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جستجوی مقالات مرتبط با کلیدواژه « bioremediation » در نشریات گروه « محیط زیست »

تکرار جستجوی کلیدواژه «bioremediation» در نشریات گروه «علوم پایه»
  • M. Iqhrammullah, S. Saudah, M. Monalisa, F. Fahrurrozi, S.A. Akbar *, S.S. Lubis
    BACKGROUND AND OBJECTIVES
    The presence of heavy metals, specifically cadmium, in the environment poses significant threats to both ecological systems and human health. However, microalgae have shown potential in addressing this issue through their ability to absorb cadmium and produce valuable biomass, making them a promising solution for bioremediation. Among the various microalgae species, Chlorella vulgaris stands out as a suitable candidate due to its potential for biodiesel production and its capacity to effectively absorb cadmium. Therefore, the main objective of this study is to assess the toxicity of cadmium on Chlorella vulgaris cells using network meta-analysis as a methodology.
    METHODS
    A comprehensive search was conducted on Scopus, Scilit, Google Scholar, and Web of Science to identify relevant studies published from 1 January 1990 to 16 January 2024. Only studies that reported the cell number of Chlorella vulgaris as a result of cadmium exposure were considered for inclusion. The collected data were then subjected to Bayesian frequentist network meta-analysis, utilizing standardized mean difference and a 95 percent confidence interval as measures of effect size. Additionally, a linear regression analysis was performed to examine the dose-dependent impact of cadmium toxicity.
    FINDINGS
    Dose-dependent toxic effects of cadmium on Chlorella vulgaris were evident (R-square of more than 0.90), particularly at a concentration of 1 part per million, deemed as the maximum tolerable threshold. Prolonged exposure revealed a concentration-dependent reduction in cell viability, suggesting potential lifespan shortening. A comparison of growth stimulants, gibberellic acid and brassinolide (standard means differences of 1.7 and 3.8, respectively), in mitigating cadmium toxicity indicated the latter superior effectiveness in sustaining microalgal survivability. The presence of high nitrogen and low phosphorous levels was found to be significantly associated with a reduction in Chlorella vulgaris cells due to cadmium exposure.
    CONCLUSION
    This research has provided conclusive proof of the harmful effects of cadmium on Chlorella vulgaris through the implementation of Bayesian frequentist network meta-analysis, offering valuable insights for environmental management practices. The findings reveal concentration-dependent effects of cadmium toxicity. The survivability of Chlorella vulgaris is determined by the compositions of macronutrients nitrogen and phosphorous. Comparative analyses highlight the superior protective effect of brassinolide over gibberellic acid in mitigating cadmium toxicity. Overall, the findings highlight the potential of Chlorella vulgaris in both bioremediation of heavy metals and biomass production.
    Keywords: Bioremediation, Cadmium, Chlorella Vulgaris, Growth, Heavy Metals}
  • I. Marzuki *, I. Pratama, R. Asaf, A. Athirah, K. Nisaa, N. Nurbaya, M. Muslimin, N. Nurhidayah, S. Suwardi, A. Sahrijanna, K. Kamaruddin
    BACKGROUND AND OBJECTIVES
    The quality and production volume of the cultivation of tiger prawn Penaeus monodon have decreased considerably in the last two decades. However, intensification and extensification efforts, including the application of cultivation technology through pond land recovery, have not produced expected results. Visible symptoms suggest potential issues with the cultivation water possibly originating from exposure to heavy metal pollutants. Therefore, this study aimed to remove heavy metal pollutants by using sponge symbiont bacteria bioremediators to increase the survival rate and quality of tiger prawn production. The achievements of this research are expected to contribute to the scientific development of environmental microbiology, bioremediation, and aquaculture pollution control.
    METHODS
    The study utilized Bacillus pumilus and Pseudomonas stutzeri bacteria. The water used for tiger prawn post-larvae cultivation was treated with these bioremediator bacteria. The water had copper and lead ion concentrations that were 20 times greater than the maximum threshold value. The physical and chemical characteristics and parameters, such as dissolved organic matter, nitrite, nitrate, and ammonia contents, of the cultivation water were monitored over a 30-day period. The specific growth rate in terms of weight and body length and the survival rate of the tiger shrimps were measured to evaluate the effect of the bioremediation process on the prawns. The concentrations of copper and lead ions in the cultivation water and within the body of the tiger shrimps were analyzed. The health of the tiger prawns was evaluated by observing signs of tissue damage.
    FINDINGS
    Among all the treatments, Treatment I with copper ion exposure had the highest average specific growth rate of the tiger prawns in terms of weight and body length, followed by Treatment II with lead ion exposure and Treatment III with a combination of both pollutants (the lowest). The intersection of copper and lead ion concentrations in the tiger prawns and cultivation media occurred in the cultivation period of 15–20 days. The use of Bacillus pumilus and Pseudomonas stutzeri bacteria as bioremediators effectively remediated the copper and lead pollutants at an average of 99.34 percent and 97.18 percent of the initial concentration, respectively. Despite the bioremediation efforts, the tiger shrimps exhibited symptoms of tissue damage in the head, tail, and shell. These symptoms included necrosis, myopathy, and infiltration, which are indicative of decreased cell function due to the presence of toxic agents.
    CONCLUSION
    Bioremediation with Bacillus pumilus and Pseudomonas stutzeri bacteria helped reduce the accumulation of heavy metal pollutants. However, negative effects on the health and growth of tiger prawns were still observed when the prawns were exposed to copper and lead ion concentrations below the allowed threshold value.
    Keywords: Bioremediation, Cultivation media, Heavy metal pollutants, Sponge symbiont bacteria, Tiger prawns}
  • Ibtesam Kadhum Khuder, Maha Abdul Ameer, Haider Alghanmi *
    The current study tested the ability of the aquatic plant Azolla pinnata to remove the pyrene and determine the effect of Pyrene on the pigments (chlorophyll a, b, and carotenoids) and antioxidant parameters (CAT, SOD, MDA and, ROS) in the plant. The plant was exposed to different concentrations of Pyrene (1,5,10,15,25, and 50 mg/l) for 14 days. The results showed that the high removal rate of pyrene reached 99% for 25 and 50 mg/l of pyrene on the 14th day of the experiment, while the lowest removal rate was 42.1% for 1 mg/l on the third day.The results showed the lowest values of total chlorophyll, chlorophyll a and carotenoids were 0.55, 0.25, and 0.433 μg/g recorded at 50 ppm respectively. While chlorophyll b recorded a low value of 0.32 μg/g at 25 mg/l, the highest concentration of these pigments was registered in the control group. Also, the results showed a gradual increase in antioxidant values compared to the control group, recording the highest values of 0.2873 and 1.04 U/g at 50 ppm and the lowest value of CAT and SOD at 5ppm, compared to the control group. Also, the study recorded a gradual increase in ROS and MDA values with increasing concentrations of pyrene, the highest value was 1.6367 and 14.4433 µmole/g for ROS and MDA at (50ppm), whereas the lowest value was recorded at 5 ppm compared to the control group. Statistical analysis at (p˂0.05) showed significant differences between all the interactions.
    Keywords: Azolla Pinnata, Bioremediation, Pahs, Pyrene}
  • Reagan Agbor *, Eno Asuquo, Ettah Ivon, Simon Ellen
    Environmental pollution has posed a major threat to terrestrial, aquatic, and marine ecosystems, thereby affecting microflora and micro-fauna populations. This study assessed the growth attributes of maize plants on crude oil-polluted soils amended with agro-wastes. Six kilograms each of composite soil sample was weighed and transferred into one hundred and fifty labeled plastic buckets with drainage holes for soil aeration and spiked with 300mls each of crude oil, allowing for 14 days of soil acclimatization. Soil amendments such as groundnut husks, cassava peels, empty fruit bunch of oil palm, and maize cob powder were applied and allowed for 90 days. Maize seeds were sowed, while periodic data were collected and subjected to a three-way ANOVA. The result obtained revealed that maize seeds grown on agro-wastes treated and pristine control soils show early seed germination than the crude oil-polluted control soil. The plant height obtained for GnH14P + MaC14P at 10% was the highest with a mean (of 152.81cm2), and the leaf area of the maize from soil treated with GnH14P + EFBOP14P at 10% had the highest mean (756cm2), the leaf length of maize from soil treated with GnH14P + CasP14P at 3%, 6%, and 10% was the highest with mean ranging (54-97 cm2) with no significant difference in mean values obtained. The stem girth, number of leaves, and leaf width were generally improved in the bio-remediated soils. The result for the yield performance of maize shows that the days to flowering were shortened in the bio-remediated soil compared to the prolonged flowering days observed in the crude-oil polluted control. The number of seeds per cob was high in the bio-remediated soils while no seed was obtained in the crude-oil-polluted control soils. It can be concluded that the ameliorated treatment with the agro-wastes improves the performance of maize plants in crude oil-polluted soils.
    Keywords: soil, hydrocarbons, maize, Agro-wastes, bioremediation}
  • مرضیه کلاه کج*

