جستجوی مقالات مرتبط با کلیدواژه « .iau science » در نشریات گروه « زیست شناسی »

High-density lipoprotein (HDL) is known to be a protective factor against coronary artery disease (CAD). In recent studies, investigating the function of HDL more than measuring its level has attracted the attention of researchers. Therefore, the aim of present study was to compare the level and function of HDL for the diagnosis of CAD.

This study (case-control) was conducted on 853 people, including 572 patients with CAD and 281 healthy subjects. Lipid profile and hs-CRP were measured by enzymatic and immunoturbidometric methods, respectively. HDL function was evaluated by measuring the activity of the paraoxonase 1 (PON1) enzyme and its effect on paraoxon substrate using the spectrophotometric method. ROC curve analysis was used to compare the detection power of the studied factors. Multivariate logistic regression analysis was used to identify risk factors that have an independent relationship with CAD.

A significant decrease in the level and function of HDL was observed in patients with CAD than in healthy individuals (p<0.001). Based on ROC curve analysis, the area under the curve (AUC) for PON1 enzyme activity was higher than HDL-C. The results of multivariable logistic regression analysis showed that diabetes, hypertension, smoking, hs-CRP, and PON1 enzyme activity are independent risk factors for CAD.

Evaluation of HDL function is a more appropriate criterion for diagnosing CAD than measuring its level. PON1 enzyme activity is suggested as a diagnostic biomarker for CAD.

The Following outbreak of the corona virus pandemic on December 12, 2019 and the declaration of a state of emergency by the World Health Organization, all researchers and researchers focused on dealing with this virus. opinion to scientists, one of the solutions to deal with the spread of the corona virus was to cut off the chain of transmission through disinfection and disinfection of high-traffic places such as airports, passenger terminals, subway stations with chemicals. Therefore, the World Health Organization presented the disinfection of high-traffic places and urban gathering points in its guidelines. The purpose of this research project is to study the disinfection power of chlorine dioxide solution in the concentrations provided by the manufacturer on spore-bearing and non-spore-bearing bacteria in order to introduce a safe and efficient disinfectant to be used against the corona virus.

In this study, which is descriptive, the disinfection power of chlorine dioxide solution on Escherichia coli and Klebsiella pneumoniae bacteria at concentrations of 1.100, 1.400, 1.800 and 1.1000 in 10 and 20 and 30 minutes were studied in three culture mediums: Blood agar, MHA and EMB. Also, the disinfecting power of the mentioned solution on Bacillus subtilis spore bacteria in concentrations of 1.400, 1.500, 1.600 and 1.700 for a period of 30 minutes and in tryptic broth medium was investigated. The results were analyzed by SPSS version 21 software.

Based on the results, the chlorine dioxide solution in all the desired dilutions destroys Escherichia coli and Klebsiella pneumoniae bacteria in 20 minutes. Also, chlorine dioxide only at a dilution of 1.400 and a duration of 30 minutes caused the destruction of Bacillus subtilis spore bacterium.

In this study, vital information was provided in the field of effective disinfection strategy of chlorine dioxide against spore-forming and non-spore-forming bacteria with different dilutions, which can be used in different dimensions for disinfection of high-traffic urban areas.

Diabetes Mellitus is related to biochemical and physiological changes in the body. In this study, the effects of anthocyanin on blood biochemical factors related to diabetes in adult diabetic rats treated with streptococci were investigated.

