جستجوی مقالات مرتبط با کلیدواژه "fusarium head blight" در نشریات گروه "کشاورزی"

Fusarium head blight disease is one of the most important and destructive diseases of wheat in hot and humid climates, which, in addition to reducing yield, causes the accumulation of mycotoxins in the grain, which is dangerous for humans and animals. Passive resistance against Fusarium includes many morphological and phenological traits such as plant height, flowering time, heading time, number of spikes and spike density, which help to escape the disease or create a kind of resistance to Fusarium head rot disease. The purpose of this study was to compare the studied genotypes with respect to Fusarium head blight disease and to estimate the simple and canonical correlation between morphological traits and disease resistance traits.

In order to evaluate 92 improved and native genotypes of Iranian bread wheat against Fusarium head blight in two crop years (2019-2022), experiments were conducted in the form of a randomized complete block design in the farm of Gorgan University of Agricultural Sciences and Natural Resources. Morphological traits measured included plant height (PH), spike length (SL), and number of seeds per spike (TSS), and agronomic traits measured included heading time (DTH) and flowering time (DTF). Disease traits were: disease incidence (INC), disease severity (SEV), Fusarium damaged kernels (FDK), FHBdx index and ISK.

For all disease-related traits and morphophenological traits, a significant difference (p<0.001) was observed between genotypes. According to the result of correlation analysis, there was a positive and significant correlation (p<0.001) between DTH, DTF and PH traits with traits related to Fusarium head blight of wheat. The analysis of canonical correlation between the variables of disease-related traits and morphophenological traits showed a significant canonical correlation (0.687), which explains 81% of the total variance. According to the results of cluster analysis based on morphophenological traits and traits related to FHB disease, the investigated genotypes were classified into five separate group, the genotypes of the first group had a lower average among the other groups and the average of all genotypes in terms of all traits related to FHB disease and at the same time, compared to other groups, they were earlier.

Rizhav, Chamran, Darya, Arta, Neishabur and Gonbad cultivars showed the highest resistance to Fusarium disease in the study and can still be used as parental cultivars to improve new wheat cultivars resistant to Fusarium Head Blight disease. Also, among the landrace, three genotypes, Larg Karaj, Parand Saveh, and Malayer, showed acceptable resistance, which indicates that some landrace cultivars are also relatively resistant to Fusarium head blight disease, and it may be possible if they have complementary gene loci of specific genes. Resistance to this disease can probably be used for breeding purposes and programs for resistance to Fusarium disease, which needs further investigation in future studies.

Using resistant cultivars is an effective method in management Zymoseptoria tritici Blotch and Fusarium Head Blight of wheat. This study was conducted to identify new sources of resistance to these diseases among a large number of Iranian cultivars and new wheat genotypes. The genotypes were cultivated in an augment design in the research farm of Aliabad Katoul Agricultural School. Artificial contamination of the genotypes were done using spreading infected leaves and inoculating with a pathogenic fungal spore suspension. Response of the genotypes to disease-causing were noted. Cluster analysis categorized genotypes into seven groups. Third and fourth groups were allocated as resistant and sensitive, respectively. Genotypes No 3 (Hirmand), 6, 10, 12, 13 (Golestan), 38 (Aftab), 40 (ghabous), 44 (Pishtaz), 51, 52 (shoosh), 59 (Pastor), 64, 68, 73 (Sepahan), 87, 92, 95, 97, 99, 110, 116, 127 (Line 7) and local check cultivar (Ehsan) were classified at group three (resistant). This group had the lowest area under the disease progress curve and showed high resistance to disease. Resistant genotypes identified in this study can be used in breeding programs for pyramiding resistance genes or using backcrossing method.

