جستجوی مقالات مرتبط با کلیدواژه « اپی گالوکاتچین گالات » در نشریات گروه « زیست شناسی »

Matrix metalloproteinases (MMPs) are a zinc endopeptidase family that increases the metastatic behavior of human malignant tumors. Epigallocatechin gallate (EGCG) is a major component of green tea polyphenols and is used as an MMP inhibitor in cancer treatment. This study aims to develop and optimize the loading of EGCG in the liposomal delivery system in an experimental/ computational way. In this study, nanoliposomes were prepared by passive loading and thin-film hydration method. Size, zeta potential, stability, encapsulation efficiency, and nanoliposome drug release profile were investigated. Cytotoxicity of nanoliposomes was evaluated on three breast cancer cell lines using an MTT viability assay. To investigate the EGCG-Liposome interaction, coarse-grained Molecular Dynamic simulations were carried out. The mean diameter of liposome was 73.6±6.9 nm, the surface charge was -14.6 mV and the encapsulation efficiency was 78.5±7.3%. The encapsulation of EGCG into the liposome caused a continuous release of the drug after 72 h, which also increased the potency of the drug. Due to the EGCG hydrophobic properties, the major distribution is located at the hydrophobic part of the membrane. The energy and radial distribution function results indicate the stability of liposomes. Simulation results demonstrate that the majority of the drug is surrounded by liposomes, which indicates high encapsulation efficiency and confirms the developed synthesis method. Due to the low solubility of the drug, it seems that the use of liposomal carriers to deliver and release EGCG is a suitable solution to increase the efficiency of the drug.

Due to the importance of constituent Epigallocatechin gallate (EGCG), in the tea plant in use of pharmaceutical and food industries, the amount of EGCG in young leaf, old leaf and callus extract was compared and measured. EGCG in callus extract was isolated and measured by Molecularly Imprinted Polymer (MIP) technique in 1397 in Giah Essence Phytopharm Company. In order to callus induction, callus of tea plant which was harvested from the western region of Mazandaran province cultured in WPM, SH, MS media with different amounts of plant hormones. Leaves and callus extracted with 70% methanol by maceration method. For optimization of extraction and purification, various quantities of functional multiwalled-CNTs 1-10 mg used in production of CNTs-MIP and the performance investigated. EGCG of callus extract extracted with CNTs-MIP and its amount determined with HPLC. The highest volume of callus in WPM medium ,and the highest amount of EGCG in SH medium observed with optimized concentrations of hormones BA(2 mg/l), 2,4_D(0.5 mg/l). The highest content of EGCG was extracted by adding 8 mg of MWCNTs in the polymer structure. EGCG in young and old leaves and callus were 37.34, 7.19 and 0.13 mg/g of dried weight, respectively. By CNTs-MIP, the EGCG content in callus was obtained 0.91 mg/g. Despite the trace amount of EGCG in callus, the amount of this substance can be measured with a concentration coefficient of 8 times by CNTs-MIP, demonstrated the efficiency of this technique.