinophil values (p>0.05). However, the highest number of white and red blood cells was observed in the diets containing FM and FM + methionine and lysine, respectively (p<0.05). Furthermore, higher amounts of MCV and lymphocytes in T6 and higher amounts of MCH in T5, T6 and T8 were observed than in the other treatments (p<0.05). Also, the highest amounts of lysozyme were observed in T4. In addition, T4 and T7 exhibited higher amounts of IgM and total immunoglobulin than the other treatments (p<0.05). Based on the results, the best performance of hematological and immunological indices was observed in replacing 40-60% fishmeal with fermented soybean meal.

In this study, the possibility of using migratory locust as a source of protein in the diet of rainbow trout fry and its effect on growth performance, immunity and hematology of blood serum was investigated. 300 number of rainbow trout fingerling with an average weight (6.09 ± 0.22 gr) with diets of 0% (control), 15% (LM15), 30% (LM30) and 60% (LM60) of formulated locust meal. became Each diet was distributed in a completely random design in 3 replicates with 30 number of fish in each fiberglass tank with the same conditions. At the end of the experiment, the indicators of growth, immunity and blood serum hematology were calculated based on standard formulas and analyzed using Tukey's test. The results showed that the highest final weight was observed in the 30% treatment (616.20% ± 34.89%) and the difference between zero, 15% and 60% treatments was significant (p<0.05). In the 60% treatment, a significant increase in feed conversion ratio was observed compared to the control treatment (p < 0.05). In feeding fish with a diet containing locust meal, the values of glucose, albumin, globin and IgM did not show significant differences among different experimental treatments (p<0.05). However, the amount of total protein, triglyceride and blood cholesterol showed a significant difference in the experimental treatments compared to the control treatment (p < 0.05). Therefore, it can be concluded that the use of 30% of locust meal in the commercial diet of rainbow trout fingerling can improve the growth performance and have no negative effect on the desired level of blood and immune parameters..

This study aimed to investigate the effects of casein blood substitute feed on the growth, survival and reproduction of Hirudo orientalis. At first, 100 leeches with an average weight of 3 g were reproduced. The growth indices of 200 leeches (with an initial weight of 0.038 ± 0.012 g) were measured during 10 months and also reproductive characteristics of 50 leeches during the 8th to 10th months. Leeches were fed at 30-day intervals under two treatments: using fresh blood (T1) or blood substitute feed (T2). During the rearing period, the growth of blood-fed leeches (T1) was significantly (p<0.05) faster than the blood substitutes-fed group (T2). In the 10th month, the two groups exhibited an 18% weight difference with each other. Although the amount of weight, length, diameter and number of cocoons produced in T1 were higher than T2, however the differences were not statistically significant (p<0.05). Survival rate was higher in T1 during growth and reproduction. Fertility was diagnosed in almost all adults in T1 (98%) and in most leeches of T2 (91%). The number of infants in T1 was significantly (p<0.05) higher than T2. It can be concluded that casein blood substitute feed causes less growth, reproduction and survival rate compared to fresh blood (p<0.05) in H. orientalis. However, due to health and breeding problems, using fresh blood in the reproduction and breeding time intervals of the leeches on the one hand, and the proximity of the results of the two treatments on the other hand, it can be concluded that the blood substitute feed reduces production costs, facilitates feeding of leeches and producing hygienic leeches, hence.

