جستجوی مقالات مرتبط با کلیدواژه « مامیران » در نشریات گروه « زیست شناسی »

Celandine (Chelidonium majus L.) is a medicinal plant belonging to the Papaveraceae and rich in isoquinolin alkaloids. Chemical synthesis of these alkaloids is not possible. So, the pharmaceutical industry requires large quantities of plants to extract these compounds. The purpose of this study was optimization of a repeatable method for regeneration of celandine by callus induction. Celandine seeds after surface sterilizing were placed on 1/2MS medium for germination. Cotyledon and hypocotyl explants were prepared from 20-day-old sterile seedlings and leaf explants from 3-month-old plants. Explants were put on Murashige and Skoog (MS) medium supplemented with different plant growth regulators (PGRs) to callus induction. The highest fresh weight was for calli in mediums containing 1 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with 0.5 or 1 mg/l Amino Purine (BAP) or Tidiazuron (TDZ). Organogenesis was observed in few treatments after repetitive subculture. Plant regeneration only happened in PGRs free MS medium, from cotyledon calli after 2 months with frequency of 11.11 % by one shoot per explant. Shoots were rooted in PGRs free MS medium with frequency of 77.78 % by 2.36 roots per explant.

Celandine (Chelidonium majus L.),a member of Papaveraceae family, is a valuable medicinal plant. Because many active substances have medicinal properties that make it great. In
order to study the effect of hormones 2,4-dichlorophenoxy acetic acid (2,4-D)
in five levels (0, 0.5, 1, 1.5 and 2 mg/l) and with hormone benzyladenine (BA) at four levels (0.25, 0.5, 1 and1.5 mg/l)
for callus, explant using stems, leaves and seeds were evaluated. To assess the
regeneration of combined hormonal BAP at three levels (1, 2 and 3 mg/l)
of 2,4-D hormone on two levels (0.1 and 0.5 mg/l) was used. This was a factorial experiment based on randomized complete design
with three replications. The results showed that the best environment for the
induction of callus induction on MS medium containing 1 mg/l 2,4-D and 0.5
mg/l BA %69.44 % rate of 2 mg/l 2,4-D and 1.5 mg/l BA %63.88 % respectively. The best medium for callus on MS medium containing 2 mg/l 2,4-D wetfresh weight. Best Celandine explants to form callus weight and stem explants. Most regeneration and leaf production in MS medium containing 2 mg/l BAP and 0.5 mg/l 2,4-D was observed but did not shoot.

In this study, the composition and biological properties of Chelidonium majus L. hydroalcoholic extract and the essential oils and also the effect of gamma irradiation on their biological properties were investigated. For this purpose, Chelidonium majus were collected through the establishment of plots from sisangan-nowshahr. Plant samples were dried at room temperature and away from sunlight for one week. Parts of samples were transferred to the Radiation Application Research Institute, irradiated at 10 and 25 kGy. Then, the essential oils extracted using Clevenger apparatus and its compounds were analyzed by GC / MS. Then, the hydroalcoholic extraction were made following estimation of flavonoid rate with standard solutions. Then, the antioxidant activities of the samples were determined by beta-carotene and DPPH tests. Based on the results of the analysis of GC / MS, 22 , 18 and 26 compounds were identified in control, 10 and 25 kGy irradiated samples, respectively. The highest flavonoid content belongs to 25 kGy irradiated samples and the lowest belongs to control samples, but these changes were not significant. Also, control and irradiated essential oils and extracts, significantly indicating strong antioxidant activities in beta-carotene and DPPH tests in compared with references. According to the results, oil and extracts derived from Chelidonium majus had certain compounds along with significant antioxidant activities. Gamma radiation had no significant effects on the compounds and antioxidant activities of plant sample.