جستجوی مقالات مرتبط با کلیدواژه « antioxidant » در نشریات گروه « اکولوژی »

One of the prominent effects of free radical activity in the body is damage to biological and vital molecules such as nucleic acids, proteins and lipoprotes. So, free radicals, especially reactive oxygen species, play an important role in medical issues. Using compounds that neutralize free radicals, i.e. antioxidants, is a suitable choice to minimize the damage caused by their production. Herbs are recognized as rich sources of antioxidants and consumption of these plants can be effective for human health. The present study was conducted to evaluate the antioxidant property of native medicinal plants including Babouneh shoshi, Anthemis Susiana Nabelek, Ghodoumeh dokhtari, Alyssum campestre, and Kangar, Gundelia tournefortii in Ilam Province, Iran. Total antioxidant capacity was measured using at 570 nm with the enzyme-linked immunosorbent assay (ELISA). The results showed that the total antioxidant capacities of Babouneh shoshi, Ghodoumeh dokhtari, and Kangar are 4.29, 1.01 and 1.25 mmol Fe2+ L-1, respectively. Generally, there are several natural antioxidant compounds in Iranian medicinal herbs which made them as reliable sources for therapeutic purposes. The essential oils of these plants are the source of herbal secondary metabolites with antioxidant and antimicrobial effects that are recommended for their use in the food, pharmaceutical, and health industries.

The purpose of the present study was to investigate the antioxidant and anti-inflammatory properties of glucosamine hydrochloride (G-HCl), glucosamine sulfate sodium chloride (GS-Na) and glucosamine sulfate potassium chloride (GS-K) isolated from the shells of Litopenaeus vannamei obtained from a shrimp processing plant.

G-HCl was synthesized via hydrolysis of chitin with concentrated HCl followed by several sequential decolorization, crystallization and washing steps. Using G-HCl as the precursor, addition of sodium and potassium sulfates at 40 ºC for 1 h resulted in production of GS-Na and GS-K.

The yield of chitin was found 19.9% and those of glucosamine products ranged between 75.5%-82.5%. The HPAEC-PAD indicated the presence of glucosamine monomers, as compared with commercial standard, with different elution time to that of glucose. The appearance of characteristic signals of O-H, N-H and C-O-C in the FT-IR spectra provided further support of glucosamine successful isolation. SEM images and EDX spectra of glucosamines confirmed the elemental compositions of samples and their polyhedral crystalline structures. DSC and TGA thermograms indicated endothermic and exothermic peaks specific to glucosamine products. Relatively low DPPH and ABTS radical scavenging activities and ferric reducing power was obtained for all glucosamine products. all the glucosamine derivatives indicated an anti-inflammatory effect on LPS-simulated RAW264.7 cells.

Glucosamine products showed no cytotoxicity and down-regulated the release of NO in RAW264.7 murine macrophage cells induced by LPS. Overall, the present results indicated the successful production of glucosamine from the waste of L. vannamei processing plant with antioxidant and anti-inflammatory properties.

Dracocephalum kotschyi, an endemic medicinal plant in Iran, has long been used in folk medicine to treat various disorders. This study aimed to determine the phytochemical composition and biological activities of various extracts of leaves and flowers of D. kotschyi. Different solvent extracts (aqueous solution of acetone, methanol, ethanol, and a mixture of acetone and methanol) of D. kotschyi were screened for anti-amylase activity, antioxidant potential, and antibacterial efficiency. Antioxidant effects were elucidated by the methods such as DPPH and FRAP. The inhibitory potential of the extracts against amylase, a key enzyme involved in diabetes, was investigated by the Bernfeld method. The total phenolic, flavonoid, and anthocyanin contents of these extracts were also calculated. Disc diffusion, MIC, and MBC methods were applied to analyze the antibacterial efficiency of the extracts. GC-MS analysis of the essential oil was performed. Additionally, the HPLC method was used for the identification and quantification of caffeic acid. Based on antioxidant assays, the acetonic extract showed the highest antioxidant ability, due to its highest total antioxidant content. Also, the acetonic extract strongly inhibited α-amylase activity. Various extracts of D. kotschyi displayed inhibitory effects against both Gram-positive and Gram-negative bacteria. Analyzing the essential oils of the leaves and flowers of D. kotschyi by GC-MS led to identifying 6 and 19 compounds, respectively. These results suggest that D. kotschyi can be considered a promising source of natural antioxidant, antimicrobial, and anti-amylase agents for managing oxidative damage, as well as pharmaceutical and food purposes.

