جستجوی مقالات مرتبط با کلیدواژه « bioremediation » در نشریات گروه « محیط زیست »
تکرار جستجوی کلیدواژه «bioremediation» در نشریات گروه «علوم پایه»-
سابقه و هدف
آلودگی خاک به فلزات سنگین بویژه در مناطق صنعتی یکی از اصلی ترین مشکلات محیط زیستی به شمار می آید. کادمیوم یک فلز سنگین است که در گیاهان تنش اکسیداتیو ایجاد می کند و اثرات مخرب بسیاری بر کیفیت محصول دارد. امروزه روش های مختلفی جهت کاهش اثرات منفی غلظت های بالای فلزات سنگین موجود در خاک مورد استفاده قرار گرفته است. در این راستا، استفاده از بیوچار، روشی مقرون به صرفه و سازگار با محیط زیست است که تاثیر آن به منظور کاهش قابلیت دسترسی زیستی فلزات سنگین در خاک یک مزیت مهم به شمار می رود.
مواد و روش هادر این مطالعه، آزمایشی گلخانه ای به صورت فاکتوریل در قالب طرح بلوک کامل تصادفی با سه تکرار و با نمک کلرید کادمیوم در شش سطح (0، 1، 3، 5، 7 و 10 میلی گرم بر لیتر) و سه سطح بیوچار (0، 5/1 و 3 درصد وزنی) بر گیاه دارویی همیشه بهار (Calendula officinalis L.) انجام شد. تاثیر تیمارهای آزمایش به طور جداگانه و ترکیبی بر خصوصیات مورفولوژیک (وزن تر و وزن خشک اندام های هوایی و ریشه)، فیزیولوژیک (مقادیر کلروفیل a، کلروفیل b، کلروفیل کل و کاروتنوئید) و بیوشیمیایی (قند محلول، کاتالاز، پراکسیداز و پرولین) این گیاه دارویی مورد بررسی قرار گرفت. مقایسه میانگین ها، با آزمون چند دامنه ای دانکن در سطح احتمال پنج درصد صورت پذیرفت.
نتایج و بحث:
نتایج نشان داد که غلظت های مختلف کادمیوم سبب کاهش وزن تر و خشک ریشه و اندام های هوایی گردید. بیشترین تاثیر کاهشی، مربوط به غلظت 10 میلی گرم بر لیتر کادمیوم بود. اثر متقابل بیوچار و کادمیوم فقط بر وزن خشک اندام های هوایی گیاه معنی دار شد. تاثیر تنش ناشی از افزایش غلظت کادمیوم بر فرآیندهای فیزیولوژیک گیاه متفاوت بود. به این ترتیب که با افزایش غلظت کادمیوم، روند کاهشی در محتوای کلروفیل و روند افزایشی در مقادیر کاروتنوئید و صفات بیوشیمیایی گیاه مشاهده شد. با توجه به اینکه اولین اثر کادمیوم بر گیاه، کاهش فتوسنتز و کلروز برگ ها می باشد، در بالاترین سطح کادمیوم، میزان کلروفیل کل نسبت به شاهد 40 درصد کاهش، اما میزان کاروتنوئید 50 درصد افزایش یافت (05/0>p). کاهش محتوای کلروفیل تحت تنش کادمیوم می تواند به دلیل آسیب های اکسیداتیو و بازدارندگی مراحل مختلف سنتز کلروفیل باشد. اما افزایش کاروتنوئیدها در مواجهه با تنش فلزات سنگین به این دلیل است که کاروتنوئیدها به عنوان مولکول های دخیل در سیستم دفاع آنتی اکسیدانی غیر آنزیمی، نقش حفاظتی در برابر تنش اکسیداتیو دارند. در مقابل، استفاده از تیمار بیوچار سبب افزایش معنی دار وزن تر اندام های هوایی، میزان کلروفیل a و کاروتنوئید گردید. همچنین، اثر متقابل تیمارها حاکی از این بود که در سطوح مختلف کادمیوم، با افزایش سطح بیوچار، وزن خشک اندام های هوایی، میزان کلروفیل b و کلروفیل کل روند افزایشی داشتند. از صفات بیوشیمیایی مورد بررسی، اثر متقابل تیمارها فقط بر میزان قند محلول (05/0>p) و کاتالاز (01/0>p) معنی دار شد. این یعنی جذب کادمیوم توسط بیوچار و کاهش اثر سمیت آن روی گیاهچه ها، شرایط تولید بیشتر قند محلول و کاتالاز را فراهم نمود. در واقع، بیوچار به دلیل ظرفیت تبادل کاتیونی بالا، سطح ویژه بالا و وجود گروه های عاملی، جذب مناسبی از کادمیوم داشت.
نتیجه گیریدر مجموع، نتایج به دست آمده بیانگر توان بیوچار در تثبیت و غیرقابل جذب نمودن کادمیوم بود. از این رو، استفاده از بیوچار در خاک می تواند دسترسی زیستی کادمیوم توسط گیاه در فرآیند گیاه پالایی را بهبود بخشد، هر چند که تاثیر نوع و واریته گیاه بر میزان کاهش تنش کادمیوم نبایستی نادیده گرفته شود.
کلید واژگان: کادمیوم, فلز سنگین, بیوچار, زیست پالایی, گیاه همیشه بهارIntroductionSoil pollution by heavy metals, especially in industrial regions is one of the main environmental problems. Cadmium (Cd) is a heavy metal that causes oxidative stress in plants and has many destructive effects on product quality. Nowadays, various methods are used to reduce the negative effects of high concentrations of heavy metals in the soil. In this regard, using biochar is a cost-effective and environmentally-friendly method and its influence on the reduction of heavy metals bioavailability of soil is an important advantage. Biochar is a carbon-rich material obtained by pyrolysis of biomass, such as agricultural residues and manures in conditions without oxygen or with limited oxygen content.
Material and MethodsIn this study, a factorial experiment was conducted in a completely randomized blocks design with three replications on Marigold (Calendula officinalis L.) medicinal plant with six levels of Cd (0, 1, 3, 5, 7, and 10 mg/l) and three levels of biochar (0, 1.5, and 3 w/w). The effect of experimental treatments was investigated separately and combined on the morphological (wet and dry weights of aerial parts and roots), physiological (the amount of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid), and biochemical (soluble sugar, catalase, peroxidase, and proline) characteristics of this medicinal plant. Means comparisons were done by Duncan's multiple range test at a probability level of 5%.
