جستجوی مقالات مرتبط با کلیدواژه "remap" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

In the current investigation, inter retrotransposon amplified polymorphism (IRAP) and retrotransposon microsatellite amplified polymorphism (REMAP) markers were used to study the insertional polymorphism of retrotransposon families Tvv1, Edel, Vine1, Gentil and Huben and genetic diversity in 67 grape (Vitis vinifera L.) cultivars and wild genotypes. Totally, 103 loci generated using 2 IRAP and 13 REMAP primers which 83 (80.58%) was polymorphic. The mean of Nei’s diversity index for primers ranged from 0 (Gentil-A7) to 0.38 (Tvv1-808), averaging 0.27 for the studied grape collection. The Dice similarity coefficients varied from 0.57 (H6 and Dastarchin) to 1 (Rasha-SeyahMamoli) with a mean value of 0.78. The maximum values of Nei’s diversity index (0.32), number of effective alleles (Ne=1.54) and Shannon’s information index (I=0.47) was for retrotransposon Tvv1, showing its high activity and insertional polymorphism in the studied grape collection. Cluster analysis using Dice similarity coefficients and UPGMA algorithm grouped cultivars in 6 main clusters and differentiated cultivated and wild grapes. The UPGMA classification of the studied cultivars was confirmed using model-based methods, implemented in software Structure 2.3.1. It was concluded that used retrotransposon families especially Tvv1 are transpositionally active and could make insertional polymorphism in grape genome; hence molecular markers developed based on this retrotransposon could be used in grapevine genotypes and cultivar identifications.

The aim of this study Investigating the genetic variation in the collection germplasm Sistan grapevine native and non-native cultivars using REMAP marker. To study the genetic diversity of grapevine native and non native cultivars of Zahak research station in Sistan, 33 grape varieties (Vitis vinifera L.) were evaluated by by 7 primer combination REMAP combination ISSR primers and retrotransposons based primer from of Ty1-copia and Ty3-gypsy families. The observed polymorphism for markers MS1 / Gret1 Ra, MS5 / Gret1Rb in both native and non-native varieties were 80 and 75%, respectively. The results revealed that the Gret1 retrotransposon has more transcription and translocation trough the genome. Cluster analysis based on Jaccard's similarity and UPGMA algorithm showed that the population is divided into two groups at (0.44) similarity; one group with twelve sub-groups and one group with one varieties. The results of principal component analysis extracted six components that explained 50.44 % of the total variation among the studied population.