In this study, 32 male Wistar rats were used, which were divided into four groups as follows: a) control group b) diabetic control group that received normal saline as a solvent c) group of rats Diabetics treated with 100 mg/kg of anthocyanin d) group of healthy rats that received 100 mg/kg of anthocyanin. To induce diabetes in the experimental groups, a single dose of 50 mg/kg streptozotocin (STZ) (Sigma) dissolved in 5 mm citrate buffer (pH = 4.5) was injected intraperitoneally. Then, fasting blood sugar, insulin serum level, and lipid profile serum level were measured.

fasting blood sugar and serum levels of TG and cholesterol increased significantly in the diabetic control group compared to the control group (p<0.05). Also, fasting blood sugar levels and serum TG and cholesterol levels in the groups treated with anthocyanin showed a significant decrease in comparison with the diabetic control group (P < 0.05). In all diabetic groups compared to the control group, the level of HDL and serum insulin decreased significantly (P < 0.05), and in the groups treated with anthocyanin compared to the diabetic control group, the level of HDL and serum insulin increased. Also, the weight of rats in the diabetic control group decreased significantly, and in the treatment groups with anthocyanin, the weight of the rats increased compared to the diabetic control group (p<0.05).

Anthocyanin can be effective on fasting blood sugar, serum level of insulin, serum level of fat profile and prevent biochemical damage caused by diabetes in diabetic rats treated with streptozocin.

In many countries, including Iran, cutaneous leishmaniasis is a common and increasing disease. Proper and quick treatment of many of these patients is very difficult due to insufficient familiarity with the factors that play a role in the pathogenesis of cutaneous leishmaniasis. By using high-performance RNA sequencing methods and investigating the expression of different genes in different tissues, a new horizon has been opened on the identification of disease pathogenesis factors and obtaining new treatment methods.

This investigation was divided into two phases. Data from the NCBI website, "GSE127831" were used for the bioinformatics analysis. We conducted investigations of Gene Ontology and Differential Gene Expression. In the clinical investigation, we used qPCR to assess the expression of the genes identified in the previous stage in 27 skin samples taken from wounds caused by cutaneous leishmaniasis and 12 healthy skin samples.

According to our bioinformatics investigations, the first 100 genes with the highest drop in expression in leishmaniasis wounds are mostly involved in the production of different keratin proteins. They are mostly involved in "Intermediate Filaments Organization". The qPCR test showed that the expression of KRTAP11-1, KRT33B, KRT85, KRT35, KRT86, and KRT81 genes was greatly reduced. It shows the coordination of the results of bioinformatics and laboratory stages.

The downregulation of a specific set of genes involved in keratin formation is one of the key factors contributing to the poor healing of wounds in cutaneous leishmaniasis. keratin is a crucial chemical in developing and maintaining epithelial tissue and skin.

Diabetes is a chronic disease that affects the metabolism of sugar or blood glucose. This disease is caused by insulin resistance in the body. Diabetes can lead to various complications in the body, including liver damage. Berberine reduces the amount of damage caused by oxidative stress on mitochondria and increases the antioxidant capacity of liver tissue. exercise training causes mitochondrial biogenesis in diabetic patients. Therefore, the aim of this study was to investigate the effect of aerobic exercise with different doses of berberine chloride on the apoptosis of liver cells in diabetic male rats with streptozotocin.

In this experimental study, 64 male Wistar rats were randomly divided into 8 groups of 8. The groups include the control group, diabetic, diabetic and taking berberine chloride with a dose of 15 mg/kg, diabetic and taking berberine chloride with a dose of 30 mg/kg, diabetic with aerobic exercise, taking berberine chloride with a dose of 15 mg/kg, diabetic with aerobic exercise and the consumption of berberine chloride with a dose of 30 mg/kg, diabetics with aerobic exercise and the control group with aerobic exercise were divided. Intraperitoneal injection of streptozotocin 60mg/kg was used to make diabetic groups diabetic. Berberine chloride (15 and 30 mg/Kg) was taken orally by gavage once a day. The exercise groups performed aerobic exercise for 6 weeks with an intensity of 50-55% of maximum oxygen consumption. 48 hours after the last training session, the liver tissue was extracted to check the changes of caspase3 protein. Using the TUNEL method, the amount of apoptosis in the liver tissue was evaluated. Data were analyzed using one-way analysis of variance and Tukey's follow-up test.