In this study, the possibility of using locusts as a source of protein in the diet of rainbow trout and its effect on growth performance and blood biochemical parameters were investigated. 300 fish with average weight (5.08 ± 0.33g) with diets of 0% (control), 15% (SG15), 30% (SG30) and 60% (SG60) locust meal were formulated, with equal protein energy contents. Diet was distributed in a completely randomized design in three replications for eight weeks and twenty-five pieces of fish with the same conditions, in each tank. At the end of the experiment, growth, biochemical and safety indices were calculated based on standard formulas and the analysis was performed using Tukey test. The results showed that the highest final weight was observed in 30% treatment )89.34 ± 20.61%) and the difference between 0, 15 and 60% treatments was significant (p <0.05). In 60% treatment, a significant increase in feed conversion ratio was observed compared to the control treatment (p <0.05). In feeding fish with locust meal diet, glucose, cortisol, albumin, globin and IgM levels did not show significant differences between different experimental treatments (p <0.05). However, the amount of total protein, triglyceride and blood cholesterol showed a significant difference in experimental treatments compared to the control treatment (p <0.05). Therefore, it can be concluded that the use of 30% locust meal in the commercial diet of rainbow trout fingerling, in addition to adversely affecting blood and safety parameters, can improve growth performance.

The present study was conducted to evaluate of different levels of locust meal (Schistocerca gregaria) on growth function, survival, biochemical composition and intestinal digestive enzymes in rainbow trout fingerling. Four experimental diets with different replacement levels of 0 (control treatment), 15 (SG15), 30 (SG30) and 60% (SG60) of fish meal with locust meal were formulated with equal protein (46.5%) energy (4652 kcal) contents. Diets were distributed in a completely randomized design in four treatment and each with three replications and the average initial weight (5.08±0.33) in 12 tanks for eight weeks (25 pieces of fish in each tank) and Feeding was done three times a day to satiety. Physicochemical parameters such as temperature (17.3°C), dissolved oxygen (6.49 mg), pH (7.55) were constant during the experiment. The results revealed that the highest final weight was observed in 30% treatment (89.34±20.616 %) and differences between 0, 15 and 60% treatments (p<0.05). In 60% treatment, a significant decrease in growth indices and nutritional efficiency was observed (p<0.05). Also, the amounts of protein, fat and moisture in the body of fish were significantly different compared to the control treatment (p>0.05). Analysis of digestive enzymes showed that the highest activity of protease and amylase enzymes was observed in fish fed SG30 diet. The results of this study showed that using 30% locust meal instead of fish meal can improve growth performance, the biochemical composition and digestive enzymes rainbow trout fingerlings.

Genetic structure of Acipenser persicus, in the Caspian Sea was studied using tetrasomic microsatellite markers. A total of 195 specimens of A. persicus breeders were collected from the sampling stations located in the five fishery catch zones as well as from the Sefidrud River in the south Caspian region. About 2 g of caudal fin samples was collected from each sturgeon specimen and preserved in 96% ethyl alcohol and then transferred to the genetic laboratory of the International sturgeon research Institute. Genomic DNA was extracted using the phenol-chloroform method. DNA quality and content was determined using spectrophotometry and agarose gel electrophoresis. Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 8 pairs of microsatellite tetrasomic primers of which all of primers were polymorphic. All PCR products were electrophoresed on 6% polyacrylamide gel and stained with silver nitrate. Following the scoring of alleles, all parameters related to population genetics were calculated using the Phylip and Arliquin program and the phylogenetic relationship between samples was plotted using neighbor-joining tree. Number of alleles in A. persicus varied from a minimum of 8 (Zone 2) to a maximum of 14 alleles per locus (Sepidrud River). The observed and expected heterozygosity was between 0. 657 and 0. 784 respectively. Significant differences were detected between A. persicus specimens collected from Sepidrud River (P≤0. 001) and those collected in the south Caspian Sea. Significant differences were also detected between specimens caught in the eastern fishery zones and those caught in the western fishery zones of the south Caspian Sea (P≤0. 001).  Based on genetic criteria it is evident from the phylogenetic tree plotted that A. persicus specimens collected from the Sepidrud River belonged to one cluster and all other specimens belonged to a separate cluster. Based on the results obtained it may be concluded that three independent populations of A. persicus are found in the Caspian Sea which include the Sepidrud population, and the population of the fishery zone three in which this calls for further investigations on the genetic structure.