Fusarium head blight disease caused by Fusarium graminearum is one of the most important and destructive diseases of wheat in warm and humid zone. The importance of this disease is to reduce the yield and accumulation of mycotoxin in the harvested grains from infected heads, which are dangerous to humans and animals. Production of resistant varieties with using genetic resistance sources is the most effective method of sustainable control of this disease. To study the inheritance of resistance-controlling genes in cv. Morvarid, the resistant cv. Morvarid, was done cross with susceptible cv. Falat, and generations of F1, F2, BC1 and BC2 were produced. Resistance components include the area under the disease progress curve (AUDPC), the relative area under the disease progress curve (rAUDPC) and the final severity infection (FSI), was recorded. Parents and generations were planted in a randomized complete block design with three replications. Parents and generations were inoculated at the adult plant stage by a mixture of Fusarium head blight isolats at the Gorgan Research Station. Analysis of variance showed significant differences among generations for measured traits. The average degree of dominance was approximately equal to unit (one) for all traits, indicating dominance genetic control. Epistasis interaction effects of additive × dominance, dominance × dominance were significant for the all traits. Broad-sense (0.45- 0.56) and narrow-sense (0.37-0.48) heritability was estimated as moderate, indicating the important role of additive effects and dominance in genetic control of these traits. The number of segregating genes for measured traits 1 to 7 genes was estimated. Since Morvarid Cv. is currently the most commercially resistant cultivar to Fusarium head blight, due to the additive effects of resistance genes in controlling these traits, selection can be applied to improve these traits in the early generations of this cultivar.

Fusarium Head Blight (FHB) is one of the most important diseases of wheat causing considerable yield losse annually. Commonly management practices of the disease are not effective, so adopting new strategies to control are seriously needed. For this purpose, in the present study, efficiency of commercial and local Trichoderma strains was evaluated in the control of Fusarium Head Blight on wheat. For isolation of the pathogen and antagonists, samples were taken from infected wheat spicke and wheat rhizosphere, respectively. Identification of fungal isolates (Trichoderma and Fusarium isolates) was performed based on combination of morphological characteristics and molecular data. Accordingly, the identity of the 10 isolates of the pathogen and 13 isolates of the antagonists was determined as Fusarium graminearum and Trichoderma harzianum, respectively. In lab, after a preliminiary screening, a superior Trichoderma isolate from wheat rhizosphere (T. harzianum Tr5), one commercial product (T. harzianum T22) and T. longibrachiatum N(prepared from culture collection of Tabriz University,) were used to study their antagonistic activity against F. graminearum under laboratory and greenhouse conditions. Antagonistic strains were furhter assessed in the production of chitinase and protease enzymes. The results of laboratory tests showed that all fungal antagonist strains significantly inhibited the growth of the pathogen compared to control. In dual culture assay, the highest inhibitory percent was detected in the interaction of F. graminearum with T. harzianum Tr5and T. longibrachiatum N. Furthermore, only T. harzianum Tr5 was able to show mycoparasitism mechanism directly. The volatile compounds of the antagonist strains showed the lowest inhibitory against pathogen, without any significant difference between them. In non-volatile compounds assay, the highest inhibitory percentage was detected for T. longibrachiatum N and the lowest inhibitory percentage was detected for T. harzianum Tr5 and T. harzianum T22, respectively. All antagonists were able to produce chitinase and protease enzymes, with more capability for local isolates. Finally, the results of the greenhouse assay were positively in consistent with the laboratory tests. The results of greenhouse assay showed that the antagonist isolates significantly inhibited the adverse effects of the pathogen on weight loss of wheat grain.

Fusarium Head blight (FHB), caused by Fusarium graminearum known as a destructive disease of wheat has a significant impact not only on global wheat production, but also on food safety because of grain contamination with mycotoxins during infection, a serious threat to the human and animals’ health. To achieve to non-chemical control of FHB, the study of molecular mechanism of resistance to FHB in wheat varieties is important. In this research, in order to investigate the effect of FHB on spike proteome pattern, six bread and durum wheat cultivars were inoculated in a greenhouse with F. graminearum. In addition to measuring the morphological traits, 14 days after inoculation, changes in their proteome pattern were investigated. Two-dimensional electrophoresis technique was used to study the response of wheat spike to Fusarium. Protein extraction was performed by TCA-acetone method and two-dimensional electrophoresis was performed in the first dimension by strip gel method and in the second dimension was performed by using sodium dodecyl sulfate polyacrylamide gel. The results of gel analyses showed that out of 57 repeatedly found protein spots, 13 protein spots had a significant difference between treatments, among which 11 protein spots, showed increased expression and two protein spots showed reduced expression. Proteins with changes in expression based on biological function were categorized in different groups that proteins involved in the metabolic pathways which involved in infected plants (Hypothetical protein and PR proteins) had the highest contribution to proteins with significant changes. The results of this study can be used in wheat breeding programs.

