jinous asgarpanah

The study aimed to explore the impact of methanolic extract from the medicinal mushroom Ganoderma lucidum (GLE) in various concentrations (0, 0.5, 1, 1.5, and 2%) on the rheological and microbial characteristics of UF-white cheese during a 90-day ripening period at a controlled temperature of 5±0.5°C. The study's findings indicated that the optimal combination of storage modulus, loss modulus, and loss tangent was observed in the group integrated with 0.5% GLE, regarding microbial analysis. However, the population of lactic acid (LAB) and mesophilic bacteria exhibited an incremental trend parallel to increasing GLE concentrations by up to 2%. Nevertheless, a reduction was observed during the ripening phase. Additionally, the growth of fungi was effectively inhibited in the presence of GLE, with the sample containing 2% GLE displaying complete fungal suppression compared to the control (6.32±0.12 CFU/g) over 90 days. Remarkably, no coliform bacteria were detected in any treatments throughout the ripening process.

Teucrium orientale is commonly used as an analgesic, antibacterial, antifungal, and antioxidant, protecting the liver and digestive system, and treating type 2 diabetes. Evaluation of anti-nociceptive and anti-inflammatory properties of ethanolic plant extract using animal models. The ethanolic extract was prepared using the maceration method from ground-dried aerial parts of the plant. Analgesic effects were determined by acetic acid-induced writhing and hot plate test on mice. The anti-inflammatory effect was evaluated using Carageenan and Cotton pellet tests on rats. In the writhing test, TOE (100, 200 and 400 mg/kg) caused a significant reduction in abdominal contractions. Inhibition percentages of abdominal contractions in test groups (100, 200 and 400 mg/kg), morphine and mefenamic acid were 87.40%, 91.80%, 98.29%, 94.40% and 96.89%, respectively, which indicate the visceral analgesic effects.In hot plate tests, the pain threshold significantly increased in test groups. Extract in the doses of 100mg/kg (P< 0.001), 200mg/kg (P< 0.0001) and 400mg/kg (P< 0.001) significantly reduced the paw edema in the carrageenan test at the second hour. In the cotton pellet test, the prescribed doses of the plant (100-200 mg/kg; P<0.0001) significantly reduced the formation of granuloma tissue and reduced the rate of edema. The percentage of inhibition of granuloma tissue by indomethacin and extract at doses of 100 and 200 mg/kg were 39.19%, 66.75% and 75.49%, respectively, and the percentage of inhibition of exudates were 26.5%, 55.57% and 67.24%, respectively. These results clearly showed the anti-nociceptive and anti-inflammatory effect of Teucrium orientale extract in animal models.

In the current study, the effect of different silicon dioxide nanoparticles (SiO2 NPs) concentrations was investigated on biomass yield, chlorophyll (Chl a) pigment, antioxidant enzymes, and defence metabolites of Spirulina platensis alga. The alga sample was cultured in the Zarrouk medium supplemented with various SiO2 NPS concentrations (0, 50, 100, and 150 mg L-1) for two weeks. Results showed that SiO2 NPs at 100 mg L-1 increased significantly dry weight, specific growth rate, and Chl pigment, possibly due to the induction of protein content and antioxidant enzyme activities of catalase and peroxidase. Secondary metabolites such as phycobiliprotein, phycocyanin, allophycocyanin, phycoerythrin, carotenoids, and extracellular polymeric substances increased upon all concentrations of SiO2 NP, although their contents were more increased under 100 mg L-1 treatment. Treatment of SiO2 NP at 150 mg L-1 induced toxicity in the algal growth along with the accumulation of H2O2, inhibition of antioxidant enzyme activities, and decline in the content of secondary metabolites. The findings suggest that 100 mg L-1 SiO2NPs is an optimum concentration for sustainable production of S. platensis, and may act as an elicitor to stimulate antioxidant metabolites for suppressing oxidative injuries.

 Nannorrhops ritchieana(N.ritchieana) is the sole species in the genus Nannorrhops in the palm family of Arecaceae. Regarding the use of N. ritchieana inflorescence (NER) in the folklore medicine of the south of Iran for relieving inflammation and pain in inflammation-based disorders, the current study assessed the anti-inflammatory features of N. Ritchieana inflorescence extract (NRE) in animal models.