In the present study, diabetic rats showed a significant increase in liver tissue apoptosis and caspase3 protein expression. Treatment of diabetic animals with berberine in doses of 15 and 30 mg/kg led to a significant reduction of apoptotic cells in diabetic rats. The expression of caspase3 protein in the diabetic group with aerobic exercise and berberine chloride consumption at a dose of 30 mg/kg was significantly decreased compared to the diabetic group and berberine chloride consumption at a dose of 15 mg/kg.

It seems that the treatment of diabetic rats with berberine chloride together with exercise activity was able to prevent the occurrence of apoptosis in the liver tissue and improve the liver damage caused by diabetes.

Osteoarthritis (OA) is the most common chronic joint disease in the world and the main cause of movement disorders in the elderly. Identification the precise molecular pathogenesis pathways of osteoarthritis disease, early diagnosis and effective treatment for OA is challenging because OA pathogenesis is still unclear. Therefore, one of the key and important goals of OA disease is to identify sensitive biomarkers, which is very valuable and effective for clinical applications, considering the availability of peripheral blood samples. The present study aimed to find differential expression genes in peripheral blood mononuclear cells (PBMCs) of OA patients and introduced a reliable biomarker to distinguish OA via bioinformatics analysis.

The microarray dataset of PBMCs of OA patients and normal individuals (GSE48556) was obtained from the GEO database. Differential expression analysis between OA and normal groups was performed using GEO2R and genes with differential expression were isolated. Signaling pathways and GO analysis were determined using Enrichr databases. Next, genes with differential expression were introduced and ROC curve analysis was performed for analysis the probability of the selected gene as a biomarker.

The bioinformatics results showed 1515 genes had significant differential expression which 657 genes upregulated and 858 gens downregulated. Analysis of signaling pathways showed that proteasome pathways, chemokine signaling pathway and FoxO signaling pathway are important in this disease. It was also found that the SRSF10 gene has the most downregulation in OA patients and the ROC curve analysis showed that this gene can differentiate OA patients from healthy individuals with high sensitivity and specificity.

Overall, our data demonstrated that the differential genes expressions have potentially to be considered as biological biomarkers with diagnostic and therapeutic target approaches. In this regard, the reduction of SRSF10 gene expression can probably be a biomarker to identify OA disease in PBMC cells, which should be confirmed in experimental analysis.

Successful delivery of a gene to a target cell requires the development of a safer and more efficient gene transfer system. This study aimed to evaluate the transfection efficiency of cationic virosome derived from vesicular stomatitis virus (VSV) as a hybrid nano-carrier compared to two commercial transfection reagents including Lipofectamine™ 2000 and, GenJet™ in HEK-293 and, Vero cell lines.

The transfection efficiency of VSV cationic virosome, Lipofectamine™ 2000, and GenJet™ was evaluated in HEK-293 and Vero cell lines using pEGFP-N1 plasmid transfection, fluorescent microscopy, and flow cytometry methods. Also, the toxicity of cationic virosome and commercial transfection reagents was investigated by the MTT test. The inhibitory effect of NH4Cl on virosome transfection efficiency was analyzed by fluorescent microscopy and flow cytometry.

Our findings showed that the highest level of EGFP expression was obtained in cells transfected with Lipofectamine™ 2000. Transfection efficiency was significantly increased in both cells transfected with virosome/pEGFP-N1 compared to pEGFP-N1/GenJet™ (P< 0.05, P< 0.01). In addition, the results of cell viability analysis by MTT were consistent with the results obtained from the evaluation of transfection efficiency. Cells transfected with lipofectamine™ 2000 showed the highest survival rate. Cell viability was significantly higher in cells transfected with VSV cationic virosomes compared to cells transfected with GenJet™ (P< 0.05). The results showed that transfection was inhibited in HEK-293 cells treated with NH4Cl.

VSV-derived cationic virosome containing VSV-G is an efficient hybrid nanocarrier with broad cellular tropism, which provides new insight into its application as a gene delivery system in gene therapy studies.