In this study, 224 wheat genotypes which had good agronomic characteristics were selected between 1700 collection of Cereal Research Department, Seed and Plant Improvement Institute, to evaluating against diseases FHB, leaf rust and powdery mildew resistance in field condition in Golestan province. After land preparation, seed of each genotype were planted on a line with length 1.5 meters below mist irrigation systems. Spikes at 50% flowering (stage 65 of the Zadoks) were inoculated with spore suspension of fungi isolates of Fusarium head blight (Fusarium graminearum, F. culmorum) within three days of one another in two stages. To evaluate genotypes resistant to leaf rust, in stem elongation (stage 35 of the Zadoks), were sprayed with a mixture of spores of the pathogen. Genotypes were evaluated to powdery mildew with naturally infected area. Notes the reaction of genotypes diseases mentioned above, the standard method of assessment for all diseases. The results showed that, 60 percent of genotype in disease incidence of Fusarium head blight were moderately susceptible and 30% were susceptible reaction and the rest were moderately resistant and resistant, but in disease severity FHB, 30% of moderately susceptible reaction, 37% susceptible and the rest were placed in resistant and moderately resistant groups. In the disease index FHB of genotypes showed that 20 percent moderately susceptible, 22% susceptible, 34% moderately resistant and 24 percent showed good relative strength. Results evaluated genotypes reaction to leaf rust races in the region showed that 43% resistant, 47% susceptible and others 10 percent were moderately resistant or moderately susceptible. In this study, 17% of genotypes were resistant, 25% moderately resistant, 27% moderately susceptible and 31% were quite susceptible. After analysis of the data, 20 genotypes were favorable partial resistance to any of the diseases studied, were introduced as sources of resistance and molecular genetics of resistance to genetic unit Cereal Research Department, Seed and Plant Improvement Institute.

An improved method of conidia production is described and compared to a conventional method growing Fusarium species on LSPDA medium under near-ultraviolet (NUV) light for 21 days. In the improved procedure, Fusarium spp. were grown in PDB for three days and subsequently spread as aliquots onto LSPDA, SNPMA and WA media. To remove excess water, the cultures were dried in a laminar flow cabinet for 20 to 30 min. Air-dried plates were incubated under NUV light at 25 ºC for 3 days. To compare aggressiveness of conidia produced by either method, wheat ears were inoculated at the mid-flowering. Disease incidence (% infected kernels) and Fusarium head blight severity as well as fungal biomass produced in the infected kernels were determined. The improved method effectively triggered sporulation of Fusarium spp. and gave high yields of conidia per unit of area within two days of incubation on solid media which is significantly higher compared to spore quantities produced with the conventional method. The average number of conidia produced by Fusarium spp. using the improved and conventional methods ranged from 56 to 156 and 0.8 to 38.6×106 (per plate), respectively. Spore production quantity was highly variable with the conventional method using different Fusarium species. However, morphology of conidia was similar in both assays. The aggressiveness of Fusarium inocula produced by both methods was not significantly different with respect to the ability to cause FHB and to colonize wheat kernels (P≤0.05). Microscopy examination showed the high conidiation rate from phialides on hypha.

Fusarium head blight (FHB) disease caused by Fusarium graminearum is one of the most destructive diseases of wheat that in addition to the loss of product quality and quantity is able to produce the dangerous carcinogenic toxins in human and animals. In this study, the effects of different fertilizer treatments (Vermicompost, Chemical fertilizer and Mycorrhiza) on performance components and expression of several genes involved in disease resistance pathway was investigated. For this reason, Falat wheat cultivar was used in a completely randomized design with four repetitions in greenhouse conditions. The analysis of variance showed that the effects of different fertilizer treatments on 100-seed weight, plant height, weight per plant, seed performance, biological performance and the harvest index were significant at 1% probability level. The analysis of gene expression data showed that beta Glucanase and Oxalate Oxidase genes had a significant increase in all treatments but the highest increase was observed in vermicompost and chemical fertilizers mixture. The results indicated that the mix application of biologic and non-biologic fertilizers could be induce systemic acquire resistance in infected plants.

