جستجوی مقالات مرتبط با کلیدواژه « رحم » در نشریات گروه « علوم پایه »

Inroduction &

The importance of histophysiological and histochemical study of the uterus, cervix and vagina in mammals is due to pathological examinations of these organs in infectious and metabolic diseases and infertility treatment. The aim of this study was to investigate the histophysiological and histochemical features of these organs in guinea pigs.

Five female guinea pigs with mean weight were obtained from laboratory animal breeding center and after sampling, Samples were stained by Hematoxin - Eosin, Periodic acid Schiff and alcian blue methods and then histological and histochemical properties of samples were samples were studied by light microscope.

The horn and the body of the uterus in general have three layers of endometrium , myometrium and primetrium. The endometrical epithelium in the uterine horn consists of simple cubic cells and it consists of simple cubic cells and simple columnar cells in the body of uterus. The endometrium has many coiled tubular glands. In the body and horn, the myometrium has 2 layers of longi tudinal and circular smooth muscle from outside to inside. The cervical mucosa has simple columnar cells. And has 3 types of folds: primary, Secondary and tertiary folds. Among the columnal cells, a large number of mucus secreting goblet cells are seen. The squomous vaginal epithelium is stratified stratum Corneum. no glands were observed in mucosal layer of the vagina.

The results show that the anatomical a tissue structure of Guinea pig uterus, cervix and vagina, despite minor differences, are very similar to other mammals.

The aim of this experimental study was to investigate the effective infertility factors of physoaline compounds on the adult reproductive system of Balb/C adult female.

The above experiment was performed by preparing the aqueous extract of the plant and selecting three doses of 7.5g/kg,9g/kg and 15g/kg;Simultaneously with the experimental groups,the distilled water sham group was injected and the control group was kept intact.Vaginal stromal smear cycles were prepared to examine different stages.One hour after the last injection,the mice were given blood samples and then killed by chloroform and their uterus and ovaries were prepared for histological studies.

Statistical studies have shown that injecting 7.5g/kg of extract reduces estrogen and restores 33% of fertility in mice. Also, at a dose of 9 g/kg, the mice were initially infertile, but one month after the last injection, 16.5% of the mice showed a return to fertility and a significant increase in progesterone.

According to the results, it is probably because physalines have a pseudo-steroidal structure. The receptor is occupied by physaline and can cause abnormalities in the growth and development of oocytes, uterine tissue cells and ovaries, and infertility.

antioxidants prevent the occurrence of unwanted factors in the female reproductive system by absorbing free radicals in damaged cells which may cause damage to the cell's enzymes and DNA changes and infertility.Propolis is a resinous substance collected by honeybees from some plant sources to disinfect the hive's environment.The propolis is rich in antioxidants. Given that the infertility rate due to the production of oxidative esters has increased dramatically in recent decades, and on the other hand, propolis acts as an antioxidant.

There are five mouse groups in this study:Control group (Without receiving alcoholic extract of propolis), Sham group (receiving 1 cc distilled water for 30 days), experimental groups 1, 2 and 3 (Under the influence of concentrations of 0.75, 0.57 and 0.18 mg / ml of alcoholic extract of propolis, 1 cc for 30 days). All mice were autopsied after gavage completion and their uterus and ovaries were isolated and for final examinations were prepared for microscopic study after taking the necessary steps.

Propolis being a strong antioxidant, increased the number of all follicles except the atritic follicles and hence uterine layers of the endometrium and primetriumwere increased and the myometrial layer decreased.