Nanocomposites are prepared from various materials that may have antibacterial activity. This study aimed to investigate antibacterial and antifungal activityies of nanocomposites of bohemite-gold and bohemite-gold-chitosan by help of aqeous extract of peppermint.

In the current study, the nanocomposites were prepared by exextracellular biosynthesis and antibacterial properties were investigated by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and killing-time curve by classic method of consecutive dilutions by Clinical & Laboratory Standards Institute.

The results showed that MIC and MBC were 10 µg/mL and 20 µg/mL for bohemite group, respectively. The results also showed that MIC and MBC were 2.50 µg/mL and 5 µg/mL against Acinetobacter baumannii and 5 µg/mL and 10 µg/mL against Escherichia coli, respectively. The greatest antibacterial activity for of bohemite-gold and bohemite-gold-chitosan was observed for a period of 6 to 24 h. Based on ourfindings, bohemite showed lower antibacterial activity compared with bohemite-gold and bohemite-gold-chitosan against the mentioned bacteria.

The prepared nanocomposites did not show any antifungal activity. Thus, bohemite-gold and bohemite-gold-chitosan nanocomposites have antibacterial activities against bacteria inducing infection in wound.

Toxic metals such as cadmium and the oxidative stress caused by them can develop many Disease such as atherosclerosis. Atherosclerosis is a progressive chronic inflammatory disease that leads to the coronary ischemic with the accumulation of fats at the branching point of the vessels. Dysfunction of many genes, such as connexin, which play a role in lipid homeostasis, can cause atherosclerosis. The present study investigates the effect of cadmium on the expression level of connexin 43 gene in RAW264-7 cell line.

In this study, RAW264-7 cell line was cultured and affected by different doses of cadmium chloride. IC50 was determined using MTT test, RNA was extracted and connexin 43 gene expression was evaluated by Real Time PCR method. The results were analyzed using SPSS software with oneway ANOVA statistical method.

Our findings showed that the expression of connexin 43 gene in polluted cells by 6.3 μM cadmium had a significant increase compared with control group, especially in a short time. Also, the IC50 results indicate a decrease in cell viability in this concentration.

It seems that cadmium in moderate concentrations and in a short time can exert cytotoxic effects on the scavenger receptors of macrophage cells by increasing the expression of connexin 43 gene in RAW264-7 cell line and lead to the development of atherosclerosis.

Plant bioactive substances are a valuable source of various medicines that play an important role in human health. Increasing resistance to different antibiotics has increased the importance of using plant extracts as they have potential sources of antioxidants. Antioxidant compounds of plant origin can protect the body from damage caused by oxidative stress. Recently, the use of natural antioxidants derived from medicinal plants has had medicinal and medical applications. Comparison of the antioxidant activity and total phenolic compounds of Thuja orientalis leaf extracts isolated from the three heights (1, 2, and 3 meters) of Noshahr and Tehran.

In this experimental study, the leaves of Thuja orientalis were collected in spring from Tehran and Noshahr cities at three heights (1, 2, and 3 meters). Subsequently, the methanolic and aqueous extracts were prepared and their antioxidant activities were evaluated using DPPH and FRAP methods. In addition, the content of the phenolic extract of the extracts was measured by the Folin-Succtile method.

The results of this study showed that both aqueous and the methanolic extracts of T. orientalis exhibited significant total antioxidant activities at a height of 3 meteres. The methanolic extract of T. orientalis collected from Tehran and Noshahr in height of 2 meters showed the howest amounts of antioxidant activity. Moreover, the highest and lowest amounts of total phenolic compounds were belonged to aqueous and the methanolic extracts of T. orientalis collected from Noshahr.  

The results of this study showed that the leaf extract of the C. orientalis can be used as an promising source for the synthesis of antioxidant compounds.

Breast cancer remained the leading cause of cancer-related death among women worldwide. Despite considerable advances in early detection and effective therapy, developing chemoresistance and metastatic spread to distant organs is still a pressing clinical challenge. The present study aims to investigate the role of Aurora-B as a potential oncogene in the progression of breast cancer.

