جستجوی مقالات مرتبط با کلیدواژه « بومادران » در نشریات گروه « کشاورزی »

Animal nutritionists are looking for alternative compounds with the ability to improve the fermentation process. Probiotics and medicinal plants can be mentioned among these alternative compounds. Yarrow and ginger can be mentioned among medicinal plants that have antimicrobial properties. The aim of this study was to investigate the effects of yarrow and ginger powder on the digestibility and gas production of sainfoin and alfalfa hay in laboratory conditions.

For this purpose, the in vitro digestibility of sainfoin and alfalfa hay and a mixture of 50% alfalfa and 50% sainfoin were measured due to the use of yarrow and ginger powder by Tilley and Terry's modified Holden method. So that different levels of yarrow and ginger powder at three levels of zero, 1.5 and 3% were added to 100 ml syringes containing alfalfa, sainfoin, and a mixture of alfalfa and sainfoin, and the amount of gas produced by incubating the syringes at times 2, 4, 6, 8, 12, 24, 48, 72 and 96 hours of measurement and the resulting data were analyzed in a completely randomized design.

The results showed that the addition of 3% of ginger and Yarrow powder in sainfoin hay increased the digestibility of dry matter from 55.71 to 68.76 and 74.65% (P=0.0458), the digestibility of organic matter in dry matter from 52.62 to 64.95 and 62.09 percent (P=0.0468) and metabolizable energy increased from 8.42 to 10.39 and 9.93 MJ kg-1 DM (P=0.0489) by Holden's method, respectively. While the use of ginger and yarrow powder did not have a significant effect on the digestibility of organic matter, the digestibility of organic matter in dry matter and metabolizable energy of alfalfa and alfalfa- sainfoin mixture. The results related to the digestibility according to the McNiven method showed that the addition of 1.5 and 3% of yarrow and ginger powder in sainfoin and alfalfa hay could reduce the amount of gas production at times 3, 6, 12, 24, 48, 72, 96 and 120 hours of incubation. However, the addition of yarrow and ginger powder to alfalfa- sainfoin mixture did not have a significant effect on the amount of gas produced. The results of the gas test method showed that the addition of 1.5% and 3% of yarrow and ginger in sainfoin and alfalfa hay reduced the digestibility of organic matter, total short chain fatty acids and metabolizable energy compared to the control group. Among these, 3% of ginger has the most significant reducing effect on the digestibility of organic matter from 67.80 to 50.14 and 45.99% (P=0.0092), total short chain fatty acids from 1.77 to 0.76 and 0.66 milli moles (p=0.0183) and metabolizable energy from 9.88 to 7.47 and 6.79 MJ kg-1 DM (P=0.0167) respectively in sainfoin hay. Also, this similar decreasing trend continued in alfalfa hay for the nutritional parameters of the gas test. A mixture of alfalfa and sainfoin was not affected by the addition of different levels of yarrow and ginger.

It is concluded that the addition of 1.5 and 3% levels of yarrow and ginger powder in sainfoin and alfalfa hay could not improve the amount of gas production.

Yarrow as a medicinal plant belongs to the family of Asteraceae that its' essential oils are used in health and pharmaceutical industries. In the present study, in order to evaluate and comparethe quantity and quality of essential oils of flowering shoots and individual plant parts (flowers, leaves and stems), Achillea wilhelmsii C. Koch plants were collected in flowering stage from the Qushchi in Urmia in West Azerbaijan province during June 2017. After drying the plant, the essential oils of the organs were carried out using Steam-distilled Clevenger. The Gas chromatography–mass spectrometry (GC/MS) analysis was used to analyze the essential oils. The results showed that the different organs differ in terms of percentage and essential oils composition. The highest oil yields were obtained from flowers (0.70%) and flowering shoots (0.62 %), leaves (0.50%), respectively and the lowest oil yield was obtained from stems (0.16%). Totally 22 essential compounds in flowering shoots, 12 essential compounds in flower, 9 essential compounds in leaves and 7 essential compounds in shoots were identified. The compounds of Camphor, Camphene, Borneol, 1.8-Cineole, Beta-Eudesmo, Alpha-Pinene and Caryophyllene oxide were the dominant components of the essential oils of organs. According to the results, the use of flowering branches are recommended for essential oil extraction due to the presence of higher percentages and more essential oil compounds.

