جستجوی مقالات مرتبط با کلیدواژه "اندومتریوز" در نشریات گروه "پزشکی"

Endometriosis affects approximately 10% of women and girls of reproductive age. The present study was designed and implemented with the aim of determining the relative frequency of endometriosis symptoms and related factors in women referring to health and treatment centers in Yasuj, Iran, in 2023.

In the present descriptive-analytical study conducted in 2023, the studied population were non-pregnant women of reproductive age of 15-50 years who were referred to health centers of Yasuj, Iran, and 865 of them were selected by convenient sampling method. The participants were interviewed with the self-assessment questionnaire included in the educational booklet of the Ministry of Health, Treatment and Medical Education, including 12 symptoms of endometriosis, in order to identify suspected cases of the disease, if they have five or more symptoms. The data was analyzed with SPSS version 27 statistical software and using descriptive statistics indicators such as mean, standard deviation, frequency distribution tables and inferential statistics of Chi square test.

The findings indicated that the highest frequency of endometriosis symptoms related to irregular menstrual periods in the last 6 months was 413 (47.7%) and the lowest was infertility in 91 (10.5%). In 209 (24.2%) they were suspected to have the disease and 94 (10.9%) had a history of endometriosis. The frequency of endometriosis symptoms in women aged 21-30, married, housewives, with a family history of endometriosis, history of using tobacco in the last 6 months, exposure to tobacco smoke, daily use of caffeinated substances were significantly higher(p<0.001).

In the present study, approximately a quarter of women of reproductive age needed to be referred to a specialist physician and follow-up due to the possibility of contracting this disease, which indicated the importance of screening women of reproductive age and timely diagnosis of this disease in order to prevent its complications and consequences, including infertility.

Endometriosis is a chronic gynecological disease that can lead to infertility. Considering that the uterus is not sterile, the reflux of menstrual blood to the pelvis in these patients may be contaminated with bacteria and bacterial endotoxin, contributing to the development of endometriosis lesions. This study was conducted with aim to investigate the relationship between endometriosis, infertility, and uterine bacteria in women with endometriosis and infertility.

This case-control study was conducted during summer to autumn of 2021 on the endometrial bacteria of 35 women with endometriosis and infertility, as well as 15 healthy women in Zeinabieh Hospital, Shiraz. Bacterial isolates were identified and analyzed using biochemical and molecular methods. Then, the chi-square test and SPSS (version 21) were utilized to explore the relationship between uterine microbiota and endometriosis and infertility.

The isolates obtained from the uterine endometrium of sick women included Enterococcus faecalis (31.8%), E. coli (18.2%), Streptococci pyogenes (6.8%), Klebsiella pneumonia (13.6%), Klebsiella aerogenes (9.1%), Staphylococci aureus (4.5%), Pseudomonas aeruginosa (9.1%), Staphylococci Epidermidis (6.8%) and in healthy women were Lactiplantibacillus plantarum (45%), E. coli (15%), Acinetobacter baumannii (10%), and Staphylococci epidermidis (30%). According to the results of chi-square test, there was a significant relationship between the type of bacteria (normal flora and pathogenic) and the studied groups (p<0.05). Among the bacteria isolated from sick and healthy women, E. faecalis (31.8%) and L. plantarum (45%) were found to be the dominant genera.

The reduction of Lactobacillus species and the increase in the diversity of pathogenic bacteria species in the uterus are associated with an increased susceptibility to endometriosis and infertility.

 Endometriosis (EMS) is a female reproductive system disease in which uterine-like cells grow in other areas of the body and outside the uterus. Matrix metalloproteinases (MMPs) play a major role in the degradation of the extracellular matrix and the pathogenesis of endometriosis. The present study is the first to investigate the possible association of MMP-2 (rs7201) and MMP-9 (rs17576) genetic variants with susceptibility to EMS in Iran.

This case-control study was performed on 100 healthy control women and 100 patients with EMS. The MMP-2 and MMP-9 genotypes were determined using the Tetra amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR) Technique. Data were analyzed in SPSS software.

There were statistically significant differences in the distribution of MMP-9 AG (OR: 4.11, 95% CI: 2.13-7.91, P<0.001) and GG (OR: 11.33, 95% CI: 4.23-30.3; P <0.001) genotypes between the control and patient groups. The MMP-9 G allele was associated with an increased risk of endometriosis (P<0.001). Moreover, a significant association was detected between MMP-2 rs7201 polymorphism and EMS. MMP-2 AC genotype had a protective effect on endometriosis (OR: 0.27. 95% CI: 0.15-49%; P<0.001), and the MMP-2 A allele was associated with decreased risk of endometriosis (P<0.001).

