جستجوی مقالات مرتبط با کلیدواژه « Sperm quality » در نشریات گروه « کشاورزی »

Introduction : 

 Oxidative stress is an imbalance between oxidants and antioxidants at the cellular level which leads to the condition of infertility in male. Oxidation of cell macromolecules, cell death by necrosis, apoptosis and damage of tissue structure are the results of another oxidative stress damage. This process eventually leads to a variety of diseases, reduced growth performance and even death (Min et al. 2018). Poultry face a variety of environmental, technological, nutritional, and biological stresses that reduce their productivity and reproductive performance. Most of these stresses at the molecular level are associated with oxidative stress and damage to biologically important molecules (Surai et al. 2019). Qualitative characteristics of sperm, blood testosterone level, and plasma lipid peroxidation are affected by severe oxidative stress (Khan, 2011). Vitamin E is an important known antioxidant and protect cell membrane of sperm cell from damage of reactive oxygen species in male reproductive system of animal. Feeding of this vitamin has beneficial effect on testes weight, semen quality indexes, testosterone and antioxidant enzymes such as glutathione peroxidase and superoxide dismutase in birds and mammals. The objective of this experiment was to investigate the effect of diet vitamin E supplementation on fresh sperm quality in broiler breeder roosters challenged with oxidative-stress.

Eighteen Ross 308 male broiler breeder at 28 weeks of age were randomly assigned into 3 experimental groups including control group, dexamethasone group (subcutaneous injection of 4 mg dexamethasone per kg body weight) and dexamethasone group receiving supplemented diet with of vitamin E (200 mg per kg of feed). Each experimental group consisted of 6 birds. Sperm samples were taken from roosters using abdominal massage method and analyzed for quality characteristics and antioxidant status. Sperm concentration was determined by counting spermatpzoa using hemocytometer. The pooled sperm sample was diluted by using poultry semen extender and then 10 µl of diluted sperm mixed with 10 ml of 3% NaCl. The chambers of hemacytometer filled with 10 µl sperm suspension and allowed to settle for 3 mines.  Numbers of sperm cell in 5 of the large squares of chamber were assessed. Computer assisted semen analyses (CASA) were performed to determine sperm motility, with settings adjusted to detecting domestic fowl according to a previously described method (Froman and Feltmann, 2000). Total, progressive and non-progressive motility (%), as well as immotile sperms were measured. Also, sperm kinematic values including straight line velocity (VSL (µm/s)), curvilinear velocity (VCL (µm/s), average path velocity (VAP (µm/s), amplitude of lateral head displacement (ALH (µm), and beat-cross frequency (BCF (Hz) were measured. Progression ratios were also calculated using the mentioned velocity measurements: straightness (STR = VSL/VAP) and linearity (LIN = VSL/VCL).

The results indicate that dexamethasone-induced oxidant stress caused an increase in MDA levels (5.4) and a decrease in the activity of antioxidant enzymes such as glutathione peroxidase (52.51) and superoxide dismutase (108.62), as well as sperm motility parameters and sperm cell membrane integrity in comparison to the control group. However, when vitamin E was added to the roosters' diet, it improved the negative effects of oxidative stress on sperm motility parameters, antioxidant status (Total antioxidant capacity (1.65), superoxide dismutase (155.10) and glutathione peroxidase (87.77) enzymes), sperm viability (88.05), and sperm cell membrane integrity (89.72) (P<0.05). These findings suggest that dexamethasone injection caused a sharp decline in sperm quality by reducing sperm motility, antioxidant enzyme activity, and plasma membrane integrity, while vitamin E supplementation improved sperm quality by enhancing the antioxidant status and protecting the sperm cell membrane from the damage caused by reactive oxygen species in broiler breeders. Avian sperm are highly susceptible to lipid peroxidation due to their high content of long-chain polyunsaturated fatty acids. Lipid peroxidation produces reactive species that damage sperm membrane function and motility, ultimately reducing the fertility potential of aged roosters. Therefore, the antioxidant defense plays a critical role in maintaining semen quality (Surai et al., 2006).

It can be concluded that the inclusion of vitamin E in diet remarkably improved the sperm motility characteristics and antioxidant status of sperm in broiler breeder roosters challenged with oxidative stress. On bases of this finding it’s recommended to use of vitamin E in the rooster diet to elucidate negative effect of oxidative stress.

After being released into the aquatic environment, fish sperm have a limited time to reach the egg, two limiting factors for successful fertilization are the time it takes for the sperm to reach the egg and the other limiting time for the micropile hole to close. Sperm quality is one of the important factors in reproduction, which itself is influenced by various parameters such as motility time, sperm rate, osmolarity, sperm concentration in semen and ATP. In addition, nutritional parameters such as vitamins, minerals and fatty acids are involved in increasing sperm quality. It is very important to study the parameters and factors affecting sperm quality, because reproduction and reproduction of fish is one of the important production links in aquaculture. Also, studies on marine fish are much less than freshwater fish, and research in this area is needed for sustainable aquaculture.