The various medicinal functions of Yarrow have made it an important medicinal plant in medicine. Also, yarrow is a rich source of antioxidants and flavonoids that protect plants from the harmful effects of active oxygen species. The aim of this study was to investigate the effect of salicylic acid treatment on the expression pattern of two genes (aox2 and pal2) involved in the production of antioxidants and flavonoids in Yarrow plant. For this purpose, a factorial experiment was conducted in a completely randomized design including salicylic acid at two levels (0 and 50 mM) as the first factor and sampling time with two levels (24 and 48 hours) as the second factor in the greenhouse of the Faculty of Agriculture. First, the yarrow plant was subjected to hormonal treatment in Johnson's hydroponic environment, and 24 and 48 hours after the treatment, leaf tissue sampling was done. The results of qRT-PCR showed that salicylic acid stimulation significantly decreased the expression of two genes in the path of producing antioxidants in yarrow. The lowest relative expression of studied genes occurred 48 hours after treatment with salicylic acid. In general, the use of salicylic acid triggers molecular processes that result in the plant responding by changing the expression level of genes encoding antioxidants and flavonoids; therefore, it is suggested to use other stimulants such as jasmonic acid to investigate the expression of genes encoding antioxidants and flavonoids in future researches.

The aim of present research was to predict the kinetics of essential oil extraction during ohmic-assisted hydrodistillation by three different modeling (nonlinear regression techniques (mathematical), artificial neural networks (ANN), and fuzzy logic) techniques to compare the accuracy of those models. Based on the results obtained the ANN was the best technique among all implemented models in predicting of extraction yield. Four mathematical models (first order, second order, adsorption and sigmoid models) describing essential oil extraction has been fitted to the extraction yield experimental data. Results indicated that first order model could satisfactorily describe the extraction kinetics of essential oil with correlation coefficient (R2) equal 0.988 and root mean square error (RMSE) equal 0.00014. Neural network with one and two hidden layers and 4–30 neurons were randomly selected and network power was estimated for predicting the extraction yield. ANNs with Feedforward–backpropagation structure, Levenberg–Marquardt training algorithm and 3-11-11-1 topology deserved the maximum R2 (0.999) and minimum RMSE (0.0004). Fuzzy logic tool in MATLAB with Mamdani model in the form of If–Then rules along with triangular membership function was used for predict the extraction yield. Despite the fact that fuzzy logic warranted lower fitting rates (R2 = 0.997) than that of ANN, it was a powerful technique for fitting of extraction yield experimental data.

Here, the seeds of 10 different species of the Yarrow (Achillea spp.) originated from geographically different regions of Iran were planted in the greenhouse of medicinal garden of Hamedan (2017-2018), and subsequently phytochemical variation of the essential oils of each individual species collected before and after flowering were assessed using GS-MS. Before flowering, overall, 27 metabolites were detected, among which 1,8-cineol, artemesia alcohol, artemesia ketone, camphor, germacrene-D, linalool, Terpineol and nerolidol were the superior. The maximum amounts of 1,8-cineol was observed in A. alepica, A. eriophora, A. biberstinii, A. filipendula, and A. nobilis, while both A. alepica and A. eriophora contained the highest amounts of camphor. Meanwhile, here, the maximum amounts of Germacrene D were acquired in A. tenuifolia (43.19%) and A. wilhelmsii (47.03%). Upon flowering, overall, 78 metabolites were detected, among which Cubenol, Eucalyptol, Camphor, germacrene-D, sabinol, trans-Nerolidol, Chamazulene, Chamazulene, and 2-Cyclohexen-1-ol, 1-methyl-4-(1-methylethyl)-, cis- were the superior. Based on cluster analysis and PCA constructed by the metabolite data of before and after flowering, all the 10 species of the genus Achillea were respectively grouped into three and four distinguished categories. Lastly, the results indicated that both quantity and quality of chemical composition of yarrow essential oils could be influenced by species under study, various growth and development stages, genetic background and possibly geographical/climatological factors, and that there is an acceptable phytochemical variation among them.

