جستجوی مقالات مرتبط با کلیدواژه « سودوموناس » در نشریات گروه « باغبانی »

Deficiency or excess of any growth factor leads to morphological, anatomical, physiological, and growth disorders in plants. Drought stress means a lack of water in the plant. This situation occurs when the amount of transpiration exceeds the amount of water absorption. Low irrigation (watering less than the optimal water requirement of the plant) is a strategy to save water, but it affects the plant at a particular stage of growth or the entire season. Biofertilizers develop the root system, changing host plant water relations and plant metabolism. They also activate the defense system, improving access to minerals and tolerance to drought in the host plant, making it environmentally friendly and natural. To manage the prevailing water crisis, one should look for solutions such as reducing water consumption and stopping irrigation at all stages of growth. This is without harming the plant's final performance. In addition, due to the importance of medicinal plants and increasing their biomass, biological fertilizers and the adjustment of drought stress effects have a special place. This experiment was carried out to investigate the effectiveness of Satureja bachtiarica Bunge. against dehydration at different stages of growth. It also investigated the consumption of mycorrhizal fungi and growth-stimulating bacteria to manage plant production with high quality and quantity.

The experiment was carried out in the form of split plots in the form of a randomized complete block design in three replications in the research farm of Islamic Azad University, Tabriz branch, in 2018. The main and sub-factors were included in 4 water stress (I1: without stress, I2: cut irrigation in stem elongation stage, I3: cut irrigation at the budding stage and I4: cut irrigation at 50% flowering stage) and 5 biofertilizer levels (b1: without inoculation, b2: inoculation with Funneliformis mosseae, b3: inoculation with Rhizophagus irregularis, b4: Funneliformis mosseae + Rhizophagus irregularis and b5: Azosprillium+Pseudomonas+Azetobacter), respectively. The harvest was in full flowering stage.

The comparison of the average of irrigation interruptions showed that the most number of leaves, stem diameter, canopy extent, root weight, leaf yield, flowering stem yield, and flowering branch yield belonged to the irrigation interruption at the 50% flowering stage. Inoculation with Funneliformis mosseae mycorrhizal fungus achieved the highest leaf yield (912.06 kg/ha), flowering stem yield (1071.25 kg/ha) and flowering branch yield (1983.32 kg/ha) in inoculation with Azosprillium + Pseudomonas + Azotobacter was observed. The highest percentage of essential oil was found in inoculation with Funneliformis mosseae with an average of 1.76%. The maximum average yield of essential oil at 30.50 kg/ha was observed in the inoculation treatment with Azosprillium + Pseudomonas + Azotobacter. The results of the comparison of the average of the interaction effect showed that the maximum height of the plant and the number of inflorescences with an average of 61.72 cm and 30.46 per plant were found in the interaction effect of the treatment of interruption of irrigation at the stemming stage and inoculation of Rhizophagus irregularis + Funneliformis mosseae, which is about 31 and 39 The percentage increased compared to their lowest value. Stopping irrigation in the budding stage ×Funneliformis mosseae had the highest percentage of essential oil with an average of 2.33%. Stopping irrigation in the stemming stage ×Rhizophagus irregularis had the lowest essential oil percentage. The highest leaf yield (1212.83 kg/ha), flowering stem yield (1494.75 kg/ha), flowering branch yield (2707.58 kg/ha) and essential oil yield (44.39 kg/ha) in the interaction effect of cutting × Azosprillium + Pseudomonas + Azotobacter irrigation was observed at the 50% flowering stage, which increased by 70, 82, 81 and 87% compared to the lowest ones.

The correct management of soil fertility, improvement, and maintenance of soil fertility in modern agricultural systems will provide the nutrients needed by the plant and thus enhance the yield. Therefore, in recent years, many studies have been conducted on biological fertilizers. Mycorrhizal fungi are essential factors in the stable plant-soil system, which coexists with more than 85% of plants. Drought stress is one of the most influential environmental factors in plant growth and causes morphological, physiological, and metabolic changes. In addition, biofertilizers increase plant access to nutrients and produce and direct hormones involved in the growth of the root environment. They also increase stress tolerance and enhance yield. Considering the arid and semi-arid conditions of the country and the increase in demand for medicinal plants and Satureja bachtiarica spices, it is recommended to stop irrigation at the 50% flowering stage and use Azotobacter, Azospirillium, and Pseudomonas biofertilizers to obtain the maximum yield of leaves, flowering branches, and essential oil.