Gavage of propolis alcoholic significantly is effective on ovary follicls and uterus tissue Keywords: Alcoholic extract of propolis, Oogenesis, Uterus, Ovary

Different nutrients with effects on male reproductive organs have a significant effect on the prevention and treatment of infertility, one of these elements is selenium. The goal of this study was to evaluate selenium nanoparticles (NSAs) in oogenesis and uterine changes in adult female NMRI mice.In this study, 30 adult female NMRI mice were selected and randomly divided into five groups: controls, Sham and 3 experimental groups. Control group did not receive any material, the sham group received solvent and the experimental groups received nano selenium in 3 doses of 0.2 mg/kg, 0.3 mg/kg, 0.4 mg/kg for 30 days. The weight of the animals in the experimental groups at the high dose was significantly reduced. The weight and diameter of the ovary declined so that the results were not significant. The number of primordial follicles, primary and secondary follicles, and atretic follicle have decreased. The number of Graafian follicles decreased at a dose of 0.4 mg/kg of nano selenium and also corpus luteum in a dose of 0.3 mg/kg of nano selenium decreased. The diameter of the secondary follicle was increased, but not significant. The diameter of the Graafian follicle did not change. The number of blood vessels in the ovaries was increased, but the diameter of them did not change significantly. In experimental groups, the diameter of the uterine cavity was significantly reduced. The thickness of the perimetrium of uterine layer was reduced. The number of uterine glands endometrium decreased in the third experimental group, but their diameter did not significantly change in any of the groups. Nano-selenium caused an increase in the number of primordial, primary and secondary follicles due to its antioxidant effect, and the uterine layer of the endometrium increased and perimetrium and myometrium decreased.

Artemisia absinthium (known as wormwood) is used as a menstruation and abortive drug in the traditional medicine. Therefore, we have evaluated the effects of A. absinthium hydroalcoholic extract on the uterus, oviduct tissues and pituitary_gonadal axis hormones in adult female NMRI mice. In this experimental study, intraperitoneally injections were performed over 30 days on 50 female NMRI mice with 50, 100, and 150 mg/kg.bw doses of the extract of Artemisia stem and flower. Sham group received distilled water and control group was kept without any injection. After 30 days of injections, animals were dissected and blood samples were collected by heart aspiration. The levels of LH, FSH, estrogen and progesterone serum were measured. Sections of oviduct and uterus were prepared and stained by H&E method. Our findings in all experimental groups indicated a significant reduction in the body weight comparing with sham and control groups. In parameters such as uterus tissue thickness (Endometer, Miometer and Perimeter), and uterus glands, a significant decrease (p<0.05) was observed in the groups receiving 100 and 150 mg/kg doses of the extract (p<0.05). In all experimental groups, no significant changes were observed at the levels of estradiol and progesterone. FSH and LH showed a significant decrease in the groups receiving 100 and 150 mg/kg doses of the extract (p<0.05). It can be concluded that Artemisia absinthium hydroalcoholic extract in high doses has damaging effects on the reproductive system and uterine tissue, which may be due to α and β Thujoun, Alkaloid and Saponin constituents in this plant.

Inroduction & Zolpidem (with the brand name of Ambien) is a non-benzodiazepine hypnotic which binds to the benzodiazepine binding site on the GABA-A receptors given that this drug is used in more puberty so.The aim of this study was the effect of zolpidem on reproductive system of female adult NMRI mouse.
Material and In this experimental study thirty adult female mice NMRI strain at a mean weight of 30±26 grams were divided into five groups Zolpidem solution was prepared in distilled water at 5, 10, and 20 (mg/kg of body weigh) doses, and 0.5 cc injections were done intraperitoneally every day for 14 days The con trol group received no injection. The sham group received distilled water (as solvent of zolpidem) and treatment groups of 1, 2, and 3 received doses of 5, 10, and 20 mg/kg The treatment groups were sacrificed one day after the last injection, and their hearts were dissected and blood samples were obtained.The concentrations of the hormones were measured by the ELISA testThe uterus tissue was separated and examined after the process of alcohol supply, molding, shredding as well as Hematoxylin and eosin painting and the results were evaluated via the tukey-test, ANOVA by SPSS program.
The results showed a significant decrease in the mean serum, Estradiol in the experimental groups compared to the sham and control histological studies of sections showed the diameter of endometrium and uterus lumen, decreased in experimental groups compared to the sham and control Also significant decrease the number of uterus glands in experimental groups 3 compared to the sham and control.
Injection of zolpidem significantly is effective on ,uterus tissue and concentration of Estradiol hormone.