The genetic diversity and population structure of cultured male and female herds of beluga sturgeon (Huso huso) brood stocks were estimated in International Sturgeon Research Institute using four DNA microsatellite loci. All loci were in polymorphic pattern. The number of alleles and observed heterozygosity ranged between 6-37 (mean 18) and 0-0.87 (mean 0.41), respectively. Allelic richness (Na) was acceptable and genetic variation was in moderate decreasing pattern.  For survey of genetic differentiation in individuals, the Fst was estimated at 0.045, indicating a low genetic differentiation among breeders. Samples from male herds in Spl-104 and Ls-19 loci were at Hardy-Weinberg equation and other loci showed a pattern of deviation from equilibrium, which could be as a result of reduced population size and the presence of an inbreeding phenomenon. According to the results obtained from molecular variance analysis, the genetic difference between two populations was 3%. The results of the study indicate that genetic diversity was low in the cultured beluga brood stocks, resulting in the reduction of wild beluga breeders in the southern basin of the Caspian Sea.

Direct mitochondrial DNA (mtDNA) control region sequencing analysis was used to investigate population genetic structure of Persian sturgeon (Acipenser persicus) in the Caspian Sea. A total of 45 specimens were collected from different locations of the Caspian Sea. mtDNA control region was amplified using PCR. Direct sequencing was performed according to a standard method. The results showed that 12 haplotypes were observed among 45 samples in the method. The highest numbers of haplotypes were observed in the Sefidrud River in which 3 haplotypes A, B and E among them were specific for the river and were not observed in the other locations. The average haplotype diversity (h) and nucleotide diversity (π) were 0.795±0.037 and 0.0062±0.0046, for control region sequencing, respectively. The results of Fst based on kimura- 2 parameters method and analysis of molecular variance (AMOVA) demonstrated that most variations occurred among the samples, and between samples of the Sefidrud and Russia and Azerbaijan are statistically significant (P<0.0001). Therefore, three distinct populations including Sefidrud, Russia and Azerbaijan were identified. As mtDNA control region is a highly variable segment, it may be used as a potential marker for identifying populations and for determining their management and conservation units, leading to the useful application of molecular markers in investigating conservation genetics of the Persian sturgeon.

The aim of this study was to determine the use of olive pomace in the diet and effect on growth performance and blood parameters in goldfish (Carassius auratus). Fish with average weight of 13.73 ± 0.03 g were fed for 8 weeks with five diets including controll diets without olive pomace and diets containing 2, 4, 6 and 8% dry and alcoholated olive oil. Finally, blood samples were taken from caudal vein and hematological indices including red (RBC) and white blood cells (WBC), differential count of WBC, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration were determined. Based on the results, fish were fed by the diet supplemented with 2 percent olive pomace displayed significant difference in final weight, weight gain, specific growth rate, body weight increase, condition factor, protein efficiency ratio and lipid efficiency ratio (P<0.05), while dietary olive pomac had no effects on the final length and feed conversion ratio of goldfish fed different diets (P>0.05). Differences among treatments in all hematological indices were not significant (P>0.05). According to the results of this study, the use of olive pomace in goldfish diet up to 2% is suggested.

The aim of the present study was to investigate the effects of olive pomace replacement on blood indices and growth of common carp of Cyprinus carpio Common carp with average weight of 19.97 ± 0.05 gr. And average length of 10.55 ± 0.49 centimeters with 6 diets including 0, 3, 6, 9, 12 and 15% olive pomace for 8 weeks in The aquarium was fed. Feeding three times a day at satiation fish was done. After 56 days, the results showed that the 6% level showed the highest growth in terms of weight, length, weight gain, body weight gain, specific growth rate, feed efficiency and liver index But the difference in viscera index was not significant.Blood parameters of common carp showed that in some indices, including RBC, hemoglobin, hematocrit, monocyte and MCHC, no significant differences were observed But in the number of white blood cells, neutrophils, lymphocytes, MCH, MCV, there was a significant difference. The results of this study indicate positive effect of olive pomace replacement to 6% level without negative effect on growth performance and blood factors in common carp ruminant diet. And you can suggest a level of 6% as the appropriate level for replacement.