Bacterial resistance is one of the problems in the world of medicine today. Many efforts are underway to find alternative methods, one of which is to use the capacity of native plants of each region. The chamber was powdered as a homogeneous mixture and its essential oil was extracted by water distillation. Then, the components of the essential oil were identified and the amount of the components was identified using a gas chromatography device connected to a mass spectrometer. GC-Mass analysis showed that the most important effective compounds with antimicrobial activity of this plant were 2- boronanone (12.36%), camphor (6.87%), 3-carn (5.35%). The range of growth inhibition zone varied from 9 to 40 mm. Essential oil with growth inhibition zone of 40 mm had the greatest effect against Staphylococcus aureus even in comparison with clindamycin, although essential oils had no effect on Pseudomonas aeruginosa and could not inhibit its growth. In general, essential oils had a better effect on gram-positive bacteria than gram-negative bacteria.

In this study diversity of 10 populations belong to Achillea biebersteinii Afan and Achillea wilhelmsii C. Koch in natural habits of West-Azerbaijan province including (Urmia-Nazlu, Oshnaviyeh-Shahraksanati, Naghadeh Mohammadshahsofla, Mahabad-Esakandi, Miandoab-Chalkhamaz, Simineh-Khorojishahr, Bukan-Aliabad, Mahmoodabad-Hosseinabad, Shahindezh-Sarojeh and Sardasht-Nalas) were evaluated. Essential oil composition of the aerial parts of plant was identified by (GC/MS) gas chromatography. Cluster analysis divided the populations into three distinct groups. In general 23 and 27 components were identified in the essential oil ofA. biebersteinii and A. wilhelmsii, respectively. The essential oil percentage of the A. biebersteinii was 0.56 to 1.10 and the essential oil percentage of the A. wilhelmsiiwas 0.49 to 0.68%. The population of Naghadeh belongs to A. biebersteinii had the highest essential oil percentage and the population of Bukan belongs to A. wilhelmsii had the highest amount of 1,8-cineole (72.88%), the population of Miandoab (A. wilhelmsii) was high in camphor (57.98%) and camphon (58.5%) and the Shahindezh population belongs to (A. wilhelmsii) had the high levels of piperiton (32%). Populations of Naghadeh belongs to (A. biebersteinii), Bukan (A. wilhelmsii), Miandoab (A. wilhelmsii) and Shahindezh (A. wilhelmsii) were superior in terms of performance and efficacy that can be used for the pharmaceutical industry and breeding programs.

The aim of this reserch was determining of the Achillea wilhelmsii C. Koch essential oils composiation that was growned in natural counditions.Collected plants were harvested at full bloom stage in july 2019 and dried in the shade of the room condition then pulverized in the form of a homogeneous mixture and its essential oil was extracted by distillation with water. The components of the essential oil were identified and measured using the gas chromatography device connected to the mass spectrometer. The results showed that the essential oil obtained from the dried vegetative body of the plant had a yellow color with a yield of 0.89%. The results of GC-MS showed that the essential oil of this plant in the desired area consisted of a combination of 106 substances. Percentage of total essential oil, the most important main compounds identified in essential oil were: α-Thujene (2.21%) Camphene (2.38%), Sabinene (0.53%), Eucalyptol(1,8-cineole) (5.71%), Terpinen-4-ol (2/55%), Nonanal (4/25%), Camphor (9/87%), (+) 2 Bornanone (5/59%), Endo-borneol (4/05%), α-Terpineo (2/46%), Rosefuran (3/52%) Caryophyllene (0.64%), α-Farnesen (1.83), Terpinolene (3.44%) and Caryophyllene oxide (5.62) -Based on our knowledge this is the first report of Zanjan Achillea wilhelmsii C. Koch.