 The carrageenan-induced paw edema method was used to evaluate theacute anti-inflammatory effects of the NRE extract (50,100, and 200mg/kg, IP). The standardand control groups received mefenamic acid and normal saline. Then, the rat’s paw tissues were removed and collected for studying tumor necrosis factor (TNF-α) level. In addition, the cotton pellet-induced granuloma method was utilized to assess the chronic anti-inflammatory effects of the extract (100 and 200 mg/kg IP). Standard and control groups received indomethacin and saline. On the last day of the cotton pellet test, the rat’s serum was collected for interleukin-1 beta (IL-1β) determination.

 In the carrageenan test, the extract significantly reduced the paw edema compared to the control (p<0.05). The extract’s anti-inflammatory activity (100 and 200 mg/kg) was the same as the mefenamic acid group. TNF-α levels demonstrated significant anti-inflammatory effects just at the dose of 200 mg/kg. In granuloma induced by cotton pellets, NRE was also effective based on the granuloma formation and the transudate amount. The anti-inflammatory activity of the inflorescence extract(100 and 200 mg/kg) was found to be the same as the indomethacin group, whereas IL-1β did not represent significant changes.

 According to the results, NRE had a considerable anti-inflammatory effect.

Pycnocycla bashgardiana is used in Iranian traditional medicine for the relief and treatment of pain and inflammatory disorder. This study investigates the anti-inflammatory and anti-nociceptive activities of P.bashgardiana essential oil (PBEO).The anti-inflammatory effect of PBEO (50, 100, and 200 mg/kg, i.p.) were determined using carrageenan test in rat. The analgesic activity of PBEO (20, 50, 100, and 200 mg/kg, i.p.) was studied by formalin test in mice. PBEO (50, 100, and 200 mg/kg) significantly (p<0.05) inhibited the carrageenan-induced paw edema which was observed at the 3rd hour of the experiment by 43, 64 and 58%, respectively. PBEO showed significant anti-nociceptive effects in the first phase (200 mg/kg, 40% pain inhibition) and the second phase (100 mg/kg, 37% pain inhibition) of formalin test. According to the GC-Mass spectrometry findings, myristicin (21.1%), cis-isomyristicin (17.2%), E-β-ocimene (11.1%), and Z-β-ocimene (6.2%) were characterized as the main components. The results suggest that PBEO could be a potential candidate as an anti-inflammatory and anti-nociceptive agent in the management of inflammation-based disorders. These effects might be partially due to possible inhibition of or interference with the production of some inflammatory mediators.

Pycnocycla bashagardiana essential oil (EO) is extracted from fruits via hydrodistillation and analyzed through gas chromatography-mass spectrometry.

This study aimed to evaluate molecular docking and cytotoxicity by P. bashagardiana EO in colon cancer cell lines.

The viability rates of P. bashagardiana fruit essential oil (PBFEO)-treated normal L929 and HT29 cancer cells were assessed at 0 - 500 g/mL in 24 and 48 h by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The cell apoptosis percentage was investigated via flow cytometry and gene expression of BAX and BCL2 using the real-time polymerase chain reaction. In addition, the efficient myristicin binding was examined with the BCL2 protein through molecular docking analysis.

The half maximal inhibitory concentrations of 431.8 and 450 g/mL in L929 cells, as well as 278.5 and 144.6 g/mL in HT29 cells, were recorded at 24 and 48 h, respectively. The cell apoptosis percentage was examined by annexin-V-FITC. The results indicated a significant increase in the apoptosis percentage. A significant increase was also observed in HT29 cancer cells compared to normal L929 cells regarding the expression ratio of BAX/BCL2 genes. Myristicin’s binding affinity to BCL2 was relatively weak compared to the control.

Based on the results, myristicin can be suggested as a complementary treatment for colon cancer through more effective treatment and reducing the side effects of normal cells.