The aim of this study was to investigate the primary and secondary responses of Siberian sturgeon (Acipenser baerii) to handling stress on at two different sizes. In this study, the fish were distributed in small and large sizes with mean weights of 672.27 ± 2.54 g and 1078.5 ± 5.30 g, respectively, in six replicates with equal biomasses. Six blood samples were randomly taken before the stress thereafter three blood samples were taken at 1, 3, 6, 12 and 24 hours after acute stress (the fish were exposed to air for two minutes) to examine the physiological changes of blood. Cortisol levels increased in both sizes, although there was no significant difference in experimental treatments. According to the results, blood glucose increased in large size after stress and a significant difference was observed (p 0.05), but no significant was observed in small size (p 0.05). Lactate levels significantly increased in both sizes after stress (p 0.05). The results showed that large-sized fish show larger stress response than small size, and therefore they tend to be more susceptible to stress.

Understanding the reproductive regulation in sturgeon is a precondition for controlled reproduction. Although 11-ketotestosterone (11-KT) has been found in blood of female in several fish species, the importance of this androgen in great sturgeon female has not been investigated. To address this issue, the effect of 11-KT on previtellogenic Huso huso was examined for the first time. A total number of twelve fish (mean weight 5,580±165 g) were divided into two circular tanks and reared under natural conditions (natural river water with a natural temperature profile). The fish were surgically implanted with slow-release pellets containing only a carrier matrix (95% cholesterol, 5% cellulose) without or with 2.5 mg 11-KT. Changes in the plasma levels of hematological and biochemical parameters were evaluated three weeks and two months following implantation. The results showed that red blood cells and its related parameters and white blood cells were affected significantly (P

This study investigated effects of different levels of dietary olive oil pomace on body composition, growth performance, hematological factors and sensory analysis of rainbow trout. Six practical diet containing 0 (control), 2, 4, 6, 8 and 10% olive pomace were formulated and supplied to fish with an average body weight of 184.7 g being allocated randomly to rectangular concrete ponds. Water flow was 10 L S-1. Temperature and pH were 10±3°C and 6.8-7.9, respectively. Fish were fed by hand ad libitum three times a day. At the end of the experiment, eviscerates and beheaded body composition and growth performance were not affected by difference levels of olive pomace (P > 0.05). However, hematological parameters were significantly affected by increasing olive oil pomace (P

Distribution and abundance of order Ephemeroptera, an important insect order in running waters was examined in Shafaroud River (a river entering the Caspian Sea). For this purpose following preliminary visit to the river some 6 sampling stations were selected from upstream to the estuary of the river. Monthly sampling was carried out from July 2013 to June 2014. A Surber sampler with mesh size of 500µ and area of 0.16 m2 were used for sampling. The collected samples were isolated from debris and sediments, immediately preserved in 4% formalin solution and transferred to the laborator for identification and enumeration. Some physical such as (air and temperature )and chemical parameters of the water such as dissolved oxygen(DO), and physicochemical parameters of the water were including electron conductivity (EC)Acidity( pH) and salinity, were measured simultaneously with benthos sampling at all sampling sites. In total 8100 specimens belonging to 5 families and 9 genera of Ephemeroptera were collected and identified out of which Heptagenidae and Baetidae were the dominant families and Baetis and Rhithrogena dominant genera respectively. In most seasons Significant statistical difference were observed between upstream and downstream sampling stations (p ≤ 0.05).

