جستجوی مقالات مرتبط با کلیدواژه « استافیلوکوکوس اورئوس » در نشریات گروه « شیمی »

The increasing development of antibiotic resistance of bacteria has provided the basis for replacing antimicrobial agents with plant origin and with less side effects. This research is a type of laboratory study that was conducted with the aim of determining the antibacterial effect of Myristica fragrans on staphylococcus isolates beta lactamase producing antibiotics. The methanol extract of the plant was prepared by maceration method. The extract was filtered with Whatman No.1 paper and concentrated by rotary evaporator system. Different concentrations of 200, 100, 80, 40, 20, 10, 5, 2.5, 1.25, 0.625 of the extract were prepared in DMSO: Methanol (1:1v/v) solvent. Identification of beta-lactamase producing isolates was done by phenotypic method with cefotaxime antibiotic discs and cefotaxime / clavulanic acid combined disc. Antibacterial activity against 40 isolates of beta-lactamase-producing isolates was investigated by agar wells diffusion method. After incubation for 24 hours at 37°C, the sensitivity of bacteria was determined by measuring the diameter of the growth inhibition zone. Based on the results, out of 60 Staphylococcus aureus bacteria, 67% of isolates were beta-lactamase producers, respectively. All isolates of Staphylococcus aureus showed sensitivity to Myristica fragrans extract, and the average of minimum growth inhibition concentration to beta-lactamase-producing Staphylococcus aureus was 10 mg/ml. Due to increasing antibiotic resistance, it seems that Myristica fragrans extract can be used against beta-lactam-resistant Staphylococcus aureus strains in controlling infections, and in this regard, isolation and identification of the effective substances of the plant extract it is suggested.

pathogenic bacteria in food are the cause of many infections and food poisoning. The prevalence of bacterial contamination is high in developing countries, and Staphylococcus aureus, Salmonella and Escherichia coli account for the highest amount of food contamination; Therefore, the aim of this study is to investigate the prevalence of Escherichia coli, Staphylococcus aureus and Salmonella in water, fruit juice and traditional ice creams offered in Qom city, Iran.

In the present study, 300 samples, including 100 water samples, 100 fruit juice samples, and 100 traditional ice cream samples, were taken from supply centers, sampled and transferred to the food hygiene laboratory of Shahrekord Azad University. SPSS version 26 statistical software and Kai-Hat statistical test were used for data analysis. A significant level (P<00.05) was considered.

The results showed that out of 300 studied samples, 170 samples were infected with pathogenic microorganisms. In this way, the amount of Escherichia coli, Staphylococcus aureus and Salmonella contamination in water was 1, 16 and 2 samples, in traditional fruit juice 8, 46 and 26 samples and in traditional ice cream 8, 59 and 28 samples.

According to the present results, it is necessary to refrain from consuming traditional fruit juice and traditional ice cream in centers that have unfavorable health conditions and to use purified water, and if there is no purified water before Boiled water should be consumed to prevent food-borne diseases.

Cheese is a product consisting of fat and milk protein, along with calcium and phosphorus that are combined with milk protein in various ways. Due to the importance of cheese consumption among the people of Iran and considering that this product is one of the most important dairy products and according to available statistics, about 20% of the milk produced in the dairy industry is converted into cheese, of which the share of production Traditional cheese is about 80%. The purpose of this study was to evaluate the level of contamination of traditional cheeses offered in different areas of Karaj. The present descriptive cross-sectional study was conducted in the summer of 1398 by sampling different parts of the north, west, east, south and center of Karaj . Four types of bulk cheeses of Tabriz Ligvan, lactic, white and gypsum in five geographical areas with three replications were examined for the presence of Staphylococcus aureus. The results shows the contamination of traditional cheeses and its different areas, although the rate of contamination in the west was higher than other areas. Among the regions, the eastern region with the lowest level of pollution among other regions and the presence of contamination only in gypsum cheese, was identified as the least dangerous region. In contrast to the existence of high pollution in all four types of cheese studied in the west. based on the results, this part of Karaj was introduced as the most dangerous area in the consumption of traditional cheeses.

Due to the increasing resistance of pathogenic bacteria to common antibiotics, researchers are looking for antimicrobial agents of plant origin as alternative drugs. The aim of this study is to investigate the antimicrobial activity of peperin isolated from black pepper on Staphylococcus aureus isolated from the nose and throat. Sampling was done from the pharynx and nose of the patients and 9 strains of Staphylococcus aureus were isolated and purified. The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MBC) in six concentrations were evaluated by dilution method in liquid medium on the determined bacteria. The results of this study showed that the lowest inhibitory concentration was equal to 8 μg/ml, which inhibited one strain, while the highest concentration was 512 μg/mg, which inhibited one strain. Staphylococcus aureus isolated the most It was resistant to trimethoprim (88.8%), ampicillin (77.7%), tetracycline (77.7%), erythromycin (66.6%) and ceftazidime (22.2%). The results of this study showed that piperine present in black pepper has a very strong inhibitory effect on Staphylococcus aureus bacteria isolated from the nose and throat.

Contamination and spoilage of food are important in many ways, such as health and the economy. Identification of food contamination can significantly help to improve food production and distribution processes and prevent the wastage of many resources. In Iran, many researches have been carried out in this field during continuous periods of time, and a careful examination of these researches can provide valuable information. In this article, an attempt has been made to briefly review the types of food contamination in the dairy, meat, and confectionery categories in recent years in Iran and provide a bibliometric report of the documents published in the Scopus database without a time limit. The results of the investigations revealed that Escherichia coli and Staphylococcus aureus bacteria are probably more important than other bacteria in this country. The low ratio of the number of review documents to articles and also the lack of publication of no book in this database can be of interest to researchers to collect, summarize and conclude this information, which will definitely be useful.

This descriptive cross-sectional study was performed to investigate the presence or absence of bacterial contamination caused by Escherichia coli and Staphylococcus aureus in creamy sweets in the west of Tehran. From 12 selected confectioneries in the west of Tehran, about 500 grams of creamy sweets equivalent to 3 pieces (solid food sample) (according to the instructions of the standard number of samples required in food sampling) were prepared using simple random sampling method. In this study, in order to isolate and identify Escherichia coli bacteria, lauryl sulfate tryptase culture medium was used. Due to gas production and turbidity observed in all samples (36 samples), EC Broth selective culture medium was used for complementary and confirmatory steps. peptone water culture medium was used for confirmatory tests of Escherichia coli and differentiation from other fecal coliforms, coaxial reagent was used for final confirmation. Isolation of Staphylococcus aureus bacteria was done using Cooked Meat enrichment medium and due to the production of spherical or oval or spindle colonies that were created in the depth of the medium in all samples, from Baird Parker Agar culture as The base was used for the second stage and in continuation of coagulase test to confirm Staphylococcus aureus bacteria from other staphylococci, rabbit citrate plasma was used for confirmatory tests. By reviewing the data, the rate of bacterial contamination caused by Staphylococcus aureus in 36 samples was equal to 25% and the rate of bacterial contamination due to Escherichia coli in 36 samples was equal to 5%.