جستجوی مقالات مرتبط با کلیدواژه « آنزیم کاتالاز » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

To determine the effect of seed deterioration on germination, biochemical and enzymatic activity of buckwheat, an experiment was conducted as factorial based on completely randomized design in Seed Science and Technology Laboratory of Shahed University, Iran. The treatments consisted of ageing duration (non-aging, 2, 4 and 7 days) and seed moisture content (10, 20 and 30%). Parameters such as germination percentage, mean germination time, electrical conductivity, lipid peroxidation and catalase activity were measured. The effect of aging duration, seed moisture content and their interaction on traits were significant. Germination percentage, mean germination time, electrical conductivity, lipid peroxidation, and catalase enzyme activity were significantly affected by aging duration and seed moisture content. The highest germination percentage and catalase enzyme activity in aged seeds was observed during two days with 20% moisture content. Mean germination time, electrical conductivity and lipid peroxidation in aged seeds for seven days was in the highest level in seeds with 30% moisture content. Seed moisture content and aging duration increased the mechanisms involved in seed aging and cell membrane phospholipids affected by deterioration, which increased the electrical conductivity and lipid peroxidation and reduced germination and catalase enzyme activity. To prevent buckwheat seed deterioration in long and short term storage, it is recommended that seed moisture content is kept about 10%.

In this study, the induced responses of Jam chickpea in cold acclimation and cold stress temperatureshave been assayed by detecting electrolyte leakage index (ELI), malondialdehyde peroxidation of membrane lipids (MDA) and the activity of catalase (CAT) enzyme. After exposing plants into cold acclimation situation, they showed more readiness in facing up zero temperature and more clearly in facing below zero cold stress. The results have shown a potent variety in the level and speed of cellresponses in this genotype and the extent of CAT activity, as cell defense system, is matched with ELI and MDA result, as damage indexes. Detecting plants in recovery phase have shown that the increasedtolerance in cold acclimation is a temporary situation and after passing cold stress, not only CAT activitywill decrease but also the amount of damage in cell membrane will decline. So, inducing cold acclimationtreatment increase plant tolerance in cold stress and enhance plant survive and inducing this treatment can cause plant recovery even after sever cold stress.