The current study aimed to optimize the extraction conditions from Ganoderma (G) Lucidum mushroom by ultrasound in order to increase the extracted phenolics and antioxidant compounds. The impact of solvent type (water, methanol, and 50:50% combined solution of both), time (5, 10, 15 min), and ultrasound intensity (100, 200, and 300 W) on the extraction yield of phenolics and antioxidant compounds from G. lucidum mushroom were investigated. The Response Surface Method (RSM) was used to optimize the extraction conditions. In the single optimization condition, the maximum total phenolics (36.6989 mg/g) extraction yield from G. lucidum was achieved in 15 min extraction time, 300 W ultrasound power, and the use of methanol solvent. The lowest IC 50 (0.8983 mg/mL) was observed in the extraction time of 10 min, the ultrasound power of 300 W, and the use of methanol solvent. Multiple optimizations of extraction conditions from G. Lucidum to achieve the highest total phenol (36.6989 mg/g) and the lowest IC 50 (0.9413 mg/mL) were predicted in 300 w ultrasound power, 15 min, and the use of methanol solvent. No significant difference was observed between the predicted and experimental results.

Medicinal plants are rich in secondary metabolites and contain the active ingredients of many drugs(Davari et al., 2018). Over the years, the secondary metabolites in plants have been used to treat most diseases due to their ease of access and use and fewer side effects compared to chemical products(fazelinasb et al., 2017). Fungi are one of the types of medicinal plants that have attracted the interest of researchers in recent years. Ganoderma Lucidum is an annual fungus of the genus Basidiomycetes belonging to the class Agaricomycete, order Ployporaceae and family Ganodermataceae(chen et al., 2017). This effective medicinal fungus has been khown in East Asian countries for about 2000 years, such as Reishi or Mannetake in Japan(ten thousand years old fungus) and Ling Chih, Ling Chu and Ling Zhi(immortal fungus) in China and Korea is famous(Robertos 2004). Medicinal properties of Ganoderma Lucidum are due to the presence of biologically active compounds such as polysaccharides, sterols, amino acids, triterpenoids, alkaloids, proteins and peptides(Peterson 2006). As triterpenes and polysaccharids are the most important chemical compounds in Ganoderma that most scientific research has been done on them(stemets 2000).One of the most important polysaccharides in Ganoderma lucidum is Glucan, which strengthens the immune system(wang et al., 2002). The polysaccharides in Ganoderma lucidum also have antioxidant, antibacterial, antiviral, and radiation protection properties, and the protein in it has anti-tumor and anti-diabetic activity(Gao et al., 2004). Ganoderma lucidum contains a variety of compounds, including ketones, esters, lactones, alcohols, ethers, and hydroxybenzene (Taskin et al., 2013).The polyphenolic portion of Ganoderma lucidum consists mainly of flavonoids such as Quercetin, Routine, Myersitin, Morin, Hesperetin, and Naringenin (Saltarelli et al., 2015 , Veljovic et al., 2017).Different methods are used to extraction plant extracts such as ultrasound, microwave, supercritical fluid and maceration. Each of the obove methods has a differents ability in extracting plant compounds, depending on the sample, type of solvent used and extraction conditios.In the maceration method, the mass of the desired compound is transferred from the sample to the solvent used while the solution is in contact with the sample. Extraction also depends on the particle size and properties of the plant tissue. Extraction efficiency in maceration method is generally higer than new extraction methods and the probability of heat damage in this method is lower(wang et al 2002).

Ganoderma Lucidum was obtained from the Department of Medicinal Plants of the University of Tehran. The materials used for the tests, including ethanol and methanol solvents, were purchased from Pars Shimi Company, Iran. Folin ciocalteu reagent was purchased from Merk Company, Germany. Galic Acid, DPPH reagent, and sodium carbonate were purchased from Sigma Company, USA.Ganoderma lucidum was extracted by using the Shon method with modifications.The total amount of phenolic compounds in the Ganoderma lucidum extraction was measured by the Folin-Ciocalteu method. In this method, Folin reagent is reduced in the presence of phenolic compounds in alkaline solution and blue color is produced in the solution. Color intensity can be determined at a wavelength of 765 nm by the optical spectrometer.Evaluation of free radical inhibition activity with Diphenyl Picrylhydrazyl was measured. The IC50 factor, which represents the amount of required sample to inhibit 50 percent of free radicals, was used for better assessment of antiradical activity. The lower the IC50, the higher would be antioxidant potential (low concentrations of the sample can prevent large amounts of free radicals [ Khalili and Ebrahimzadeh 2015]. Minitab software version 16 was used to design the treatments from the response level method, the Box- Behnken model, and to assess the optimal conditions for extraction from Ganoderma Lucidum by the statistical method of the response level.

