جستجوی مقالات مرتبط با کلیدواژه "آزمون صفحه داغ" در نشریات گروه "پزشکی"

Artemisia persica is an indigenous plant of Iran that has been traditionally used to relieve neurological and visceral pain. The aim of this study was to evaluate the analgesic effects of Artemisia persica essential oil on pain induced by hot plate and formalin in mice. In this experimental study, male Balb/c mice were divided into 5 groups including normal saline, Artemisia persica essential oil (50, 75 and 100 mg/kg) and morphine (10 mg/kg). The hot plate test was performed at, 15, 30, 45 and 60 minutes after the IP injection of drugs. In the formalin test, 30 minutes after the IP injection of drugs, 20 ml of 4% formalin was injected in the animal's paw and the frequency of paws licking, clopping and lifting were recorded at 5 and after injection (acute phase) and 15-30 (chronic phase) minutes. Data were analyzed by SPSS16 software. Injection of Artemisia persica essential oil (75 and 100 mg/kg), 15, 30, 45 and 60 minutes before the hot plate test, significantly decreased pain threshold compared to the control group (p< 0.05) Artemisia persica essential oil at a dose of 100 mg/kg significantly decreased the frequency of paws licking, clopping and lifting at the first and second phase of formalin test (p<0.05). The results of this study indicate the effectiveness of Artemisia persica Boiss essential oil in relieving pain caused by chemical and thermal stimuli.

Food deprivation increases the expression of orexin-1 receptor gene in the hypothalamus. On the other hand, food deprivation induces analgesia and orexin has a fundamental and well known role in pain modulation. This study aimed to determine the role of orexin receptor 1 antagonist in analgesic effect of acute food deprivation (12 h) by hot plate test in male rats.
Male Wistar rats were divided into 5 groups (n=8), control, food deprivation without surgery, stereotaxic surgery with vehicle, vehicle and food deprivation and food deprivation and orexin receptor 1 antagonist groups. 12 hours before the hot plate test, food was removed from the animals’ proximity, but water was freely available. All groups were tested using the hot plate. Data were analyzed statistically by one-way ANOVA with Tukey post hoc test.
According to our findings, orexin receptor 1 antagonist injection increased pain related behaviors at the time of 5(P Orexin plays an important role in feeding state–induced pain modulation. Based on our results, change in pain behavior induced by food deprivation may be related to change in orexin expression or in orexin receptors density.

Pain is a warning factor that appears either acute or chronic in possible dangers. The usage of herbal plants instead of synthetic drugs has been increased in recent years due to slight complications and variety of effective components. The aim of this study is to investigate the effect of hydroalcoholic extract of Scrophularia striata Boiss. on the pain induced by hot plate test.
In this study, 40 male mice (approximate weight of 25-30 g) were randomly selected and divided into five groups of 8 included a control group (receiving normal saline) and 4 treatment groups (receiving the extract doses of 150, 300, 600, 900 mg/kg). After 30 minutes of Intraperitoneal injection of different doses of extract in treatment groups and normal saline as solvent in control group, mice were immediately transferred to a special cage and the hot plate test was performed at 15, 30, 60, 90, 120, 180 minutes.
Hydroalcoholic extract of Scrophularia striata Boiss. Cause short-time analgesia in hot plate test as acute pain evaluation model in mice. As time elapsed, compared to the control group, no significant difference in the analgesic effect was observed.
Hydroalcoholic extract of Scrophularia striata Boiss has a strong and appropriate antinociceptive effect and can be a good alternative to analgesic drugs. It is recommended to use other pain and inflammation tests and different fractions of extract.