Fusarium head blight (FHB) is economically one of the most important fungal diseases of wheat in the world. The aim of this research was to determine the antifungal metabolites of native Bacillus isolates. Bacillus strains isolated from soil of wheat and barley fields (Rural areas of southern Tehran, autumn 2012), and exhibited in vitro antagonistic activity against some Fusarium species isolated from infected wheat seeds (Wheat fields of Parsabad at Moghan, Ardebil Summer 2012). An attempt was made to partially purify and characterize the diffusible antifungal metabolite/s produced by the selected Bacillus strain in Nutrient broth medium. High Performance Liquid Chromatography (HPLC) of partially purified extract of the strain showed the presence of lipopeptide antibiotic iturin as a major peak that was comparable to that of standard iturin A (11.80 min) from Sigma–Aldrich. The structure was further confirmed by Fourier Transform-Infrared Spectrum (FTIR) and Liquid Chromatographic Mass Spectrometric (LCMS) analysis as iturin A. LCMS analysis also showed the presence of fengycin with iturin A. The genome of the selected isolate of Bacillus had shown 99/9 percent similarity by B. aryabhattai and the genome of the selected isolates of Fusarium had shown 99 percent similarity by F. graminearum. According to the results of this experiment, the antifungal effects of native Bacillus strains and also their effects in the biological control of wheat FHB disease were confirmed.

The majority of viruses infected fungi which have been reported contain double-strand RNA (dsRNA) genomes. Mycoviruses are introducing as agent of the biological control of fungi according to hypersensitive properties. In this research, eight isolates belong to Fusariumgenus include ofF. graminearum, F. culmorum, F. tricinctum, F. proliferatum and F. incarnatum survived to dsRNA infection. Due to using of hazardous material such as phenol and complicated procedure in different dsRNA extraction protocols in mentioned research phenol and chlorophorm was removed , using less tissue and dsRNA extraction time was reduced compared to other methods. Result showed that four isolates infected by dsRNA also, mentioned method has sufficient efficiency and convenient for dsRNA extraction from fungi tissue. Also, dsRNA was reported fromF. incarnatum (F. semitectum) for first time.

Fusarium culmorum which is considered as the causal agent of Fusarium head blight، can produce deoxynivalenol (DON)، nivalenol (NIV) and their acetylated derivatives. This study has been assessed in order to detect the Tri13 gene using primer pairs Tri13DONR/Tri13F and Tri13R/Tri13NIVF، to investigate the potential of F. culmorum isolates in the production of DON and NIV in West Azarbayjan province، Iran. In this study، 98 F. culmorum isolates were obtained from Urmia، Khoy، Miandoab، Maku، Tekab and Mahabad regions. For this purpose، F. culmorum isolates were identified based on the morphological characteristics as well as specific primer pairs، C51F/C51R. Production of DON and NIV were detected in these isolates according to Tri13 gene. Results showed that 60. 2 percent of isolates have the potential to produce DON and 39. 8 percent of isolates have ability to produce NIV.

To evaluate resistance of wheat advanced and elite lines to fusarium head blight (Fusarium graminearum), 61 genotypes obtinedfrom Cereal Research Department of Seed and Plant Improvement Institute were grown at agricultural research field of Agricultural and Natural Resources Reserch Center of Gorgan in 2012 using a randomized complete block design with three replications. Inoculums of the pathogen was increased on wheat straw in laboratory and a mixture suspension of spores of  five isolates were sprayed on plants at two growth stages of heading and anthesis.After development of disease in field, disease incidence, disease severity and disease index were assessed for each line. Based on the results, none of the lines were immune or resistant, but lines ARWYT-N-90-2, ERWYT-N-90-20 and ERWYT-N-90-4 with lower disease index exhibited an acceptable level of resistance.

Acetyl transferases are enzymes responsible for enzymatic transfer of an acetyl group to suitable receptor molecule by using acetyl CoA as donor. Acetyltransferase reaction is involved in biosynthesis pathway of some important secondary metabolites such as antibiotics، as well as in their detoxification. Trichothecenes are significant secondary metabolites produced by the plant fungal phatogens Fusarium ssp. Such as F. sporotrichioides and F. graminearums. These fungi possess specific genes in their genomes encodeinge acetyl transferase enzymes are affecting trichothecene. In this study، the gene encoding acetyltransferase from the fungus F. sporotrichioides، TRI 101، was cloned and transferred into tobacco plant as model plants and the effect of the enzyme on the detoxification of deoxynivalenol (DON)، a well known trichthecene، was investigated. In addition it was shown that، in comparison whit the roots of wild type plants، transgenic roots grew normally in the deoxynivalenol-contained medium.