different extraction conditions (tempreature, time, and type of solvent)had significant effects on the total amount of phenolic compounds and IC 50 of Ganoderma lucidum extract, so that the total amount of phenolic compounds of produced extract from Ganoderma lucidum ranged from 7.244 to 21.089 mg100g-1, and IC 50 ranged from 2.409 to 8.369 percent. The results show that increasing temperature (20 to 40 C°), extraction time (12 to 48 h), and application of methanol solvent, compared to the use of water solvent and a mixture of 50/50 water and methanol, significantly (P ≤ 0.05) increases the total extraction of phenolic compounds and decreases the amount of IC 50 from Ganoderma Lucidum.The linear effects of all three variables of temperature, time and type of solvent, and square effect of solvent type (C2) on the total extraction of phenolic compounds and IC 50, were significant (P≤ 0.05). Meanwhile, the square effects of temperature, extraction time, interactional effects of temperature, extraction time, solvent type on the total extraction of phenolic compounds, and IC 50 from Ganoderma lucidum were not significant (P <0.05).According to Single optimization the maximum amount of phenolic composition of the produced extract from 20.9944 mg100g-1 Ganoderma lucidum with 99.32 percent desirability was achieved in the following condition: 48 h of extraction time, 40 C° of temperature, and the use of methanol solvents and the maximum antioxidant composition of the produced extract from Ganoderma lucidum which can inhibit free radicals was predicted to be 2.1128 percent with 100 percent utility belonging to 48 h extraction time, 40 C° temperature, and use of methanol solvent. According to Multiple optimizations total phenol optimum and IC 50 of the produced extract from Ganoderma lucidum. It was predicted that the optimal conditions for the produced extract from Ganoderma lucidum by macration method to achieve the maximum total phenolic composition and the minimum amount of IC 50 multiple with 99.662 percent of desirability on 48 h of extraction time, 40 C° of temperature and the using of methanol solvent that total phenol content and the IC50 were 20.9944 mg100g-1 and 2.1128 percent, respectively.

The results of this study proved that by optimizing the extraction conditions of Ganoderma Lucidum, a higer amount of its effective compounds can be achieved. Also, the response surface method can be an effective way to optimize the extraction conditions of effective compounds from Ganoderma Lucidum.

The aim of this study was to evaluate the anti-inflammatory effect of B. persicum essential oil on colonic inflammation and the role of suppression of NF-κB pathway in rat colitis induced by acetic acid solution. Induction of acute colitis was done by intra-luminal instillation of 2 ml of acetic acid (4%) diluted in normal saline. Two hours after colitis induction, 0.2% tween 80 in normal saline, prednisolone (4 mg/kg) or B. persicum essential oil (100, 200, and 400 mg/kg) were administered to the rats orally and continued for 5 consecutive days. The severity of macroscopic and microscopic damages was assessed. Myeloperoxidase and TNF-α activity was evaluated by biochemical analysis and ELISA respectively and protein expression of p-NF-κB was assessed by immunohistochemistry (IHC). Prednisolone and B. persicum essential oil (100, 200, and 400 mg/kg) decreased macroscopic and microscopic injuries compared to the acetic acid group. On the other hand, prednisolone and B. persicum essential oil (200 and 400 mg/kg) decreased the activity of MPO and TNF-α in the colon tissue of rats compared with the acetic acid group. Furthermore, they suppressed the expression of p-NF-κB protein induced by acetic acid administration. It is suggested that the anti-inflammatory effect of B. persicum essential oil on acetic acid-induced colitis in rats may be due to the suppression of NF-κB pathway.

The species of Cymbopogon are generally used for different pharmacological effects. No histopathological study has been conducted on the plant’s toxicity so far. Thus, the acute and repeated toxicity of Cymbopogon essential oil were investigated.

The essential oil from aerial parts of Cymbopogon schoenanthus was administered in mice by gavage in both acute and repeated models. The animals were then divided into control and test groups. In the acute toxicity, 2000 mg/kg C. schoenanthus essential oil was administered in mice. Death rate, toxic symptoms, body weight, and abnormal behaviors were also observed for 14 days. In the repeated toxicity, C. schoenanthus essential oil (10, 100, and 200 mg/kg) was daily administered for a 4-week period. On the 28thday, all animals were sacrificed and their blood and tissue samples were prepared. Moreover, clinical, biochemical, and histopathological changes were compared to the control group.

