جستجوی مقالات مرتبط با کلیدواژه « Hematology » در نشریات گروه « زیست شناسی »

In recent years, with the growing growth of chemicals based on nanotechnology, on the one hand, and on the other hand, special capabilities such as photolysis in nanomaterials, which will cause the destruction and elimination of organic pollutants, we have witnessed the entry of these materials into the environment. Different waters are like wetlands. In this research, considering the widespread use of titanium dioxide nanomaterials to remove organic sediments of Anzali lagoon in the past years, by creating the simulated conditions of that lagoon on a laboratory scale, the hematological test of the carp species (Ctenopharyngodon Idella),in contact with Titanium dioxide nanoparticles were used to investigate the toxicological effects of this substance in the aquatic environment.

The average lethal concentration LC50 for Amur species was calculated and the daily mortality rate was determined and recorded by Spss analysis. The fish were chronically exposed to TiO2 nanoparticles for 2 weeks in the treatments of 0.1, 0.1, 1, and 10 ppm. After 14 days, blood samples were taken from the fish and blood samples were analyzed to analyze the blood parameters.

  By examining the blood parameters of RBC, Hb, HCT, MCV, WBC, MCH and MCHC, there were significant differences, including a decrease in the first three cases (P<0.05) and an increase in the last three cases (P>0 /05), was observed among different treatments and the control group.The lethal concentration (LC50) for Amur species was also 12834 ppm.

Nanomaterials of titanium dioxide in the examined concentrations have a negative effect on the hematological factors of Amur fish and cause a decrease in red blood cells, hemoglobin and hematocrit in the blood of Amur fish, which ultimately leads to the death of this species of fish. Therefore, this fish can be a suitable indicator for the toxicology evaluation of titanium dioxide nanoparticles.

In the past few years, the use of safe stimuli of vegetable origin has increased in aquaculture. Ginger is one of the medicinal plants that has been used in aquaculture in a number of studies. Ginger has attracted much attention due to its important effects on growth and health

In the present study, changes in hematological and some blood and biochemical parameters of rainbow trout with mean weight of 45.80±3.60 g by adding 0, 1, 3, 6 and 10 g / kg ginger extract in the diet for 8 weeks were evaluated.

The results showed that the use of ginger extract in the diet of rats, white blood cells, hematocrit and lymphocytes in the 10 g / kg treatment had a significant difference with the control group (P <0.05), while hemoglobin, MCV, MCH, MCHC, neutrophil counts and monocytes showed no significant difference (P> 0.05). Total protein, total albumin, and total globulin also showed a significant difference with the control group (P <0.05), but glucose, cholesterol and triglyceride did not significantly (P> 0.05).

The results of the present study showed that the use of ginger extract in the diet changes some blood and biochemical parameters of rainbow trout blood and improves the health of rainbow trout.

This study was conducted to investigate the effects of different levels of withania coagulans water extract (0, 250, 500, and 1000 mg/kg diet, in 3 repetitions) on growth, liver enzymes activity, blood parameters including WBC, RBC, Hct, Hb and non-specific immunity parameters (IgM and lysozyme) of common carp. The fish were fed for 8 weeks. The results showed that growth performance in treatments containing the extract increased significantly compared to the fish in the control (p<0.05). The activity of hepatic enzymes (ALT, AST, ALP, and LDH) showed a significant difference in fish-fed extract in comparison to control. The highest and lowest values were in the control treatment and 1000 mg/kg of the extract, respectively. There was no significant difference between white blood cells (p>0.05). The extract effect on red blood cells, lysozyme, IgM, hematocrit, MCV, MCHC, and hemoglobin in the 1000 mg/kg group showed significant differences with other groups (p<0.05). The percentage of neutrophils in all treatments was significantly higher than in the control group (p<0.05). According to the results, the addition of withania coagulans extract to the diet can improve blood indicators, immunity index, and growth parameters of common carp fingerlings.