Background and Ducrosiaanethifolia is an aromatic medicinal plant native to Iran, and has been used in traditional medicine for controlling infection, reducing anxiety and pain. Since analgesic effect of this plant has not been studied experimentally, the aim of the present research is investigating the analgesic effect of Dc. essential oil. In this experimental study, 48 adult Wistar male rats were examined. Rats were divided randomly into 6 groups (n= 8) including: control group, morphine group and Dc. essential oil group (0.06, 0.125, 0.25 and 0.5 ml/kg, IP). Antinociceptive effects of drugs were assessed using hot plate apparatus. The results were analyzed using SPSS using one-way analysis of variance (ANOVA) followed by post hoc Tukey. Differences were considered significant at p< 0.05. The Dc. essential oil (0.5 ml/kg) significantly decreased sensitivity to painin comparison with control group. Latency to onset of pain significantly increasedby the Dc. essential oil (0.125 ml/kg) 60 and 120 minutes after injection compared with control group. Also, the Dc. essential oil (0.25ml/kg) reduced pain 120 minutes after injection in comparison with control group. Based on the findings of present study, the Dc. essential oil has analgesic properties and this plant can lead to decreased sensitivity to pain at some doses in the hot plate model of pain.

The application of herbal plants instead of synthetic drugs is increasing in recent years because of their lower side-effects and high varieties of efficient components. The aim of the present study was to evaluate the antinociceptive effects of hydroalcholic extract of grape seed using hot plate and formalin test. This experimental study has been done on 56 NMRI male mice (28±3 g) (n = 7 in each group) in weight. The hot plate and formalin test were used for acute and chronic pain. After preparation of extract، to evaluate the effect of pain related behaviors on antinociception، animals systemically received grape seed extract (75، 150، 300، mg/kg) and after 30 min pain related behaviour was monitored in formalin and for hotplate tests. The data was analyzed using SPSS software and ANOVA at significance level p < 0/05. The results showed that hydroalcholic Grape seed extract (75، 150، 300، mg/kg)، significantly can induce antinociception in both phases of formalin test. Also، the Grape seed extract caused delay in painful behaviours (licking and jumping) in hot-plate test. The present results show that hydroalcoholic extract of grape seed has analgesic properties in response to acute and chronic pain in the formalin with hot plate tests and can be substituted for chemical analgesic drugs.

The use of medicinal plants due to their lower side effects and the various efficient components has been increased in recent years. The aim of this study was to evaluate the antinociceptive effect of hydroalcoholic extract of green tea in male mice using the hot plate and formalin tests. In this experimental study، 56 NMRI male mice (weight، 28±3 g) were assigned to the control and experimental groups. The experimental group received the green tea extract (75، 150 and 300 mg/kg) and 30 min after the injection، the hot plate and formalin tests were used to assess the acute and chronic pain. Results showed that hydroalcoholic extract of green tea (75mg/kg) could induce antinociception in phase 2، not in phase 1 and interphase of the formalin test. Hydroalcoholic extract of green tea (150 and 300 mg/kg) could also induce antinociception in phase 1، interphase and phase 2 of the formalin test. Moreover، hydroalcoholic extract of green tea caused a delay on the painful behaviors in hot-plate test. Hydroalcoholic extract of green tea has analgesic properties and can be used as a substitute for chemical analgesic drugs.

Long-term consumption of many drugs followed by reduction of their effectiveness has necessitated performing research on new analgesics. Thus، the present study was conducted to evaluate the analgesic effects of mentha longifolia and morphine in mice using writhing and hot plate tests. In this experimental study، 70 male rats were divided into 7 equal groups. The groups included the control، three experimental groups receiving 400، 800، or 1600 mg/kg of mentha extract and three experimental groups which received 2، 4، or 8 mg/kg of morphine. In order to measure pain، the two acceptable tests، writhing and hot plate tests، were applied. Pain scores were measured at 0، 15، 30، 45 or 60 min after administration of algogenic stimulus. It was found that in hot plate test، only the dose of 1600mg/kg of Mentha extract after 60 minutes was significantly able to exert an analgesic effect (P<0. 05). In wrighting test، mentha extract at different doses significantly reduced the number and time of wrightes in the rats، comparable to morphine (P<0. 05). It seems that all doses of mentha extract in wrighting test have analgesic effects which indicate chronic pain inhibition of mentha hydroalcholic extract.