Results and DiscussionThe results showed that different concentrations of Cd decreased the wet and dry weights of roots and aerial parts of the plant. The most reduction effect was related to the concentration of 10 mg/l of Cd. The interaction effect of biochar and Cd was significant only on the dry weight of aerial parts. The effect of stress caused by increasing the concentration of Cd on the plant's physiological processes was different. Considering that the first effect of Cd on the plant is the reduction of photosynthesis and chlorosis of the leaves, at the highest level of Cd, the amount of total chlorophyll decreased by 40% compared to the control sample, but the amount of carotenoid increased by 50% (p<0.05). The reduction of chlorophyll content under Cd stress can be due to oxidative damage and inhibition of different stages of chlorophyll synthesis. However, the increase in carotenoids in response to heavy metal stress occurs because these molecules, as part of the non-enzymatic antioxidant defense system, play a protective role against oxidative stress. In contrast, the use of biochar treatment caused a significant increase in the wet weight of aerial parts, the amount of chlorophyll a and carotenoid. Also, the interaction effect of the treatments indicated that at different levels of Cd, the characteristics of the dry weight of aerial parts, the amount of chlorophyll b, and the total chlorophyll increased with the increase in the biochar level. Among the investigated biochemical traits, the interaction effect of the treatments was significant only on the amount of soluble sugar (p<0.05) and catalase (p<0.01). This means that the absorption of Cd by biochar and the reduction of its toxicity effect on seedlings provided the conditions for more production of soluble sugar and catalase. In fact, biochar had high adsorption of Cd due to its high cation exchange capacity, high specific surface, and presence of functional groups.
ConclusionIn total, the results showed the biochar capacity to stabilize and inactivity Cd absorption. Therefore, the incorporation of biochar to soil can improve Cd bioavailability by plants in the phytoremediation, although the effect of type and plant variety on the amount of decreasing Cd stress should not be ignored.
Keywords: Biochar, Bioremediation, Cadmium, Calendula Officinalis, Heavy Metal -
Global Journal of Environmental Science and Management, Volume:10 Issue: 4, Autumn 2024, PP 1561 -1572BACKGROUND AND OBJECTIVESThe presence of heavy metals, specifically cadmium, in the environment poses significant threats to both ecological systems and human health. However, microalgae have shown potential in addressing this issue through their ability to absorb cadmium and produce valuable biomass, making them a promising solution for bioremediation. Among the various microalgae species, Chlorella vulgaris stands out as a suitable candidate due to its potential for biodiesel production and its capacity to effectively absorb cadmium. Therefore, the main objective of this study is to assess the toxicity of cadmium on Chlorella vulgaris cells using network meta-analysis as a methodology.METHODSA comprehensive search was conducted on Scopus, Scilit, Google Scholar, and Web of Science to identify relevant studies published from 1 January 1990 to 16 January 2024. Only studies that reported the cell number of Chlorella vulgaris as a result of cadmium exposure were considered for inclusion. The collected data were then subjected to Bayesian frequentist network meta-analysis, utilizing standardized mean difference and a 95 percent confidence interval as measures of effect size. Additionally, a linear regression analysis was performed to examine the dose-dependent impact of cadmium toxicity.FINDINGSDose-dependent toxic effects of cadmium on Chlorella vulgaris were evident (R-square of more than 0.90), particularly at a concentration of 1 part per million, deemed as the maximum tolerable threshold. Prolonged exposure revealed a concentration-dependent reduction in cell viability, suggesting potential lifespan shortening. A comparison of growth stimulants, gibberellic acid and brassinolide (standard means differences of 1.7 and 3.8, respectively), in mitigating cadmium toxicity indicated the latter superior effectiveness in sustaining microalgal survivability. The presence of high nitrogen and low phosphorous levels was found to be significantly associated with a reduction in Chlorella vulgaris cells due to cadmium exposure.CONCLUSIONThis research has provided conclusive proof of the harmful effects of cadmium on Chlorella vulgaris through the implementation of Bayesian frequentist network meta-analysis, offering valuable insights for environmental management practices. The findings reveal concentration-dependent effects of cadmium toxicity. The survivability of Chlorella vulgaris is determined by the compositions of macronutrients nitrogen and phosphorous. Comparative analyses highlight the superior protective effect of brassinolide over gibberellic acid in mitigating cadmium toxicity. Overall, the findings highlight the potential of Chlorella vulgaris in both bioremediation of heavy metals and biomass production.Keywords: Bioremediation, Cadmium, Chlorella Vulgaris, Growth, Heavy Metals
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Global Journal of Environmental Science and Management, Volume:10 Issue: 3, Summer 2024, PP 1151 -1170BACKGROUND AND OBJECTIVESThe quality and production volume of the cultivation of tiger prawn Penaeus monodon have decreased considerably in the last two decades. However, intensification and extensification efforts, including the application of cultivation technology through pond land recovery, have not produced expected results. Visible symptoms suggest potential issues with the cultivation water possibly originating from exposure to heavy metal pollutants. Therefore, this study aimed to remove heavy metal pollutants by using sponge symbiont bacteria bioremediators to increase the survival rate and quality of tiger prawn production. The achievements of this research are expected to contribute to the scientific development of environmental microbiology, bioremediation, and aquaculture pollution control.METHODSThe study utilized Bacillus pumilus and Pseudomonas stutzeri bacteria. The water used for tiger prawn post-larvae cultivation was treated with these bioremediator bacteria. The water had copper and lead ion concentrations that were 20 times greater than the maximum threshold value. The physical and chemical characteristics and parameters, such as dissolved organic matter, nitrite, nitrate, and ammonia contents, of the cultivation water were monitored over a 30-day period. The specific growth rate in terms of weight and body length and the survival rate of the tiger shrimps were measured to evaluate the effect of the bioremediation process on the prawns. The concentrations of copper and lead ions in the cultivation water and within the body of the tiger shrimps were analyzed. The health of the tiger prawns was evaluated by observing signs of tissue damage.FINDINGSAmong all the treatments, Treatment I with copper ion exposure had the highest average specific growth rate of the tiger prawns in terms of weight and body length, followed by Treatment II with lead ion exposure and Treatment III with a combination of both pollutants (the lowest). The intersection of copper and lead ion concentrations in the tiger prawns and cultivation media occurred in the cultivation period of 15–20 days. The use of Bacillus pumilus and Pseudomonas stutzeri bacteria as bioremediators effectively remediated the copper and lead pollutants at an average of 99.34 percent and 97.18 percent of the initial concentration, respectively. Despite the bioremediation efforts, the tiger shrimps exhibited symptoms of tissue damage in the head, tail, and shell. These symptoms included necrosis, myopathy, and infiltration, which are indicative of decreased cell function due to the presence of toxic agents.CONCLUSIONBioremediation with Bacillus pumilus and Pseudomonas stutzeri bacteria helped reduce the accumulation of heavy metal pollutants. However, negative effects on the health and growth of tiger prawns were still observed when the prawns were exposed to copper and lead ion concentrations below the allowed threshold value.Keywords: Bioremediation, Cultivation media, Heavy metal pollutants, Sponge symbiont bacteria, Tiger prawns
