جستجوی مقالات مرتبط با کلیدواژه « endometrial stem cells » در نشریات گروه « زیست شناسی »

Diabetes Mellitus is an autoimmune and chronic disorder that has spread rapidly all over the world due to lifestyle and obesity. In this study, we are trying to find an effective step in controlling diabetes by designing an engineered tissue and grafting it to different sites in the animal model. Uterine endometrial mesenchymal stem cells (EnMSCs) were prepared using the enzymatic extraction method and PAN nanofiber scaffold by electrospinning method. EnMSCs were cultured on the scaffold and transplanted into diabetic rats treated with streptozotocin. The engineered tissue was transplanted in one group peritoneally in the abdominal cavity and the other group subcutaneously. Also, in another group of rats, EnMSCs were injected through the tail. After transplantation, blood glucose, insulin, and weight of rats were measured. The findings of the present study showed that the method and area of stem cell transplantation play an important role in the control of diabetes. In the groups receiving EnMSCs, glucose concentration, blood insulin level, and body weight were improved compared to the control group. Compared to other groups, glucose concentration decreased significantly and blood insulin level and body weight increased significantly in rats receiving a peritoneal transplant. In the subcutaneous transplant group and the injection group, there was no significant difference in the investigated criteria. According to the results of this study, transplantation of EnMSCs using PAN scaffold in the peritoneal site can be suggested for the treatment of diabetes, although more studies are needed in this field to provide a complete treatment.

Parkinson’s disease is the second most common age-related neurodegenerative disease with no identified effective treatment. The aim of this study was to evaluate the effects of human endometrial stem cells with the anti-inflammatory drug pomalidomide on glutathione (GSH) and serum lipids (total cholesterol,TC and low density lipoprotein, LDL)) in the Parkinson’s model in male Wistar rats. Male rats were randomly assigned to five groups (8 animals in each group) including: control, Parkinson’s, and 3 experimental Parkinson’s groups receiving stem cells, pomalidomide, and a combination of stem cells and pomalidomide. Parkinson’s was induced by injection of 6-hydroxy dopamine (concentration 6 μg) in the striatum by stereotaxic method. In the fourth week after surgery, the three experimental groups were treated with  4 mg/kg/daily of pomalidomide, 100,000  stem cells through intranasal route, and a combination of pomalidomide at a dose of 4 mg/kg/daily and 100,000 stem cells. At the end of day 28, blood samples were taken from the groups and factors were measured. The results revealed a significant increase in glutathione among the treated groups compared to the Parkinson’s group. There was also a significant decrease in glutathione in Parkinson’s group compared to the control group. Comparison between Parkinson’s group and the group treated with pomalidomide and endometrial stem cells showed a significant reduction in cholesterol. Moreover, LDL levels showed a significant decrease in LDL levels in the treated groups compared to the Parkinson’s group. Considering the improvement of the measured factors in Parkinson’s rat, allogeneic stem cells can be used as a potential source along with pomalidomide in future research for the treatment of Parkinson’s disease.

Prostate cancer is the second leading cause of cancer deaths in men. Androgen ‎deprivation therapy has been identified to induce oxidative stress in prostate cancer, ‎leading to reactivation of androgen receptor (AR) signaling. Thus, antioxidant therapies ‎have gained attention as adjuvants for this cancer. Here, we report for the first time that ‎endostatin along with endometrial stem cells expressing TSP-1 anti-angiogenic gene ‎improved androgen- phenotype and genotype in rats prostate cancer cells.‎‏ ‏Forty male ‎rats (n=8) weighing approximately 95-100 g were purchased from Pasteur Inistitute of ‎Tehran . Animals were suffered to prostate cancer by injecting LNCaP cell line for 12 ‎weeks. Prostate cancer animals were immediately received endostatin for 28 days. ‎Prostate cancer animals were received stem cell containing the Tsp-1 anti-angiogenesis ‎gene. Histological examinations were measured. The levels of IL8 and VEGF serum ‎were assayed.‎‏ ‏In group receiving cells and drug, amount and height of acinus wall folds ‎decreased compared to the patient group and the shape of the vesicles was clear and the ‎epithelial lining were cohesive and regular. The levels of IL8 and VEGF serum were ‎significantly decreased in combination group than to prostate cancer group (P≤0.01). ‎

The engineered tissue structure includes three components: cells, signaling pathway and scaffold. The polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties because of using PLGA polymer and hydroxyapatite. The aim of this study was to evaluate the biocompatibility effect of PLGA/HA scafffold on osteoblast differentiated from hEnSCs. We aimed to evallute the adhesin of cells on scaffolds. hEnSCs were isolated from human endometrium tissue and induced the osteogenic differentiation, then the differentiated cells were cultured on PLGA/HA synthetic scaffolds. A nanocomposite scaffolds based on PLGA/HA were fabricated by electrospinning methode and behavior of differentiated osteoblast cells was evaluated after seeding cells on this scaffold. Osteogenesis was investigated in terms of attachment, alkaline phosphatase activity and gene expression. Morphogenic of cells in scaffold was evaluated by SEM and biocampatibility of this scaffold was confirmed by MTT assay. The alkaline phosphatase activity confirmed osteoblast differentiation. SEM studies showed that the surface properties of scaffold were desirable and the cells had the ability to attach and proliferate better on the nanocomposite scaffolds. It has been shown that the nanocomposite scaffolds have appropriate properties to support the attachment of differentiated osteoblast cells.

Human endometrial stem cells (hEnSCs) contain a cell population that are capable of self-renewal and show similar properties to mesenchymal stem cells. The aim of this research is to show that these cells can differentiate to different cell lineages. In this study, endometrial tissue obtained from patients cells were extracted enzymaticaly. The released cells were cultured in DMEM/F12 with 10% FBS. The flow cytometry analysis was done for CD105, CD90, CD146, CD31 and CD34 in the third passage. The adipogenic, osteogenic, neurogenic and endothelial differentiation was evaluated in the third passage after 21 days of induction with differentiation media. The flow cytometry analysis showed that EnSCs were positive for CD90, CD105 and CD146 and were negative for CD31 and CD34. Moreover, results showed that the hEnSCs can differentiate to osteocyte and adipocyte. This was confirmed by alizarin Red and oil-Red O staining. Immunocytochemistry assey show that the markers including Nestin, MAP2 and CD31 has been expressede in differentiated cells into neuron and endothelial. Results showed that hEnSCs appear to be able to provide a source of different kinds of cells that will be able to be used to further study the cellular differentiation processes and cell therapy.