As evidenced by the results of this study, MMP-9 rs17576 and MMP-2 rs7201 polymorphisms were associated with EMS in the studied population in Iran. Further studies with larger samples of different ethnicities need to confirm these results

Endometriosis is a long-term health condition that is known as a significant factor in women's infertility issues nowadays. Since many factors contribute to the development of this disease and because diagnosis is difficult and typically happens late in the progression of the disease, early detection is especially crucial. Extracellular micro vesicles called exosomes have diverse biological molecules and can alter the behavior of recipient cells, influencing angiogenesis, fibrosis, and inflammation. Exosomes are significant in cell communication and could act as new biomarkers for early detection of endometriosis. Hence, exploring and comprehending the impact of exosomes on the development of endometriosis can create opportunities for studying potential diagnostic biomarkers and targeted therapeutic interventions to prevent the advancement of the condition. This article delves into new approaches to diagnose and treat endometrial disorders by highlighting the various functions of exosomes in endometriosis.

Among the important factors affecting female infertility, endometriosis (with a prevalence rate of up to 50% in infertile women) has a special place. Endometriosis, which is known as the presence of endometrial glands and stroma outside the uterine tissue, causes a wide range of functional disorders in the process of follicular development and changes in the follicular microenvironment, which ultimately leads to the creation of an egg that is of suitable quality for the formation of The fetus does not have Therefore, the aim of the present study was to determine and investigate post-translational changes of histones, DNA methylation level and ERβ protein level in the genome of cumulus cells of infertile women with endometriosis.

The present case-control study was conducted at the infertility treatment clinic of Royan Research Institute in 2014. Twenty-four patients were divided into two equal groups. Cumulus cells were obtained from 12 infertile patients with endometriosis and 12 women with male factor infertility (as a control group) under ovulation stimulation protocols for intracytoplasmic sperm injection. Extraction of chromatin from cumulus cells was done after stabilization of DNA binding proteins and then cell lysis and preparation of soluble chromatin. The binding and incorporation levels of MeCP2 protein (as DNA methylation marker), two epigenetic markers related to histone modifications (H3K9me2 and H3K9ac), and ERβ protein to chromatin of cumulus cells were evaluated using the corresponding primary antibodies, secondary antibodies conjugated and Nucleosome-ELISA technique. Data were analyzed using independent t-test t and Levene's test.

MeCP2 protein incorporation into DNA was significantly higher in the endometriosis group than in the control group. The level of two epigenetic marks H3K9ac and H3K9me2 in the chromatin of cumulus cells of the patient group showed a significant increase compared to the control group (P<0.05). In addition, an increased level of binding of ERβ protein to the genome was observed compared to the control group.

Epigenetic changes, including histone hyperacetylation and hypermethylation, and DNA hypermethylation of the whole genome of cumulus cells of patients with endometriosis had occurred, accompanied by an increase in the level of ERβ protein binding.

Endometriosis is a chronic and promising yet benign disease in women of reproductive age. Some genetic markers in endometrial have a key effect in the pathogenesis of endometriosis. MicroRNAs are small molecules involved in several biological processes. The aim of this study was to investigate the differential expression of microRNAs, including miRNA451, during the secretory phase in patients with endometriosis.

The present study was conducted as a case-control study. Twenty infertile women with endometriosis who were referred to the Center for Research on Reproductive Health and Infertility of Babol University of Medical Sciences and Fatima Al-Zahra Infertility Specialized Treatment Center in Babol and were diagnosed by sonography or laparoscopy or laparotomy and tissue histology and after exclusion of individuals with other causes of female infertility were included in this study. Also 20 healthy women who had no female factor infertility were selected as the control group. The relative expression levels of mir451 in the endometrium of women in both groups were identified using Real-time PCR.

The mean level of mir451 expression in the endometrium of the endometriosis group was 0.46306 ± 0.71740 and in the control group it was 2.11621 ± 3.32579, which was significantly lower in the endometriosis group (P < 0.001).

The results of this study showed a significant reduction in the expression of mir451 in the endometriosis group compared to the control group. This could be investigated as a potential causative factor or indicator of endometriosis. Therefore, with further studies, it may be possible to use these microRNAs as diagnostic biomarkers for patients with endometriosis.

The relationship between endometriosis and thyroid disorders and the subsequent impact on fertility outcomes is still controversial. The present study was performed with aim to evaluate the effect of impaired thyroid-stimulating hormone (TSH) levels on fertilization rate of infertile women with endometriosis.

In this secondary analysis of cohort data, 179 infertile women with endometriosis were selected as the case group and 200 women with diagnosis of tubal factor or unexplained infertility were selected as the control group. Two subgroups of TSH levels above 2.5 mlU/L (with or without levothyroxine use) and TSH levels less than 2.5 mlU/L with levothyroxine use as patients with thyroid dysfunction and women with TSH levels less than 2.5 mlU/L without use of Levothyroxine were considered as thyroid-healthy subjects. Antral follicle count and fertilization rate were compared between the two groups. Data analysis was done using SPSS statistical software (version 24) and ANOVA test. P<0.05 was considered significant.