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Pollution, Volume:10 Issue: 3, Summer 2024, PP 1007 -1018The current study tested the ability of the aquatic plant Azolla pinnata to remove the pyrene and determine the effect of Pyrene on the pigments (chlorophyll a, b, and carotenoids) and antioxidant parameters (CAT, SOD, MDA and, ROS) in the plant. The plant was exposed to different concentrations of Pyrene (1,5,10,15,25, and 50 mg/l) for 14 days. The results showed that the high removal rate of pyrene reached 99% for 25 and 50 mg/l of pyrene on the 14th day of the experiment, while the lowest removal rate was 42.1% for 1 mg/l on the third day.The results showed the lowest values of total chlorophyll, chlorophyll a and carotenoids were 0.55, 0.25, and 0.433 μg/g recorded at 50 ppm respectively. While chlorophyll b recorded a low value of 0.32 μg/g at 25 mg/l, the highest concentration of these pigments was registered in the control group. Also, the results showed a gradual increase in antioxidant values compared to the control group, recording the highest values of 0.2873 and 1.04 U/g at 50 ppm and the lowest value of CAT and SOD at 5ppm, compared to the control group. Also, the study recorded a gradual increase in ROS and MDA values with increasing concentrations of pyrene, the highest value was 1.6367 and 14.4433 µmole/g for ROS and MDA at (50ppm), whereas the lowest value was recorded at 5 ppm compared to the control group. Statistical analysis at (p˂0.05) showed significant differences between all the interactions.Keywords: Azolla Pinnata, Bioremediation, Pahs, Pyrene
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Environmental pollution has posed a major threat to terrestrial, aquatic, and marine ecosystems, thereby affecting microflora and micro-fauna populations. This study assessed the growth attributes of maize plants on crude oil-polluted soils amended with agro-wastes. Six kilograms each of composite soil sample was weighed and transferred into one hundred and fifty labeled plastic buckets with drainage holes for soil aeration and spiked with 300mls each of crude oil, allowing for 14 days of soil acclimatization. Soil amendments such as groundnut husks, cassava peels, empty fruit bunch of oil palm, and maize cob powder were applied and allowed for 90 days. Maize seeds were sowed, while periodic data were collected and subjected to a three-way ANOVA. The result obtained revealed that maize seeds grown on agro-wastes treated and pristine control soils show early seed germination than the crude oil-polluted control soil. The plant height obtained for GnH14P + MaC14P at 10% was the highest with a mean (of 152.81cm2), and the leaf area of the maize from soil treated with GnH14P + EFBOP14P at 10% had the highest mean (756cm2), the leaf length of maize from soil treated with GnH14P + CasP14P at 3%, 6%, and 10% was the highest with mean ranging (54-97 cm2) with no significant difference in mean values obtained. The stem girth, number of leaves, and leaf width were generally improved in the bio-remediated soils. The result for the yield performance of maize shows that the days to flowering were shortened in the bio-remediated soil compared to the prolonged flowering days observed in the crude-oil polluted control. The number of seeds per cob was high in the bio-remediated soils while no seed was obtained in the crude-oil-polluted control soils. It can be concluded that the ameliorated treatment with the agro-wastes improves the performance of maize plants in crude oil-polluted soils.Keywords: soil, hydrocarbons, maize, Agro-wastes, bioremediation
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Pollution, Volume:9 Issue: 4, Autumn 2023, PP 1741 -1753Bioremediation has become a trending and developing field in environmental restoration through the use of micro-organisms to utilize and reduced the concentration and toxicity of various chemical pollutants. This study is on bioremediation of hydrocarbon-polluted soils using some agricultural wastes. Ninety (90) plastic buckets were filled with 4kg each of the composite soil. The soil contained in the plastic buckets was spiked with 250ml crude oil, except in the unpolluted plastic buckets (0%) crude oil. The agro-wastes (plantain stem sap, bush mango peels, and fruited pumpkin husk powder) in single and combined forms were applied after 14 days soil pollution. The amendments were applied as follows: Pristine control (0% agro-wastes), crude-oil control (0% agro-wastes), 150g, 250g, and 350g of the agro-wastes. Soil samples were collected at 90 days for soil microbial counts and the total hydrocarbon content of the soil. Data collected were subjected to 2-way ANOVA. The result showed that the microbial population in the crude-oil polluted soil amended with different agricultural wastes significantly increased (p<0.05) the total heterotrophic and crude oil utilizing bacterial and fungal counts in the soils and the increase in microbial population result in a significant reduction in total hydrocarbon content (THC) of the soils. The reduction in the THC of the soil was treatment dependent. It is, therefore concluded that based on the efficiency of these agro-wastes in enhancing microbial degradation, further studies should be carried out on the enzyme activities and production of bio-surfactant from the wastes to shorten the degradation time.Keywords: Contamination, Amendment, bioremediation, fungi, Bacteria
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زمینه و هدف
یک روش جایگزین و سازگار با محیط زیست جهت پالایش محیط زیست از آلودگی ها، استفاده از مواد زیست فعال سطحی مشتق شده از میکروارگانیسم ها است. مزیت زیست فعال سطحی، زیست تخریب پذیری، سمیت پایین و اثر بخشی در افزایش تجزیه بیولوژیکی است. برخلاف سورفکتنت های شیمیایی، مواد فعال سطحی تولید شده از میکروب ها به آسانی تجزیه می شوند و به همین دلیل برای کاربردهای زیست محیطی به خصوص زیست پالایی بسیار مناسب اند. این پژوهش با هدف زیست پالایی و بررسی سینتیک جذب فلزات سنگین(کبالت و کادمیم) و رنگ رودامین B از آب توسط بیوسورفکتنت تولید شده از باکتری سودوموناس آیروژینوزا صورت گرفت.
روش بررسیدر این مطالعه از یک باکتری مولد بیوسورفکتنت که از سایت زباله کهریزک در جنوب تهران به عنوان سویه سودوموناس آیرژینوزا HAK02 جداسازی و شناسایی شده جهت تولید زیست فعال سطحی برای حذف آلاینده ها استفاده شد. تست زتا پتانسیل برای تشخیص بار بیوسورفکتنت تولید شده و استفاده در زیست پالایی انجام شد. میزان حذف رنگ توسط دستگاه UV و حذف فلزات سنگین به وسیله آنالیز ICP_AES انجام گرفت.
یافته هابا توجه به بار منفی ماده برای حذف فلزات سنگین و رنگ کاتیونی رودامین B استفاده شد. بیوسورفکتنت تولید شده با سودوموناس آیرژینوزا قادر به حذف 95% رودامینB ، 43%Cd2+ و 35% Co2+ شد. بررسی مدل سینتیکی جذب نشان داد با مدل سینتیکی شبه درجه دوم تطبیق بهتری داشت.
بحث و نتیجه گیریزیست پالایی با استفاده از زیست فعال سطحی جهت حذف فلزات سنگین و رنگبری روشی سریع و سازگار با محیط زیست می باشد. این نمونه جهت حذف رودامینB بسیار مناسب است و برای فلزات سنگین Cd2+ و Co2+ توانایی متوسط دارد.
کلید واژگان: رودامین B, زیست پالایی, زیست فعال سطحی, کادمیوم, کبالت و مدل سینتیکی جذبBackground and ObjectiveAn alternative and environmentally friendly method for purifying the environment from pollution is the use of biosurfactant derived from microorganisms. The advantage of biosurfactant is biodegradability, low toxicity, and effectiveness in increasing biological decomposition. Unlike chemical surfactants, surface active substances produced by microbes are easily decomposed, and for this reason, they are very suitable for environmental applications, especially bioremediation. The aim of this study was bioremediation and investigation of the absorption kinetics of heavy metals and Rhodamine B from water by biosurfactant produced from Pseudomonas aeruginosa bacteria.