In this study population genetic structure of freshwater crayfish (Astacus leptodactylus) in Iran was investigated using direct sequencing of mtDNA control region. A total of 132 samples were collected from the different locations. The quality and quantity of total DNA were determined by agarose gel electrophoresis ethidium bromide staining and spectrophotometery, respectively. The results showed that 38 haplotypes were observed between samples in sequencing analyses. The haplotype diversity (h) and nucleotide diversity (π) were 0.811±0.049 and 0.0127±0.0038 sequencing techniques, respectively. Tajima’s D and chi-square (χ2) values were 3.120 and 78.25, respectively. The significant positive D value for the samples might suggest balancing selection. Significantly negative Fu's Fs values and significantly positive Harpending test values were taken as evidence of a population expansion.The results of FST ,Exact test and analysis of molecular variance (AMOVA) demonstrated that samples between Siahdarvishan River , Jafrood River and Astara region in the southwest Caspian sea statistically are significant in sequencing techniques (P

The present study was designed to investigate the effect of bacteria Weissella cibaria as to probiotic on growth factors on Siberian sturgeon. The fish averaged 143.55 ± 0.04 g in weight were placed in 12 tanks for 60 days in four treatments everyone to three replicates including 0.2 g probiotic containing 107CFU / g for the first treatment (TA); 2 g probiotic containing 108CFU / g for the second treatment (TB) and 20 mg probiotic containing 109CFU / g for the third treatment (TC), and diet without probiotics for the control treatment (C), respectively and at the end of the trial meantime measurement the growth rate proceed to means Statistical analysis (ANOVA one- way- 95%) although, all probiotics-treated diets were resulted in to increases parameters feed conversation ration (FCR), specific growth rate (SGR) and protein efficiency ratio (PER), body weight index (BWI) comparing to the control group. However, no significant difference Condition Factor (CF) was observed between the treatments and group. Thus using this type of probiotics can be effective in improving the growth parameters

This study was carried out to evaluate the effects of different dietary protein (30, 35, 40%) and lipid levels (10, 12, 14%) on growth performance and physiological changes of juveniles Caspian Kutum (Rutilus frisii kutum). Six hundred and seventy five fish (1.15 ± 0.01 g) were distributed into 27 aquaria (45 L) and were fed four times daily for 8 weeks. Interaction between protein and lipid did not significantly affect the growth factors (p > 0.05). Results showed that increasing the level of protein to 35% significantly increase some growth indices such as final weight (WF), weight gain (WG). The best growth performance was observed in fish 14% dietary lipid. Protein efficiency ratio (PER) and glucose of fish was not affected by interaction dietary protein and dietary lipid level. Triglyceride and cholesterol levels were significantly affected by protein and lipid and the interaction, High levels of triglycerides in fish fed with 30% protein and 10% and 12% lipid and the maximum amount of cholesterol in fish fed with 40% protein and 12% and 14% lipid were observed. The results of this study show a lower protein content (30%) and older (40%) than Kutum need can be a negative influence on the growth and physiological parameters. Increasing dietary lipid to 14% of Kutum juveniles were no negative effects on growth parameters. In order to improve the performance of Kutum in this weight range fed diets with 35% protein and 14% lipid is suggested.

In order to improving suitable growing condition for cultured species، knowledge of energy yielding nutrients، especially information about carbohydrate requirement are of oriental river prawn is essential، since there is not exclusive studies about carbohydrate requirement about this species، hence an nutritional experiments was conduct in order to find out the optimum level of carbohydrate. Five treatments with levels 15، 20، 25، 30 and 35 percent with iso- nitrogenous (35%) and iso- lipidic (8. 5%) we formulate each treatment was conducted in triplicate. Two hundred and twenty five juvenile prawn (of size 1. 5±0. 33g) were randomly distributed between 15 aquarium tank of 200 L capacity. The juvenile prawns which fed diet containing 30% carbohydrate were shown optimum condition in growth indices and was significant with other treatments (P < 0. 05>. growth Treatments 2 and 3 (20 and 25%) had not showed any significant differences in growth indices (P≥0. 05). The comparison of mean body chemical comparison of juvenile prawns carcass in tested diets in treatments 1، 2 & 3 had not showed significant differences in the amount of crude protein، lipid، total ash، and moisture (P>0. 05): however، treatments 1 & 5 had higher amount of moisture، with lower amount of protein and lipid value in their body composition (P<0. 05).

