جستجوی مقالات مرتبط با کلیدواژه "درمنه" در نشریات گروه "پزشکی"

Nosocomial infections are considered as the important parts of the treatment challenges in hospitals. The genus Artemisia is widely distributed in Iran. Their species produce antibacterial, antiviral, and antifungal compounds belonging to different groups including phenols, terpenoids, sterols and polyacetylenes. The purpose of the present study is to investigate the in vitro effects of different hydroethanolic extracts of Artemisia species against bacterial strains in nosocomial infection.

12 different extracts, including 50% and 70% hydroethanolic extracts were prepared from the aerial parts of Artemisia ciniformis, A. turanica, A. kopetdaghensis, A. khorasanica, A. vulgaris, and A. sieberi. The winterization of the extracts produced corresponding defatted extracts. The minimum inhibitory concentration (MIC) values of the extracts against the bacterial strains Staphylococcus aureus, Streptococcus pyogenes, Acinetobacter sp., Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococus epidermidis, Micrococcus luteus, Klebsiella  pneumoniae and Escherichia coli were measured using the microdilution broth method. Total phenolic contents were determined by the Folin-Ciocalteu method.

Among the extracts analyzed in this experiment, the lowest MIC value was observed for the 50% hydroethanolic extract of A. turanica (0.25 mg/ml) against S. epidermidis. The largest range of bacterial sensitivity (6 strains) was related to the 50% hydroethanolic extracts of A. turanica (defatted and non-defatted) and A. kopetdaghensis (defatted). The growth of S. epidermidis was inhibited by all of the extracts. The highest total phenolic content and yield of extraction were recorded for 70% hydroethanolic extract of A. sieberi and 50% hydroethanolic extract of A. ciniformis, respectively.

The 50% hydroethanolic extract of A. turanica was superior to the other extracts in terms of the in vitro antibacterial spectrum and selective potency. A. turanica and A. kopetdaghensis are probably suitable choices for further phytochemical and antibacterial investigations.

Dermatophytosis is a significant skin disease in humans and animals, with increasing resistance to conventional treatments. This study aimed to determine the antifungal activity of petroleum ether, dichloromethane, ethyl acetate, ethanol, and hydroethanol extracts from the aerial parts of Artemisia khorassanica, Artemisia scoparia, and Artemisia vulgaris against common dermatophytic fungi, including Trichophyton rubrum, Trichophyton verrucosum, Epidermophyton floccosum, and Microsporum canis.

This descriptive study examined the antifungal activity of various extracts from the aerial parts of Artemisia khorassanica, Artemisia scoparia, and Artemisia vulgaris against fungal isolates of Trichophyton rubrum, Trichophyton verrucosum, Microsporum canis, and Epidermophyton floccosum. The aerial parts of the three Artemisia species were extracted using five solvents: petroleum ether, dichloromethane, ethyl acetate, ethanol, and 50% hydroethanol. The extracts were screened for antidermatophytic effects against the fungi mentioned above, and the minimum inhibitory concentration (MIC) was determined using the agar dilution method. The most active extracts underwent preliminary phytochemical testing.

In the initial screening, Microsporum canis and Trichophyton verrucosum showed the highest sensitivity (86.66%) and resistance (100%) to the tested extracts, respectively. Extracts from Artemisia scoparia exhibited the broadest range of activity. The most effective extracts, with an MIC of 78.12 µg/ml, were also from this plant. Hydroethanol extracts from all plant species demonstrated the least antidermatophytic activity. Preliminary phytochemical studies revealed the presence of terpenoids in all petroleum ether and dichloromethane extracts of the plant species.

Some lipophilic compounds, particularly in the petroleum ether and dichloromethane extracts of Artemisia scoparia, exhibit significant in vitro antidermatophytic activity.

About 75% of women experience at least one vulvovaginal candidiasis during their life. Clotrimazole is an antifungal drug that has side effects along with its therapeutic properties. For this reason, a large percentage of women tend to use natural herbal treatments. Artemisia is an anti-fungal activity.

The aim of this study was to compare the effect of Artemisia vulgaris and Clotrimazole on vaginal candida infection on non pregnant women in fertility ages.

This study is a triple blind clinical trial performed on 72 non-pregnant women in Mashhad in 2016-2017 years. The research units were divided into two groups (receiving Artemisia Vaginal Cream and receiving Clotrimazole Vaginal Cream). After confirmation of candidacy and having consent for participation, by random allocation and treated for seven nights. After the treatment, the data was analyzed using SPSS software (version 24).

After treatment, the score for candidate vaginal symptoms was not significant in Artemisia and Clotrimazole group based on Mann-Whitney test (P = 0.518). The Wilcoxon test showed a significant reduction in the symptoms of vaginal candida infection before and after treatment in both groups of Artemisia (P <0.001) and clotrimazole (P <0.001).

Due to the similar effects of Artemisia and Cholitrimazole, Artemisia can be used as an alternative treatment for vaginal candidiasis due to less side effects.

