دکتر علی گلچین

Scientific papers are valuable sources of information for scientific study. Apart from the main text, their content holds a notable amount of information. Recently, COVID-19 and related papers have attracted much attention between academic researchers, non-academic people, and diverse social media. These papers can reveal different aspects of the COVID-19 pandemic.  Publication criteria are changing rapidly for science-related coronavirus pandemic, as scientists worldwide conduct research at breakneck speeds to tackle this pandemic(1). During the last pandemic, we dived into the scientific literature to reflect on the COVID-19 pandemic. Researchers have created so much about COVID-19 related information that has been necessary for public health responses and the speedy development of therapeutic protocols and effective vaccines(2). To date, different therapeutic theories have been presented for treating and preventing COVID-19(3–6). However, the COVID-19 pandemic has severely discouraged people's lifestyle and economic situation. Therefore, many people, including lawmakers and directors from different agencies, have turned to the scientific society for answers regarding actions that need to be taken to control and treat the COVID-19(2). Nevertheless, many scientific studies published concerning the COVID-19; even those reported by prestigious journals fall short on experimental evidence to support their conclusions. Tremendous pressure existed to immediately know more about COVID-19 and how to stop this pandemic. Hence, the aforementioned situation created a unique atmosphere that led editors and reviewers to accept manuscripts that would have never been considered for publication under different conditions. During this pandemic, the piece of unreliable information was reported in journal papers.  Some of these journals are prestigious with considerable scientific impact factors. However, publishing this erroneous information was a negative point for these journals and their authors. Still, this erroneous information compared to shared inaccurate information in different electronic media, primarily virtual media, was debatable and valuable. In virtual media, many fortune-tellers were reputed due to sharing different predictions of the future of this pandemic. Many virtual channels shared their false profitable information.   Understanding developing areas of a multidisciplinary research field is critical for managing the COVID-19 pandemic. After the emerging COVID-19 pandemic, many multidisciplinary researchers focused on COVID-19 treatment and its impositions. Different medicine and biotechnology branches are mobilized to provide the best therapeutic approach for treating COVID-19 patients. In addition to traditional protocols, modern emerging fields such as cell-based therapy and personalized medicine also entered into this area(7,8). Hence, many studies began, and then many papers published in this regard. However, it is often difficult to understand their validity and efficacy with the vast amount of available online papers. This letter presents a brief analysis to interpret and visualize published papers related to COVID-19 during the last two years. Based on released information of the national center for biotechnology information (NCBI), 139114 journal manuscripts in Pub Med and 157482 journal manuscripts in PMC have been indexed (Figure-1)(9). Currently, the total number of published paper related to COVID-19 are increasing. However, publishing academic papers is one of the critical roles of academic societies that can present many advances in improving public health knowledge.

Bone allografts have become an accepted technology to replace bone loss due to common diseases, such as tumor resection, trauma, and failed total joint arthroplasty. This study briefly reviews a branch of regenerative medicine related to bone allografts and its related aspects. As new allografts stimulate an immune rejection response, bone grafts are usually prepared by freezing or freeze-drying to decrease the immune system responses. The notable biological events of associations, such as hemorrhage and inflammation, osteogenesis, osteoinduction, osteoconduction, and impressive remodeling, result in a load-bearing structure. Generally, cancellous allografts are effectively incorporated, while cortical grafts remain an admixture of viable regenerated bone and old necrotic bone for a prolonged duration. Massive bone allografts used in reconstructing tumor resection have a long-term successful outcome in about 80 percent of the procedures. Similar success has been reported for its use in total joint revision surgery and other clinical applications. The significant complications reported for grafting procedures are infection, bone graft fracture, nonunion at the graft-host juncture, and rare massive allograft resorption. Although bone allografting is a successful therapeutic approach, emerging technologies will introduce more efficient bones by mobilizing the adjunctive growth factors, cell and gene-based therapies, and tissue engineering techniques. The primary and applicable knowledge of bone allografts is essential; however, continual investigations are necessary.

Generally, cell interactions with the surface of biomaterials are mediated by chemical groups and biological intermediates. Cell behavior includes adhesion, proliferation, migration, differentiation, and function can be under the impression of cell-surface interaction. Therefore, Knowledge of cell-surface interaction is useful in the engineering and production of ideal cell-tissue based products. According to this important, this study was designed to review the interaction between cells and the surface of polymeric structures in cell culture conditions. This study gave an overview of the effects of key parameters on cell attachment, proliferation, migration, differentiation, and cell fate in culture. The cell-material surface interactions focus on the communication between surface properties of the substrate, protein/water adsorption, cell adhesion, and its function. Surface modification as compared to non-modified surface improves the intrinsic properties of a surface which yields a convenience of the biocompatible construct. However, the quality of notable parameters between cell and surface express the main role in tissue engineering, and subsequent can be led to high- quality cell-tissue based products.

Accumulating evidence indicates that toll-like receptor 4 (TLR4) plays a critical role in promoting adaptive immune responses and are definitively involved in the expansion and maintenance of the neuropathic pain. However, the application of docking in virtual-screening in silico methods to drug discovery has some challenge but it allows us to make the directed and meaningful design of drugs for a target protein, which can be shortening and low costing the evolution and discovery of very promising lead new drugs. Nevertheless, in parallel with virtual screening methods, attendant developments in cell culture and in vivo studies must be achieved. In the present paper, we aimed to discover new drugs that have the ability to bind and inhibit TLR4 functions. So, after using the Pathway studio to investigate the biological pathways and protein interaction maps between TLR4 and neuropathy, we reported the application of the affinity-based approach of different pharmaceuticals; these agents contained all of the approved drugs; which could bind to Toll-like receptor 4 in blind high-throughput in silico screening. Our results demonstrated that among the primary list of 1945 retrieved compounds, 39 approved compounds could be the right candidate to perform a biological test in different in vivo and in vitro conditions and as a lead for further neurophysiological and neuropathological studies and treatment of neuropathic pain.

Ammonium is produced in culture medium due to amino acids degradation and has adverse effect on in vitro culture of embryo. In the current study, the purpose was to evaluate the effects of ammuniom chloride (AC) on in vitro oocyte maturation rate and early embryo development in the sheep and its effect on the expression of Bcl-2.
In vitro maturation (IVM) was performed in the presence of various concentrations (0, 29, 88,132,176 μM/ml) of ammonium chloride (NH4CL) (AC). Meiotic maturation, embryonic development and expression of Bcl2 gene in Blastocyst cells were determined. The data were analyzed by one-way ANOVA and Tukey post HOC test, and values with p The highest concentration (176 µM) of AC significantly decreased the rate of fully expanded cumulus cells 24 hr after IVM compared with the control group (p Our results suggested that significant increase in IVM rate could be obtained with supplementation maturation medium with AC in a dose dependent manner. Increased AC concentration led to lower blastocyst rate under normal condition. However, regulation of pro–apoptotic (Bcl-2) gene did not change with different concentrations of AC supplementing.