No mortality was noticed in the acute test; therefore, the oral LD50 value was determined to be greater than 2000 mg/kg in the female mice. In the repeated test, the animals were given C. schoenanthus essential oil, which consequently showed no mortality and toxic symptoms. The repeated administration of C. schoenanthus essential oil had a variation on glucose, urea, Na+, and K+ levels. Moreover, the terminal necropsies revealed low toxic effects on the liver.

The results indicate that the oral acute toxicity of C. schoenanthus essential oil in mice was of a low order with LD50 being more than 2000 mg/kg. Additionally, slight tissue damage to liver was observed when it was administered sub-chronically at the dose of 200 mg/kg.

 Regarding the high prevalence of cancer in Iran and the cytotoxic properties of T. polium, the current study aimed to investigate the cytotoxic and anti-mutagenicity effect of T. polium essential oil (TpEO) on human colon adenocarcinoma cell line (HT29).

 HT29 cells were cultured in L-glutamine, RPMI Sigma (1640), with 10% of FBS (fetal bovine serum). Then, the cultures were incubated with 5% CO2 at 37°C, and MTT assay was used to recognize cell proliferation under the inhibitory influence of TpEO. The cell cycle progression was monitored by Sub-G1 apoptosis assay using flow cytometry. Eventually, the anti-mutagenicity property was evaluated using the Ames test employing TA100 and exposure to sodium azide as the carcinogenic substance.

 The cytotoxic effect of TpEO on HT29 cells was 66.867 ± 1.37 µg/mL. Cultured HT29 cells treated with TpEO exhibited morphological features of apoptosis. TpEO preventive effect was about 78.18%.

 This study showed that TpEO may be useful for treating colon cancer.

Vulvovaginal candidiasis, as a prevalent vaginitis, is developed by Candida albicans. Regarding to the high incidence of this fungal infection and the antifungal activities of Cuminum cyminum, the preparation of vaginal suppositories containing its essential oil, has been considered as the main goal of the present investigation.

The constituents of C. cyminum essential oil were identified by gas chromatography-mass spectrometry. The minimum inhibitory concentration (MIC) of the essential oil and the related formulation were determined by the cup plate method.

Vaginal suppositories containing 0.1 ml of C. cyminum essential oil per suppository base (2 gr) (Polyethylene glycol) were prepared and their appearance, mass uniformity, hardness, disintegration time, content and uniformity were evaluated. The stability of suppositories containing C. cyminum essential oil stored at 4, 25 and 40°C was investigated after 1, 2, and 6 months. It was found that storage at lower temperatures (4 and 25°C) did not cause any damages to the formulation.

Due to the high quality of the formulated suppositories, it would be suggested as a successful replacement in the treatment of vaginitis caused by Candida albicans, after approving its efficacy in future clinical studies.

In this study, the effects of different concentrations of silica nanoparticles (NPs) were studied on growth, membrane stability, and antioxidant properties of Matricaria chamomilla in vitro. The sterilized seeds were incubated in different concentrations of silica NPs (0, 2, 4, 6 g L-1) for one hour and then were cultured on Murashing and Skoog medium. Silica NPs application enhanced relative water content and fresh and dry weight of leaf and root. The highest growth was observed at 4 g L-1 silica NPs. Hydrogen peroxide and malondialdehyde significantly reduced at 4 g L-1 silica NPs. Total phenol and flavonoid contents increased by silica NPs treatment, and induction effect of silica NPs was more prominent at 6 g L-1 silica NPs. Low level of IC50 was detected at 6 g L-1 silica NPs. Overall, application of silica NPs at proper concentration can improve growth and induces the production of metabolites in M. chamomilla.

Salvia sharifii Rech. F. & Esfand (Lamiaceae) is an Iranian endemic plant that widely used as a medicinal plant. Due to the antinociceptive and anti-inflammatory effects of other Salvia species, the present study was conducted to investigate the antinociceptive effect of S. sharifii seed's oil (SSSO) in experimental animals. Seed's oil of S. sharifii was extracted by Soxhlet method. In order to assess the antinociceptive activity, the animals were classified to five groups: control (vehicle), S. sharifii seed's oil (20 and 100 mg/kg) and standard groups (morphine 5 mg/kg and mefenamic acid 30 mg/kg). The analgesic activities were studied by formalin test in mice. The nociceptive responses were divided to two phases; phase I (0-15minutes) and phase II (15-60 minutes). Comparisons between the groups were carried out using the analysis of variance (one way ANOVA) and post hoc Tukey's test. P<0.05 was considered as significant difference. S. sharifii seed's oil showed significant antinociceptive effects in first phase (dose 20 mg/kg) and second phase (dose 20 and 100 mg/kg) of formalin test compared to control group. These effects were comparable to morphine and mefenamic acid in chronic phase of formalin test. The results indicated that S. sharifii seed's oil induced a significant antinociceptive in mice that is likely related to active constituents of this seed's oil.