Fusarium head blight (FHB) is a disease that causes major economic losses in wheat and other cereal crops production worldwide. Contamination of food with the trichothecene mycotoxin deoxynivalenol (DON) produced by Fusarium graminearum is a major health concern for humans and animals because trichothecenes are potent cytotoxins of eukaryotic cells. Trichothecene mycotoxins inhibit translation by targeting ribosomal protein L3 at the peptidyl transferase center. In this study, we modified a Tomato (Lycopersicon esculentum) cDNA encoding the ribosomal protein RPL3 so that amino acid residue 258 is changed from tryptophan to cysteine and amino acid residue 259 is change from histidine to tyrosine. All version of the tomato RPL3 were introduced to DON-sensitive pdr5 and ayt1 mutant strain Saccharomyces cerevisiae. When transgenic yeast were compared for growth in the presence of DON, a difference in growth rate and survival was observed among those yeasts expressing the modified versions of the tomato RPL3 genes, compared to those expressing the wild-type yeast RPL3 gene. These results can create a new field in developing FHB resistance varieties of wheat through genetic manipulation.

Fusarium Head Blight (FHB)، which is caused commonly by Fusarium graminearum، has ability to result significant reduce in yield and also cause indirect losses due to the accumulation of potent mycotoxins (trichothecenes) in harvested grain as secondary metabolites; which are hazardous for human and animal. Trichothecene mycotoxins (such as DON) are potent protein synthesis inhibitors for eukaryotic organisms. Trans expression of AYT1 gene from S. cerevisiae capable of trichothecene 3-O-acetylation and converts DON to a less toxic acetylated form. One of the detoxification and resistance to mycotoxins is acetylation. The main goal of this study was to evaluate transgenic tobacco model plants to deoxynivalenol (DON). In order to detect expression of the AYT1 transgene، we added cMyc tag via PCR-Tagging method and introduced it into the model tobacco plants through Agrobacterium-mediated transformation in an attempt to detoxify DON. After confirmation of integrations of AYT1-cMyc into the tobacco genome by molecular analyses، Immuno-blotting and serological protein studies and trichothecene acetyl transferase activity analyses confirmed the expression of AYT1 and tolerance to 10 ppm concentration of DON in transgenic lines was observed.

Deoxynivalenol (DON) is a major mycotoxin produced by the fungus Fusarium graminearum during infection and is known to be harmful to both animal and human health. DON is believed to act as a virulence factor in fungal pathogenesis by inhibiting eukaryotic protein synthesis. The putative site of action of DON is 60s ribosomal protein L3 (RPL3). Previously, single amino acid changes in RPL3 conferring DON resistance have been described in yeast. Sequence characterization of six wheat genes encoding RPL3 has shown that there is no amino acid alteration between resistant and susceptible cultivars. The main goal of this study, was to determine any induced changes in transcriptional levels of RPL3 (A and B type) genes under stress conditions caused by Fusarium Head Blight (FHB) in wheat. In order to detect expression levels of RPL3 genes, we extracted RNA from both inoculated and non-inoculated spikes of sensitive and tolerant cultivars, during 1, 3 and 7 days post-infection. Quantitative RT-PCRs were performed with A and B type-specific RPL3 primers to detect the expression differences. Primary data indicated a decrease in the genes expression level in Sumai3 and Falat cultivars 24 hours post-infection. On the other hand, increased level of RPL3A transcript in Frontana 7 days post-infection may reveal the role of this specific protein in inducing the resistance in this cultivar.

Fusarium head blight (FHB) is one of the most destructive diseases of wheat causing significant reduction in grain yield and quality. Development of resistant varieties is an effective, economical and enviromentally safe way to control FHB disease. A major QTL (quantitative trait locus) for Fusarium head blight resistance, Qfhs.ndsu-3BS, derived from cv. Sumai 3, has been identified and verified by several research groups via molecular marker analysis. The resistant cv. Sumai 3 was crossed to susceptible cv. Falat, then three backcrosses were followed by one self-fertilization. Three simple sequence repeat (SSR) markers, Xgwm 389, Xgwm493, Xgwm533, were used for marker assisted selection (MAS) in BC1 and BC2 generations.The probability of linkage between markers and Qfhs.ndsu-3BS was calculated using a binomial probability function based on the assumption that a molecular marker at a specific distance from Qfhs.ndsu-3BS in the population would carry the donor-parent allel as a function of the distance between marker and QTL and the number of backcrosses/selfs used in deriving the population. Microsatelite locus Xgwm 493 was significantly associated with Qfhs.ndsu-3BS.