Material and MethodologyIn this study, a biosurfactant-producing bacterium that was isolated and identified as Pseudomonas aeruginosa HAK02 from the Kahrizak waste site in the south of Tehran was used to produce a surface bioactive agent to remove pollutants. Zeta potential test was performed to detect the load of biosurfactant produced and used in bioremediation. The amount of color removal was done by UV device and the removal of heavy metals was done by ICP_AES analysis.
FindingsDue to the negative charge of the substance, it was used to remove heavy metals and Rhodamine B cationic dye. Biosurfactant produced with Pseudomonas aeruginosa was able to remove 95% rhodamine B, 43% Cd2+, and 35% Co2+. Examining the kinetic model of absorption showed that better correlation with pseudo-second order kinetic model.
Discussion and ConclusionBioremediation using surfactants to remove heavy metals and dyeing is a fast and environmentally friendly method. This sample is very suitable for the removal of Rhodamine B and has the moderate ability for heavy metals Cd2+ and Co2+.
Keywords: Bioremediation, Bio-surfactant, Cadmium, Cobalt, Kinetic model of absorption, Rhodamine B -
BACKGROUND AND OBJECTIVES
The Municipal Park of Areguais is located in the Central Department of Paraguay. Part of this Park is within the area of influence of Ypakarai Lake, which is widely recognized by vacationersfor its natural spaces. Despite being one of the most representative ecological reserves in the country, annual waterquality reports indicate the presence of a high content of pollutants; mainly nitrogen, phosphorus, andfecalcolifoirs, among others. These conditions promote the proliferation of cyanobacteria which consume the available oxygenand compromise the reserve’s flora and fauna. Following several laboratory tests, the present work has the objective of evaluating the impact of thepark’s recently constructed wetland’son the treatment of residual waters.There are several parameters evaluated in thisstudy of final wastewater discharge disposal treatmentthrough a constructed wetland of horizontal flow with Typhadomingensis. The objective is to develop an adequate system for the treatment of residual waters that can be replicated in places with similar conditions.
METHODSThe evaluation consisted of analyzing the residual water and finding the removal percentage for each of the following parameters such as chemical oxygen demand; biochemical oxygen demand; total phosphorus; total nitrogen; fecal coliforms; hydrogen potential; and temperature. The quality of the treated water was determined by comparing it with the limits established in Article 7 of Resolution Number 222/02 of the Environment Secretary for effluents. The results demonstrate that this system is aviableoption for the removal of fecal coliforms and nutrients such as phosphorus and nitrogen.
FINDINGSIn terms of the quality of the treated water, the parameters studied are within the limits,established by Resolution Number 222/02of the Environment Secretary for Class 2 waters, for water to be discharged into the receiving body. The results obtainedwere: 88.9 percent fecal coliform removal; 84.9 percent total nitrogen; 73.3 percentchemical oxygen demand; 61.4 percent biochemical oxygen demand; and 14.2 percent Total Phosphorus. Considering Resolution 222/02, the biochemical oxygen demand, Total Nitrogen, and Total Phosphorus were outside the admissible limits.
CONCLUSIONIt is very feasible for wastewater generated in public parks to be treated through the construction of sub-surface flowwetlands.This study confirms that the treated wastewater is within the establishedlimitsfor all the parameters: temperature, hydrogen potential, biochemical oxygen demand, chemical oxygen demand, total nitrogen, total phosphorus, and fecal coliforms. This model of water treatment can be easily adopted.
Keywords: Bioremediation, Black water, Constructed wetlands, Typha domingensis -
Pollution, Volume:9 Issue: 3, Summer 2023, PP 1128 -1139Fungi are successful microorganisms in the bioremediation of environmental pollution. So, this study aimed to determine the potential of Trichoderma tomentosum to remediate cadmium, lead, and nickel contaminations from potato dextrose agar (PDA) and potato dextrose broth (PDB) media. Growth rates, toxicity tolerance sporulation, bio-sorption capacity, and bio-sorption efficiency of the fungus were evaluated under different concentrations of CdCl2, Pb(NO3)2, and NiCl2. The findings demonstrated that the growth rate of the fungus differed depending on concentration, metal type, and medium. More metals in PDA medium induced more inhibition on fungus growth rates; however, the rate was independent from the heavy metals concentrations in PDB medium. Cadmium was the most toxic metal tested against T. tomentosum, with a 72h LC50 of 37 ppm. It was about 3.16 and 4.24 times as toxic as nickel and lead, respectively. In the control condition, sporulation of the fungus began at 72 hours, but under the heavy metals, it began at 168, 168, and 192 hours, respectively, for Pb, Ni, and Cd. Both the bio-sorption capacity and efficacy of the fungus were significantly enhanced by an increase in metal content and the highest values were obtained at 200 ppm of the salts. The heavy metals total bio-sorption capacity order was Ni < Cd < Pb in the aqueous medium. The conclusion was that T. tomentosum has a greater potential for the biosorption of heavy metals; hence, the fungus may be employed for the bioremediation of heavy metals from polluted sites, particularly wastewater and industrial influents.Keywords: bioremediation, fungi, Heavy metals, Pollution
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Pollution, Volume:9 Issue: 3, Summer 2023, PP 1028 -1048Anthropogenic activities have polluted soil and aquatic ecosystems by introducing harmful heavy metals (HMs) such as cadmium, copper, mercury, lead, manganese, nickel, zinc, and others. These HMs lead to serious health conditions in humans like cancer, skin lesions, birth defects, liver and kidney damage, and mental retardation leading to other disabilities. Conventional methods of HM remediation of contaminated soil and water include physical, chemical, biological, and integrated methods. The use of physical and chemical methods, in isolation, has been reduced in practice, owing to their negative impacts, however, work on suitable integrated approaches, and the use of organisms for HM remediation has been in steady progress since past few decades. These approaches have proved to be eco-friendly, cost-effective, and show reduced negative impacts on the environment and biota. However, there is consistent increase in anthropogenic contribution to this problem, so, to keep pace with it, more recently work is in advancement on exploiting the biological system to increase the efficiency of bioremediation, using the latest technologies such as genetic engineering and nanotechnology. This paper provides an overview of the current methods deployed to address this problem, developments made in this field in past few decades, and evokes a research thrust that might lead to novel remediation approaches in the future.Keywords: bioremediation, gems, microbes, nanobioremediation, Phytoremediation