Effects of Shaheed Rajaee Dam constructed on the Tajan River on diversity and separation of Spirlin (Alburnides sp.) population was studied. Total of 100 fish samples including 50 samples from either side (below and above) of the dam were caught by electrofishing gear and examined. 53 morphometric were measured with the aid of digital clipper and 16 meristic (numerical) characteristics were also determined in all samples. Principal component (PCA) and detrended fluctuation (DFA) and cluster analyses were used to examine morphometric and meristic (numerical) data following standardization (to avoid error related to alometric growth). The one- way ANOVA analysis for standardized morphometric traits revealed statistically significant differences in 27 parameters within specimens which show relatively high phenotype diversity in A. bipunctatus from two studies areas. The DFA analysis showed that fish population in two areas below and above the dam are separated by 93% in morphometric and 78% in meristic characteristics. The PCA analysis also showed that 8 morphometric and 6 meristic characteristic separate fish populations of the river below and above the dam. The dendrogram constructed by UPGMA based on Euclidean distance coefficient also separated the fish population upstream and downstream of the dam.

This study was designed to evaluate population dynamic in the Tajan river and investigation Shahid-Rajaei dam effects upon Alburnoides sp.. A total 621 fish specimens during 9 month were collected by electeroshocker set that 508 individual related to up stream station and 113 individual related to down stream station. The mean fork length and weight of this species in upstream was 49.11± 11.95 cm, 3.29 ±2.69 gr and in downstream was 52.94± 11.93 cm, 3. 92 ±2.96 gr, respectively. Univariate analysis (One-Way ANOVA) of length between two stations showed significant differences while weight and condition factor only in male showed significant differences (P≤0.05). The age of the female samples in upstream of the dam in 4 class ranged from 0+ to 3+ years while other (male in upstream, male and female in downstream) samples in 3 class ranged from 0+ to 2+ years. Sex rate of male: female in up- and down stream were 57.9:42.1 and 61.3:38.7, respectively that shown female were dominant in two stations. We observed an isometric growth pattern in males caught in downstream and positive allometric in the rest of fishes andValue of condition factor in both stations were above of 2.

The effect of Pediococcus pentosaceus as a probiotic on the growth and immunological factors of the Siberian sturgeon, Acipencer baerii, was assessed. For this purpose, 180 fish specimens of 143.3±0.01 g body weight were randomly distributed in 12 fiberglass tanks (15 fish per tank) and fed with four diets containing 107, 108 and 109 bacterial cells per gram, respectively. The forth diet (control) contained no probiotics. Results showed significant improvement in FCR, PER, SGR and BWI in groups fed on diets containing probiotics compared to the control group. However, CF decreased in the probiotic- treated groups, though not significant. The result of immunological factors indicated increased lyzozyme activity, ACH50 and total igM. The best result from the assessment of immune factors was observed in the fish fed with 109 bacteial cell per gram of diet.

The aim of this study was to evaluate the spirulina powder in the diet of the southern Caspian kutum and compare with basal diet (lack of spirulina). The experiment was conducted for 90 days under controlled conditions. In this experiment، five commercial diets containing 0 (basal diet)، 0. 5%، 1%،2% and 5% spirulina powder was considered and fed to Triplicate groups of fish. Four-hundred and fifty fish with initial average weight of 0. 475±0. 081g were randomly distributed between 15 glass aquarium tank of 50 liters capacity which was filled with 40 liters of fresh filtered ground water. The fishes were fed at satiation at three times (8، 12 and 18). With increasing spirulina powder to 5% level all growth factors and fed efficiency were not improvement and was significant with other treatments (P<0. 05). The use of spirulina powder has position effect in the survival rate of kutum fry up to 2% level after that (5%) has been decreased. The chemical body composition has no differences in treatments (P>0. 05). In general، considering the growth factors، feed efficiency، the treatment 1 (commercial basal diet) is more reliable in the diet of southern Caspian kutum.