The objective of this study was to investigate the effects of biofertilizers and nutrition through a nutrient solution (base Hogland) on quantitative and qualitative characteristics of Begonia semperflorens (two varieties, sprint rose and senator deeprose). The factorial experiment was carried out based on randomized complete block design (RCBD), with twenty treatments in three replications. Treatments include: nutrient solution (base Hogland), Pseudomonas fluorescens strain 169, Azotobacter chroococcum strain 12 and compound Pseudomonas and Azotobacter with different percentages of chemical fertilizer and two varieties of Begonia. Non-inoculated with nutrient solution and bio-fertilizer were considered as control. The results showed that the highest leaf area (36.69 mm2) was observed in Pseudomonas and Azotobacter and 75% of the nitrogen treatment. Maximum shoots fresh weight (117.18 gr) and maximum shoots dry weight (4.12 gr), were seen at the treatments of Pseudomonas and Azotobacter and 75% of the Nitrogen and Phosphorus. Most phosphorus in shoots (0.34%) was related to the treatments of Pseudomonas and Azotobacter and 100% phosphor and the highest chlorophyll of leaves (13.12 mg per gr of fresh weight) were related to the treatments of Azotobacter and 100% Nitrogen. Treatments of Pseudomonas and Azotobacter and 75% phosphor with 41.38 mg per gr of fresh weight, had the highest amounts of anthocyanins of petals. Overall, treatment of Pseudomonas and Azotobacter and 75% nitrogen, and then base Hogland treatment were the best treatments. Thus, it was shown that Pseudomonas and Azotobacter combination with reduced doses of nutrient solution, led to significant improvement of quantitative and qualitative characteristics of Begonia. In addition, two varieties of Begonia (senator deeprose and sprint rose) in response to similar treatments, showed different reactions.

The tendency to produce aromatic and medicinal plants and the demand for natural products is increasing especially in the world. Ecological agriculture (Agroecology) of medicinal plants guarantees their quality and reduces the possibility of negative effects on their quality and yield. Evaluation of different plant nutrition systems is one of the most important principles in the production planning of medicinal plants in order to achieve high yield and quality of effective ingredients. Proper fertilizer management is one of the most important factors in agricultural production. Identification of fertilizers which are compatible with nature and are suitable for plant growth could have favorable effects on quantitative and qualitative indices of the plant. Organic fertilizers are genuine and risk-free products that could be suitable for agricultural sustainability. Soil organic matter improves fertility and water retention, provides proper ventilation, long-term storage of plant nutrients and microorganisms, so replacing chemical fertilizers with fertilizers such as vermicompost and biofertilizer can reduce the above-mentioned effects. Biofertilizers are one of the operations that is nowadays in accordance with the principles of sustainable agriculture for soil fertility. Biofertilizers include the compost of one or more types of useful soil organisms or as metabolic by-products of these organisms that are used to provide the plant with the nutrients it needs in an agroecosystem.

In order to investigate the effect of biochar, organic and biological fertilizers on the quantity and quality of Ocimum bacilicum L., a factorial experiment was done in a randomized complete block design with three replications. Experimental treatments included two levels of biochar (0 and 20 ton ha-1) and five levels of fertilizers (vermicompost (10 ton ha-1), Mycorrhiza, Azotobacter, Pseudomonas and no fertilizer application). Prior to planting sweet basil seed, biofertilizers and vermicompost were mixed with soil to the depth of 15 cm. Mycorrhizal fungi were mixed with seed. Azotobacter and Pseudomonas bacteria were also incubated with seeds (seed inoculated). It should be added that in this study no chemicals were used to control weeds and pests.

According to the results, the highest plant height and number of main branches were obtained in biochar application with vermicompost (49.37 cm and 7, respectively), which was significantly better than other treatments. The interaction effect of biochar and fertilizer had a significant effect on fresh and dry weight of leaf and stem. Mean comparison of treatments showed that the highest leaf and stem fresh weight were obtained in biochar + Pseudomonas application (792 and 876 g m-2, respectively), which had no significant difference with biochar + vermicompost; and also, the maximum leaf dry weight and stem dry weight were obtained in biochar with Pseudomonas application (166 and 175 g m-2, respectively). The interaction between biochar and fertilizer on phenol, flavonoids and antioxidant activity of basil showed a significant differences between treatments.