Salinity is one of the stressful and effective factors in the life, metabolism and distribution of aquatic animals that affects the animal's growth process. Therefore, in this study, the effects of different salinity on growth factors, survival and blood indices of rainbow trout were studied.

In this study, 450 rainbow trout with average weight of 5.53 ± 0.057g were divided into four groups with salinity of 5, 13, 20, 30 ppt and fresh water group of each group with three replications during 2 months were studied.At the end of the research growth indices survival and blood indices were measured.

The results of this study showed that increased salinity of the water caused a decrease in body weight, SGR and CF, although weight loss in different groups was not significant (P> 0.05).In addition, salinity affected the survival of rainbow trout and reduced the survival of fish.Also, with increasing salinity, the levels of red blood cells, hemoglobin and hematocrit increased and salinity was 20 grams per liter, with the highest amount. The highest number of white blood cells was in the fish of the salinity group of 13 ppt and the lowest number of these cells in salinity was 20 ppt, which showed a significant difference (P <0.05).

Rainbow trout can grow up to 20 ppt in saline water, but with increasing salinity, the base metabolism of this fish increases, and the decrease in growth indices It is observed and its blood parameters change.

Environmental, nutritional, size and age conditions cause many direct and indirect changes in the blood and serum indices of fish in different species. The aim of this study was to determine and evaluate the changes in hematological indicators and some biochemical indicators of Cyprinus carpio blood serum in different weights.  For this purpose, common carp farmed in the weight range of 500 to 2000 g were examined and analyzed in 5 weight groups of 500, 700, 1000, 1300 and 1600 g.  The studied fish were bred and examined in a 2-hectare pool in one of the farms of Sari city under the same conditions. To perform blood tests, 5 fish were used in each weight group and blood sampling was performed by cutting the vein of the caudal stem vein. The results of the study of hematological indicators and some serum biochemical indicators showed that in different weights, significant changes in the amount of red blood cells, hemoglobin, hematocrit, mean red blood cell volume, mean hemoglobin concentration, mean red blood cell hemoglobin concentration, white blood cells, lymphocyte, monocyte, granulocytes, glucose, urea, triglycerides, cholesterol, phosphorus, calcium, sodium, potassium, albumin, and total protein were not observed (P> 0.05). The results of the present study showed that weight changes did not affect the hematological indicators and some biochemical indicators of common carp blood serum.

In this study, the effects of changes in environmental salinity on some biochemical parameters and body composition in yellowfin seabream (Acanthopagrus latus) were assayed. Fish adapted to salinity of 20 ppt, then suddenly introduced in experimental salinities including 5, 12 and 34 ppt. Each treatment was done in three replicates. In each replicate, 12 fishes with an average initial weight of 100± 10 grams per 300-liter tanks were placed. alues increased in group 12 and 34 ppt (P<0.05). A higher level of triglyceride and lower levels of high density lipoprotein were recorded in 34 ppt compared to other treatments (P<0.05). The proximate composition of body was affected by salinity as moisture was lower in 34 ppt in compare to other groups (P<0.05). In this study, A. latus survived without mortality in experimental salinities, but changes induced for osmoregulation in 5 ppt requires high energy consumption, which reduces the energy available to the fish.

Inroduction & Objective: Vitamins are organic compounds, which are vital for fish, and their shortages lead to severe disorders in the body of the fish, and since fish only absorb vitamins through food, this study examines the effects of different levels of vitamins C and E on blood and immunity factors of common carp (Cyprinus carpio). The present study was carried out with 9 diets containing 0, 200 and 400 mg / kg of vitamin C and 0, 200 and 400 mg / kg of vitamin E for 6 weeks. At the end of the blood sampling from fish blood stem vein removal was performed and blood, enzyme and immune factors were measured in a hematologic laboratory. Blood analysis showed that there was a statistically significant difference between the groups in terms of RBC, hemoglobin, hematocrit, lymphocyte, monocyte, eosinophil, neutrophil, MCH and MCV (P <0.05). There was no significant difference in MCHC between the groups (P> 0.05). By adding vitamins C and E, the levels of lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase decreased, and the activity of lysozyme, ACH50, and IGM increased. The results of this study showed that in most of the indices, 400 mg / kg of vitamin C and E supplementation improves performance. Adding different amounts of vitamins C and E will increase blood factors and improve immune function.