Pelargonium graveolens L'Hér. is an essential oil bearing plant used in traditional medicine of Iran for the treatment of inflammatory disease and pain, anxiety, sadness and also for the relief of gastrointestinal symptoms. Its pharmaceutical product is presently used in Iran for the treatment of depression disorders. Since colitis is a brain-gut interactive disorder, the authors were prompted to investigate the preventive effect of P. graveolens essential oil product (Deproherb®) to control the induced inflammatory bowel disease (IBD) in rats.

Experimentally ulcerative colitis was induced by acetic acid in animals pretreated with three different doses of Deproherb® (100, 200 and 400 mg/kg, p.o.) for five consecutive days. Anti-inflammatory effects of Deproherb® were compared with orally administrated sulfasalazine (4 mg/kg). Biochemical [Myeloperoxidase (MPO) activity] and macroscopic and microscopic examinations of the colon was performed. Deproherb® was also analyzed by GC and GC/MS in order to identify the potentially responsible compounds for observed property.

The obtained results indicated that the activity of MPO increased in acetic acid-treated groups, while it was recovered by pretreatment with Deproherb® (100-400 mg/kg) and sulfasalazine. All doses of Deproherb® and sulfasalazine-treated groups showed significant lower score values of macroscopic and microscopic characters when compared to the acetic acid-treated group. It was concluded that Deproherb® inhibited the acetic acid toxic reactions in the rat bowel.

The present study proved the anti-inflammatory potential of Deproherb® Oral Drop, in the experimentally induced colitis. Proper clinical investigations are suggested to confirm the observed activities in human.

Mentha mozaffarianii, an endemic species from the Labiatae family, is used in Iranian traditional medicine. This study evaluated the acute and repeated oral toxicity of the Mentha mozaffarianii essential oil (MMEO) in rats and mice. To assess the toxicity profile of the MMEO, we administered the essential oil to 48 rats and mice of both sexes by gavage in acute and repeated models. In acute toxicity, the animals were administered the MMEO (2000 mg/kg) and were monitored for 14 days. In the repeated toxicity, the MMEO was administered (100 mg/kg) daily for 4 weeks. On the 28th day, all the animals were scarified and blood and tissue samples were prepared. All the clinical, biochemical, and histopathological changes were assessed and compared with those in the controls. Statistical significance was determined by one- and two-way analyses of variance, followed by the Tukey test using GraphPad Prism 6. In the acute test, there was no mortality; therefore, the oral LD50 value determined in the mice and rats of both sexes was greater than 2000 mg/kg. In the repeated test, the animals received the MMEO and there was no mortality. In the biochemical analysis, there were significant increases in blood glucose, cholesterol, ALT, AST, ALP, and TSH in the female rats and also in BUN in the male rats. The histopathological studies revealed evidence of microscopic lesions in the liver, kidney, stomach, and small intestine tissues of the MMEO group. The results indicated that the acute toxicity of the MMEO in the mice and rats was of a low order and it revealed slight tissue damage to several organs when given subchronically at a dose of 100 mg/kg.

Pycnocycla bashagardiana is an endemic species found only in Iran. Due to the presence of myristicin as the major component of the fruit’s oil we were prompted to assess the antinociceptive and anti-inflammatory properties of P. bashagardiana fruit’s essential oil (PBFEO).
The analgesic activities of PBFEO (100, 200, and 400 mg/kg, IP) were studied by hot-plate and formalin tests in mice. Control and standard groups received vehicle and morphine (5 mg/kg, IP), respectively. The acute anti-inflammatory effect of PBFEO (200 and 400 mg/kg, IP) were assessed by carrageenan-induced paw edema method in 30 min, 1, 2, 3, and 4 hr after carrageenan injection and the chronic anti-inflammatory effect of PBFEO (50 and 100 mg/kg, IP) were assessed by the cotton pellet-induced granuloma method in rats.
In hot-plate and formalin tests, the studied doses of PBFEO were not effective. However, in carrageenan test, all studied doses ofPBFEO significantly reduced the paw edema in comparison to the control animals (P The results suggest that PBFEOpossesses biologically active constituents that have significant peripheral anti-inflammatory effects.

