جستجوی مقالات مرتبط با کلیدواژه "میزان اطلاعات چندشکلی" در نشریات گروه "زراعت"

Yield stability and genetic variation in 17 salt-tolerant bread wheat promising lines and three cultivars; Arg, Bam and Ofough were evaluated in field trials as well as by molecular assay. In field, yield trials of genotypes was carried out in saline soils of Melishbar (Yazd), Amirabad (Birjand), Roudasht (Isfahan), Ekhtiarabad (Kerman) and Zahak (Zabol) agriculturar research stations, Iran, in 2012-13 and 2013-14 cropping cycle. To evaluate genetic variation among genotypes, 18 SSR markers that were previously mapped on salt-tolerant quantitative trait loci (QTLs) were used. Combined analysis of variance revealed significant differences for genotype main effect and year × location, year × genotype and year × location × genotype interaction effects. Grain yield comparison of genotypes using least significant difference(LSD) test showed that lines 14, 16, 11 and 5 with grain yields of 4.649, 4.517, 4.441 and 4.300 tha-1 had higher grain yields among genotypes. Different methods of stability analysis revealed that genotypes 11, 15, 16 and 20 (cultivar Ofough) had higher yield stability compared to the other genotypes. Cluster analysis of molecular data using UPGMA method and principal component analysis (PCoA) classified studied genotypes into four major groups. Results of cluster analysis based on molecular data were not in agreement with the results of field experiments. Results of Mantel’s test indicated that correlation between grain yield under salinity stress conditions and their allelic diversity was about 0.13 and not-significant. Despite this weak correlation, SSR markers could greatly reveal genetic variation among wheat genotypes. This genetic variation can effectively be used in wheat breeding programs for salt tolerance in Iran.

West part of Iran is considered as one of the center of diversity for Aegilops triunsialis، therefore، study of its genetic diversity in this area is extremely important regarding evolution of this plant. The genetic variation among 40 accessions of Ae. triunsialis collected from west of Iran were studied using 12 ISSR markers. DNA was extracted، concentrations adjusted and five samples from each accession were bulked. Totally، 118 polymorphic bands were produced using 12 primers that UBC817 primer with 16 and then UBC811 and UBC823 with 14 bands had the maximum number، while UBC825 with 4 bands had minimum polymorphic bands. The PIC value varied from 0. 23 to 0. 40 and the MI value between 1. 1 and 4. 6. Principal Coordinates Analysis showed that the first two could explain the total variation of 23. 72 percent. Cluster analysis using Neighbor Joining، placed the 40 accessions in three clusters that each contain 13، 9، and 18 members، respectively. Discriminate Function via Fisher''s linear confirmed the validity of clustering analysis result (95%).

The first step in breeding programs is detecting the genetic diversity in breeding materials. Molecular markers help to reduce costs and time in breeding programs. Genetic variation in 49 genotypes of tobacco were studied using 12 ISSR markers, 3 Retrotransposon markers and the combination of ISSR and Retrotransposons. One hundred forty seven polymorphic bands were produced using 16 primers. Tos-2 primer with 16 followed by UBC811 and TOS-1 with 14 bands had the maximum and UBC825 and TOS- 3 with 4 band had the minimum polymorphic bands. PIC value varied between 0.27 to 0.46 and MI between 1.15 to 5.87. Principal Coordinates Analysis showed that the aforementioned values could explain total variation of 16.75 percent. In this study, cluster analysis using UPGMA, placed 49 genotypes in five cluster groups. Groups include 9, 4, 1, 13 and 22 genotypes, respectively. Discriminate Function via Fisher's linear (79.6 percent) confirmed the validity of clustering analysis result.