Achillea is an important medicinal plant with many properties and applications which has distributed in different geographical regions of Iran. In this study, the characteristics of growth, essential oil percentage and ecological factors of four different Yarrow species included (A. setacea), (A. biebersteinii), (A. tenuifolia) and (A. wilhelmsii) were evaluated in order to better understanding the characteristics of different Yarrow species. Plant sampling was done at flowering time and 13 morphological traits of the species with ecological characteristics were evaluated. The essential oil was extracted from dried plants by using Klevenger's apparatus for four hours. Analysis of variance showed that there was variation among studied species in terms of traits studied. The species of A. setacea was superior and difference in terms of height, main stem circumference, leaf length, leaf width; the species of A. wilhelmsii was superior in terms of stem number, inflorescent number, leaf number. According to the cluster analysis the species were divided into two distinct groups. Correlation between traits showed that there was a significant correlation between some morphological traits and morphological traits with soil. Essential oil yields varied from 0.52 to 0.82%. Based on these results, the species of (A. setacea) and (A. wilhelmsii) were better in terms of growth and yield characteristics; also (A. wilhelmsii) was better in essential oils yield. Introduced plants of this research can be used in pharmaceutical, food and perfume industries and introduced for breeding programs.

The purpose of this study is to investigate the potential of some plants for Phytoremediation of soil contaminated with lead, zinc, cadmium and copper. To do this, four plant species of Achillea millefolium, Agropyron elongatum, Bouteloua dactyloides and Artemisia sieberi were cultivated and plant samples were irrigated with refinery wastewater. The results showed that in terms of plants, Bouteloua dactyloides, Artemisia sieberi and Achillea millefolium are good transfer of metals to their aerial parts which is suitable for plant extraction (the most important technique of Phytoremediation). Agropyron elongatum mostly accumulates copper and lead metals in the root. This is a proper feature of phytostabilization. Also the capability of these four plants species for Phytoremediation is as follows: Bouteloua dactyloides> of Achillea millefolium> Artemisia sieberi = Agropyron elongatum. Bouteloua dactyloides is proper for Phytoremediation of all heavy metals. For Bouteloua dactyloides, plant transfer factor (TF) in metals: zinc, copper, lead and cadmium is 1.17 and 1.9, 1.12 and 1.41, respectively, and bio concentration factor (BCF) for that in these metals are 1.77 and 1.22, 0.95 and 1.37, respectively. Since Bouteloua dactyloides has high tolerance to drought and high temperatures and is also suitable for lawn making, it is suggested that this type of species is used for Phytoremediation of soils contaminated with heavy metals in polluted areas as well as lawn making so that in addition to soil cleansing from heavy metals and being proper with native climates in many parts of Iran and having low irrigation requirements.

This experiment was conducted in a completely randomized design with three replications in 2016 in Laboratory of Agricultural College, Mohaghegh Ardebili University. Medicinal plants included Artemisia sieberi L., Achillea millefolium L., Salvia officinalis L., Matricaria chamomilla and control (distilled water). The results showed that the medicinal plants extract significantly reduced germination percentage, length and dry weight of Datura stramonium L. seedling compared to the control. Among the medicinal plants, extract of Artemisia sieberi L., and Salvia officinalis L, compared with other extracts, reduced germination percentage by 32% and 24%, respectively, compared to the control. In the extracts of Artemisia sieberi L., and Salvia officinalis L, dry weight of Datura stramonium L seedlings decreased compared to other plants extracts. In general, these herbs, especially Artemisia sieberi L. and Salvia officinalis, can be used in rotational programs to reduce the presence of weeds.

To study the effect of physical primings on growth and yield of Achillea santolina and A. millefolium two experiments were conducted in Islamic Azad University of Tabriz, Iran, with three replications during 2014. Treatments were ultrasonication, laser, magnetic field, gamma and beta radiations for 5, 10 and 15 min. along with a control. By increasing ultrasonic exposure time of Achillea santolina seed its germination was delayed. Seeds treated with ultrasonic wave for 5-10 min. and laser for 5 min. produced seedlings with higher vigors. Seeds of A. millefolium hydroprimed germinated only by 65%. Also, seeds of plants treated by magnetic field, gamma and beta radiations produced weaker seedlings. Achillea santolina plants treated by ultrasonic waves produced higher essence percentage. Essence yields of seeds of both species expesed to ultrasonic wave and laser were higher than the other ones. The effects of seedling vigor index and number of secondary branches on yield of both species were 1.90 and 1.85 times greater than that of final germination percentage. Ultrasonication and laser treatment of Achillea seeds resulted in both strong seedlings and higher essence yields.