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Pollution, Volume:9 Issue: 3, Summer 2023, PP 1059 -1073Heavy metal contamination poses grave risks to all kinds of life. The fastest growing automotive, electroplating, and battery industries release the most common heavy metal, Nickel, into the environment, which has lethal impacts on human health. Our research aims to find Ni-resistant bacteria in the metal-contaminated soil that have a great potential for removing Ni from the environment. Attempts have been made to extract and characterize Ni-resistant bacteria from automobile and electroplating industry waste-contaminated soil using serial dilution, streak plating, and various morphological, biochemical, and genetic techniques. The maximum tolerable concentration of Ni and other heavy elements, such as cadmium, lead, and aluminium for the selected isolate, was investigated using the UV-Vis spectrophotometric method. Additionally, the bacterial strain's ability to remove Ni was assessed using an atomic absorption spectrophotometer. The current research reveals a novel strain of Kluyvera cryocrescens that could withstand Ni, Cd, Pb, Al, and combinations of these heavy metals. The maximum tolerance concentration of K. cryocrescens M7 for Ni, Cd, Pb, and Al was found to be 150 ppm, 200 ppm, 1000 ppm, and 150 ppm, respectively. Additionally, it was also observed that the bacterial strain could remove Ni by 29.57%, 35.36%, 48.41%, 46.91%, and 44.88% after 12, 24, 48, 72, and 96 hours, respectively. The strain has also exhibited resistance to vancomycin, ampicillin, carbenicillin, and streptomycin. This research discovered a novel bacterial strain, K. cryocrescens M7 that may be beneficial for removing heavy metals, particularly Ni, from metal-contaminated soil.Keywords: Antibiotic resistance, bioremediation, Kluyvera cryocrescens, Metal removal, Nickel resistance
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Pollution, Volume:8 Issue: 4, Autumn 2022, PP 1418 -1433Textile effluents are highly colored for synthetic dyes, cause significant water pollution due to high pH, TDS, EC, BOD, and COD content, and are harmful to aquatic species. Among different treatment processes, biological treatment process is considered as a promising approach. In this investigation, a mixed aerobic bacterial consortium was used for the treatment of wastewater. In addition, the fenton process with a normal sand filter was used for treatment and compared with the biological method. The mean values of BOD, COD, TDS, EC, DO, and pH in the raw wastewater indicated that the effluent was highly contaminated according to Bangladesh standard (ECR, 1997). Both the biological treatment process and fenton process separately showed promising removal of pollution load. The aerobic mixed bacterial consortium reduced TDS (66.67%), EC (60%), BOD (91.67%), and COD (85.45%) and fenton process reduced TDS (74.71%), EC (55.11%), BOD (88.33%), and COD (83.63%) compared to the raw effluent bacterial consortium simultaneously degraded dyes and decolorized the wastewater from dark deep green to transparent. Color removal for the mixed aerobic bacterial process after 72 hours of aeration was 58.57% and for the fenton process with a normal sand filter was 80%. BOD and COD removal percentages for aerobic mixed bacterial consortium showed higher removal efficiency than the fenton process with a normal sand filter. Though 92 hours of aeration showed the maximum satisfactory result, aeration time could be reduced to 72 hours which also satisfied the Bangladeshi standard (ECR, 1997).Keywords: Textile effluents, bioremediation, advance oxidation process, coagulation-flocculation, microbes
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جذب زیستی فلز مس توسط باکتری های جنس Ochrobactrum جداسازی شده از رسوبات دریایی خور موسی در خلیج فارسآلودگی دریاها توسط فلزات سنگین یکی از عوارض اجتناب ناپذیر صنعتی شدن و توسعه ی جوامع بشری است. تحقیق حاضر به منظور جداسازی و شناسایی باکتری های مقاوم به مس و قابلیت آن ها در جذب زیستی مس انجام شد. نمونه های رسوب از 3 ایستگاه در خور موسی جمع آوری شدند و پس از کشت در محیط حاوی غلظت های مختلف مس، دو باکتری مقاوم به غلظت 100 میلی گرم در لیتر مس جداسازی شدند و توسط تست های بیوشیمیایی و آنالیز 16S rRNA شناسایی گردیدند. عملکرد این باکتری ها در جذب مس در محیط واجد این فلز بررسی شد. هر دو باکتری گرم منفی، اکسیداز- کاتالاز مثبت و متعلق به جنس Ochrobactrum بودند و تنها در واکنش به تست های بیوشیمیایی PD (phenylalanine deaminase)، NaCl و اوره تفاوت معنی داری نشان دادند. سنجش جذب زیستی غلظت های متفاوت مس نشان داد که هر دو سویه بیش از نیمی از فلز مس را در مدت زمان 150 دقیقه در تمامی غلظت ها جذب کردند. عملکرد هر دو سویه در غلظت 50 میلی گرم در لیتر تقریبا مشابه بود. زیرا سویه های O. tritici strain AN4 و O. anthropi strain YX0703 به ترتیب 73/5% و 72/6% مس را حذف کردند. اما در غلظت 200 میلی گرم در لیتر، سویه O. anthropi strain YX0703 درصد جذب بالاتری را نشان داد و در عرض 150 دقیقه میزان مس را از 200 میلی گرم در لیتر به 2/06±72/08 میلی گرم در لیتر کاهش داد. نتایج مطالعه حاضر نشان داد که این دو باکتری دارای قابلیت بالایی در حذف فلز مس هستند و می توان از پتانسیل این گروه از باکتری ها در کاهش آلودگی فلزات سنگین در خلیج فارس استفاده نمود.کلید واژگان: سویه باکتری, تست های بیوشیمیایی, فلزات سنگین, کاهش زیستیThe pollution of the marine ecosystems with heavy metals is one of the serious effect of industrialization and development of human society. For exploitation of marine sources and constant development, it’s necessary to invent modern methods for removal of heavy metal pollution. In present study to assess the possibility of removing copper, we aimed to investigate isolation and identification of resistant bacteria and determine their potential to adsorb copper through biosorption. Sediment samples were collected from 3 stations at Khor Mousa and cultured on nutrient agar medium containing different concentrations of copper. Two copper resistance (100 ppm) bacterial strains were isolated and identified through biochemical tests and 16S rRNA analysis. Also bacterial behaviors in adsorbtion of copper were studied. Both strains belonged to Ochrobactrum genus and were gram-negative, catalase and oxidase positive. Different characteristics of bacteria were observed in PD (phenylalanine deaminase), NaCl and Ureas tests. Biosorption measurements showed that strains could adsorb more than half of copper in various concentrations in 150 minutes. The bacterial behaviors were the same in 50 ppm concentration. %73.5 and %72.6 of copper were adsorbed by strains O. tritici strain AN4 and O. anthropi strain YX0703 respectively. However, O. anthropi strain YX0703 reduced the copper concentration from 200 ppm to 72.08 ppm in 150 minutes and showed the highest copper biosorption. The results of present investigation showed that both mentioned bacteria have high ability to remove copper and we can use the potential of this group of bacteria for removal of heavy metal pollution in Persian Gulf.Keywords: Bacterial Strains, Biochemical tests, heavy metals, Bioremediation
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زمینه و هدف
در دهههای اخیر، آلودگی محیطزیست، بهویژه خاک به فلزهای سمی در سطح جهانی افزایش چشمگیری داشته است. ورود فلزهای سمی به خاک از منابع مختلف، تهدیدی همیشگی و جدی برای سلامت گیاهان، جانوران و جوامع انسانی است. زیستپالایی با بهکارگیری میکروارگانیسمهای مفید خاکزی باعث افزایش راندمان پالایش مناطق آلوده به فلز میگردد و جایگزین مناسبی برای روشهای پالایش فیزیکوشیمیایی شناختهشده میباشد.