    آلودگی فلزات سنگین به دلیل پایداری بالای آنها در محیط یکی از نگرانی های اصلی اکوسیستم های آبی به شمار می رود. ایران با داشتن پهنه آبی قابل توجه از جمله خلیج فارس، دریای خزر و دریای عمان ، و وجود مخازن نفت و گاز در این پهنه های آبی و ساخت و سازه ای ساحلی، در معرض خطر آلودگی های فراوان قرار دارد. هر گونه تغییر در اکولوژی این پهنه های آبی ناشی از آلودگی خطرات جبران ناپذیری خواهد داشت. تاکنون مطالعات گسترده ای درمورد روش های پاکسازی محیط از فلزات سنگین صورت گرفته است. زیست پالایی یک اصطلاح کلی در جهت رفع آلودگی های زیست محیطی به وسیله فرآیندهای بیولوژیکی و میکروارگانیسم ها به خصوص باکتری ها، قارچ ها و مخمرها در خاک ها و آب های آلوده است که نسبت به روش های فیزیکو شیمیایی نظیر تبادل یونی و رسوب دهی شیمیایی، به دلیل اقتصادی بودن، بالا بودن سرعت نسبی فرآیند جذب، و عدم تولید لجن به عنوان یک روش مناسب و بهینه مطرح شده است. طی این مطالعه مروری به معرفی شاخص های مناسب زیست پالایی و مطالعات انجام شده در این خصوص در پهنه های آبی کشور پرداخته می شود.

    کلید واژگان: فلزات سنگین, زیست پالایی, میکروارگانیسم, محیط زیست}
    Marzieh Kolahkaj *
    Introduction

    In different regions of the Persian Gulf, oil pollution along with other urban, agricultural and industrial pollution has destroyed this valuable ecosystem and the valuable aquatic resources in it have been exposed to the risk of various corruptions and have threatened the aquatic populations in it. (Pourrang, 2005). The Caspian Sea is one of the unique lakes in the world due to its biological resources (plants and animals) and supply of protein materials, sturgeon fishing, and caviar production (Bazrafshan, 1374). In recent years, the increasing development of human activities in the countries bordering the Caspian Sea has changed the ecology of this sea. The Caspian Sea has oil and gas refineries and oil reserves in oil fields and exploration and drilling activities by five common countries, especially Azerbaijan and Kazakhstan. And the discharge of sewage and industrial effluents has caused the pollution of the sea (Varedi, 2015). Some microorganisms are present in the contaminated site, but for more effective remediation, the growth of the microorganism must be stimulated. Biostimulation is the process of adding nutrients, electron acceptors, and oxygen to stimulate bacteria in bioremediation. This is the process of optimizing the environmental conditions of the place of correction. Additives are usually added to subsurface layers through injection wells. Subsurface characteristics such as groundwater velocity, subsurface hydraulic conductivity, and subsurface lithology are essential for the development of a biological stimulation system. The natural microorganisms in the soil are responsible for the decomposition of pollutants. But biostimulation can be improved with biological additives. The quality of life on earth depends on the quality of the environment. Large amounts of organic and inorganic substances are released into the environment every year as a result of human activities. Contaminated lands are generally the result of industrial activities, the use, and disposal of hazardous substances, and such. It is now widely recognized that contaminated land is a potential threat to human health, and its continued discovery over the past 13 years has led to international efforts to remediate many sites, or as a response to health hazards.or environmental effects caused by pollution or to activate this place for use, to be developed again. Bioremediation is a general concept that includes all those processes and actions that take place to transform an environment, which is currently changedby pollutants to its original state (Adhikari, 2004; Gunasekaran, 2003). Also, bioremediation means the process of cleaning hazardous waste with microorganisms or plants, and it is the safest method of cleaning soil pollution.So far, there have been several studies on biological indicators (various plant and animal species as biological monitors) of heavy metals in both aquatic and terrestrial environments, which we can mention here. The use of biological information and biological control programs for water pollution for the management of ecosystems started at the beginning of the 1920s in the United States and then in a more serious way since 1948 all over the world. Today, many researchers from international organizations, including the EPA and the World Health Organization (WHO), introduced these indicators as the most appropriate indicators for environmental assessment and ecosystem management (Freund, 2007). Hilsenhoff's research, which started in 1979 with periodic sampling of the end of aquatic habitats and measurement of physical and chemical parameters, and finally using the biological coefficient formula; is considered a turning point in the biological evaluations of the environment (Hilsenhoff, 1988).Also, by examining the management of drilling waste, which is one of the new biological methods being developed in Iran, we will deal with it. The wastes of drilling operations are mainly the result of the washing and cooling activities of drilling machines. These wastes are generally divided into two groups in terms of physical phase: solids and liquids. In the new method invented in this collection, these wastes are managed separately. Solids are recycled by biological methods (composting) and liquids are recycled by three chemical methods, biological absorbents, and evaporation-condensation, depending on their initial quality. This new method first recycles water with the help of flow management and mixing, and then with different chemical, physical, and sometimes biological methods.The results of various surveys show that by measuring different pollutants in the plants of the same region, it is possible to understand the state of pollution of various compounds, including heavy metals (Lehndorff, 2009). In 2010, Bonanno and Gaddis in South Phragmites examined reed aquatic plants in terms of the accumulation of heavy metals cadmium, chromium, copper, mercury, manganese, nickel, lead, and zinc in the root, rhizome, stem and leaf tissues and concluded They found that the mentioned plant can be a monitor to control water pollution (Bonanno, 2010). Wild oysters can also be used to monitor pollution. In 2011, Victoria et al. investigated the concentration of heavy metals in the soft tissue of a species of wild oysters from the Spanish North Atlantic coast and stated that wild oysters are a benchmark for international evaluation. Besada, 2011). Also, in 2015, Phillips et al. tested the concentration of four heavy metals cadmium, copper, lead, and zinc in the tissue of three aquatic plants, and based on the results of the research they have conducted, these species are suitable for use as indicators of the presence and level of pollutants. Heavy metals are introduced into estuary water places (Phillips, 2015). In Iran, many types of research have been conducted so far, by examining their results, the potential of different species in the direction of bioremediation can be achieved, and we are now mentioning some of them. In 2013, during research, Azami et al. investigated the amount of total mercury in different tissues of three of the most important waterfowl species in the north of the country, including the great cormorant, the mallard, and the green duck, and compared the results with the international standard of the American Environmental Protection Organization. Global health and domestic standards are compared and finally, while warning consumers of waterfowl, especially vulnerable people, the results of their research are an achievement for managers to control and monitor metal pollution in Gamishan and Anzali wetlands, which are one of the most important habitats. The wintering birds are listed (Aazami, 2012).