In this study, the effect of stocking density on some hematological (Red and white blood cells) and serum biochemical parameters (Glucose, triglycerides, cholesterol, total protein, inorganic phosphorus, calcium and magnesium, enzymes alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase and cortisol) of Carassius auratus was investigated. Fish with average weight 200±18 g were stocked randomly into 6 tank with triplicate in two treatments for a 30-day period and were submitted to different stocking densities: low density (T1, 10 kg/m3) and high density (T2, 45 kg/m3). At the end of the trial, Fish from each group were selected and their blood was collected and the following parameters were analyzed. Fish held at high density showed significant higher levels of cortisol, glucose and alkaline phosphatase (23±0.4 ng/dl, 52±3.2 mg/dl and 93.2±8.1U/L, respectively) compared with those held at low density (36±0.9 ng/dl, 83±5.2 mg/dl and 61.2±6.3U/L, respectively), while the levels of triglycerides significantly decreased with increasing density (P

This investigation was carried out to assess the over-ripening phenomenon and its relationships with sex steroids levels including testosterone (T), progesterone (P), 17-beta estradiol (E2) and some important liver enzymes such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as to determine the levels of stress indices and blood biochemical parameters including cortisol, glucose, lactate total fat, total protein and calcium in cultured Sterlet sturgeon (Acipenser ruthenus) broodstocks. For this study, 18 fish with an average weight of 1120.2 ± 240.4 g were selected. Blood samples were taken from three groups (each group with six fish) in three stages including non-overripe, beginning the overriping, and completely–overripe process. Comparing the three groups showed significant differences in plasma levels of T, E2, AST, cortisol, glucose and lactate, with the highest levels of AST, cortisol, and lactate with values in completely-overripe stage, and the lowest levels of in non-overripe stage. The highest levels of glucose, T, and E2 were found in non-overripe stage (P0.05). This study showed that steroid hormones and biochemical indices could be more reliable indicators for forecasting and assessment of ripening stage of sturgeon and these factors can be used as biomarkers to control reproduction and ripening times in cultured Sterlet sturgeon broodstocks.

In current study, after one week of adaptation, 250 common carp (Cyprinus carpio) were fed with two levels of Lactobacillus probiotic (106, 107) for 42 days and exposed to 50% lethal concentration of silver nanoparticles at for 10 days. Treatments included a control group, a probiotic group of 106, a probiotic group of 107, a group exposed to silver nanoparticles, a group of exposed to silver nanoparticles and fed with probiotic 106, and a group exposed to silver nanoparticles and fed with probiotic 107. Results showed that there was significant differences in MCV, MCHC, white blood cell, monocyte, neutrophil, hemoglobin, red blood cells and hematocrit between the control group and the treatments (P <0.05). Probiotics had a depletion effect on hemoglobin, red blood cells, hematocrit, and an increased effect on MCV, MCHC, white blood cells, monocytes and neutrophils. Additionally, silver nanoparticles showed depletion effects on hemoglobin, red blood cells, hematocrit, white blood cell monocyte and hemoglobin (MCHC) and increased effect on MCV and neutrophil volume. Altogether, results showed that probiotic the destructive effects of nanosilver, which led to the reduction of hemoglobin in blood cells (hypocoemic normocytic). Moreover, higher levels of probiotic (treatment 6) resulted in an increase in hemoglobin of blood cells (hyperchromic urodynamics) which requires further studies in this field.