روش بررسیقارچهای آربوسکولار مایکوریزا (AM) در اکوسیستمهای مختلف دنیا از جمله در خاکهای آلوده به فلزهای سمی حضور دارند. این قارچها توسط مکانیسمهای مختلفی فلزهای سمی را در اندامهای قارچی درون و برون ریشهای غیرپویا کرده و علاوه بر کاهش اثر سمی فلزها بر گیاه میزبان، از ورود آن به زنجیرههای غذایی بالاتر ممانعت بهعمل میآورند. مقاله حاضر، به نقش گلومالین بهعنوان مولکول مهم دیواره سلولی اسپور و هیفهای قارچ AM در خاکهای آلوده به فلزات سمی پرداخته است.
یافته هانتایج نشان داد، گلومالین بهعنوان محصول اختصاصی قارچهای AM، در نقش یک پروتیین شوک حرارتی و نیز ترکیب عمده و اصلی در دیواره هیف و اسپورها حضور دارد.
نتیجه گیریگلومالین از طریق کاهش خطر سمیت و قابلیت دسترسی زیستی فلزها برای گیاهان و سایر موجودات، در حفظ و ارتقای سلامت خاک نقش مهم و کلیدی ایفا میکند.
کلید واژگان: سلامت خاک, زیست پالایی, کمپلکس گلومالین-فلز, همزیستی مایکوریزیAim and scopeIn the last few decades, contamination of the environment especially the soil by toxic metals has been increased extremely at worldwide. Entrance of toxic metals into the soil from various sources is a constant and serious threat to the health of plants, animals and human societies. Bioremediation by using of the beneficial soil microorganisms improves the remediation efficiency of the metal contaminated areas and is a suitable alternative method for substitution of current physico-chemical strategies.
MethodologyArbuscular mycorrhizal (AM) fungi are found in virtually all ecosystems worldwide, including in soil contaminated with toxic metals. AM fungi sequestrate toxic metals at fungal intra- and extracellular structures by different mechanisms, so in addition to reduce their toxic effects on host plant prevent from their entrance in the food chain. This study has been addressed the role of glomalin as an important molecule of the cell wall of AM fungi spores and hyphae in soils contaminated by toxic metals.
FindingThe results showed that glomalin as a specific product of AM fungi, is present in the role of a heat shock protein as well a critical and main component of spores and hyphal cell wall.
ConclusionGlomalin plays an essential and key role in maintaining and improving the soil health by reducing toxicity and availability of metals for symbiotic partner of AM fungi and other organisms.
Keywords: Soil health, Bioremediation, Glomalin-metal complex, Mycorrhizal symbiosis -
سابقه و هدف
برخی از فرآیندهای صنعتی، معدن کاری و استفاده از کودهای شیمیایی و قارچ کش ها در کشاورزی امروزی، منجر به رها سازی فلزات سنگین و همچنین افزایش شوری خاک و آب ها می شوند به گونه ای که افزایش سطح جهانی آلودگی به فلزات روند نگران کننده ای به خود گرفته است. از طرفی وجود یک بیلیون هکتار زمین های شور در سراسر کره زمین که تنها با پالایش فیزیکی، شیمیایی و زیستی قابل استفاده در کشاورزی می باشند، تهدیدی برای امنیت غذایی محسوب می شوند. وجود معادن بسیار در دنیا وایران و در نتیجه فرسایش خاک و انتشار غبار از سدهای باطله موضوعی نگران کننده است. پتانسیل سیانوباکترها در ایجاد پوشش بیولوژیک خاک، جذب فلزات سنگین و شوری خاک ها نسبت به روش های فیزیک وشیمیایی رایج، از جنبه کاربردی و اقتصادی بودن در حال بررسی می باشد.
مواد و روش هادر کارخانه طلای موته در نتیجه عملیات استخراج طلا به وسیله سیانوراسیون، روزانه پساب و رسوب خاک باطله به صورت گسترده ای حاصل می شود. در مهرماه 97 جمع آوری نمونه های خاک باطله از معدن طلا انجام شد. نمونه های خاک باطله معدن جهت شناسایی و رشد ریزجلبک ها، در محیط کشت اختصاصی جلبک ها کشت داده شد. قبل و بعد از قرارگیری 5 گرم خاک باطله معدن در معرض 5/0 گرم زیست توده سیانوباکتری ها، عناصر سدیم، پتاسیم و فلزات سنگین سرب و کادمیوم خاک باطله اندازه گیری شد و داده ها مورد آنالیز آماری قرار گرفت.
نتایج و بحثپس از کشت خاک معدن، سیانوباکترهای Oscillatoria tenuis C.Agardh ex Gomont ،tenue Gomont Phormidium، Lyngbya aestuarii Liebman ex Gomont و جلبک سبز Scenedesmus obliquus (Turpin) Kützing شناسایی شدند. نتایج کشت سیانوباکتری های رشته ای روی خاک های باطله معدن طلا نشان می دهد که این ریزموجودات فتوسنتز کننده می توانند به راحتی روی خاک های باطله آلوده معدن رشد کنند و پوشش بیولوژیک سطحی قابل ملاحظه ای را ایجاد کنند. همچنین نتایج نشان داد که O. tenuis، عنصر سدیم را بطور معنی داری کاهش داده است و P. tenue بیشترین میزان جذب سرب را داشته است و احتمالا با خالص کردن این دو گونه و استفاده جهت رشد روی خاک باطله معدن طلای موته می توان معدن را از شوری و عناصر سنگین به مقدار زیادی پاک سازی و همچنین از فرسایش خاک و انتشار غبار از سدهای باطله جلوگیری نمود.
نتیجه گیریبا توجه به وجود تعداد فراوان معادن مختلف طلا در دنیا و ایران، استحصال این فلز گران بها به طور وسیعی با استفاده از سیانور صورت گرفته که بدلیل سمیت بالا دارای اثرات سوء زیست محیطی زیادی می باشد. انتشار غبار سیانور از سد باطله می تواند سلامتی موجودات زنده نزدیک به آن از جمله کارگران معدن، گیاهان و حتی ریزجلبک های خاک زی را تحت تاثیر قرار دهد. با زیست پالایی خاک معادن می توان فرآیند احیای زیستی را با سرعت بیشتری انجام داد. نتایج کار محققان معادن طلای آفریقای جنوبی نشان می دهد که سیانوباکتری ها، پوشش بیولوژیکی سطحی قابل ملاحظه ای را روی خاک باطله ایجاد می کنند که با نتایج حاصل از این تحقیق مبنی بر رشد Phormidium سیانوباکتر مقاوم معدن طلای موته روی باطله ها و ایجاد پوشش بیولوژیک گسترده روی خاک مطابقت دارد.