    Conclusion

    Although bioremediation is not the answer to all pollution, it provides a method of removing pollution by enhancing biological processes and natural biological decomposition. Also, its application in each place must be determined and depends on the local mineralogy, hydrology, geology, and chemistry of that place. Therefore, with the development and understanding of microbial communities and their response to the natural environment and pollutants, expanding the knowledge of microbial genetics to increase the ability to reduce pollutants, and conducting proper tests of new bioremediation techniques that are cost-effective, this technology is an efficient and cost-effective way to purify water, contaminated land, and soil. Therefore, this method is being developed and improved to be able to cover more areas of pollution.

    Keywords: heavy metals, Bioremediation, Microorganism, Environment}
  • Reagan Bessong Agbor *, Ndem Eyogor Edu, Eno Ndarake Asuquo, Etta Akpang Ivon, Simon Alain Inah, Obase-Etta Bebia
    Bioremediation has become a trending and developing field in environmental restoration through the use of micro-organisms to utilize and reduced the concentration and toxicity of various chemical pollutants. This study is on bioremediation of hydrocarbon-polluted soils using some agricultural wastes. Ninety (90) plastic buckets were filled with 4kg each of the composite soil. The soil contained in the plastic buckets was spiked with 250ml crude oil, except in the unpolluted plastic buckets (0%) crude oil. The agro-wastes (plantain stem sap, bush mango peels, and fruited pumpkin husk powder) in single and combined forms were applied after 14 days soil pollution. The amendments were applied as follows: Pristine control (0% agro-wastes), crude-oil control (0% agro-wastes), 150g, 250g, and 350g of the agro-wastes. Soil samples were collected at 90 days for soil microbial counts and the total hydrocarbon content of the soil. Data collected were subjected to 2-way ANOVA. The result showed that the microbial population in the crude-oil polluted soil amended with different agricultural wastes significantly increased (p<0.05) the total heterotrophic and crude oil utilizing bacterial and fungal counts in the soils and the increase in microbial population result in a significant reduction in total hydrocarbon content (THC) of the soils. The reduction in the THC of the soil was treatment dependent. It is, therefore concluded that based on the efficiency of these agro-wastes in enhancing microbial degradation, further studies should be carried out on the enzyme activities and production of bio-surfactant from the wastes to shorten the degradation time.
    Keywords: Contamination, Amendment, bioremediation, fungi, Bacteria}
  • فاطمه دیبا، بابک مختارانی*، رضا پناهی
    زمینه و هدف

    یک روش جایگزین و سازگار با محیط زیست جهت پالایش محیط زیست از آلودگی ها، استفاده از مواد زیست فعال سطحی مشتق شده از میکروارگانیسم ها است. مزیت زیست فعال سطحی، زیست تخریب پذیری، سمیت پایین و اثر بخشی در افزایش تجزیه بیولوژیکی است. برخلاف سورفکتنت های شیمیایی، مواد فعال سطحی تولید شده از میکروب ها به آسانی تجزیه می شوند و به همین دلیل برای کاربردهای زیست محیطی به خصوص زیست پالایی بسیار مناسب اند. این پژوهش با هدف زیست پالایی و بررسی سینتیک جذب فلزات سنگین(کبالت و کادمیم) و رنگ رودامین B از آب توسط بیوسورفکتنت تولید شده از باکتری سودوموناس آیروژینوزا صورت گرفت.

    روش بررسی

    در این مطالعه از یک باکتری مولد بیوسورفکتنت که از سایت زباله کهریزک در جنوب تهران به عنوان سویه سودوموناس آیرژینوزا HAK02 جداسازی و شناسایی شده جهت تولید زیست فعال سطحی برای حذف آلاینده ها استفاده شد. تست زتا پتانسیل برای تشخیص بار بیوسورفکتنت تولید شده و استفاده در زیست پالایی انجام شد. میزان حذف رنگ توسط دستگاه UV و حذف فلزات سنگین به وسیله آنالیز ICP_AES انجام گرفت.

    یافته ها

    با توجه به بار منفی ماده برای حذف فلزات سنگین و رنگ کاتیونی رودامین B استفاده شد. بیوسورفکتنت تولید شده با سودوموناس آیرژینوزا قادر به حذف 95% رودامینB ، 43%Cd2+  و 35% Co2+ شد. بررسی مدل سینتیکی جذب نشان داد با مدل سینتیکی شبه درجه دوم تطبیق بهتری داشت.

    بحث و نتیجه گیری

    زیست پالایی با استفاده از زیست فعال سطحی جهت حذف فلزات سنگین و رنگبری روشی سریع و سازگار با محیط زیست می باشد. این نمونه جهت حذف رودامینB بسیار مناسب است و برای فلزات سنگین Cd2+ و Co2+ توانایی متوسط دارد.

    کلید واژگان: رودامین B, زیست پالایی, زیست فعال سطحی, کادمیوم, کبالت و مدل سینتیکی جذب}
    Fatemeh Diba, Babak Mokhtarani *, Reza Panahi
    Background and Objective

    An alternative and environmentally friendly method for purifying the environment from pollution is the use of biosurfactant derived from microorganisms. The advantage of biosurfactant is biodegradability, low toxicity, and effectiveness in increasing biological decomposition. Unlike chemical surfactants, surface active substances produced by microbes are easily decomposed, and for this reason, they are very suitable for environmental applications, especially bioremediation. The aim of this study was bioremediation and investigation of the absorption kinetics of heavy metals and Rhodamine B from water by biosurfactant produced from Pseudomonas aeruginosa bacteria.

    Material and Methodology

    In this study, a biosurfactant-producing bacterium that was isolated and identified as Pseudomonas aeruginosa HAK02 from the Kahrizak waste site in the south of Tehran was used to produce a surface bioactive agent to remove pollutants. Zeta potential test was performed to detect the load of biosurfactant produced and used in bioremediation. The amount of color removal was done by UV device and the removal of heavy metals was done by ICP_AES analysis.