The effect of salt stress on morphological and essential oil content of 10 Iranian and foreign Achillea millefolium genotypes was investigated in a pot experiment in Research Greenhouse of Isfahan University of Technology in 2014. The factorial experiment, based on randomized complete blocks design, was performed at four salinity stress levels (0, 5, 10 and 15 dS/m) andn ten Achillea millefolium genotypes with three replicates. Results revealed that salinity stress of 15 dS/m significantly decreased the plant height, leaf area and width, length of ligulate florets, flower diameter, number of florets in inflorescence, days to flowering, days to 100% flowering and dry weight of the herbs. The salinity stress at 15 dS/m increased essential oil content by 18.75% compared to control treatment. The UK, Slovenia, Spain, Japan, Kandovan (Iran) and Lorestan (Iran) genotypes were not able to produce flowers in high salt stress conditions. The interaction of salinity stress and genotype was significant for all traits except for leaf width and length of ligulate florets. The US and Canada genotypes possessed the highest essential oil content and dry matter weight. The US and UK genotypes can be suggested for green space, under salinity stress conditions, due to their low height and high flower diameter. Most of the studied traits did not show significant differences at 5 and 10 dS/m salinity stress levels. Plants had good resistnce to 10 dS/m salinity stress. Moreover, evaluated traits of plants were significantly different at 15 dS/m level. Based on the obtained results, the 10 dS/m salinity level can be suggested to cultivate this plant.

The aim of present study was to investigate the effects of A. millefolium hydroalcoholic extract on mucosal immune response (lysozyme, total immunoglobin and protease) as well as immune related (TNF-alfa) gene expression in Goldfish (C. auratus). For this purpose, 180 fish (average weight=0.192± 0.014 g) were randomly divided in 6 aquaria (two replicate) and were fed with A. millefolium extract (at three levels 0, 2 and 4 gr/kg) for six months. Evaluation of mucosal immune parameters revealed that supplementation of diet with A. millefolium significantly increased the lyzozyme and protease activity in fish fed with 4 gr/kg extract compared to control group and diet with 2 gr/kg. No significant differences were observed in total immunogloubin in fish fed with supplemented diet compared to control group). TNF-alfa gene expression data significantly increased in fish fed with A. millefolium extract compared to control group in a dose-dependent manner. According to the results obtained from this study, diet supplemented with A. millefolium extract could modulate skin mucus immunity as well as expression of inflammatory cytokine in goldfish.

Weeds pose a serious threat to farmers. Using allopathic properties of the plants, especially those of the medicinal plants, has an important role in controlling weeds. This study was conducted to examine the allelopathic potential of Achillea wilhelmsii in germination of Hirshfeldia incana and Hordeum glaucum. A factorial experiment was conducted, adopting a completely randomized design with three replicates in both libratory and pot cultivation. Organ extracts (flower, leaf and root) of Achillea wilhelmsii in four concentrations were used, namely: 1, 2 and 5%. In addition, distilled water was used as a control. The result showed that the greatest percentages of the germination of Hordeum glaucum and Hirshfeldia incana were in the distilled water, which were 100 and 91%, respectively and that their germination reached zero percent under extraction of leaf and flower with a concentration of 5%. The concentration higher than 2% of the extraction of Achillea wilhelmsii significantly decreased germination, length of radicle and aerial part, fresh and dry weight of shoot and root, leaf area index and amount of chlorophyll in Hordeum glaucum and Hirshfeldia incana, in contrast with distilled water. The results showed that leaf and flower extraction of Achillea wilhelmsii had allopathic effects. In addition, it had impacts on germination and growth of the seeds of Hordeum glaucum and Hirshfeldia incana, which could be used in the production of natural herbicides.
Highlights: 1. Aqueous extrac of Achillea wilhelmsii significantly decreases germination and growth of Hordeum glaucum and Hirshfeldia incana.
2.Aqueous extrac of Achillea wilhelmsii can be used to produce the natural herbicides.