In general, the results showed that the use of biochar and biological and organic fertilizers could be effective in both the quality and quantity of sweet bail, where, the use of the bacterium Azobacter and Pseudomonas with biochar had the greatest effect on the quality and quantity of sweet bail. Finally, it could be concluded that the combined use of biochar with biological fertilizers had significant impacts on the quantitative and qualitative traits of sweet bail in ecological or low input agricultural.

In order to study the effect of nitrogen and phosphorus supplier bacteria on tomato transplants characteristics, a factorial experiment in a completely randomized design with three replications, was conducted in a greenhouse of Gorgan University of Agricultural Sciences and Natural Resources, in March 2016. The first factor was biological fertilizer application at five levels (control, seed treatment of azosperilium, seed treatment of Pseudomonas, Azosperilium soil use, Pseudomonas soil use), and the second factor was irrigation at two levels (four times a day and two times a day). In this experiment, Azospirillium brasilense strain (P) and Pseudomonas fluorescens strain (p-169) were used. The results showed that the use of biofertilizers of Azospirilium brasilense and Pseudomonas fluorescens in soil application compare with control, improved germination percentage, stem length, stem and leave dry weight, root fresh weight, decreased the time to appearance of sixth leaf and increased anthocyanins in the tissue (1.67, 2.9, 19.05, 9.25, 3.38 and 18.42%), respectively. So, Azosperilium brasilense and Pseudomonas fluorescens as soil use can be used in normal irrigation regimes to improve the quality, shorten the nursery period, reduce water stress and improving seedling tolerance to the transmission stress in the main field, in the production of tomato transplants.

Organic amendments not only promote soil quality but also indirectly facilitate the establishment of bacteria. A greenhouse experiment was conducted to investigate the effects of organic substrates and plant growth promoting rhizobacteria inoculation on morphological characteristics of lettuce (Lactuca sativa L. Cv Rabicon.) in a factorial arrangement based on completely randomized design with four replications. Experimental treatments included organic fertilizers (vermicompost 20% v/v, spent mushroom 10% v/v and soil) and seed priming with promoting growth regulating bacteria (Pseudomonas putida P10, Pseudomonas fluorescence R159 and R150 and non-inoculated). At the end of growth stage, morphological traits (plant height, leaf number, crown diameter, leaf area, root length and volume, shoot fresh and dry weight), photosynthesis pigments (chlorophyll a, b, total and carotenoid), vitamin C, phenol, flavonoid and nitrate concentration were measured. The results indicated that the simple effects of substrate and bacterial strains inoculation were significant on traits including plant height, crown diameter, leaf area, root length and volume, shoot dry and fresh weights, chlorophyll a, b, total and carotenoid, vitamin C, phenol and flavonoid. Interaction of both factors had significant effects on leaf area, root length and volume, chlorophyll a and phenol. However, plants treated with vermicompost and Pseudomonas fluorescence R159 had higher values for both fresh and dry weight, phenol content and chlorophyll. Although some traits were higher in plants treated with compost and Pseudomonas putida P10 or vermicompost and Pseudomonas putida P10, but finally it seems that Pseudomonas fluorescence R159 has played a higher role in the final quantity and quality of lettuce.

In order to study the effect of biofertilizers, on the yield, quality and antioxidant capacity of tomato fruit, an experiment based on randomized complete block design with 9 treatments and 3 replications was carried out at Khalatpoushan station, Faculty of Agriculture, Tabriz University. seeds of Super Chief tomato variety were inoculated with individual and combined treatments of potassium releasing bacteria (Pseudomonas sp. S19-1, Pseudomonas sp. S14-3), phosphate solubilizing (P. putida Tabriz, P. fluorescens Tabriz) and nitrogen fixing (Azospirillum sp. Acu9, Azotobacter sp.) In the nursery cultivation. In addition, control treatments were performed, without bacterial inoculation and fertilization (control 1) and control treatment without bacterial inoculation and with fertilization (macro elements) based on the soil test analysis (control 2) compare the results. Traits assessed include: total yield, economic yield, non-economic yield, the number of total fruit, average weight of fruit, fruit dry matter, lycopne, vitamin C, total acid and fruit potassium. Results showed that inoculation with bacteria had significant effect on yield, potassium content of fruit, acidity of fruit, dry matter, vitamin C and lycopene content and the highest yield, potassium content of fruit, acidity and fruit dry matter were observed in potassium releasing bacteria treatment. The highest amount of vitamin C was observed in the potassium releasing and phosphate solubilizing bacteria combined treatment while the highest amount of lycopene was obtained in the potassium releasing and phosphate solubilizing and nitrogen fixing bacteria combined treatment. According to the above results, potassium releasing and phosphate solubilizing and nitrogen fixing bacteria as a biofertilizer could be employed to improve yield and quality characteristics of tomato.