    Findings

    Due to the negative charge of the substance, it was used to remove heavy metals and Rhodamine B cationic dye. Biosurfactant produced with Pseudomonas aeruginosa was able to remove 95% rhodamine B, 43% Cd2+, and 35% Co2+. Examining the kinetic model of absorption showed that better correlation with pseudo-second order kinetic model.

    Discussion and Conclusion

    Bioremediation using surfactants to remove heavy metals and dyeing is a fast and environmentally friendly method. This sample is very suitable for the removal of Rhodamine B and has the moderate ability for heavy metals Cd2+ and Co2+.

    Keywords: Bioremediation, Bio-surfactant, Cadmium, Cobalt, Kinetic model of absorption, Rhodamine B}
  • A.M. Vazquez, A. Samudio-Oggero *, H.D. Nakayama, I. Cantero-García
    BACKGROUND AND OBJECTIVES

    The Municipal Park of Areguais is located in the Central Department of Paraguay. Part of this Park is within the area of influence of Ypakarai Lake, which is widely recognized by vacationersfor its natural spaces. Despite being one of the most representative ecological reserves in the country, annual waterquality reports indicate the presence of a high content of pollutants; mainly nitrogen, phosphorus, andfecalcolifoirs, among others. These conditions promote the proliferation of cyanobacteria which consume the available oxygenand compromise the reserve’s flora and fauna. Following several laboratory tests, the present work has the objective of evaluating the impact of thepark’s recently constructed wetland’son the treatment of residual waters.There are several parameters evaluated in thisstudy of final wastewater discharge disposal treatmentthrough a constructed wetland of horizontal flow with Typhadomingensis. The objective is to develop an adequate system for the treatment of residual waters that can be replicated in places with similar conditions.

    METHODS

    The evaluation consisted of analyzing the residual water and finding the removal percentage for each of the following parameters such as chemical oxygen demand; biochemical oxygen demand; total phosphorus; total nitrogen; fecal coliforms; hydrogen potential; and temperature. The quality of the treated water was determined by comparing it with the limits established in Article 7 of Resolution Number 222/02 of the Environment Secretary for effluents. The results demonstrate that this system is aviableoption for the removal of fecal coliforms and nutrients such as phosphorus and nitrogen.

    FINDINGS

    In terms of the quality of the treated water, the parameters studied are within the limits,established by Resolution Number 222/02of the Environment Secretary for Class 2 waters, for water to be discharged into the receiving body. The results obtainedwere: 88.9 percent fecal coliform removal; 84.9 percent total nitrogen; 73.3 percentchemical oxygen demand; 61.4 percent biochemical oxygen demand; and 14.2 percent Total Phosphorus. Considering Resolution 222/02, the biochemical oxygen demand, Total Nitrogen, and Total Phosphorus were outside the admissible limits.

    CONCLUSION

    It is very feasible for wastewater generated in public parks to be treated through the construction of sub-surface flowwetlands.This study confirms that the treated wastewater is within the establishedlimitsfor all the parameters: temperature, hydrogen potential, biochemical oxygen demand, chemical oxygen demand, total nitrogen, total phosphorus, and fecal coliforms. This model of water treatment can be easily adopted.

    Keywords: Bioremediation, Black water, Constructed wetlands, Typha domingensis}
  • Samira Hosseinzadeh, Ali Asghar Aliloo *, Saleh Shahabivand, Mohammad Ghaderi
    Fungi are successful microorganisms in the bioremediation of environmental pollution. So, this study aimed to determine the potential of Trichoderma tomentosum to remediate cadmium, lead, and nickel contaminations from potato dextrose agar (PDA) and potato dextrose broth (PDB) media. Growth rates, toxicity tolerance sporulation, bio-sorption capacity, and bio-sorption efficiency of the fungus were evaluated under different concentrations of CdCl2, Pb(NO3)2, and NiCl2. The findings demonstrated that the growth rate of the fungus differed depending on concentration, metal type, and medium. More metals in PDA medium induced more inhibition on fungus growth rates; however, the rate was independent from the heavy metals concentrations in PDB medium. Cadmium was the most toxic metal tested against T. tomentosum, with a 72h LC50 of 37 ppm. It was about 3.16 and 4.24 times as toxic as nickel and lead, respectively. In the control condition, sporulation of the fungus began at 72 hours, but under the heavy metals, it began at 168, 168, and 192 hours, respectively, for Pb, Ni, and Cd. Both the bio-sorption capacity and efficacy of the fungus were significantly enhanced by an increase in metal content and the highest values were obtained at 200 ppm of the salts. The heavy metals total bio-sorption capacity order was Ni < Cd < Pb in the aqueous medium. The conclusion was that T. tomentosum has a greater potential for the biosorption of heavy metals; hence, the fungus may be employed for the bioremediation of heavy metals from polluted sites, particularly wastewater and industrial influents.
    Keywords: bioremediation, fungi, Heavy metals, Pollution}
  • Meena Yadav *, Poonam Sharma
    Anthropogenic activities have polluted soil and aquatic ecosystems by introducing harmful heavy metals (HMs) such as cadmium, copper, mercury, lead, manganese, nickel, zinc, and others. These HMs lead to serious health conditions in humans like cancer, skin lesions, birth defects, liver and kidney damage, and mental retardation leading to other disabilities. Conventional methods of HM remediation of contaminated soil and water include physical, chemical, biological, and integrated methods. The use of physical and chemical methods, in isolation, has been reduced in practice, owing to their negative impacts, however, work on suitable integrated approaches, and the use of organisms for HM remediation has been in steady progress since past few decades. These approaches have proved to be eco-friendly, cost-effective, and show reduced negative impacts on the environment and biota. However, there is consistent increase in anthropogenic contribution to this problem, so, to keep pace with it, more recently work is in advancement on exploiting the biological system to increase the efficiency of bioremediation, using the latest technologies such as genetic engineering and nanotechnology. This paper provides an overview of the current methods deployed to address this problem, developments made in this field in past few decades, and evokes a research thrust that might lead to novel remediation approaches in the future.
    Keywords: bioremediation, gems, microbes, nanobioremediation, Phytoremediation}
  • Heena Bisht, Narayan Kumar *
    Heavy metal contamination poses grave risks to all kinds of life. The fastest growing automotive, electroplating, and battery industries release the most common heavy metal, Nickel, into the environment, which has lethal impacts on human health. Our research aims to find Ni-resistant bacteria in the metal-contaminated soil that have a great potential for removing Ni from the environment. Attempts have been made to extract and characterize Ni-resistant bacteria from automobile and electroplating industry waste-contaminated soil using serial dilution, streak plating, and various morphological, biochemical, and genetic techniques. The maximum tolerable concentration of Ni and other heavy elements, such as cadmium, lead, and aluminium for the selected isolate, was investigated using the UV-Vis spectrophotometric method. Additionally, the bacterial strain's ability to remove Ni was assessed using an atomic absorption spectrophotometer.  The current research reveals a novel strain of Kluyvera cryocrescens that could withstand Ni, Cd, Pb, Al, and combinations of these heavy metals. The maximum tolerance concentration of K. cryocrescens M7 for Ni, Cd, Pb, and Al was found to be 150 ppm, 200 ppm, 1000 ppm, and 150 ppm, respectively. Additionally, it was also observed that the bacterial strain could remove Ni by 29.57%, 35.36%, 48.41%, 46.91%, and 44.88% after 12, 24, 48, 72, and 96 hours, respectively. The strain has also exhibited resistance to vancomycin, ampicillin, carbenicillin, and streptomycin. This research discovered a novel bacterial strain, K. cryocrescens M7 that may be beneficial for removing heavy metals, particularly Ni, from metal-contaminated soil.
    Keywords: Antibiotic resistance, bioremediation, Kluyvera cryocrescens, Metal removal, Nickel resistance}
  • MD Sabbir Hossain, Protima Sarker, Md. Rahaman, Fee Faysal Ahmed, Shaibur Molla Rahman, Md Khabir Uddin *
    Textile effluents are highly colored for synthetic dyes, cause significant water pollution due to high pH, TDS, EC, BOD, and COD content, and are harmful to aquatic species. Among different treatment processes, biological treatment process is considered as a promising approach. In this investigation, a mixed aerobic bacterial consortium was used for the treatment of wastewater. In addition, the fenton process with a normal sand filter was used for treatment and compared with the biological method. The mean values of BOD, COD, TDS, EC, DO, and pH in the raw wastewater indicated that the effluent was highly contaminated according to Bangladesh standard (ECR, 1997). Both the biological treatment process and fenton process separately showed promising removal of pollution load. The aerobic mixed bacterial consortium reduced TDS (66.67%), EC (60%), BOD (91.67%), and COD (85.45%) and fenton process reduced TDS (74.71%), EC (55.11%), BOD (88.33%), and COD (83.63%) compared to the raw effluent bacterial consortium simultaneously degraded dyes and decolorized the wastewater from dark deep green to transparent. Color removal for the mixed aerobic bacterial process after 72 hours of aeration was 58.57% and for the fenton process with a normal sand filter was 80%. BOD and COD removal percentages for aerobic mixed bacterial consortium showed higher removal efficiency than the fenton process with a normal sand filter. Though 92 hours of aeration showed the maximum satisfactory result, aeration time could be reduced to 72 hours which also satisfied the Bangladeshi standard (ECR, 1997).
    Keywords: Textile effluents, bioremediation, advance oxidation process, coagulation-flocculation, microbes}
  • الهام ملک زاده*
    زمینه و هدف