The mean of TSH in women with endometriosis was significantly higher than the control group (P=0.02, Cohen d=0.77, 95% CI: 0.02-0.43). When women were classified based on the presence or absence of endometriosis and serum TSH levels above 2.5 mlU/L, serum levels of FSH (P=0.76), LH (P=0.91), and fertilization rate (P=0.51) did not significantly differ between the two groups.

It seems that thyroid disorders are more common in women with endometriosis. However, there was no significant effect on the results of assisted reproductive procedures related to the coexistence of endometriosis and higher TSH levels.

Disturbance in the immune system is one of the main causes of endometriosis. Previous studies have shown the role of physical activity and antioxidant supplements on regulating the expression of genes related to improving the immune system, and reducing inflammation and infection. The present study examines the effect of swimming and vitamin E supplementation on the expression of NFKβ1 and P65 genes in endometriosis model rats.

Thirty female rats were divided into six groups (n=5 per group); healthy, endometriosis, without exercise-saline-endometriosis, exercise-saline-endometriosis, without exercise supplement-endometriosis, and exercise-supplement-endometriosis. Endometriosis was surgically induced. Physical activity included swimming for eight weeks, five sessions/week and 30 minutes/session, and supplement was provided by daily gavage of 200 mg of vitamin E/kg for eight weeks. Twenty four hours after the last swimming session, the animals were sacrificed and the endometriosis tissue was removed. Relative expression levels of NFKB1 and P65 genes were measured using Real-Time PCR. Data were analyzed using one-way and two-way ANOVA.

Induction of endometriosis caused a significant increase in the expression of genes studied (P<0.05). Exercise and vitamin E independently caused a significant decrease in the genes expression in rats (P<0.05). No synergistic effect was observed regarding the effect of exercise and vitamin E on the dependent variables (P>0.05).

According to this study, swimming and vitamin E supplement can be considered in improving endometriosis.

Endometriosis is a chronic, inflammatory and estrogen-dependent disease characterized by the growth of endometrial tissue outside the uterine cavity. The responses of the immune system, nutrition, sports activity are among the most important factors affecting this disease. The research aimed to investigating the expression of TLR4 and MyD88 pathway after a period of exercise and vitamin E consumption in endometriosis model rats.

The research is of fundamental and experimental type. 25 female Wistar rats were selected and divided into 5 different groups. After induction of endometriosis, rats in the training group swam 5 days a week. The vitamin E consumption group consumed the supplement daily. The studied proteins included TLR4 and MyD88. One-way analysis of variance and Bonferroni's post hoc test were used at the significance level of P≤0.05.

There was a significant difference between the research groups. The results of Bonferroni post hoc test showed that the expression level of MyD88 gene in healthy control group differed from endometriosis model control groups (P=0.0001), exercise group (P=0.019) and patient control group+vitamin E supplement (P=0.021). Significantly, the control group of endometriosis model was significantly different from the exercise group (P=0.002), the patient control group+vitamin E supplement (P=0.002) and the patient group+vitamin E+exercise (P=0.0001). There was a significant difference in the expression of TLR4 gene in the healthy control group with the control group of endometriosis model (P=0.048).

Induction of endometriosis strongly affects this pathway to cause inflammation in the tissue. Exercise with enzymatic adaptations may help slow the pathway and prevent inflammation and tissue cell proliferation down.

Formation of a retroperitoneal abscess after endometriosis laparoscopy is a rare complication, but if occurs, it may cause morbidity and mortality. Hematoma at the trocar site, if not detected during surgery, may cause retroperitoneal abscess. In this case, we have investigated and managed a specific case of retroperitoneal abscess after endometriosis laparoscopy. A 38-year-old female patient was diagnosed with extensive endometriosis and underwent laparoscopy. Four days after surgery, she experienced swelling and pain at the suprapubic trocar site. On the 11th day after surgery, the patient was referred due to abundant purulent secretions from the suprapubic trocar site and hospitalized. In the CT scan, there was a large collection in the pelvic extraperitoneal space of the Retzius space and the interior of the bladder, spreading to the paracolic gutters and the subhepatic area. Intramuscular meropenem and vancomycin were prescribed for treatment. Drainage of the above collection was done under ultrasound guidance. Three-hundred milliliters of pus was drained and an 8 French pigtail drain was placed percutaneously in the suprapubic area. 48 hours after discharge, the patient's fever stopped. After 14 days, purulent discharge from the drain stopped and the drain was removed. It seems that the correct techniques of trocar placement along with a complete understanding of the anatomy of the anterior abdominal wall, as well as paying attention to the diagnosis of vascular damage at the site of the surgical trocar are effective in preventing hematoma at the laparoscopic port site and preventing subsequent potential complications.