The purpose of this study was to ascertain the vitamin C content of locally cultivated vegetables in Thailand. As the study's sample veggies, local, conveniently accessible product is regularly consumed by Thais. Vegetables include eggplant, Solanum melongena L., kajon flowers, Telosma minor Craib, lettuce, Lactuca sativa L., butterfly pea flowers, Clitoria ternatea Linn, acacia. Acacia farnesiana (L.) Willd, spinach. Spinacia oleracea L., and zucchini, Cucurbita L. var. cylindica Pans. Fresh vegetable vitamin C levels range from 130 ±1.00 mg kg-1, 340 ± 0.58 mg kg-1, 350 ± 1.00 mg kg-1, 340 ± 0.58 mg kg-1, 440 ± 0.58 mg kg-1, 490 ± 0.58 mg kg-1 and 140 ± 0.58 mg kg-1, respectively. Spinach contained the most vitamin C, with a concentration of 490 mg kg-1 followed by Acacia, lettuce, kajon flowers, butterfly pea flowers and zucchini respectively. However, vitamin C is still necessary for healthy skin and a strong immune system. The body can also get the appropriate daily quantity of vitamin C by eating the right kinds and amounts of fruits and vegetables.

Basil (Ocimum basilicum L.) Lamiaceae is the most important medicinal plants. Much of the essential oils of basil essential oil phenylpropanoids which 90% are included. Important phenylpropanoid compounds include. The experiment was done in a randomized complete block design (RCBD) with factorial arrangements with 3 levels of UV-C Radiation (1/5- 4/5- 10 KJ M-2S-1) and Changes in Antioxidant activity and phenolic compounds were evaluated during five different stages. The amount of Antioxidant activity the gene expression was not affected by ultraviolet radiation treatment, and the expression level increased in control samples compared to other samples Furthermore, phenolic compounds were tested. UV-C treatment at the level of 10 kJ M-2S-1their expression levels increased. 

In this study, the antioxidant properties of hydrolyzed protein from longtail tuna dark muscle with commercial enzymes (alcalase, alkaline protease, and evatase) were investigated.

Protein hydrolysates from tuna dark muscle were prepared by different enzymes Degree of hydrolysis (DH) was performed by TCA technique. The five aliquots at 60, 180, 240, 300, and 360 min were gathered during hydrolysis. The antioxidant activity of aliquots was monitored by in vitro assays (DPPH inhibition ability and Ferric (Fe3+) reducing power).

The antioxidant activities of protein hydrolysate from tuna dark muscle (TDM) increase with increasing time and DH. Alcalase hydrolyzed protein (AHP) generally showed higher antioxidative activity than evatase hydrolyzed protein (EHP) and alkaline protease hydrolyzed protein (APHP). Among the samples (concentration 3 mg.ml-1), AHP at 360 min significantly exhibited the highest ability to scavenge DPPH radical (72.6 %). Furthermore, AHP and APHP significantly showed a minimum IC50 value of 1.1 mg.ml-1 at 240 and 360 min hydrolysis. APHP significantly exhibited the highest ferric reducing power of 0.83 at 300 min and 0.76 at 240 min. AHP and APHP significantly showed the highest ferric reducing power of 0.74 at 360 min (p < 0.05).

This study confirmed that protein hydrolysate from TDM could be a good source of antioxidant peptides. In addition, the antioxidant activity of hydrolyzed protein relay on protease type and hydrolysis condition.

This study aimed to investigate and evaluate the amount of antioxidants activity and total phenolic contents of the plant Garcinia atroviridis Griff. ex T.Anderson. Only the leaves and fruit were used for this study. These samples were washed, dried and grinded, then Soxhlet apparatus and methanol were used for extraction, and the end products were used for the study. Two methods were used for this study, 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assay was used to evaluate the antioxidant activity and the second assay was total phenolic content (TPC) to measure the quantity of phenols in leave and fruit extracts. Four standards were used for DPPH assay including ellagic acid, tannic acid, gallic acid and rutin. Their results were then compared with the two samples results. Twelve concentrations were prepared for the standards and samples. The antioxidant activity was expressed as the efficient concentration needed to scavenge 50% of free radicals. For TPC assay, gallic acid was the standards and six concentrations were prepared for the standards and samples. In both methods, microplate reader machine or spectrophotometer was used to read the absorbance. According to the results of both assays, the fruit extract exhibited higher antioxidant activity and higher total phenolic content compared to the leaves extracts. The efficient concentration of fruit extract was 4 mg mL-1, while in the case of leaves extract, it could not be attained. The mean of gallic acid in 1 mg of fruit extracts was 0.070, while in leaves extract was 0.069. It was also concluded that when the concentration increases, antioxidant activity and total phenolic content elevates as well.