    در دهه‏های اخیر، آلودگی محیط‏زیست، به‏ویژه خاک به فلزهای سمی در سطح جهانی افزایش چشمگیری داشته است. ورود فلزهای سمی به خاک از منابع مختلف، تهدیدی همیشگی و جدی برای سلامت گیاهان، جانوران و جوامع انسانی است. زیست‏پالایی با به‏کارگیری میکروارگانیسم‏های مفید خاکزی باعث افزایش راندمان پالایش مناطق آلوده به فلز می‏گردد و جایگزین مناسبی برای روش‏های پالایش فیزیکوشیمیایی شناخته‏شده می‏باشد.

    روش بررسی

    قارچ‏های آربوسکولار مایکوریزا (AM) در اکوسیستم‏های مختلف دنیا از جمله در خاک‏های آلوده به فلزهای سمی حضور دارند. این قارچ‏ها توسط مکانیسم‏های مختلفی فلزهای سمی را در اندام‏های قارچی درون و برون ریشه‏ای غیرپویا کرده و علاوه بر کاهش اثر سمی فلزها بر گیاه میزبان، از ورود آن به زنجیره‏های غذایی بالاتر ممانعت به‏عمل می‏آورند. مقاله حاضر، به نقش گلومالین به‏عنوان مولکول مهم دیواره سلولی اسپور و هیف‏های قارچ AM در خاک‏های آلوده به فلزات سمی پرداخته است.

    یافته‏ ها

    نتایج نشان داد، گلومالین به‏عنوان محصول اختصاصی قارچ‏های AM، در نقش یک پروتیین شوک حرارتی و نیز ترکیب عمده و اصلی در دیواره هیف و اسپورها حضور دارد.

    نتیجه ‏گیری

    گلومالین از طریق کاهش خطر سمیت و قابلیت دسترسی زیستی فلزها برای گیاهان و سایر موجودات، در حفظ و ارتقای سلامت خاک نقش مهم و کلیدی ایفا می‏کند.

    کلید واژگان: سلامت خاک, زیست ‏پالایی, کمپلکس گلومالین-فلز, همزیستی مایکوریزی}
    Elham Malekzadeh *
    Aim and scope

    In the last few decades, contamination of the environment especially the soil by toxic metals has been increased extremely at worldwide. Entrance of toxic metals into the soil from various sources is a constant and serious threat to the health of plants, animals and human societies. Bioremediation by using of the beneficial soil microorganisms improves the remediation efficiency of the metal contaminated areas and is a suitable alternative method for substitution of current physico-chemical strategies.

    Methodology

    Arbuscular mycorrhizal (AM) fungi are found in virtually all ecosystems worldwide, including in soil contaminated with toxic metals. AM fungi sequestrate toxic metals at fungal intra- and extracellular structures by different mechanisms, so in addition to reduce their toxic effects on host plant prevent from their entrance in the food chain. This study has been addressed the role of glomalin as an important molecule of the cell wall of AM fungi spores and hyphae in soils contaminated by toxic metals.

    Finding

    The results showed that glomalin as a specific product of AM fungi, is present in the role of a heat shock protein as well a critical and main component of spores and hyphal cell wall.

    Conclusion

    Glomalin plays an essential and key role in maintaining and improving the soil health by reducing toxicity and availability of metals for symbiotic partner of AM fungi and other organisms.