Inroduction & One way to evaluate the physiological characteristics of the fish to determine the blood that is superior to other methods, easier and less costly. In this study, determination and comparison of some blood factors rainbow trout fed with manual and commercial (pellet) food diet was done.
Testing for 8 weeks in both groups, with a total of 60 fish from each group were done. Blood samples were collected from the fish using the caudal vein cut and each blood parameters in hematology laboratory values were measured.
: the results of this study showed that blood on the white blood cells, red blood cells, hemoglobin, hematocrit trout fed commercial diets and manual significant difference(P 0.05) and the mean corpuscular volume, corpuscular hemoglobin, neutrophils, lymphocytes and eosinophils, there is no difference(P> 0.05). The results showed that blood biochemical parameters of glucose, cholesterol, triglycerides, creatinine, phosphorus, chloride, iron and albumin significantly different(P 0.05), but the amount of calcium, potassium, sodium, urea and total protein no there is no significant difference(P> 0.05). Also, in terms of Spar aminotransferase and alanine aminotransferase significant difference between the two groups(P 0.05).
The results of this study suggest that the type of diet can affect blood indices fish.

Inroduction & Mineral materials are the most important part of the fish nutrition subjects diet because they represent a very low quantity in the diet but they have a high effect on fish physiology and metabolism. The aim of this study was investigate the effect of different level of zinc sulfate (ZnSo4) on hematocrit and hematology for assess the effects of this mineral matter on goldfish health.
Experimental diets prepared with four levels(four treatments and each treatment include three repetitions) 0, 25, 75 and 125 mg/kg. Healthy fish with average weight 3.3 ± 0.10 and average length 3.5 ± 0.2 was maintained in 400 liter tanks with temperature 28 ± 1, pH 7.5 ± 0.5 and hardness 286 ± 1.2 and feed for 60 days and end the trial hematocrit and hematology parameter was measured.
Result of analysis the hematological parameter of fish feed with different levels of zinc showed that white blood cells (WBC), mean corpuscular volume (MCV), mean cell hemoglobin (MCH) and mean cell hemoglobin concentration (MCHC) in our experimental treatments did not show a significant difference (p≥0.05) but in red blood cells (RBC), hematocrit (Ht) and hemoglobin (Hb) there was significant difference between treatments (p≤0.05). Red blood cell count, hematocrit and hemoglobin in zinc sulfate treatments (treatments 2, 3 and 4) had higher values and the highest value was in group 4 (150 mg kg zinc sulfate), which there was significant difference between 2 and 3 treatments also had a significant difference with control group.
The results shows that zinc in the diet stimulate the immune system and can have positive effects on the health and hematological factors of goldfish.

The present study was performed to investigate the effects of various levels of dietary lactobacillus acidophilus as feed supplement on the growth performance, survival rate, hematological parameters and intestinal microbiota of guppy (Poecilia reticulata). For this purpose onethousand and eight hundred guppy larvae with 7 mg mean weight in a completely randomized design with 4 treatments and 3 replicates of L. acidophilus in the diet, including no bacteria (control), 1.5×107, 3×107, 6×107 CFU g-1(T2,T3,T4 respectively) were considered and fed for 16 weeks. The results showed that significant differences (P0.05) with control (5.96±0.2). The highest amount of intestinal of Lactobacillus was observed in 4 group compared to other groups and control (P

Considering the effects of different temperature regimes on blood parameters of sturgeons during transport, this study was conducted to determine the effect of different temperature regimes (12, 20, 23 and 28 °C) on blood parameters of Sterlet at 0 (control), 1, 5 ,24 and 48 hour After transportation. After transport blood parameters including red blood cells, white blood cells, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, lymphocytes and neutrophils in treatments with high temperatures (23 and 28 °C) showed a significant difference from the control (P0.05). The results showed that temperature range of 20-23 °C can be used for reduce stress and physiological consequences on Sterlet after short-term transportation.