Some of medicinal plants have been used for pain reliving in Iarnian ancient medicine. Astragalus gummifer (AG) is one of them and its gum (tragacanth gum) were used for several health purposes. The present study was conducted to evaluate the analgesic effects of this gum in mice. In this study, the analgesic effect of tragacanth gum was determined using hot-plate and writhing tests. Mice were injected with tragacanth gum at doses of 125, 250 or 500 µg/kg i.p. as treatment groups. Morphine at doses of 2, 4 or 8 µg/kg i.p., and diclofenac at doses of 10, 20 or 30 µg/kg i.p. were used as control groups. In writhing test, gum tragacanth at different doses (125, 250, and 500 µg/kg) significantly reduced the number of writhings in mice as compared to control group (p<0.05). In hot-plate test, maximum possible effect of tragacanth gum significantly increased only after 15 minutes but not in other time periods. In two models of pain assesment, both morphine sulfate and diclofenac sodium had also pain relieving potential. Coclusion: The present study indicated that tragacanth gum elicits prominent analgesic effects in an experimental model of acute and chronic pain. Additionally, data obtained in this study indicated the presence of some constituents with pain releiving properties that confirms the traditional use of the gum for pain relieving purposes.

The u se of medicinal plants to reduce pain is important. Tanacetum parthenium has been introduced as an analgesic agent in traditional medicine and is widely used to relieve neuropathic pain and headache.

The aim of this study was to investigate the analgesic effect of Tanacetum parthenium ethanolic extract on acute pain.

This study was carried out on 100 male mice weighing 30-35g. Acute pain was investigated using a hot plate test with set point 48 °C and cut off time of 30 seconds. In this experiment 100 mice were divided into 10 groups as follows: 1) control group; groups 2-6 received 10, 20, 30, 40 and 80 mg/kg of alcoholic extract, respectively; group 7 received 100 mg / kg ibuprofen; group 8 received 0.5 mg / kg morphine; group 9 received 0.5 mg / kg naloxone; and finally group 10 received naloxone and extract. Ethanolic extract of aerial parts was prepared by maceration method and later its analgesic effect was studied at different doses of 10, 20, 30, 40, and 80 mg/kg, i.p. The effect of ethanolic extract and fractions were compared with the analgesic effect of morphine and ibuprofen as standard analgesic drugs. Naloxone was used to study the opioid system. Data were analyzed by SPSS using Kruskal Wallis test.

Results obtained from this study showed that the ethanolic extract of Tanacetum parthenium produced an analgesic effect (P<0.05) at two doses of 30 and 40 mg/kg, i.p. The analgesic effect of extract was not lower than that of morphine (10 mg/kg, i.p.), and ibuprofen (100 mg / kg) (P<0.05). Application of naloxone showed no inhibition on analgesic effect of the extract (P<0.05).

The analgesic effect of Tanacetum parthenium was also comparable to that of morphine and ibuprofen, both well known for their analgesic effects. Further investigations to establish a link between the analgesic effect of Tanacetum parthenium and particular phytochemicals, are recommended.

In this study, the analgesic effect of aqueous extract of spearmint, which has been used in traditional as an anaigesic anti-rheumatic, medicine was considered. 90 male rats, weighing 200-250 g, were in 10 groups. Aqueous extract of spearmint with different doses including. 0.9, 2.7, 6, 35 and 45 mg/Kg was injected divided. to the experimental animals. the positive control group received Aspirin with diffe the positive control group rent doses of 50, 100, and 300mg/Kg. Normal saline (1ml/Kg) was injected to the negative group. Hot plate method was used for evaluating The analgesic effect of the spearmint extracts and the drug were measured in periods of 15, 30, 45 and 60 minutes after injection. The results showed that the extract at a doses of 2.7 and 6 mg/Kg has a significant analgesic effect with the highest analgesic effect by the letter. In addition, the results indicated that the 6 mg/Kg of menthe spicata extract was more effective than aspirin 300mg/Kg in the posi tire group. It seems that dose of the aqueous extract of spearmint has morked analgesic effect and can be considered as a herbal medicine in pain treatment in humen decrease in neuron permeability of Ca++.