    Keywords: Soil health, Bioremediation, Glomalin-metal complex, Mycorrhizal symbiosis}
  • هاجر آبیار*، زهره روستان
    آلودگی دریاها توسط فلزات سنگین یکی از عوارض اجتناب ناپذیر صنعتی شدن و توسعه ی جوامع بشری است. تحقیق حاضر به منظور جداسازی و شناسایی باکتری های مقاوم به مس و قابلیت آن ها در جذب زیستی مس انجام شد. نمونه های رسوب از 3 ایستگاه در خور موسی جمع آوری شدند و پس از کشت در محیط حاوی غلظت های مختلف مس، دو باکتری مقاوم به غلظت 100 میلی گرم در لیتر مس جداسازی شدند و توسط تست های بیوشیمیایی و آنالیز 16S rRNA شناسایی گردیدند. عملکرد این باکتری ها در جذب مس در محیط واجد این فلز بررسی شد. هر دو  باکتری گرم منفی، اکسیداز- کاتالاز مثبت و متعلق به جنس Ochrobactrum بودند و تنها در واکنش به تست های بیوشیمیایی PD (phenylalanine deaminase)، NaCl و اوره تفاوت معنی داری نشان دادند. سنجش جذب زیستی غلظت های متفاوت مس نشان داد که هر دو سویه بیش از نیمی از فلز مس را در مدت زمان 150 دقیقه در تمامی غلظت ها جذب کردند. عملکرد هر دو سویه در غلظت 50 میلی گرم در لیتر تقریبا مشابه بود. زیرا سویه های O. tritici strain AN4 و  O. anthropi strain YX0703 به ترتیب 73/5% و 72/6% مس را  حذف کردند. اما در غلظت 200 میلی گرم در لیتر، سویه O. anthropi strain YX0703 درصد جذب بالاتری را نشان داد و در عرض 150 دقیقه میزان مس را از 200 میلی گرم در لیتر به 2/06±72/08 میلی گرم در لیتر کاهش داد. نتایج مطالعه حاضر نشان داد که این دو باکتری دارای قابلیت بالایی در حذف فلز مس هستند و می توان از پتانسیل این گروه از باکتری ها در کاهش آلودگی فلزات سنگین در خلیج فارس استفاده نمود.
    کلید واژگان: سویه باکتری, تست های بیوشیمیایی, فلزات سنگین, کاهش زیستی}
    Hajar Abyar *, Zohreh Roostan
    The pollution of the marine ecosystems with heavy metals is one of the serious effect of industrialization and development of human society. For exploitation of marine sources and constant development, it’s necessary to invent modern methods for removal of heavy metal pollution. In present study to assess the possibility of removing copper, we aimed to investigate isolation and identification of resistant bacteria and determine their potential to adsorb copper through biosorption. Sediment samples were collected from 3 stations at Khor Mousa and cultured on nutrient agar medium containing different concentrations of copper. Two copper resistance (100 ppm) bacterial strains were isolated and identified through biochemical tests and 16S rRNA analysis. Also bacterial behaviors in adsorbtion of copper were studied. Both strains belonged to Ochrobactrum genus and were gram-negative, catalase and oxidase positive. Different characteristics of bacteria were observed in PD (phenylalanine deaminase), NaCl and Ureas tests. Biosorption measurements showed that strains could adsorb more than half of copper in various concentrations in 150 minutes. The bacterial behaviors were the same in 50 ppm concentration. %73.5 and %72.6 of copper were adsorbed by strains O. tritici strain AN4 and O. anthropi strain YX0703 respectively. However, O. anthropi strain YX0703 reduced the copper concentration from 200 ppm to 72.08 ppm in 150 minutes and showed the highest copper biosorption. The results of present investigation showed that both mentioned bacteria have high ability to remove copper and we can use the potential of this group of bacteria for removal of heavy metal pollution in Persian Gulf.
    Keywords: Bacterial Strains, Biochemical tests, heavy metals, Bioremediation}
  • حمید سودائی زاده*، فریبا حکم اللهی، امیرحسین ناطقی، منوچهر محمدی
    سابقه و هدف

    برخی از فرآیندهای صنعتی، معدن کاری و استفاده از کودهای شیمیایی و قارچ کش ها در کشاورزی امروزی، منجر به رها سازی فلزات سنگین و همچنین افزایش شوری خاک و آب ها می شوند به گونه ای که افزایش سطح جهانی آلودگی به فلزات روند نگران کننده ای به خود گرفته است. از طرفی وجود یک بیلیون هکتار زمین های شور در سراسر کره زمین که تنها با پالایش فیزیکی، شیمیایی و زیستی قابل استفاده در کشاورزی می باشند، تهدیدی برای امنیت غذایی محسوب می شوند. وجود معادن بسیار در دنیا وایران و در نتیجه فرسایش خاک و انتشار غبار از سدهای باطله موضوعی نگران کننده است. پتانسیل سیانوباکترها در ایجاد پوشش بیولوژیک خاک، جذب فلزات سنگین و شوری خاک ها نسبت به روش های فیزیک وشیمیایی رایج، از جنبه کاربردی و اقتصادی بودن در حال بررسی می باشد.

    مواد و روش ها

     در کارخانه طلای موته در نتیجه عملیات استخراج طلا به وسیله سیانوراسیون، روزانه پساب و رسوب خاک باطله به صورت گسترده ای حاصل می شود. در مهرماه 97 جمع آوری نمونه های خاک باطله از معدن طلا انجام شد. نمونه های خاک باطله معدن جهت شناسایی و رشد ریزجلبک ها، در محیط کشت اختصاصی جلبک ها کشت داده شد. قبل و بعد از قرارگیری 5 گرم خاک باطله معدن در معرض 5/0 گرم زیست توده سیانوباکتری ها، عناصر سدیم، پتاسیم و فلزات سنگین سرب و کادمیوم خاک باطله اندازه گیری شد و داده ها مورد آنالیز آماری قرار گرفت.

    نتایج و بحث

     پس از کشت خاک معدن، سیانوباکترهای Oscillatoria tenuis C.Agardh ex Gomont ،tenue Gomont Phormidium، Lyngbya aestuarii Liebman ex Gomont و جلبک سبز Scenedesmus obliquus (Turpin) Kützing شناسایی شدند. نتایج کشت سیانوباکتری های رشته ای روی خاک های باطله معدن طلا نشان می دهد که این ریزموجودات فتوسنتز کننده می توانند به راحتی روی خاک های باطله آلوده معدن رشد کنند و پوشش بیولوژیک سطحی قابل ملاحظه ای را ایجاد کنند. همچنین نتایج نشان داد که O. tenuis، عنصر سدیم را بطور معنی داری کاهش داده است و P. tenue بیشترین میزان جذب سرب را داشته است و احتمالا با خالص کردن این دو گونه و استفاده جهت رشد روی خاک باطله معدن طلای موته می توان معدن را از شوری و عناصر سنگین به مقدار زیادی پاک سازی و همچنین از فرسایش خاک و انتشار غبار از سدهای باطله جلوگیری نمود.

    نتیجه گیری

    با توجه به وجود تعداد فراوان معادن مختلف طلا در دنیا و ایران، استحصال این فلز گران بها به طور وسیعی با استفاده از سیانور صورت گرفته که بدلیل سمیت بالا دارای اثرات سوء زیست محیطی زیادی می باشد. انتشار غبار سیانور از سد باطله می تواند سلامتی موجودات زنده نزدیک به آن از جمله کارگران معدن، گیاهان و حتی ریزجلبک های خاک زی را تحت تاثیر قرار دهد. با زیست پالایی خاک معادن می توان فرآیند احیای زیستی را با سرعت بیشتری انجام داد. نتایج کار محققان معادن طلای آفریقای جنوبی نشان می دهد که سیانوباکتری ها، پوشش بیولوژیکی سطحی قابل ملاحظه ای را روی خاک باطله ایجاد می کنند که با نتایج حاصل از این تحقیق مبنی بر رشد Phormidium سیانوباکتر مقاوم معدن طلای موته روی باطله ها و ایجاد پوشش بیولوژیک گسترده روی خاک مطابقت دارد.

    کلید واژگان: Phormidium, فلزات سنگین, زیست پالایی, معدن طلای موته}
    Hamid Sodaeizade *, Fariba Hokmollahi, AmirHossein Nateghi, Manouchehr Mohammadi
    Introduction

    Natural and human activities lead to soil degradation and soil salinization. In the last two centuries, world metal pollution level has increased extremely. Presence of some heavy metals in aquatic ecosystems is a constant threat to the health of human societies. The decrease of farmlands threatens food security. There are approximately 1 billion ha salt-affected soils all over of world, which can be made available resources after chemical, physical and biological remediation. Existence of many mines in the world and also in Iran and as a result, soil erosion and dust release from waste dams is matter of concern. Bioremediation using biological agents to detoxify and degradation of environmental pollutants- provides a suitable alternative method for substitution of current heavy metals removal strategies.