The objective of the present experiment was the study of selenium nanoparticle (NanoSe) in freezing extender on quality parameters of frozen-thawed sperm in broiler breeder roosters reared under physiological stress. Semen samples were collected from 12 birds Ross308, 28 week-age-old, which randomly allocated 2 groups included with physiologic stress (DEX group: using the injection of dexamethasone 0.4 mg/Kg body weight, three times, every other day for 1 week) or without dexamethasone injection (CON). Subsequently, after preliminary evaluation, samples collected from CON and DEX groups were divided into 4 experimental treatments for freezing using modified Beltsville extender either with or without NanoSe. Experimental treatments were included: 1) CON: control group, 2) DEX semen sample from dexamethasone group, 3) CONNSe: inclusion of NanoSe in CON semen sample, and 4) DEXNSe: inclusion of NanoSe in the DEX semen sample. Results show inclusion of Nano selenium in extender of CONNSe improved the Total motility (TM), Progressive Motility (PM), Average Path Velocity (VAP), Straight Line Velocity (VSL), Curvi Linear Velocity (VCL), Linearity (LIN), Straightness (STR), Lateral Head Displacement (ALH) and Beat Cross Frequency (BCF) in comparison with other experimental groups (P<0.05) Also, the Viability and Integrity of sperm Membrane, Glutathione Peroxidase (GPX) and Superoxide Dismutase (SOD) activities were higher in mentioned groups then other experimental groups (P<0.05) but there were no significant differences among groups in Malondialdehyde (MDA) and Total Antioxidant Capacity (TAC) (P>0.05). While the inclusion of NanoSe had no positive effects in DEXNSe versus DEX group (P>0.05). It seems the inclusion of NanoSe in extender improves the quality of frozen-thawed sperm, while cannot compensate for the negative effects of oxidative stress.

Determination of the sub-lethal effects of diazinon toxin carried out on steroidogenesis and quality of sexual production in male goldfish breeders. For this purpose, fish were first exposed to different lethal concentrations of diazinon toxin (9, 10, 11, 12.5, 15 and 20 mg/L) and LC50 values were analyzed for 96 h using probit software.  The amount of LC50 was 11.4 in 96 hours and fish were assorted according to 0, 0.25, 0.50 and 0.75 percent of LC50 in 96 hours and were put there for 2 months. At the end of the experiment, the level of sex hormones and the quality of sexual production were studied in male broodstock. The results showed that diazinon toxin significantly reduced testosterone and 17-β-estradiol levels in the 0.50 and 0.75 mg/L treatments compared to the control (p<0.05). There was no significant difference in some sperm quality parameters such as sperm volume, sperm density and spermatocrit between the control and 0.25 treatment. By increasing the concentration of diazinon toxin (from 0.25 to 0.75 %), the duration of sperm motility decreased significantly. The percentage of motile sperm in the control treatment was higher than other treatment and it was decreased with increasing diazinon toxin concentration. The Gonadosomatic Index in the control treatment was significantly higher than the other treatments (p<0.05) and showed a decreasing trend with increasing toxicity. In conclusion, diazinon toxin in the aquatic environment, even at sub-lethal concentrations, causes undesirable changes in the sex steroid indices and quality of male gonads in goldfish.

The use of artificial insemination in dairy cattle industry may improve the results of selection for production traits. This study attempts to evaluated effect of GDF9A625T and GDF9A489T candidate gene polymorphism on the sperm quality and quantity traits in Iranian bulls. A total of 108 samples of blood and semen was collected of A. I. bulls were born between 1989-2016 years. The GDF9A625T and GDF9A489T SNPs was determined by PCR- RFLP with DraI and NsiI enzymes, respectively. In A625T site, A (351 and 25 bp) and T (376 bp) alleles and in A489T site, A (184 and 24 bp) and T (208bp) alleles was obtained and also three AA, AT and TT genotypes in two marker sites were observed. The frequency results of GDF9A625T site showed that the T allele relative to the A allele was more frequent in Iranian Holstein bulls (0.6343 vs 0.3657) and 44.44, 37.98 and 17.95 percent of bulls had TT, AT and AA genotypes, respectively. In GDF9A625T site, the T allele frequency was more than A allele (0.602 vs 0.398) and 40.82, 38.78 and 20.14 percent of bulls had TT, AT and AA genotypes, respectively. Analysis of variance revealed that the effects of both loci were significant effect on sperm quality in Iranian Holstein bulls. The result of means comparison between genotypes in two marker sites showed that bulls with AT genotype significantly high value in quantitative trait and AA genotype was significantly higher in qualitative trait. Results of this research showed that these marker sites can be beneficial in genomic selection.

The Effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°C was evaluated. Semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body weights of 64 kg. Semen samples were pooled and divided into 4 parts. Each part was diluted with one of the tris base extender containing 0.5, 1 and 1.5 percent of soybean lecithin and 14 percent of egg yolk. The motility, viability, morphological abnormalities and membrane integrity of spermatozoa and pH of semen were evaluated. The results showed that 1.5 percent of soybean lecithin improved the motility and viability of spermatozoa at the first time (P

In this study, nine Taleshi rams (approximately 3 years old and average BW was 55.6±1.57 kg) were selected randomly and allocated into three groups to investigate the effect of melatonin (regulin) on sperm during breeding and non-breeding seasons. The first group (control) did not receive any regulin, the rams of second group received one regulin and finally, the third group received two regulins subcutaneously at the same time. Scrotal circumference (SC), semen volume, sperm concentration, sperm motility and sperm viability of all rams were measured on day zero (regulin implantation day). Scrotal circumference was measured 45 days after regulin implantation and semen volume, sperm concentration, sperm motility and viability were measured 46, 50, 54 and 58 days after regulin implantation. The results indicated that the use of regulin had not any significant effect on the SC and sperm quantity and quality parameters during breeding season (P 0.05). The SC and motility were increased by using two regulins during non-breeding season and this improved sperm viability in comparison to control group (P<0.05). Whereas, the use of regulin had not significant effect on semen volume and sperm concentration (P>0.05). It is concluded that improvement in the ram semen quality was expected with the use of two regulins during non-breeding season.

The objective of this study was to investigate the effect of different levels of egg yolk on quality of Zel ram spermatozoa in pre and post freezing conditions. Semen samples were collected from 4 healthy and mature rams using an electro-ejaculator and suitable samples were mixed with Tris extender in a ratio of 1:4 semen and samples were assessed, after cooled to 5-0C. Then, 0.5 ml of straws were filled with diluted semen. At first, straws were frozen on liquid nitrogen vapor, then kept in liquid nitrogen and after 10 days straws were thawed to investigation of morphological characteristics, viability, motility and progressive motility of spermatozoa. This experiment was carried out on the basis of completely randomized design with 3 treatments include levels of egg yolk 10, 15 and 20% and 9 replications. Results showed that the effect of egg yolk on viability, motility, progressive motility, normal spermatozoa and recovery percentage of spermatozoa were not significant (P>0.05), but the higher quality of spermatozoa and supply of energy source given in 20% egg yolk for lifespan, despite the absence of significant, if price of egg yolk is economical, 20% egg yolk can be used in Tris extender for maintaining and long term storage of Zel ram spermatozoa.

The purpose of this study was to determine the effects of different concentrations of vitamin E (Vit. E) in the quality of preserved stallion semen at 5ᵒC for a period of 48 h. Semen was collected and most (≥90%) seminal plasma removed by centrifugation at 600 × g. Pooled samples divided in four equal parts and diluted with extenders that was supplemented with different concentrations of Vit. E [(0 (control، C)، 5 and 10 mM)] and DMSO positive control group [as vitamin E solvent، (DC)] to a final concentration of 50 × 106 sperm/ml. Motility، viability، plasma membrane integrity، total abnormality and lipid peroxidation were determined at 0، 6، 24 and 48h. The results showed that 5mM vitamin E resulted in higher viability and plasma membrane integrity compared to control after 48 h of storage (P<0. 05). Different concentrations of vitamin E had no significant effects on sperm motility and total abnormality compared to C and DC extender (P>0. 05). Also، 5mM Vit. E was decreased lipid peroxidation compared C and DC extenders after 48h of storage (P<0. 05). In conclusion، between above-mentioned different concentrations، 5mM Vit. E could improve Caspian stallion sperm quality during cooled-storage at 5°C.

This experiment was conducted to study the effect of induced transient hypothyroidism on pubertal initiation, histological traits of testis and semen quality in breeder roosters. A total of sixty-six broiler breeder male chicks were randomly assigned to two experimental groups of control or hypothyroidism in a completly randomized design with three pens per group. In hypothyroidism group, chicks were fed dietary treatment of 0.1% propylthiouracil from 6 to 12 week of age. Body weight, thyroxin and testosterone concentrations were measured at 3-week intervals from 6 to 18 and also at 26 week of age. Histological caracteristics and the expression of connecxin 43 gene in testis were assayed in experimental groups and semen quality was evaluated for 10 weeks after photostimulation. Connexin 43 gene expression was higher in control group (1.181) than hypothyroidism one (0.782) at 18 week of age (P<0.05) and was similar at 26 week of age (P>0.05). Semen volume was greater in hypothyroidism group (0.46 ml) than that of control one (0.39 ml). Also, sertoli cell number was greater in hypothyroid group at 18 (159.9 /mm2) and 26 (165.9 /mm2) week of age than that in control group (P 0.05). Also, relative testis weight was greater in hypothyroidism group (749 mg/100gBW) than control group at age 26 week of age (P<0.05). Therefore, addition of propylthiouracil in breeder male chicks diet in prepubertal period, probably resulted in higher sertoli cell number and testicular weight via delay in puberty.