Increased microbial resistance to conventional medicines and their side effects have led to studying the effect of herbal extracts on microorganisms.
The current study aimed at evaluating the antimicrobial activity of total extract and fractions of chloroform, methanol, and aqueous of aerial parts of Salvia aegyptiaca.
The plants were collected from Bandar Abbas, Iran, in April 2015. The methanol extract of aerial parts, in addition to chloroform, methanol, and aqueous fractions, were prepared by the maceration method from S. aegyptiaca. The antimicrobial activity of fractions were determined by cup plate and further micro-dilution methods based on the Clinical and Laboratory Standards Institute (CLSI) 2013 against some Gram-positive and -negative bacteria as well as Candida albicans. Statistical analysis was conducted by t test.
Significant results were obtained regarding the tested microorganisms only by micro-dilution method.
There were no significant results in plant extract and its fractions against the tested microorganisms in the cup plate method, which may be due to the inability of agar diffusion. In contrast, by excluding the agar’s hindrance property, verified results of micro-dilution method were deleted.

Nepeta depauperata Benth is an endemic species and is extensively exploited as an anti-inflammatory agent in Iranian traditional medicine..
This study was designed to evaluate the antinociceptive activity of methanol extract of N. depauperata in male mice..
The anti-nociceptive activities of the extract were investigated by the formalin test and Hot plate test respectively. Comparisons between the groups were carried out using one-way analysis of variance (ANOVA), and post Hoc Tukey test..
N. depauperata extract showed anti-nociceptive effect. Doses of 50, 100 and 200 mg/kg reduced the paw flexing time in formalin test from the control (P This study revealed that the methanol extract of N.depauperata may minimize both the acute and chronic forms of nociception and may have potent role against inflammation..

Glaucium vitellinum is an endemic species and is extensively exploited as an anti-inflammatory agent in Iranian traditional medicine.
This study was designed to evaluate the antinociceptive activities of G. vitellinum methanol extract in male mice.
The formalin and hot-plate methods were used for pain evaluation in mice. Glaucium vitellinum extract (50, 100, 200 and 400 mg/kg body weight IP), saline and morphine (2 mg/kg, IP) were administered 15 minutes prior to the formalin test. The nociceptive responses were divided to two phases; phase І (0 - 15 minutes) and phase ІІ (15 - 60 minutes) were compared to the control and morphine. In the hot-plate test, G. vitellinum extract (80, 160, 200 and 250 mg/kg IP), saline and morphine (5 mg/kg, IP) were administered and, behavioral responses were immediately tested, 15, 30, 45 and 60 minutes after the injection. Comparisons between the groups were carried out using the analysis of variance (ANOVA), and post hoc Tukey’s test.
All doses of G. vitellinum extract induced anti-nociception activity during the first and second phases of the formalin test. The extract showed a significant (P This study revealed that G. vitellinum extract possessed a significant anti-nociceptive activity in formalin pain models and hot-plate test in mice and might have a potent role against pain.

Psoriasis is an autoimmune and recurrent chronic inflammatory skin disease. About 1-3% of the world wide populations are affected. The characteristic features are hyperprolifration of keratinocytes leading to redness, thickening and scaling of epidermis followed with itching and appearance of the lesions which in most cases bother the patients medically and psychologically. Psoriasis is symptomatically treated by the range of oral and topical medications, however, major side effects in some cases are associated with them. Based on several studies, Curcuma longa can inhibit several inflammatory enzymes mainly involved in the inflammatory process of Psoriasis. Therefore, we decided to target this well-known herbal agent with fantastic safety profile to be formulated as a novel topical microemulgel. The clinical and therapeutic benefit of this novel topical formulation was evaluated on 34 patients with mild to moderate plaque psoriasis in a randomized, prospective intra-individual, right–left comparative, placebo-controlled, double-blind clinical trial. The Dermatology Life Quality Index (DLQI) Questionnaire and Psoriasis area & severity index (PASI) score as well as photos before and after treatment was used to evaluate the outcome. The results show that the clinical and quality of life features in treated lesions in comparison with untreated lesions have improved (P<0.05). The reported side effects were also recorded and were trivial. Based on our findings, the proposed microemulgel may well be considered as an alternative in some patients and most likely as an add-on therapeutic option for many patients suffering with plaque psoriasis.

