محمدرضا ایزدپناه

Breast cancer is one of the most important causes of cancer deaths especially in developing countries. However, some synthetic compounds such as thiosemicarbazone and its metal derivatives can play a very significant role in reducing the death rate from cancer. The aim of this study was to investigate alterations in the expression of BRCA1, p53 and Bcl-2 genes in breast cancer cell line.

In this study, the effect of pyrazole derivative of thiosemicarbazone (T) as well as T-bound iron oxide magnetite nanoparticles (T/ Fe3o4 np) were investigated on expression changes of Bcl-2, BRCA1 and p53 in MCF-7 cells. For this purpose, the toxicity of the compounds was first measured using the MTT reaction for 24 hr. Then, the mRNA expression levels were evaluated using real-time PCR reaction.

The results showed a high toxicity for the nano-pyrazole derivative of thiosemicarbazone magnetite so that; the fatality rate for treated thiosemicarbazone magnetite derivative cells was much higher than the compound without any nanoparticle. Also, real-time polymerase chain reaction analysis of gene expression indicated a high effect of the derivative on increasing the expression levels of BRCA1 and p53 genes and also decreasing the Bcl-2 gene expression of Bcl-2.

The use of anticancer compounds like thiosemicarbazone which bound to iron oxide nanoparticles would increase the toxicity of the anticancer compounds along with enhancment of delivery of them with high solubility to target tissue.

Several studies have compared surgical and conservative methods for the treatment of fingertip injuries. However, there are few studies on the effect of phenytoin dressing and its efficiency in treating the fingertips compared to other common treatments. The aim of this study was to compare phenytoin dressing, surgical treatment with VY flap technique and conservative treatment with Vaseline in patients afflicted with fingertip injuries. This prospective cohort research was conducted on all patients referred to the Emergency Department of Gorgan Hospital during 2016-17 who suffered from fingertip injuries. The eligible patients were selected for further intervention and follow up and were divided into three groups including phenytoin dressing, surgical treatment with VY flap technique and Vaseline conservative treatment. The collected Data was analyzed using Chi-squared test and independent sample t-test and the significance level was considered below 0.05. After 12 months, subjective, objective, and cosmetic complaints were evaluated based on the Allen Impairment Scale. According to the statistical evaluations, no significant difference was found between the three groups in subjective, objective, and cosmetic complaints after one year (P<0.05). The results of this study for the first time showed that phenytoin dressing has a similar effect to Vaseline dressing for the treatment of fingertip injuries and is not superior to it. Furthermore, the findings of this study, in agreement with previous studies, showed that clinical results are similar to conservative and surgical treatments and are not superior to each other.

Staphylococcus aureus is an important opportunistic pathogen causing a wide range of infections in human. Most clinical isolates of S.aureus are resistant to a number of antibiotics. For appropriate antimicrobial therapy, this study was conducted to determine antibacterial drug resistance patterns of S.aureus isolates obtained from different clinical samples in Rasht. the clinical isolates of S.aureus were collected from different clinical laboratories in Rasht. Thirty coagulase positive S.aureus strains were identified using biochemical tests and amplification of 23SrRNA and coa genes by polymerase chain reaction. Finally, the resistance pattern of the isolates to 16 selected antimicrobial agents was evaluated by disk diffusion method. the S.aureus isolates (75%) were resistant to methicillin and all of them were multidrug resistance. The isolates were high resistance to ampicillin (73%), amoxicillin (60%), cloxacillin (53%) and low resistance to vancomycin (7%) and gentamicin (10%). given the high prevalence of methicillin resistant, multi drug resistant and presence of vancomycin resistant S.aureus isolates in Rasht, continuously monitoring of drug resistance pattern of S.aureus isolates is recommended for having appropriate therapeutic regime.

Mechanical alloying technique is used for production of nanostructured soft magnetic alloys. In this work the back propagation (BP) artificial neural adopted to model the effect of various mechanical alloying parameters i.e. milling time and chemical composition, on the properties of Fe-Ni powders. Lattice parameter, grain size, lattice strain, coersivity and saturation intrinsic flux density are considered as the output of five BP neural networks. The results obtained show the efficiency of designed networks for the prediction of the properties of Fe-Ni powders.