The purpose was to specify the impacts of amoxicillin, cefixime, and metronidazole on sunray cultivar of green bean (Phaseolus vulgaris L .) growth and some of its metabolites, which were cultured in soils with various quantities of heavy metals.

For evaluation of the effects of the antibiotics (i.e., Amoxicillin, Cefixime, and Metronidazole) on plant growth, the Antibiotics were tested in 2 doses of 100 and 200 mg.kg-1 soil, and zero for the control. After 42 days fresh and dry weights of green bean shoots and roots were analyzed, and before harvesting the quantity of Chlorophyll (Chl) a, Chl b, Carotenoid (Car), Flavonoids, Phenol, and Antioxidant activity in P. vulgaris leaves were measured.

Green bean treated with Amoxicillin (200 mg.kg-1) produced the highest root dry weight in mine soil (42.91% increase compared to control) while it treated with cefixime (200 mg.kg-1) produced the highest shoot dry weight at rangeland soil (19.21% increase compared to control). The quantity of Flavonoids (18.91%), Phenol (19.70%), Chl a (37.72%), and total Chl (37.40%) in the leaves of the plant with 200 mg.kg-1 Metronidazole reduced in compared to their controls. The results showed that antioxidant activity in green bean tissues was enhanced in mine soil compared to agricultural and rangeland soils (37.76% and 18.43% respectively).

Though soil contamination with heavy metals and the usage of metronidazole had stressful results on green beans, these were not additive or synergetic. Residues of Antibiotics often enter agricultural soils through animal manure, so it is suggested to use at least decomposed manure to control their stressful effects and the arrival of Antibiotics into the food chain.

Compounds with chemical systems depend on the coumarin architecture have sparked a lot of interest in the scientific community, not only because of their different morphological characteristics, but also because of their wide range of biological properties. In this study, four 7-halomethylcoumarin-4-acetic acid derivatives tagged as YMa-YMd were synthesized by coupling various 3-halomethyl phenols with 3-oxoglutaric acid that prepared in situ from the interaction of citretten and concentrated H2SO4. The acquired coumarin derived compounds were grafted to 5-fluorouracil through amide bond using dichlorosulfoxide as a coupling reagent. The chemical frames of the final conjugated coumarins, named YM1-YM4, were identified and established by analyzing their spectral data gathered from various analytical spectrophotometers, involving FTIR, 1HNMR, and 13CNMR. The potential of the conjugated coumarins to act as antioxidants was investigated by monitoring their ability to trap the free radicals of DPPH. Besides, the chemotherapeutic potential was assessed against two standard tumor-cell lines, named HeLa and MCF 7, using a well-validated technique based on the MTT as a visual indication. The outcomes acquired from these assessments indicated that the synthesized conjugated coumarins have less impact as antioxidizing and cytotoxic agents comparing with the utilized standard drugs. Furthermore, these coumarins showed essentially the same pattern of action against the two cell lines examined, with MCF-7 acquired the most inhibitory effect. Additionally, conjugated coumarin YM1 showed valuable activities as antioxidant and chemotherapeutic agent compared to the other synthesized derivatives. As a result, the authors concluded that the synthesized conjugated coumarins might be used as antioxidant and anticancer agents, with conjugated coumarin YM1 being the most promising. Moreover, the synthesized core might serve as a beneficial framework for developing medicines with potent antioxidant and anticancer properties.

A huge amount of azo-dye wastewater is generated annually by the textile industry. At present, the improper disposal of azo dyes to water bodies causes considerable concern as it can disrupt the ecosystem and, because of their toxicity and carcinogenicity, constitutes a possible environmental and health problem. Because of its cheap, environmentally safe, and sustainable properties, chemical, physical, and biological methods have been used to treat azo-dye wastewater, and biological technology has been recognized as a promising technique. Azo dyes are one of the most pollutant synthetic dyes to the environment. Azo dyes can be transmitted through a food chain and may be associated with problems of toxicity, carcinogenicity, and mutagenesis. In this study, contaminated soil with two Azo dyes, Reactive Blue (RB) and Reactive Red (RR) was used in sorghum planting (Sorghum bicolor L. Moench) using different dye concentrations (0, 0.05, 0.1, 0.2 g Kg-1 soil). Increased concentration of the dye caused significant alterations in the morphological characteristics of sorghum, such as steam length, leaves number and leaf area. There was a slight decrease in the plant content of total chlorophyll and carotenoids, while a significant variation in CAT and SOD enzyme activities was observed by increased dye concentration in the soil for both dyes compared to the control group. In addition, a significant increase in proline content was found at higher dye concentrations.