    Material and methods

    Daily as a result of extraction operations by cyanidation in Mouteh gold mining complex, waste water and waste soil sediment widely achieved. In this study soil samples were collected from Mouteh Goldmine tailing damp soil in September 2018. The collected soil samples were cultured in BG11 medium and incubated for microalgae identification and biomass production. Also 5 gr soil inoculated with 0.5 gr biomass and the physico-chemical characteristics of the soil including pH, Na+ and K+, Pb and Cd, before and after the inoculation of soil with Cyanobacteria were determined.

    Results and discussion

    in this study, cyanobacteria Phormidium tenue Gomont, Osillatoria tenuis C.Agardh ex Gomont, Lyngbya aestuarii Liebman ex Gomont and green Algae, Scenedesmus obliquus (Turpin) Kützing were identified. Culture results of filamentous cyanobacteria on goldmine tailing damp soil showed that cyanobacteria can grow easily and produced a surface crust significantly. The results of the Physical analysis of the soil samples showed that O. tenuis decreased Na as a salinity element, and P. tenue absorbed high amounts of Pb as a heavy metal pollution element. Probably by using Mouteh Goldmine soil cyanobacteria, Osillatoria tenuis and Phormidium tenue for mine tailingdamp soil bioremediation, could play mine recovery from salinity and heavy metals and also preventing soil erosion and dust release from waste dams.

    Conclusion

    Generally, it can be concluded thatdue to the existence of many goldmines in the word and Iran, daily as a result of extraction operations by cyanidation and high toxicity of cyanide, environmental adverse effects are observed. Dust release from waste dams can be affected health of living organisms like mining workers, plants and even soil microalgae which are close to the mine. By soil goldmine bioremediation can be processed biological resuscitation faster. The research results of African goldmine researchers on soil samples showed that cyanobacteria produced a surface crust significantly, that with the results of this research based on the growth of Phormidium tenue in the mine and producing a surface crust is consistent.

    Keywords: Phormidium, Heavy Metal, Bioremediation, Mouteh Goldmine}
  • فرهود نوائی، عباس هاشمی زاده*

    آلاینده‌های هیدروکربن‌های نفتی، از لحاظ تجزیه و مقابله جزء ترکیب‌های سخت بوده و به عنوان آلاینده‌های آلی پایدار و مهم طبقه بندی می‌شوند.‌‌ این آلاینده‌ها اثرات نامطلوبی بر سلامت انسان و محیط پیرامونش دارند و مقابله با آلودگی محیط زیست ناشی از آن‌ها مسئله مهمی برای جهان و جوامع بشری است. هرچند پاکسازی این آلاینده‌ها از محیط زیست یک مشکل اساسی است، اما تجزیه بیولوژیکی که از فعالیت بیولوژیکی طبیعی و تجزیه میکروبی استفاده می‌کند‌‌، یک روش سازگار با محیط زیست و اقتصادی برای کنترل این نوع آلودگی‌ها است و در پاک‌سازی محیط‌های آلوده با هیدروکربن‌های نفتی به یک روش اصلی تبدیل شده است. پژوهش حاضر یک بررسی جامع، به‌هنگام و کارآمد درباره تصفیه زیستی آلاینده‌های هیدروکربنی نفت خام با در نظر گرفتن دگرگونی و تغییرات هیدروکربن‌ها توسط میکروارگانیسم‌ها با تمرکز ویژه بر بینش‌های جدید به وجود آمده در چند سال اخیر را ارایه می‌دهد‌‌. همچنین متابولیسم هیدروکربن‌ها در میکروارگانیسم‌ها با مرور تحقیقات ارایه شده در چند سال اخیر تشریح شده است. نتایج مطالعه حاضر به خوبی نشان می‌دهد آلاینده‌های هیدروکربن‌های نفتی با استفاده از برخی میکروارگانیسم‌ها مانند اولیوفیلیک، تا حد قابل قبولی زیست‌تجزیه‌پذیر بوده و حذف آن‌ها با این روش مقرون به صرفه و اقتصادی است‌‌، همچنین تجزیه بیولوژیکی میکروبی آلاینده‌های هیدروکربن نفتی از فعالیت‌های کاتالیزوری آنزیمی میکروارگانیسم‌ها استفاده می‌کند و میزان تخریب آلاینده‌ها را تا چندین برابر روش‌های سنتی افزایش دهد‌‌.

    کلید واژگان: تخریب میکروبی, آلاینده های هیدروکربنی نفتی, تجزیه بیولوژیکی, پالایش زیستی}
    Farhood Navaie, Abbas Hashemizadeh*

    Petroleum hydrocarbon pollutants are one of the hardest compounds in terms of decomposition and control and classified as stable and important organic pollutants that have adverse effects on human health and the environment and combating environmental pollution caused by them is an important issue for the world and human societies. Although the removal of these pollutants from the environment is a major problem, biodegradation (which uses natural microbial biodegradation activity) is an ecofriendly and economical approach to control these types of contaminants and has become a pivotal method of cleaning up environments contaminated with petroleum hydrocarbons. The present study provides a comprehensive, uptodate and efficient review of the bioremediation of petroleum hydrocarbon pollutants, taking into account the hydrocarbon alterations in microorganisms with a particular focus on the new insights gained in recent years. Also, the metabolism of hydrocarbons in microorganisms has been described by reviewing research presented in recent years. The results of studies show well that petroleum hydrocarbon pollutants are biodegradable using some microorganisms such as oleophilic and their removal by this method is cost-effective and economical. Microbial biodegradation of petroleum hydrocarbon contaminants uses the enzymatic catalytic activities of microorganisms to increase the degradation of contaminants several times more than traditional methods

    Keywords: Microbial degradation, Petroleum hydrocarbon pollutants, Biodegradation, Bioremediation}
  • اکبر قویدل، سمیه ناجی راد*، حسینعلی علیخانی
    زمینه و هدف

    پالایش زیستی روشی است که در آن از توانایی ریزجانداران جهت افزایش میزان و سرعت تجزیه آلاینده ها و درنتیجه کاهش آلودگی های محیط زیست استفاده می گردد. رطوبت و دما از عوامل محیطی اصلی تاثیرگذار بر رشد و فعالیت ریزجانداران و بالطبع بر کارآیی تجزیه زیستی آلاینده های آلی می باشند.

    روش بررسی

    به منظور بررسی تاثیر این دو فاکتور یک آزمایش فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار انجام شد. فاکتورهای مورد بررسی شامل؛ رطوبت در سه سطح (30%، 55% و 80% ظرفیت مزرعه)، دما در سه سطح (25، 30 و 35 درجه سانتی گراد) و تلقیح با باکتری در دو سطح (تلقیح شده با باکتری سودوموناس پوتیدا و بدون تلقیح با باکتری) بودند.

