جستجوی مقالات مرتبط با کلیدواژه "rapd" در نشریات گروه "زراعت"

Eighteen pulm cultivars including ten Iranian native and eight foreign cultivars were evaluated based on 36 parametric and non-parametric characteristics and 15 RAPD primers. The results of analysis of variance showed significant differences among the cultivars for all characteristics except the petal color. Dissimilarity matrix revealed that two cultivars Mashhad 4 and Dirras-e-Seif and two other cultivars Ontario and Barbang were distinctly different considering their quantitative and qualitative characteristics. Cluster، constructed from morphological data in distance of 25، classified cultivars into two major groups. Cluster analysis could differentiate the Iranian and foreign cultivars in some extents. Molecular data generated a high percentage of polymorphic bands (99. 04%)، and the similarity matrix of these data showed the highest similarity between Emprofrance and Bokharaye Marghob (71%) and the lowest similarity between Shiro and Bokharaye Mamoli (9%). The cluster constructed from molecular data clustered cultivars from 0. 15 to 0. 85 genetic similarity coefficient. In the similarity distance of 0. 26، the cultivars were divided into three groups where the Dirras-e-Seif cultivar exclusively comprised an indipentent cluster and was distinguished from other cultivars. In valuation of plum cultivars based on morphological and RAPD primers، although Iranian and foreign cultivars were clearly differentiated، but in some cases they showed some differences.

The fragrance and flavour is substantially important in rice commerce. Thereby, it is rational to consider the different aspects of this trait attentively. Hence, an investigation was conducted to study the allelic status of fragrance locus in aromatic rice cultivars Sang-tarom, Basmati 370 and Della. The F2 populations of three crosses among these cultivars were molecularly tested by random amplified polymorphic DNA marker OPAG-08 that is closely linked to rice fragrance gene and in PCR amplifies the 800 bp amplicon in aromatic cultivars, to study the inheritance of fragrance in F2 populations. Based on molecular results, the 800 bp amplicon was produced in 75% of F2 offspring suggesting that at least one identical allele exists in parental cultivars. In the other hand, the amplicon was not produced in the rest of F 2 offspring that reveals the presence of different allele(s) in that locus in parental cultivars. Therefore, regarding the segregation in F2 population, it seems that fragrance locus in these cultivars is controlled by different alleles leading to segregation in their F2 populations.

Oilseed rape (Brassica napus L.) is one of the world ’ s main sources of vegetable oil. The quality of the oil derived from oilseed rape is determined by its fatty acid composition. Breeding oilseed rape for enhanced oleic acid is one of the most important breeding programs that increases oil shelf life. In order to identify molecular markers associated with high oleic acid, a doubled haploid population derived from a cross between the lines T099-5318-20 and DH12075, RAPD and ISSR markers and bulked segregant analysis method were used. Six markers were found to be associated with oleic acid. The markers UBC 2830 and UBC 5381650 accounted 43% and 34% of genetic variation for oleic acid, respectively. In order to make SCAR marker, UBC 2830 was cloned and sequenced. From sequenced information, specific primers were designed. SCAR marker produced a 399 bp fragment linked to oleic acid. The markers identified in this study and the SCAR marker should be useful tools for the selection of high oleic acid genotypes in oilseed rape breeding programs.

In this study 58 isolates of Fusarium moniliforme which causes rice foot rot disease were collected from infected rice seeds. The morphological characteristics and pathogenicity of these isolates were determined in laboratory and greenhouse in the International Rice Research Institute (IRRI). Besides, by RAPD(Random Amplified Polymorphic DNA) technique using primer J-11 from operon kit by sequences of "5-ACTCCTGCGA-3" the randomized portion of the DNA of each isolate was amplified. Using cluster analysis at 25% similarities, the isolates were classified as follows: from point of the growth rate of fungi after 4 days in Petri dishes into 8 groups from point of the number of days that fungi reached to the 90 mm diameter in one group from point of the effect of isolates on the length of seedlings into 5 groups from point of the genetical pattern into 13 groups. The characteristics measured in the laboratory or greehouse for each isolate did not significantly differ with the mean of each group using t-test analysis. Therefore, it could be concluded that molecular markers by RAPD technique can be used in differentiation of morpological and pathogenicity of Fusarium moniliforme isolates. The positive significant correlation coefficient (r=0.54) was observed between the growth rate of fungi in petri dishes and its effect on the length of seedling.