Forty breast tumor samples and paired adjacent non-tumor tissues were collected. RNA was extracted and converted to cDNA according to manufacture protocols. The expression of Aurora-B was assessed using quantitative real-time PCR. The relationship between gene expression and the pathological features of the tumors, and the hormone receptors status of the patient’s tumors were also analyzed.

We found a significant overexpression of the Aurora-B gene in tumor samples compared to adjacent normal tissue (p value < 0.05). Moreover, the overexpression of Aurora-B was more increased in IDC tumors in compare to ILC breast tumors. There was not significant change between pre- and post-menopause periods.

Overexpression of oncogenic Aurora-B is associated with the progression of breast cancer. Aurora-B may represent an emerging therapeutic target in breast cancer treatment.

The genus Lepidium L. is one of the three large genera of the Brassicaceae family, which are used as vegetables or medicinal plants. Knowing the relationships and genetic diversity of Lepidium L. species by molecular markers is an ideal approach to improve and conserve of their germplasm.

The genetic diversity and relationships among 22 populations belonging to three species of L. sativum, L. draba and L. latifolium using combined data of SSR, ISSR and SCoT markers based on analysis of molecular variance, indices of genetic diversity, cluster analysis, principal coordinates analysis and genetic structure were investigated.

Analysis of molecular variance showed that the genetic diversity within the groups is more than between the groups. The highest and lowest genetic diversity was related to L. sativum and L. latifolium, respectively. The highest Nei genetic similarity matrix was observed between L. draba and L. latifolium and the lowest similarity between L. sativum and L. latifolium. Neighbor-Joining cluster analysis divided the Lepidium populations into three groups and there was a weak separation between the species, which was confirmed by principal coordinate’s analysis and genetic structure, and it seems to be due to the gene flow among the populations of different Lepidium species and the existence of a common gene pool between them.

The results showed that the populations of the three studied Lepidium species have a high level of genetic diversity and gene flow, which can be used as valuable genetic resources in breeding programs.

The promotion of a high level of food safety is a major policy priority worldwide. Food safety is an inherent concept of food safety and is related to many aspects of agricultural technologies as well as food production and processing. Foodborne diseases are among the most serious public health problems worldwide and are a major cause of morbidity. Polymerase chain reaction (PCR) based methods, both qualitative and quantitative, are also used for more highly processed soy-based food products PCR methods have been proven to be the most sensitive and reliable means of detecting and quantifying genetically engineered traits in crops and foods.

In this study, we have used 20 distinctive sausages produced in Tehran, and also raw meat (Cattle, sheep, and poultry) and soy protein were used as control samples. DNA was extracted from all collected and control samples, and then the quality and quantity of them were analyzed by electrophoresis and photometry respectively. To confirm the ingredients listed on the sample labels, PCR was used with specific primers for each species.

Fragments of 118 bp were amplified for the soybean lectin gene, 183 bp for the poultry 12S rRNA gene, 273 bp and 336 bp for the cattle and sheep cytochrome b genes, respectively. The PCR results indicated that chicken and soy were present in 90% of the cases, contrary to the labels on the product page.

Thus PCR has advantages over other common methods such as high specificity and sensitivity, repeatability, and rapid analysis of fraud in meat products.

Biomedical science has expanded its field of data processing even more and now studies the most complex product of evolution, the human brain. In this article, modeling of the emerging knowledge of neuroinformatics from bioinformatics has been discussed. After an overview of bioinformatics, history, different areas of neuroinformatics and the complexity and heterogeneity of its data are examined, and finally, methodological limitations in the field of brain research are pointed out.

Breast cancer is the most common cancer in women, which is the main cause of cancer-related death in women. The use of herbal compounds as anti-tumor with less side effects than chemotherapy has been considered. But it seems that over time drug resistance will reach this type of material. This study has been conducted to determine the effect of plant extracts and exosomes extracted from their effects on MCF7 cancer cells.