In order to investigate the effects of Plant Growth Promothing Rhizobacteria (PGPR) on growth, yield, flowering and biochemical content of marigold (Calendula officinalis L.), an experiment was carried out in a randomized complete block design with five treatments and four replications during the 2015-2016 in the Faculty of Agriculture of Shahid Chamran University of Ahvaz. The treatments included Entrobacter closea R13, Entrobacter closea R33, Pseudomonas sp., chemical fertilizers (NPK), and control (no fertilizer). The results of the experiment showed that the highest flower number per square meter, leaf number, stem branches, receptacle diameter, and capitol diameter were observed in E. closea R13, while the highest capitol fresh and dry weight was found in E. closea R33. Moreover, the most leaf phosphorus and nitrogen content, and leaf chlorophyll and carotenoids content were observed in the plots incubated with E. closea R13. The highest and lowest total phenolic content was found in the Entrobacter treatment and control, respectively. Mean comparison of treatment effects showed that the effects of E.closea R33 were similar to E. closea R13 in most parameters and there was no significant difference between them. According to the positive and additive effects of PGPR on calendula plants, the PGPR could be recommended as an alternative to chemical fertilizers to reduce the consumption of chemical fertilizers and guaranty the production of medicinal plants, free of chemicals.

In recent decades, excessive use of chemical fertilizers causes environmental problems such as water resource pollution and decrease in soil fertility. Organic matters are excellent sources of plant-available nutrients and their addition to soil could maintain high microbial populations and activities. In crop studies, Prabha et al. (2007) reported that there was excellent plant growth as well as yield in garlic plants that received vermicompost as nutrient in the field (28). Recent studies confirmed that, a number of bacterial species mostly associated with the plant rhizosphere, are found to be beneficial for plant growth, yield and crop quality. Therefore, the objective of this study is to investigate the growth promoting effects of organic and bio-fertilizers on tomato growth and yield. A factorial experiment in randomized complete block design with three replications was conducted at the College of Agricultural, University of Shahrood in 2011. Geographically, the site is located in Bastam (36° 25’E, 54° 58’N, 1349 m a.s.l.).The climate of this region is semi-arid. Treatments included three levels of organic fertilizers: vermicompost (1300 kgha-1), cow manure 3350 kgha-1), and control, biological fertilizer in four levels (Pseudomonas putyda, Pseudomonas fluorescens, Azotobacter chrococcum and control). The bacterial suspension for each species was applied at a rate of 3 liters per hectare. Metribuzin herbicide (wettable 80% powder) was used at a rate of 1000 gr. ha-1. Petopride No. 2' variety of tomato (Lycopersicon esculentum Mill.) was used in the present experiment. At the time of harvesting, the plant characteristics namely lengths and diameter of stem, number and weight of fruit, weight of stem and leaf were also registered. Statistical analyses of data were performed with statistical software Mstatc. Significant differences between means refer to the probability level of 0.05 by LSD test. There sults showed that main interaction effects of bio and organic fertilizers were significant (P<0.05) on bacterial colonization. The bacterial population increased with application of vermicompost and biofertilizers than other treatmentssignificantly (Figure 1).Chemical analysis showed that vermicompost had a lower pH, and higher amounts of nitrogen, phosphorous and organic matter compared to the cow manure and soil (Table. 1). Thus, it seems that vermicompost has been able to provide an appropriate bed for the growth of bacteria. The results indicated that the dry weight and height of tomato stems was improved by 28.5 and 4.7 percent, respectively by application of vermicompost compared to the control treatment (Table 4). The number of tomato fruits increased by the application of vermicompost and cow manure by 29.2 and 19 percent compared to the control, respectively. Also, the yield of tomato was increased by 15 and 10 percent compared to the control treatment by the application of vermicompost and cow manure, respectively. Federico et al. (2007) reported that the yields of marketable tomato fruits per plant increased by 1.8 times in response to a 1:1 vermicompost to soil mixture compared to the control treatment (10). An excreta of earthworm is rich of micro-organisms, especially bacteria and contain large quantities of plant hormones (auxin, gibberellin and cytokinin) that in low concentrations significantly affect plant growth and development 3). Phosphorus is an essential nutrient for plant growth anddevelopment and is one of the most important elements in cropproduction. Despite its wide distribution in nature, it is a deficientnutrient in most soils, especially in soils which have a high Pfixationcapacity. Since a substantial amount of any applied Pfertilizer is rendered unavailable frequent applications ofsoluble forms of inorganic P are needed to maintain adequate Plevels for plant growth. It is a wellknown fact that aconsiderable number of bacterial species, mostly those associatedwith the plant rhizosphere, are able to exert a beneficial effectupon plant growth. Phosphate solubilizing microorganismsrenderthese insoluble phosphates into soluble form through the processof acidification, chelatiation and exchange reactions. In our experiment, probablyincreasing the availability of P and N fertilizers has improved the growth and yield of tomato plants. Azotobacter chrococcum increased dry weight and height of stem and yield of tomatoes by 32.14, 7.23 and 21.2 percent, respectively compared with the control treatment (Table 2). In similar results, the use of N2-fixing bacterial stimulated yield and quality parameters of sugar beet and barley (8). The positive effects of Azotobacter bacteria on the yield and growth of tomatoes were explained by promoting abilities of these bacteria for auxin and cytokinin production, N2-fixation, phosphate solubilization and antimicrobial substance production (8). The yield and plant growth enhancement effects of A. chrococcum used in this study on tomato could be explained by similar reasons. The results of the present study suggested that inoculation of tomato with biofertilizers and the use of organic fertilizers such as vermicompost and cow manure have a potential to increase the growth and yield of tomatoes. Considering environmental pollutions with excessive use of synthetic fertilizers and high costsin the production of N and P fertilizers, the bacteria tested in our study maybe a promising alternative as a bio-fertilizer for fruit and vegetable production in sustainable and organic agricultural systems.