کلید واژگان: Phormidium, فلزات سنگین, زیست پالایی, معدن طلای موتهIntroductionNatural and human activities lead to soil degradation and soil salinization. In the last two centuries, world metal pollution level has increased extremely. Presence of some heavy metals in aquatic ecosystems is a constant threat to the health of human societies. The decrease of farmlands threatens food security. There are approximately 1 billion ha salt-affected soils all over of world, which can be made available resources after chemical, physical and biological remediation. Existence of many mines in the world and also in Iran and as a result, soil erosion and dust release from waste dams is matter of concern. Bioremediation using biological agents to detoxify and degradation of environmental pollutants- provides a suitable alternative method for substitution of current heavy metals removal strategies.
Material and methodsDaily as a result of extraction operations by cyanidation in Mouteh gold mining complex, waste water and waste soil sediment widely achieved. In this study soil samples were collected from Mouteh Goldmine tailing damp soil in September 2018. The collected soil samples were cultured in BG11 medium and incubated for microalgae identification and biomass production. Also 5 gr soil inoculated with 0.5 gr biomass and the physico-chemical characteristics of the soil including pH, Na+ and K+, Pb and Cd, before and after the inoculation of soil with Cyanobacteria were determined.
Results and discussionin this study, cyanobacteria Phormidium tenue Gomont, Osillatoria tenuis C.Agardh ex Gomont, Lyngbya aestuarii Liebman ex Gomont and green Algae, Scenedesmus obliquus (Turpin) Kützing were identified. Culture results of filamentous cyanobacteria on goldmine tailing damp soil showed that cyanobacteria can grow easily and produced a surface crust significantly. The results of the Physical analysis of the soil samples showed that O. tenuis decreased Na as a salinity element, and P. tenue absorbed high amounts of Pb as a heavy metal pollution element. Probably by using Mouteh Goldmine soil cyanobacteria, Osillatoria tenuis and Phormidium tenue for mine tailingdamp soil bioremediation, could play mine recovery from salinity and heavy metals and also preventing soil erosion and dust release from waste dams.
ConclusionGenerally, it can be concluded thatdue to the existence of many goldmines in the word and Iran, daily as a result of extraction operations by cyanidation and high toxicity of cyanide, environmental adverse effects are observed. Dust release from waste dams can be affected health of living organisms like mining workers, plants and even soil microalgae which are close to the mine. By soil goldmine bioremediation can be processed biological resuscitation faster. The research results of African goldmine researchers on soil samples showed that cyanobacteria produced a surface crust significantly, that with the results of this research based on the growth of Phormidium tenue in the mine and producing a surface crust is consistent.
Keywords: Phormidium, Heavy Metal, Bioremediation, Mouteh Goldmine -
آلایندههای هیدروکربنهای نفتی، از لحاظ تجزیه و مقابله جزء ترکیبهای سخت بوده و به عنوان آلایندههای آلی پایدار و مهم طبقه بندی میشوند. این آلایندهها اثرات نامطلوبی بر سلامت انسان و محیط پیرامونش دارند و مقابله با آلودگی محیط زیست ناشی از آنها مسئله مهمی برای جهان و جوامع بشری است. هرچند پاکسازی این آلایندهها از محیط زیست یک مشکل اساسی است، اما تجزیه بیولوژیکی که از فعالیت بیولوژیکی طبیعی و تجزیه میکروبی استفاده میکند، یک روش سازگار با محیط زیست و اقتصادی برای کنترل این نوع آلودگیها است و در پاکسازی محیطهای آلوده با هیدروکربنهای نفتی به یک روش اصلی تبدیل شده است. پژوهش حاضر یک بررسی جامع، بههنگام و کارآمد درباره تصفیه زیستی آلایندههای هیدروکربنی نفت خام با در نظر گرفتن دگرگونی و تغییرات هیدروکربنها توسط میکروارگانیسمها با تمرکز ویژه بر بینشهای جدید به وجود آمده در چند سال اخیر را ارایه میدهد. همچنین متابولیسم هیدروکربنها در میکروارگانیسمها با مرور تحقیقات ارایه شده در چند سال اخیر تشریح شده است. نتایج مطالعه حاضر به خوبی نشان میدهد آلایندههای هیدروکربنهای نفتی با استفاده از برخی میکروارگانیسمها مانند اولیوفیلیک، تا حد قابل قبولی زیستتجزیهپذیر بوده و حذف آنها با این روش مقرون به صرفه و اقتصادی است، همچنین تجزیه بیولوژیکی میکروبی آلایندههای هیدروکربن نفتی از فعالیتهای کاتالیزوری آنزیمی میکروارگانیسمها استفاده میکند و میزان تخریب آلایندهها را تا چندین برابر روشهای سنتی افزایش دهد.
کلید واژگان: تخریب میکروبی, آلاینده های هیدروکربنی نفتی, تجزیه بیولوژیکی, پالایش زیستی2676-3060, Volume:6 Issue: 10, 2022, PP 135 -150Petroleum hydrocarbon pollutants are one of the hardest compounds in terms of decomposition and control and classified as stable and important organic pollutants that have adverse effects on human health and the environment and combating environmental pollution caused by them is an important issue for the world and human societies. Although the removal of these pollutants from the environment is a major problem, biodegradation (which uses natural microbial biodegradation activity) is an ecofriendly and economical approach to control these types of contaminants and has become a pivotal method of cleaning up environments contaminated with petroleum hydrocarbons. The present study provides a comprehensive, uptodate and efficient review of the bioremediation of petroleum hydrocarbon pollutants, taking into account the hydrocarbon alterations in microorganisms with a particular focus on the new insights gained in recent years. Also, the metabolism of hydrocarbons in microorganisms has been described by reviewing research presented in recent years. The results of studies show well that petroleum hydrocarbon pollutants are biodegradable using some microorganisms such as oleophilic and their removal by this method is cost-effective and economical. Microbial biodegradation of petroleum hydrocarbon contaminants uses the enzymatic catalytic activities of microorganisms to increase the degradation of contaminants several times more than traditional methods
Keywords: Microbial degradation, Petroleum hydrocarbon pollutants, Biodegradation, Bioremediation -
زمینه و هدف
پالایش زیستی روشی است که در آن از توانایی ریزجانداران جهت افزایش میزان و سرعت تجزیه آلاینده ها و درنتیجه کاهش آلودگی های محیط زیست استفاده می گردد. رطوبت و دما از عوامل محیطی اصلی تاثیرگذار بر رشد و فعالیت ریزجانداران و بالطبع بر کارآیی تجزیه زیستی آلاینده های آلی می باشند.
روش بررسیبه منظور بررسی تاثیر این دو فاکتور یک آزمایش فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار انجام شد. فاکتورهای مورد بررسی شامل؛ رطوبت در سه سطح (30%، 55% و 80% ظرفیت مزرعه)، دما در سه سطح (25، 30 و 35 درجه سانتی گراد) و تلقیح با باکتری در دو سطح (تلقیح شده با باکتری سودوموناس پوتیدا و بدون تلقیح با باکتری) بودند.