  In this comparative study, MCF7 cells were cultured in medium and treated with different concentrations of oleuropein, quercetin, coumarin and Valproic acid for 24 hours. Then the cell viability was evaluated by MTT method and its IC50 values were calculated and exosome was extracted from the culture medium of the treatments and applied to MCF7 and their IC50 values were also calculated by MTT method.

The results of MTT confirmed the inhibitory properties of four natural substances and their combination on MCf7 cells. Coumarin and quercetin were the most effective treatments of the studied materials. But the exosome obtained by applying 50% of their concentration had an inhibitory role with higher concentrations and the exosomes obtained from their IC75 actions had played their role in lower inhibitory concentrations.

Inhibition of breast cancer cells by the exosomes obtained from the combination of the above-mentioned 4 substances occurred in a lower dose Because at this concentration, all cell death-inducing substances and their signals accumulate in the exosome, and by transferring into cancer cells, they induce cell death.

Farnesol and gingerol are two powerful plant active ingredients that have proven anti-cancer and antioxidant effects. In this study, the expression changes of Caspase 8, Matrix metalloproteinases 2, Matrix metalloproteinases 9 and Vascular endothelial growth factor genes were studied in MCF-7 and SKBR3 cancer cell lines treated with niosomes containing farnesol and gingerol.

MCF-7 and SKBR3 cells were treated with niosome containing farnesol and gingerol, combination of two drugs, single drug niosome formulation and free drug for 48 hours. Gene expression changes were investigated using Real-Time PCR technique and statistical analysis was done at 0.05 level with GraphPad Prism software.

Niosome codelivery containing two drugs (N-Far/Gin) caused a significant increase in the level of caspase 8 gene expression and also a significant decrease in the regulation of the expression of Matrix metalloproteinases 2, Matrix metalloproteinases 9, and Vascular endothelial growth factor genes (P<0/001). It had a higher significance level than the treatment with other formulations.

It seems that farnesol and gingerol-loaded niosomes have great anti-apoptotic, anti-metastatic, and anti-angiogenic potential in the prevention of breast cancer. Further in vivo experiments and clinical studies can elucidate the potential of N-Far/Gin to be applied as a medicinal agent in breast cancer treatment.

Histones are replaced by protamines to package sperm head DNA during mammalian spermatogenesis. Protamine genes variation cause sperm DNA damage and is affect infertility in men. Therefore, this study aim was investigation on association of two rs737008 in PRM1 gene and rs4780356 in PRM2 gene polymorphisms with azoospermia and oligospermia in Iranian idiopathic infertile men.

DNA extraction from blood samples of 96 idiopathic infertile men with azoospermia and oligospermia and 100 normal control men. Prevalance of two studied polymorphisms was identified by restriction fragment length polymorphism (PCR-RFLP). Then results were confirmed with sequencing.

A mutant allele frequency of rs737008 in PRM1 gene polymorphism was difference in patient and control groups but statistical analysis showed no significant association between this polymorphism prevalance among case and control groups (P>0.05). By genotyping shown no existent rs4780356 in PRM2 gene polymorphism in anyone of case and control groups.

This study finding indicated there was not association with prevalence of two rs737008 in PRM1 gene and rs4780356 in PRM2 gene polymorphisms with oligospermia and azospermia and idiopatic male infertility in Iranian population. More studies need to demonstrate the role of these two polymorphisms with idiopathic infertility in Iranian men.

Plants are a rich source of bioactive chemical compounds that play an important role in the prevention and treatment of infectious diseases. In this study, the ability of aqueous and ethanolic extracts of sumac (Rhus coriaria) fruit to inhibit the growth of Trichomonas vaginalis and Candida albicans were investigated in vitro.

Extraction of sumac fruit was done by soaking and stirring with water and ethanol and its antimicrobial effects were evaluated by Broth microdilution method by determining the minimum inhibitory concentration (MIC).