Phytotherapy is an ancient knowledge and people have been using herbs for centuries to heal themselves.Different researches have indicated that due to the side effects of synthetic drugs people nowadays have a tendency to use herbal medicines to treat their diseases.A few number of these researches have been dedicated to studying the effect of herbal medicines research. NMRI adult female mice which weighed30 to35 grams were divided into 5groups in this study.The groups included one control group, a sham group and 1, 2, and 3 experimental groups.The control group used the special water and food for mice which was fed to all the groups.The 1, 2 and 3 experimental groups received 135, 45, and 15 milligrams per kilogram of their body weight doses of the essence of Juniperus sabina respectively for a period of 30 days. The results indicate that the essence of this herb increases the weight of the uterus.All three layers showed increase thickness of diameter and the number of the blood vessels of the uterus decreased in the experimental groups 1 and 3.The number of the follicles which had undergone atrophy and the yellow-bodies (corpus luteum) increased significantly but the primordial and primary follicles decreased in number.These results suggest that this herb is harmful to pregnant women and it must be consumed under the supervision of a physician if necessary.The manner in which this herb effects the oogenesis hormones and the extraction of the effective substance and applying it on the uterus and the ovaries must be examined.

Our previous research confirmed the effects of dill seed aqueous extract on sex hormones therefore at the present study, histological changes of uterus and ovaries are compared in rats treated with dill seed and leaf aqueous extracts that use widely as food. 21 female Wistar rats were divided into three groups of control, dill seed and leaf aqueous extracts treated groups (n=7). Experimental groups were received 1ml of 0.5g/kg of extract for 25 days. At the end of experiment, blood sample were taken from dorsal aorta and estrogen, progesterone, LH and FSH concentrations were measured. Uterus and ovaries were removed and their tissue sections were stained with haematoxylin and eosin. Histomorphometrical changes in uterus and ovary were measured by ocular micrometer. Progesterone level, uterine epithelium, the total diameter and uterine glands lumen, the number of uterine glands and myometrial thickness increased only in rats treated with dill seed aqueous extract compared to the control group. Corpus luteum granulosa cells diameters and their nuclear diameter increased significantly in both experimental groups compared to the control group. Granulosa cells elongation represented their higher production of steroid hormones. Vice versa to the dill leaf aqueous extract, dill seed aqueous extract caused uterine growth. Therefore, dill seed affected reproductive system of female rats but dill leaf had less effects.

Diabetes as a metabolic disorder affects different tissues and organs، among them the female reproductive tract such as uterus may be a vulnerable organ. This study was undertaken to study the effects of hyperglycemia and insulin therapy on uterus structure، estrous cycle and serum levels of pituitary and ovarian hormones in Wistar rats. Twenty-four female Wistar rats (180-200g) were randomly divided in: control، sham (Citrate buffer i. p)، hyperglycemic (Streptozotocin; 60 mg/kg، i. p) and hyperglycemic+insulin (insulin 20 IU/Kg/day; s. c). At the end of experiment (36 Days) and in diestrus stage، all rats were weighted and blood samples were collected. Thereafter، their right uterus horn was taken out، weighted and then the middle third part of uterine horn was sampled and fixed (Bouein). After histological preparation، the histological and histomorphometrical examination of uterus was performed. Although in comparison with control and hyperglycemic+insulin groups، in hyperglycemic rats body weight، uterus weight and volume، endometrium and myometrium volume were dramatically decreased (p<0. 001) and the serum level of progesterone significantly increased (p<0. 001)، vascular dilation and infiltrating cells resembling plasma cell was also observed. No significant difference was seen between control and insulin treated group for the above parameters. Insulin deficiency، oxidative stress and alteration in the HPG axis which occurs in hyperglycemic condition، may affect the uterus structure. The observed structural changes might well play a significant role in the reproductive difficulties observed during hyperglycemia