جستجوی مقالات مرتبط با کلیدواژه "sperm quality" در نشریات گروه "زیست شناسی"

Approximately half of infertility in couples can be attributed to male factors, which may occur in a wide range of causes. Examining seminal plasma parameters is the initial and simplest approach in male infertility evaluation and provides valuable information about fertility status. In this study, the presence and relationship between the concentration of interleukin 35 (IL-35) in seminal plasma and standard parameters of sperm in infertile men were investigated. Semen analysis was performed on semen samples of men referred to the Infertility Center of Shiraz University of Medical Sciences. Semen samples were analyzed in accordance with the laboratory guidelines of the World Health Organization and were divided into three groups including 21 normozoospermic, 21 asthenoteratozoospermic, and 15 azoospermic samples. The seminal concentration level of IL-35 was analyzed using the enzyme-linked immunosorbent assay (ELISA) method. Results showed that IL-35 was detectable with different levels in the seminal plasma of studied groups, however, its concentration did not show a statistically significant difference (p>0.05). The correlation analysis between IL-35 concentration and sperm standard parameters showed that there is a significant negative correlation between IL-35 concentration and the normal sperm morphology percentage (r=0.317, p=0.04), while no significant correlation was observed with sperm count (r=-0.27, p=0.08) and sperm motility (r=-0.27, p=0.08). Despite the presence of IL-35 in semen, even in normozoospermic male patients, its role and functional significance have not been determined. The existence of a negative relationship between the IL-35 concentration and the normal sperm morphology percentage can indicate its importance in male infertility status.

The aim of this study was to investigate the effect of different levels of peppermint extract on semen quality of Arabi rams. Semen was collected from 12 Arabi rams weekly for 8 weeks and immediately mixed, and then were divided into 5 parts and received zero, 50, 100, 150 and 200 μl/ml of peppermint extract. At different storage times of diluted semen containing treatments (zero, 24, 48 and 72 hours) in liquid condition, semen quality parameters were evaluated. Immediately after sperm collection, the lowest total motility and sperm viability belonged to the level of 200 peppermint extract (P<0.05). At 24 hours, levels 150 and 200 of extract increased the rate of morphological abnormalities of sperms. At this time, the lowest level of sperm plasma membrane integrity was related to the level of 200 peppermint extracts (P<0.05). 48 hours after sperm storage, the highest total motility, viability and plasma membrane integrity of sperms were belonged to the control group (P<0.05). 72 hours after semen storage, no statistically significant differences were found among treatments for all sperm quality parameters. At this time, the highest concentration of malondialdehyde (MDA) in seminal plasma was related to the level of 100 μl/ml peppermint extract (P<0.05). In general, by adding different levels of peppermint extract to the diluent and maintaining the diluted semen of the Arabi ram in a liquid state at 5°C, the concentrations used of peppermint extract not only did not improve the qualitative parameters of the sperm, it also had a devastating effect.

This study aimed to investigate the relationship between the expression of the antioxidant gene of the nuclear factor Erythroid 2 related to factor 2 (Nrf2) and sperm quality in asthenoteratozoospermia men.

The study was conducted at the University Jihad Infertility Treatment Center in Qom, Iran. In this study, 50 infertile with asthenoteratozoospermia and 50 fertile individuals were enrolled as the control group. Sperm parameters were evaluated according to WHO (2010). Sperm DNA fragmentation, Nrf2 gene expression, and antioxidant enzyme levels were assessed with TUNEL, RT-PCR, and ELISA kits, respectively. The significance level was considered to be p < 0.05.

The results showed that the expression level of the NRF2 gene in patients with asthenoteratozoospermia was lower than the control group (P <0.05). The quality of sperm parameters and the level of antioxidant enzymes were lower than the control group (P <0.05). A Significant association was observed between gene expression of Nrf2 gene, sperm parameters and levels of antioxidant enzymes (p < 0.05).

Our findings show that mRNA NRF2 expression is significantly associated with the quality of sperm parameters in asthenoteratozoospermia men. This suggests that NRF2 is important for spermatogenesis and may serve as a useful indicator in the diagnosis of male infertility.

Copper sulfate is one of the most important environmental pollutants that has the ability to produce free radicals and create oxidative stress. The aim of this study was to investigate the effects of copper sulfate on sperm quality parameters, DNA fragmentation rate and testicular tissue of adult Wistar rats.

In this experimental study, 30 adult Wistar rats weighing 250-200 grams were used. Random adult mice were treated in 3 control groups, copper sulfate receptor with concentration (100 mg / kg) and concentration (200 mg / kg) for 56 days. At the end of the treatment period, testicular weight, sperm count and parameters were assessed based on (WHO2010). The quality of sperm chromatin was assessed by acridine uranium nuclear pigments. Malondialdehyde level was measured. Data were analyzed using the One-Way ANOVA statistical method.

The quality of sperm parameters in copper sulfate with a concentration of 200 mg / kg decreased significantly (P <0.05). The testicular weight was significantly reduced at a dose of 200 mg / kg (P <0.05). The diameter of the seminiferous tubules, testosterone levels,, germ cell count, and sperm DNA fragmentation rate decreased at a dose of 200 mg / kg (P <0.05). The concentration of malondialdehyde at a dose of 200 mg / kg sulfate copper was significantly increased (P <0.05).

This study shows that high concentrations of copper sulfate cause destructive effects on sperm quality, testicular tissue.

X-ray is the one of the types of ionizing electromagnetic rays that is widely used in medical and industrial professions, but also has destructive effects. The present study was conducted to determine the effect of X-ray on count and motility of sperm cells of in adult male rat. 40 male rats were divided into two groups, the treatment group with X-ray with variant radiography and control group, respectively. Then, the total number of sperms and their mobility were analyzed by T-test in both groups. The results showed that X-ray was reduced the total number of sperms significantly. The number of dead and immotile sperms increased in X-treated conditions compared to control group significantly. However, the number of active- progressive and slow motion and non- progressive sperms in the X-ray treated group was not significantly different with the control group. It is concluded that X-ray can have destructive effects on male reproductive systems, including reduction sperm count and motility.

Sperm quality evalution is a valuable tool to study the reproduction potential of male animals and is important for breeding and preservation of medical species. In this study, 66 Zanjani male snakes were collected from two mountainous and cold habitats, Bostanabad in eastern Azarbaijan and Tarom of Zanjan Province of Iran, in year 2011 to 2012. Morphological characteristics of animals, including body weight, body length from snout to vent, specifications of vas deferen duct and sperm parameters including: concentration, motility, viability of sperm and also the presence and absence of ductal sperm were examined. Results show the morphology of vas deferens in Zanjani viper consists of three parts: proximal, median and distal. The longest values of vas deferen (right and left) lengths in Bostanabad viper was observed in the autumn and the shortest lengths in the spring, whereas these values for Tarom snakes were opposite. The highest mean percentage of sperm concentration in Bostanabad samples was observed in the spring and in Tarom samples was in the autumn. According to the results we can recommend concentration of mature sperms with suitable reproductive parameter are highest in the vas deferen in May, October and November and sperms with highest motility are present in the distal part of the duct in the spring and autumn, and it seems animal is ready for mating.