The 50% inhibitory concentration (IC50) of ethanolic extract against Trichomonas vaginalis was 1.8 mg/ml and for aqueous extract was 3.55 mg/ml in 24 hours. No concentration of the aqueous extract of Sumac fruit was able to inhibit the growth of Candida albicans, while the ethanolic extract at 60 mg/ml inhibited the growth of Candida albicans after 24 hours.

Sumac fruit extracts can be considered as a potential alternative to chemical drugs in the treatment of Trichomonas vaginalis infections, but these compounds have not shown favorable antifungal results against Candida albicans.

Breast cancer is the most common type of cancer among women, which occurs in the epithelial tissue of the mammary gland. Various environmental, genetic and epigenetic factors play a role in the occurrence of breast cancer. According to the previous studies, one of the most important epigenetic changes involved in the occurrence of breast cancer is the dysregulationof the expression level of microRNAs. Therefore, the aim of this study was to investigate the expression level of miR-509-3-5p and miR-596 in breast cancer tumor tissue.

In the bioinformatics analysis, the datasets related to miRNAs (GSE40525 and GSE45666) were prepared from the GEO database. Data analysis was performed using the Affy package in R software. Sampling was done from 100 women with breast cancer and 100 control. Tissue RNA was extracted using Trizol solution. Then, using the cDNA synthesis kit, DNA was synthesized from the extracted RNAs, and the expression level of miR-509-3-5p and miR-596 was investigated using Realtime PCR method.

According to the bioinformatics results, the expression level of miR-509-3-5p and miR-596 in breast cancer tissue reduced compared to healthy tissue. The expression level of miR-509-3-5p and miR-596 in tumor tissue was significantly lower than normal tissue (p<0.05).So these results confirmed the results of bioinformatics studies.

According to the results of the present study, which showed a decrease in the expression of miR-509-3-5p and miR-596 in breast cancer, it can be said that these miRNAs can be used as important diagnostic and therapeutic biomarkers in breast cancer.

Oncolytic viral therapy is known as promising approach for cancer treatment. Oncolytic viruses like adenoviruses can proliferate in cancerous cell and disintegrated these cells.  Serotype 5 adenovirus attached on surface of host cell via coxsackie-adenovirus receptors. The expression rate of this receptor is highly variable in surface of cancerous cells. The aim of the present study was production of an oncolytic adenoviruses with high selectivity feature for gastric adenocarcinoma cells (AGS) that can proliferate in this cell and destroy them.

In this experimental study, the octreotate sequence was cloned into HI-loop of the adenovirus fiber. Thus, the pAd5TATE (wt) and pAd5TATE (ΔE1, ΔE3) recombinant vectors were constructed. pAdZ-TATE (wt) vector was synthesized using pADZ cloning system. Ad5TATE (wt) and Ad5TATE (ΔE1, ΔE3) recombinant viral particles were synthesized and propagated in HEK293 cells. Using the calcium phosphate procedure, the HEK293 cell line was transfected with the recombinant vectors and the new virus particles were produced. The AGS cell line was transfected by the Ad5 (wt) and Ad5TATE (wt) and the cell destruction of these cells were investigated in different times. Using the MTT test the cell surveillance rate was evaluated in AGS cell line that treated with recombinant vector. Cell destruction effects (CPE) started in less than 18 hours and were completed 72 hours after infection.

The titer of recombinant viruses was calculated by the TCID50 method as 107 (for 100 Lµ of viral solution). Also, Ad5TATE (ΔE1, ΔE3) virus titer was 107 TCID50/ml by the TCID50 method. The results showed that Ad5TATE (wt) could infect AGS cells compared to Ad5(wt). The results of the MTT test showed that with the increase in the concentration of viral particles and storage time, the number of live AGS cells decreased significantly.

The findings of this research showed that the Ad5TATE (wt) recombinant virus could specifically infect gastric adenocarcinoma (AGS) cancer cells and destroy them after replication. Therefore, the Ad5TATE (wt) virus can be used as an oncolytic agent against gastric adenocarcinoma (AGS) cancer cells.