There isn’t balanced between production and consumption when population increased due to traditional agriculture replaced to the modern agriculture. Evaluated of agriculture knowledge in genetic and biotechnology with using fertilizer and chemical pesticide can be facilitated agriculture production and also can be increased food production in increased countries but it disturb equality of ecosystem biology and also using these kind of toxic material and hormones such as nitrate and cadmium change industrial agriculture to agronomic agriculture. Here we applied randomized complete block design with three replications in a field of Karaj Azad University. We used vermicompost (1 kg/m2 for case and control)¡ Animal manure (3 kg/m2 for case and control)¡ Pseudomonas (10 ml/100gr seed for case and control)¡ and Azotobacter (10 ml/100gr seed for case and control) as a four treatment factors. The analyzed trial were sugar¡ hormones (auxin¡ gibberellin¡ cytokinin)¡ biomass¡ ear yield¡ grain fresh yield. The result showed that treatments were significant on total. Application total treatment increased trials than no application. Azotobacter and pseudomonas have more significant effect on hormones (auxin¡ gibberellin and cytokinin) than other treatments.

Stevia rebaudiana Bert. is a medicinal plant، mostly utilized as a suitable sweeter for diabetic patients. In this research، the effect of single or co-inoculation of Arbuscular Mycorrhiza Fungi (AMF) and bacteria (Bacillus polymixa، Pesudomonas putida and Azotobacter chroococcuom) on regenerated plantlets in tissue culture was investigated in intervals of 15، 30، 45، 60 days after planting. Results showed that in comparison to control، inoculation with single and triple treatments significantly increased length، fresh and dry weight of aerial parts and stevioside content until 60 days after planting while the best enhancement was observed in dual inoculations especially in Glomus+Azotobacter treatments. Interestingly، in dual or triple inoculations، maximum relative growth rate was obtained in 15-30 days and reduced in 30-45 days after planting that was in accordance with their flowering time but in single inoculation maximum relative growth rate was available in 30-45 days and with promoting of flowering phase in 45-60 days declined severely. However، maximum stevioside accumulation rate was obtained 30-45 days after planting in all treatments and in 45-60 days after planting reduced severely in single or triple inoculations and more slightly in dual inoculations. Therefore، microorganisms affect relative growth rate، phenology and stevioside accumulation pattern in stevia.