Belonging to Papaveraceae family, Glaucium vitellinum is one of the Persian endemic plants which has not been investigated biologically. The present paper focused on the assessment of the antibacterial and antifungal activities of the total methanol extract and alkaloid sub-fraction of the flowering aerial parts of G. vitellinum. The antibacterial and antifungal activities were investigated using cup plate method and disc diffusion assay, respectively. The MIC values of the active samples were determined using micro plate dilution method. The crude extract and alkaloid sub-fraction of G. vitellinum had significant inhibition activity on the growth of S. aureus and S. typhi. From antifungal assay, it is concluded that only the yeast C. albicans, showed a high sensitivity to the extract and especially to the related alkaloid sub-fraction. Regarding the results, G. vitellinum could be employed as a natural antibacterial and antifungal agent against S. aureus, S. typhi, and C. albicans, respectively. Moreover, based on the results of this study, further in vivo and ex vivo confirmatory tests for total methanol extract and alkaloid sub-fraction are recommended.

Belongs to Rosaecea family, Rosa foetida is one the Persian native plants which has not been investigated biologically. As it is traditionally used topically as poultice to treat infectious skin burns, the present paper focused on the assessment of the antibacterial activities of different extracts of R. foetida flowers against the main cause of skin burn wounds infections, Pseudomonas aeruginosa. The antibacterial activity and MIC value determination were investigated by cup plate method and micro plate dilution method respectively. All R. foetid extracts had inhibition activity on the growth of P. aeruginosa of which the aqueous and methanol extracts exhibited the strongest activities. Inhibition zone diameter and MIC values of the concentration of 125 mg/ml of both extracts were found to be somehow the same as those of the standard drug, Imipenem/Cilastatin (8/8 μg/ml). Results demonstrated that the plant is effective against the standard and pathogenic strains of P. aeruginosa and could be a potential source of effective natural antibacterial compounds to be applied in further phytochemical and invivo biological studies.

Nepeta depauperata belongs to the Lamiaceae family and is one the Persian endemic plants which has not been investigated biologically. In the present paper we had focused on the assessment of the antibacterial and antifungal activities of the total methanolic extract and different sub-fraction of the flowering aerial parts of it. The Antibacterial and antifungal activities were investigated by cup plate method and disc diffusion assay, respectively. The minimum inhibitory concentrations and minimum bactericidal concentrations of the active extract or subfraction were determined by micro plate dilution method. The crude extract and chloroform sub-fraction of N. depauperata had inhibition activity on the growth of Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa while no antibacterial activity observed against Staphylococcus epidermidis, Escherichia coli and Salmonella typhi. It was concluded from the antifungal assay that just the yeast C. albicans, showed a high sensitivity to all the extract and related sub-fractions. No activity was seen against Aspergillus niger, A. fumigatus, A. flavus and Fusarium oxysporum. These findings demonstrate that the N. depauperata is effective against S. aureus, B. subtilis and P. aeruginosa and could be a natural source of effective natural antifungal compounds against C. albicans.

Phlomis lanceolata is a medicinal plant that has long been used to treat various conditions such as diabetes, gastric ulcer, hemorrhoids, inflammation and wounds. As most of Phlomis species have shown cytotoxic activity against proliferation of different cell lines, a biological investigation of P. lanceolata was carried out in this study. The aim of this study was to find out the in vitro cytotoxic activity of total extract and different fractions of Phlomis lanceolata on four cell lines. Cytotoxic activity of the metanolic total extract and partition fractions of chloroform, ethyl acetate and petroleum ether of flowering aerial parts of Phlomis lanceolata on the HT29, Caco2, T47D and NIH3T3 cell lines is examined by MTT. Petroleum ether fraction showed high cytotoxic activity against proliferation of all four cell lines. Presence of heavy triterpens and lipophil compounds recognized by TLC test in Petroleum ether fraction is responsible for high cytotoxic activity. The results emphasize the importance of phytochemical studies which could lead to the discovery of new active compounds.