Malathion is a pesticide that is commonly used in agriculture in Iran and its entrance to the aquatic ecosystems resulting in numerous physiological effects on the fishes. In order to investigate the effect of malathion poison on hematological parameters of rainbow trout, 3 sub lethal concentrations (0.025, 0.05, 0.075 mg/l) and a control (each with three replications) were selected and blood tests were performed on the fifth and ninth days. The exposed fish to sub-lethal concentrations of pesticide malathion resulted in a significant reduction (P

In the present study, we investigated the effects of a sub-acute concentration of cypermethrin on some hematological parameters of Capoeta damascina. The specimens had an average weight of 150 ± 10 g and average length of 21 ± 1 cm (mean ± SD). The fish were exposed to 0, 0.25, 0.5, and 0.75 µg/L (each with 3 replicates) for 10 days. Blood samples were collected on days 1, 5 and 9. Hemoglobin (Hb) and hematocrit (Hct), red blood cell count (RBC), white blood cell count (WBC), mean cell volume of red blood cells (MCV), and mean corpuscular hemoglobin concentration (MCHC) were measured. There was a significant decrease in RBC, Hct, MCV and Hb of the specimens exposed to cypermethrin compared with those of the control group. Therefore, this study has indicated that environmental pollutants cause negative effects on hematological parameters of Capoeta damascina and impairment of these parameters may initiate other biological complications.

In the present study, we evaluated the pathogenicity of Yersinia ruckeri on Persian sturgeon (Acipenser persicus) fingerlings. We divided the sturgeon into one treatment group, one positive control group and one negative control group (with three replicates for each group). The treatment group juvenile Persian sturgeon received 0.1 ml of bacterial suspension (2.8 × 106 cells/fish) via an intraperitoneal injection. Positive control fish received 0.1 ml intraperitoneal injection of sterile normal saline. The negative control group received no injection. According to the results, first mortality was observed 48 h post-challenge in the treatment group. Mortality rates in the treatment group post-challenge were 6.7% (48 h), 26.6% (72 h), 60% (96 h), and 70% (7 days). Haemorrhage in terminal part of intestine and accumulation of bloody fluid in the intestinal lumen were observed in the majority of sick fish. Intestinal histopathology was characterized by spread necrosis in the epithelium of the villi and lymphocytic infiltration to the connective tissues. In the haematological study, we observed significant differences in RBC, WBC, MCH, MCHC, hemoglobin, and hematocrit counts between fish from the treatment and control groups.

Evaluation of probiotic is not summarized on growth but included the other aspects, such as blood parameters. In this study, effects of two bifidobacter, Bifidobacterium animalis and Bifidobacterium lactis with three concentrations of 1× 109 (T1), 2× 109 (T2) and 3× 109 (T2) CFU/100g were studied on growth performance and hematological parameters of rainbow trout larvae with initial body weight of 0.538 ± 0.197 g. Thus 3 experimental treatments and a control, each with three replicates were prepared. Thirty rainbow trout larvae were stocked in each replicate. The larvae were fed 4 times a day according to 4-8% of body weight. After 8 weeks of feeding trail with supplemented diet with Bifidobacterium growth and hematological parameters were studied. The result of hemoglobin showed no significant difference between experimental treatments and the control (P>0.05). The use of Bifidobacter caused increase of red and white blood cells in T3 and T2 treatments (P<0.05). However no significant difference was observed in MCV and MCH (P>0.05). The study of hematological parameters, final weight and specific growth rate in present study showed that the use of bifidobacter increased blood cell account and also the final weight growth in rainbow trout.

Sturgeons are of commercially important fish species in the Caspian Sea, but there are few reports about their oxygen requirements. In the present study, the effect of different oxygen levels on hematological parameters were examined in two weight classes of great sturgeon Huso huso including small size (average initial weight 280.9 ± 49.2 g) and large size (average initial weight 1217.9 ± 138.1 g). Oxygen treatments including Hypoxia (2-3 mg/l), Normoxia (5-6 mg/l) and Hyperoxia (9-10 mg/l) were set up with managing inflowing water and using system equipped with oxygen enriching for Hyperoxygenation (pure oxygen injected). Fish were acclimated to experimental tanks for one week and then randomly were transferred to 9 tanks for each class (3 fish per tank in higher weight class and 6 fish per tank for lower one) and were kept for 8 weeks. HCT and MCHC at lower weight class and RBC, MCV and Neutrophil at higher weight class were significantly different between treatments.