    یافته ها

    نتایج حاصل از تحقیق نشان داد که بیش ترین میزان تجزیه زیستی نفت خام در تیمار دارای شرایط رطوبت 55% ظرفیت مزرعه، دمای 30 درجه سانتی گراد و تلقیح با سودوموناس پوتیدا مشاهده شد که معادل 8/92% و کم ترین تجزیه در تیمار رطوبت 30% ظرفیت مزرعه، دمای 30 درجه سانتی گراد و بدون تلقیح با باکتری معادل 3/42% بود.

    بحث و نتیجه گیری

    این نتایج نشان می دهد که بهینه سازی عوامل محیطی در زیست پالایی می تواند سبب افزایش کارآیی پالایش تا 5/50 % شود.

    کلید واژگان: آلودگی نفتی, زیست پالایی, شرایط محیطی, سودوموناس پوتیدا}
    Akbar Ghavidel, Sumayyah Naji Rad *, HosseinAli Alikhani
    Background and Objective

    Bioremediation is an approach that exploits the ability of microorganisms to increase the rate and extent of degradation of pollutants and thereby removing pollutants from the environment. The moisture content and temperature are of the main environmental factors affecting growth and activity of microorganisms and accordingly affecting the efficiency of organic pollutant biodegradation.

    Method

    To study the effect of these two factors a factorial experiment was carried out as completely randomized design with three replications. The factors were moisture in three levels (30%, 55% and 80% of Field Capacity), temperature in three levels (25, 30 and 35 degrees of Celsius) and inoculation with bacteria in two levels (with and without inoculation by Pseudomonas putida) which were triplicated.

    Findings

    The results showed that highest biodegradation rate was observed in the treatment with the moisture content of 55% F.C, temperature of 30 degrees of Celsius and inoculation with Pseudomonas putida which was 92.8% and the lowest biodegradation rate was observed in the treatment with the moisture of 30% F.C, temperature of 30 degrees of Celsius and without inoculation which was 42.3%.

    Discussion and Conclusion

    These results shows that the optimization of the environmental conditions in bioremediation process may lead to 50.5% increase in the efficiency of removal.

    Keywords: Crude Oil Contamination, Bioremediation, Environmental Conditions, Pseudomonas Putida}
  • T. Salehi, D. Yousefi Kebria *
    The present study focused on the synergistic effects bioremediation of phenol in aqueous solution using combination of anaerobic mixed culture and Granular Activated Carbon (GAC) as a biological GAC (BGAC). Meanwhile, the effect of contact time and various phenol concentrations on adsorption and biosorption process investigated. The phenol concentration was analyzed using UV/Vis spectrophotometer. The morphology and structure of two adsorbents (GAC and BGAC) were characterized by FESEM and BET specific surface area analysis. The batch experiments using mixed bacterial culture, isolated from wood and paper factory wastewater, were adapted to high concentrations of phenol and employed in order to evaluate the tolerance and biosorption capability of microorganisms for phenol biodegradation. The synergetic effect of phenol removal using combination of GAC with an anaerobic biofilm indicated that the removal efficiency for concentration of 700, 800, and 1000 mg/l at initial stages increased to 4, 10, and 12%, respectively and while by increment of the retention time did not shown significant impact on the removal efficiency. These results conducted both desorption of adsorbates due to biotransformation in the aqueous solution and direct assimilation of adsorbates on GAC by the microorganism’s biofilm. The adsorption data were fitted with pseudo-first-order and pseudo-second-order models and it was found that the pseudo-second-order model explains the adsorption kinetics more efficiently. The compatibility of the Freundlich and Langmuir adsorption models to equilibrium data were investigated. In fact, the Langmuir isotherm was found to be the best fitting isotherm.
    Keywords: Anaerobic culture, Bioremediation, Equilibrium Isotherms, Granular Activated Carbon, Kinetic Models, Phenol}
  • A. A. Pourbabaei *, E. Khoshhal Nakhjiri, E. Torabi, M. Farahbakhsh
    Butachlor (BUT) is a chloroacetanilide herbicide widely applied to rice paddies to control annual grass and broad-leaf weeds. A BUT-degrading bacterial strain (PK) was isolated from paddy soils. Biochemical and 16S rRNA sequencing characteristics confirmed the strain as Pseudomonas aeruginosa (99% resemblance). The isolate dissipated BUT (100 μg/mL) in an M9 liquid medium with a rate of 0.5 ± 0.03 day-1 and DT50 and DT90 of 1.38 ± 0.10 days and 4.58 ± 0.32 days, respectively. Soil dissipation of BUT was investigated under flooded conditions. In sterile soils, the isolate increased the dissipation of BUT (200 μg/g) (DT50 = 12.38 ± 1.83 days, DT90 = 41.12 ± 6.09 days, k = 0.06 ± 0.01 day-1) compared to sterile non-inoculated samples (DT50 = 26.87 ± 2.82 days, DT90 = 89.25 ± 9.36 days, k = 0.03 ± 0.00 day-1). In non-inoculated non-sterile soil experiments, the dissipation of BUT was faster (DT50 = 15.17 ± 2.11 days, DT90 = 50.38 ± 7.02 days, k = 0.05 ± 0.00 day-1) compared to non-inoculated sterile ones, and inoculating the isolate accelerated the removal of BUT in non-sterile soils significantly (DT50 = 8.03 ± 1.20 days, DT90 = 26.68 ± 3.97 days, k = 0.09 ± 0.01 day-1). BUT inhibited soil respiration (SR) initially for 5 days, followed by an increase until day 20. The increase in SR was more pronounced in the co-presence of BUT and the isolate. The results of this research suggest P. aeruginosa PK as a suitable candidate for BUT bioremediation.
    Keywords: Bacterial isolate, bioremediation, Pseudomonas aeruginosa, Paddy, Soil respiration}
  • N. I. Ihimekpen, E. Atikpo *, N. Kayode Ojo, A. Michael

    The abilities of three indigenous bacteria for bioremediation of cadmium contaminated soils collected from Agbabu Farm Settlement close to mining sites in Ondo state, Nigeria was studied to provide helpful information for soils remediation and soils health management in this sub-region for Millennium Development Goals accomplishment. Bacillus subtilis, Proteus mirabilis, and Escherichia coli isolated from the soils were inoculated into different soil samples conditioned with optimized factors determined from the first phase experiments. The conditioned samples were experimented for residual cadmium concentration with time in days using Atomic Absorption Spectrophotometer. The soil cadmium attenuation from the initial concentration of 70.21 mg/kg to below the maximum allowable of 3 mg/kg was hard for the organisms. Bacillus subtilis performed correction at time 35 days with an efficiency of 96.10 % and residual concentration of 2.74 mg/kg. Proteus mirabilis and Escherichia coli with respective, high efficiencies of 85.05% and 79.35% failed. The removal rate capacities were -0.131d-1 for B. subtilis; -0.111d-1 for P. mirabilis; -0.105d-1 for E. coli. Four kinetic models fitted described the experimental data well. The models assessment revealed the removals to be transport controlled as diffusion process was the rate-controlling step.

    Keywords: Bioremediation, Biosorption, Cadmium Removal, Contaminated Soil, Diffusion, heavy metals}
نکته
  • نتایج بر اساس تاریخ انتشار مرتب شده‌اند.
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