Nowadays, drought stress and environmental pollutions due to irregular consume of chemical fertilizers, are the most important problems in agricultural productions. This study aimed to examine the bacteria Pseudomonas on vegetative characteristics of canola seedlings under drought stress in the Seed Technology Laboratory, Faculty of Agriculture University Ramin was conducted in 2011. A factorial experiment in completely randomized design with three replications and 12 treatments were performed. The first factor Pseodomonas fluorescence strains of bacteria at three levels: control (no inoculation with the seed), 108p and 169 p and the second factor, 4 levels of drought stress were including zero (distilled water), -0.3, -0.6 and -0.9 MPa. The results showed that the effects of drought stress was significant on all traits measured. Pseudomonas aeruginosa on length of shoot and root and shoot dry weight and had a significant effect. At the highest levels of stress (-0.9MPa), shoot dry weight was hot 0.9 amount compared to the treatment of stress was 62 percent. The inoculation of seeds with Pseudomonas fluorescens bacteria p 169, shoot fresh weight to the value of 68g was found that compared to non-inoculated treatment was 15 percent.Generally, the use of fluorescent Pseudomonas strains of bacteria could reduce the effects of drought on the properties of canola seedlings.

In order to investigate the effect of integrated management of phosphorus (P) fertilizer and phosphate solubilizing microorganisms on nutrients concentration and yield of two barley cultivars، a factorial greenhouse experiment was carried out based on randomized complete blocks design with three replications in 2011. Treatments consisted of two barley cultivars (Bahman and Fasih) and five types of P fertilizer management (seed inoculation with Pseudomonas fluorescens strain 103 + 100% recommended P fertilizer، seed inoculation with P. fluorescens strain 103 + 75% recommended P fertilizer، seed inoculation with P. fluorescens strain 103 + 50% recommended P fertilizer، seed inoculation with P. fluorescens strain 103 + no use of P fertilizer، and no inoculation and no use of P fertilizer as control). The results showed that increasing P fertilizer application increased significantly the plant height، main stem diameter، chlorophyll a and b and biological yield of the two barley cultivars. The highest main stem height، chlorophyll b content، nutrients concentration and plant dry weight was obtained at seed inoculation + 100% recommended P fertilizer treatment. Maximum stem diameter and chlorophyll a was observed at seed inoculation + 75% recommended P fertilizer. Also، Bahman cultivar had the highest forage production as compared to Fasih cultivar. According to the results، it can be stated that integrated management of growth promoting bacteria and improves yield and nutrients’ concentration.

In order to evaluate the effects of different organic and biological fertilizers on quantity and quality of essential oil of Fennel an experiment was conducted in a completely randomized block design with three replications. The experimental treatments were two organic (compost and vermicompost) and two biological (Pseudomonas putida and Azotobacter chroococcum) fertilizers, their all twin combinations (Ps. putida + A. chroococcum, Ps. putida + compost, Ps. putida + vermicompost, A. chroococcum + compost, A. chroococcum + vermicompost and compost + vermicompost) and control (without any fertilizer). There were significant differences between different treatments in terms of seed essential oil percentage, essential oil yield; anethole, fenchone, limonene and estragole content in seed essential oil. Results showed that the highest and lowest percentage of essential oil contents were obtained in control (% 2.9) and A. chroococcum + vermicompost (% 2.2) treatments, receptivity. The highest essential oil yield (29.9 l/ha) and anethole content of essential oil (69.7%) and the lowest fenchone (6.14%), limonene (4.84%) and estragole (2.78%) contents of essential oil were obtained in compost + vermicompost treatment. Essential oil yield and percentage of anethole content in essential oil were significantly higher in all organic and biological treatments compare with control. The highest of fenchone, limonene and estragole essential oil content were obtained in control treatment.

AbstractTuberose is one of the most important cut flowers in Iran and the world. In this experiment, effect of microelements Fe, Zn, Cu and Mn on inflorescence length, inflorescence bunch length, number and diameter of floret, diameter of inflorescence bunch and vase life of tuberose flowers were studied. This study was based on four separate completely randomized experimental design with four replicate and four concentrations include: 0, 2, 4 and 6 mg/lit for each microelement. The fertilizers were sprayed in three stages. The first stage was at eight leaf stage (after 30 days). The second stage was, one month later from first stage and the third stage was one month after second stage of plant growth. The results showed that the concentrations of 6 mg/lit of Fe SO4 on inflorescence length and floret number, 2 mg/lit on bunch length and 4 mg/lit on floret opening was significant (P≥1%). The concentrations of 2 mg/lit of Cu SO4 on length of flower bunch and floret number, 6 mg/lit on floret opening percentage and inflorescence diameter and 4 mg/lit on length of inflorescence was effective. Almost all traits were affected by Mn SO4 with 6 mg/lit concentration and vase life reached to 12.4 days.