The objective of the study was; to determine the antimicrobial activity of extracts of Baccharis latifolia (Bl) against Salmonella, E. coli and Listeria of food origin; as well as evaluate the antioxidant activity of these extracts. Extracts of leaves, root, stem and Bl flowers were used. After the analysis of antimicrobial activity against Listeria and Salmonella. After analysis showed that leaf and flower extracts were more effective, while against E. coli, flower and stem extracts. In antioxidant activity, the Bl flower extract at 60 mg/mL had a greater effect with 47.25%. In conclusion, Bl extracts, proved to be a natural alternative to partially inhibit pathogen development

The research was performed to assess the effect of vermicompost and tuber size on the storage behavior of potato during the ambient condition.

The research was consisted of two factors, i.e., factor A: vermicompost level (Vm-4): Vm1: 0 t ha−1 (control), Vm2: 3 t ha−1, Vm3: 6 t ha−1, and Vm4: 9 t ha−1; factor B: tuber size (T-5): T1: 5–10 g, T2: 10–20 g, T3: 20–30 g, T4: 30–40 g, and T5: > 40 g. The research was conducted in a split-plot design where vermicompost levels were assigned to the main plots and tuber sizes to the subplots.

Vermicompost had a significant effect on most of the processing quality contributing parameters of potato. Processing quality parameters increased with increasing vermicompost level irrespective of tuber size. Among the 20 treatment combinations, vermicompost @ 9 t ha−1 with tuber size > 40 g exhibited the highest starch (23.81 mg g−1 FW), antioxidant (478.84 Trolox µMol 100 g−1 FW), polyphenol (96.133 GA mg 100 g−1 FW), and the lowest reducing sugar (0.1020 mg g−1 FW). In respect of ambient storage condition, starch decreased with increasing storage time; reducing sugar gradually increased with increasing storage time up to 40 days after storage (DAS) and after that slightly decreased and finally, potato became non-suitable for both table and processing purpose; antioxidant and polyphenol slightly increased up to 20 DAS and after that gradually decreased with increasing storage time.

Potato growers may use vermicompost for increasing and improving processing quality of potato.

Radon, as naturally occurring radioactive gas, is responsible for 50% of the total background radiations in human. Radon gas is able to enter to human body through eating and drinking. So, measurement of received radiation in the human body is essential. In this study, the presence of radon in milk and its dose was examined. This experiment was conducted using 12 Rayeni goats which have been categorized in a completely randomized design by 4 treatments and 3 replication (Treatment A = Radon-containing water + zero antioxidant, Treatment B = Radon-containing water + antioxidant, Treatment C = healthy water + antioxidant, Treatment D = healthy water+ zero antioxidant). The experiment was prolonged for 60 days. During the experiment the goats were milked every day. The samples were transferred to laboratory to determine the milk compounds and properties, as well as to measure its radon level. Presence of radon in milk was detected using Rad7 device. The averaged radon concentration in milk samples (for treatments A and B) receiving radon was about 126 Bq m-3. There was no significant difference between protein. But there was a significant difference between the percentages of fat, lactose, total antioxidant capacity and the number of somatic cells. Radon did not change pH and Malondialdehyde contents of the treatments. We also determined annual received radon dose per person from drinking milk. It was different among age groups. Newborns were at higher risk of internal radon exposure from contaminated milk. Radon can enter thelivestock milk. According to our findings, with, this radon amount in milk was not higher than the allowable level in valid resources for human health.

Nowadays it is very desirable to investigate and discover new antibacterial and antioxidant agents from natural products and medicinal plants. The current study was conducted to examine the antimicrobial and antioxidant properties of an endemic plant named Caspian saffron, Crocus caspius. After collecting C. caspius and drying them in the shade, ethanol, methanol and hydroalcohol extracts were prepared using maceration method. The amount of phenols and flavonoids measured in this study 2, 2_diphenyl-1_picrylhdrazyl (DPPH) test and disc diffusion method were used to evaluate antioxidant and antimicrobial activities, respectively. According to the results, phenols and free radical scavenging capacity were at highest level in the hydroalcoholic extract and flavonoids at highest level in the ethanol extract. More antioxidant activity of extracts was obtained in the higher concentrations. The highest amount of inhibitor for antimicrobial activity was in methanol extract using Candida albicans. The results indicated the potentiality of C. caspius extract to use as bio-preservatives and antimicrobial agents. However, further investigations are needed in the future in this regard.