یافته هانتایج حاصل از تحقیق نشان داد که بیش ترین میزان تجزیه زیستی نفت خام در تیمار دارای شرایط رطوبت 55% ظرفیت مزرعه، دمای 30 درجه سانتی گراد و تلقیح با سودوموناس پوتیدا مشاهده شد که معادل 8/92% و کم ترین تجزیه در تیمار رطوبت 30% ظرفیت مزرعه، دمای 30 درجه سانتی گراد و بدون تلقیح با باکتری معادل 3/42% بود.
بحث و نتیجه گیریاین نتایج نشان می دهد که بهینه سازی عوامل محیطی در زیست پالایی می تواند سبب افزایش کارآیی پالایش تا 5/50 % شود.
کلید واژگان: آلودگی نفتی, زیست پالایی, شرایط محیطی, سودوموناس پوتیداBackground and ObjectiveBioremediation is an approach that exploits the ability of microorganisms to increase the rate and extent of degradation of pollutants and thereby removing pollutants from the environment. The moisture content and temperature are of the main environmental factors affecting growth and activity of microorganisms and accordingly affecting the efficiency of organic pollutant biodegradation.
MethodTo study the effect of these two factors a factorial experiment was carried out as completely randomized design with three replications. The factors were moisture in three levels (30%, 55% and 80% of Field Capacity), temperature in three levels (25, 30 and 35 degrees of Celsius) and inoculation with bacteria in two levels (with and without inoculation by Pseudomonas putida) which were triplicated.
FindingsThe results showed that highest biodegradation rate was observed in the treatment with the moisture content of 55% F.C, temperature of 30 degrees of Celsius and inoculation with Pseudomonas putida which was 92.8% and the lowest biodegradation rate was observed in the treatment with the moisture of 30% F.C, temperature of 30 degrees of Celsius and without inoculation which was 42.3%.
Discussion and ConclusionThese results shows that the optimization of the environmental conditions in bioremediation process may lead to 50.5% increase in the efficiency of removal.
Keywords: Crude Oil Contamination, Bioremediation, Environmental Conditions, Pseudomonas Putida -
The present study focused on the synergistic effects bioremediation of phenol in aqueous solution using combination of anaerobic mixed culture and Granular Activated Carbon (GAC) as a biological GAC (BGAC). Meanwhile, the effect of contact time and various phenol concentrations on adsorption and biosorption process investigated. The phenol concentration was analyzed using UV/Vis spectrophotometer. The morphology and structure of two adsorbents (GAC and BGAC) were characterized by FESEM and BET specific surface area analysis. The batch experiments using mixed bacterial culture, isolated from wood and paper factory wastewater, were adapted to high concentrations of phenol and employed in order to evaluate the tolerance and biosorption capability of microorganisms for phenol biodegradation. The synergetic effect of phenol removal using combination of GAC with an anaerobic biofilm indicated that the removal efficiency for concentration of 700, 800, and 1000 mg/l at initial stages increased to 4, 10, and 12%, respectively and while by increment of the retention time did not shown significant impact on the removal efficiency. These results conducted both desorption of adsorbates due to biotransformation in the aqueous solution and direct assimilation of adsorbates on GAC by the microorganism’s biofilm. The adsorption data were fitted with pseudo-first-order and pseudo-second-order models and it was found that the pseudo-second-order model explains the adsorption kinetics more efficiently. The compatibility of the Freundlich and Langmuir adsorption models to equilibrium data were investigated. In fact, the Langmuir isotherm was found to be the best fitting isotherm.Keywords: Anaerobic culture, Bioremediation, Equilibrium Isotherms, Granular Activated Carbon, Kinetic Models, Phenol
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Pollution, Volume:6 Issue: 3, Summer 2020, PP 627 -635Butachlor (BUT) is a chloroacetanilide herbicide widely applied to rice paddies to control annual grass and broad-leaf weeds. A BUT-degrading bacterial strain (PK) was isolated from paddy soils. Biochemical and 16S rRNA sequencing characteristics confirmed the strain as Pseudomonas aeruginosa (99% resemblance). The isolate dissipated BUT (100 μg/mL) in an M9 liquid medium with a rate of 0.5 ± 0.03 day-1 and DT50 and DT90 of 1.38 ± 0.10 days and 4.58 ± 0.32 days, respectively. Soil dissipation of BUT was investigated under flooded conditions. In sterile soils, the isolate increased the dissipation of BUT (200 μg/g) (DT50 = 12.38 ± 1.83 days, DT90 = 41.12 ± 6.09 days, k = 0.06 ± 0.01 day-1) compared to sterile non-inoculated samples (DT50 = 26.87 ± 2.82 days, DT90 = 89.25 ± 9.36 days, k = 0.03 ± 0.00 day-1). In non-inoculated non-sterile soil experiments, the dissipation of BUT was faster (DT50 = 15.17 ± 2.11 days, DT90 = 50.38 ± 7.02 days, k = 0.05 ± 0.00 day-1) compared to non-inoculated sterile ones, and inoculating the isolate accelerated the removal of BUT in non-sterile soils significantly (DT50 = 8.03 ± 1.20 days, DT90 = 26.68 ± 3.97 days, k = 0.09 ± 0.01 day-1). BUT inhibited soil respiration (SR) initially for 5 days, followed by an increase until day 20. The increase in SR was more pronounced in the co-presence of BUT and the isolate. The results of this research suggest P. aeruginosa PK as a suitable candidate for BUT bioremediation.Keywords: Bacterial isolate, bioremediation, Pseudomonas aeruginosa, Paddy, Soil respiration
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The abilities of three indigenous bacteria for bioremediation of cadmium contaminated soils collected from Agbabu Farm Settlement close to mining sites in Ondo state, Nigeria was studied to provide helpful information for soils remediation and soils health management in this sub-region for Millennium Development Goals accomplishment. Bacillus subtilis, Proteus mirabilis, and Escherichia coli isolated from the soils were inoculated into different soil samples conditioned with optimized factors determined from the first phase experiments. The conditioned samples were experimented for residual cadmium concentration with time in days using Atomic Absorption Spectrophotometer. The soil cadmium attenuation from the initial concentration of 70.21 mg/kg to below the maximum allowable of 3 mg/kg was hard for the organisms. Bacillus subtilis performed correction at time 35 days with an efficiency of 96.10 % and residual concentration of 2.74 mg/kg. Proteus mirabilis and Escherichia coli with respective, high efficiencies of 85.05% and 79.35% failed. The removal rate capacities were -0.131d-1 for B. subtilis; -0.111d-1 for P. mirabilis; -0.105d-1 for E. coli. Four kinetic models fitted described the experimental data well. The models assessment revealed the removals to be transport controlled as diffusion process was the rate-controlling step.
Keywords: Bioremediation, Biosorption, Cadmium Removal, Contaminated Soil, Diffusion, heavy metals
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