This study was conducted to compare three herbal extracts (thyme, yarrow and garlic), antibiotic, and organic acids to reduce Campylobacter jejuni colonization in digestive tract of broiler chickens. A total of 336 one-day old male broilers (Cobb 500) were randomly allocated to 7 treatments, 4 replicates with 12 birds in each replicate with a completely randomized design. The experimental groups included negative control (basal diet without challenge), the positive control (basal diet with challenge), organic acids (1 mL per liter in drinking water for first 14 days of age, then 8 hours each day until end of the experiment), antibiotic (oxytetracycline 100 ppm in feed), yarrow extract (1 mL per liter in drinking water), thyme extract (1 mL per liter in drinking water), and garlic extract (1 mL per liter in drinking water) throughout the experiment. Except for the negative control group, all chickens were orally challenged with (109cfu/mL) C. jejuni suspension on day 21. The negative control group was inoculated with 1 mL of saline solution. Feed intake, weight gain, and feed conversion ratio were measured weekly. At end of the experiment (day 42), the blood samples were taken from the wing vein to determine serum biochemical parameters. The effect of treatments on the morphology of the small intestine, lymphatic organs weight, and colonization of C. jejuni were measured. The results of statistical analysis showed that treatments had significant effect (P>0.05) on Campylobacter jejuni colonization,so that, the highest and lowest levels of the bacterial colony were observed in positive control and antibiotic treatment. The negative control and yarrow treatments had a significant difference (P0.05). According to the results of this experiment, the use of above mentioned treatments had positive effects on decreasing serum cholesterol and triglyceride, as well as reducing the colonization of Campylobacter jejuni in digestive tract of broiler chickens.

RNA extraction is considered a crucial step in the molecular studies and the results of subsequent work such as, PCR, Real-TIME PCR and RACE PCR depend on the quality and quantity of the extracted RNA. Some standard methods are available in total RNA extraction of plant tissue, which are effective for the ordinary plants. But it is difficult to extract the total RNA from the plants with high levels of carbohydrates and secondary metabolites such as phenolic compounds. Yarrow is one of the valuable medicinal and industrial species of the pasture of Iran that contains high levels of secondary metabolites. So far, there is no report on a suitable method for extraction of total RNA from it.
Material and Total RNA extraction was done through three methods including RNXTM(-Plus), Lithium Chloride and Phenol-Chloroform. Then the quality and quantity of extraction RNA was evaluated.
The results showed that among the three methods, extracted RNA with phenol-chloroform had high quantity and quality as the A 260/280 ratio was well within the accepted range of 1.8-2.
Using phenol-chloroform method tends to remove polyphenols and polysaccharides from plant tissue cell contents and causes more efficient RNA extraction. Given of this, the phenol-chloroform method is recommended for total RNA extraction of valuable plant Yarrow in future studies.

Yarrow (Achillea millefolium) is an herbaceous and perennial plant species which belongs to the Asteraceae family. Yarrow's essential oil has different compounds of monoterpene and sesquiterpens, which its main constituents are pinene and linalool. These compunds have anti-microbial and anti-pest activities and also can be used in the food industry, perfumery and cosmetics.The aim of the present study was to use the degenerate primers approach in order to isolate Pinene synthase and linalool synthase genes from Yarrow plant. Up to date, there is no any report on the availability of these genes in the world gene bank. In this investigation the total RNA was extracted from Yarrow then pinene synthase and linalool synthase genes were isolated, using degenerate primers and Polymerase Chain Reaction (PCR). PCR amplified two bands of 250 bp and 720 bp. The sequence data were compared with NCBI gene bank data. The results of diversity study among varieties and families based on Pis and Lis genes showed most similarity between Achillea and Artemisia plants. Also this similarity was seen between Asteraceae and Lamiaceae families and these families grouped together same group. These results also showed a relatively high similarity of Pis and Lis with some other plants which confirmed sequencing data.