Artemisia persica is an indigenous plant of Iran that has been traditionally used to relieve neurological and visceral pain. The aim of this study was to evaluate the analgesic effects of Artemisia persica essential oil on pain induced by hot plate and formalin in mice. In this experimental study, male Balb/c mice were divided into 5 groups including normal saline, Artemisia persica essential oil (50, 75 and 100 mg/kg) and morphine (10 mg/kg). The hot plate test was performed at, 15, 30, 45 and 60 minutes after the IP injection of drugs. In the formalin test, 30 minutes after the IP injection of drugs, 20 ml of 4% formalin was injected in the animal's paw and the frequency of paws licking, clopping and lifting were recorded at 5 and after injection (acute phase) and 15-30 (chronic phase) minutes. Data were analyzed by SPSS16 software. Injection of Artemisia persica essential oil (75 and 100 mg/kg), 15, 30, 45 and 60 minutes before the hot plate test, significantly decreased pain threshold compared to the control group (p< 0.05) Artemisia persica essential oil at a dose of 100 mg/kg significantly decreased the frequency of paws licking, clopping and lifting at the first and second phase of formalin test (p<0.05). The results of this study indicate the effectiveness of Artemisia persica Boiss essential oil in relieving pain caused by chemical and thermal stimuli.

Candida albicans (C. albicans) as an opportunistic pathogen, can causes cutaneous and mucosal infections. Antibiotic treatments for this fungus have multiple adverse effects and cause drug resistance. In this study, the inhibitory effect of Artemisia sieberi (A. sieberi), was investigated on growth of Candida albicans.
In this experimental study, aqueous and alcoholic extracts were prepared from A. sieberi using percolation method. Using disk diffusion method, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), were assessed to evaluate the antifungal effects of these extracts on C. albicans in vitro.
C. albicans, was resistant to hydroalcoholic extracts in the disk diffusion method and grew around the disks containing the extracts. The MIC of aqueous, ethanolic, and methanolic extracts, were determined to be 6250, 3125, and 6250µg/ml, respectively, and MFC of these three extracts, were determined 12500, 6250, and 12500µg/ml, respectively. In the MIC and MFC method, ethanolic extracts had higher antifungal activity.
According to the results of this study, different extracts of A. sieberi affect C. albicans growth and prevent its growth. Thus, more

Background and Hydatidosis is highly prevalent worldwide which mainly involves liver and lung. There are some drugs sensitive to the hydatid disease but surgery is still the most common form of treatment. Different chemical agents are used for inactivation of protoscolices during surgery but most leave serious side effects. Therefore, herbal extracts have received more attention as replacing agents that have acceptable scolicidal effects and no adverse effects. The aim of this study was to investigate the scolicidal effects of mixture of Artemisia, Eucalyptus and ginger extracts on hydatid cyst protoscolices in vitro.
Hydatid liver cysts isolated from sheep were collected from a slaughterhouse in Hamedan, Iran. The cysts fluid containing live protoscolices were aspirated aseptically. The effect of two concentrations of the methanolic extracts of the mixture (50 and 100 mg/ml) was investigated at 15 and 30 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Data analysis was done applying Chi-square test.
The mixture of eucalyptus and ginger extracts showed acceptable scolicidal effects in which after 15 and 30 min exposure at 50 and 100 mg/ml concentrations it killed 97.24% and 100% of protoscolices, respectively.
This study showed that mixture of Eucalyptus and ginger had high scolicidal activity in vitro condition. Therefore, it could be used in surgical treatment of hydatid cyst after complementary researches.

Hormonal disorders along with oxidative stress and inflammation in ovarian tissue lead to anovulation in polycystic ovary syndrome (PCOS) patients. Considering antioxidant effect of Artemisia absinthium, purpose of this study is to determine the effect of aqueous extract of Artemisia absinthium on serum level of gonadotropin, estradiol, testosterone, inflammatory cytokines and oxidative stress indices of ovarian tissue in polycystic ovary syndrome rat model.
In this experimental study, 32 female Wistar rats were divided into 4 equal groups: groups of control, PCOS control and two groups with PCOS which are under treatment through aqueous extract of Artemisia absinthium (100 and 200 mg/kg). Polycystic ovarian syndrome was induced in PCOS control group and PCOS group under treatment, by single intramuscular injection of estradiol valerate (4mg/kg). Aqueous extract of Artemisia absinthium was intraperitoneal injection to PCOS treated groups, for 24 days. Normal saline was injected to control and PCOS control groups for 24 days. At the end of treatment period, serum levels of LH, FSH, Estradiol, Testosterone, TNF-α, IL-1β and IL-6 and levels of SOD, CAT, GPX enzymes and level of MDA in ovarian tissue were measured through ELISA method.
FINDINGS: Compared to PCOS control group, MDA tissue level, serum levels of LH, Estradiol, Testosterone and TNF-α, IL-1β and IL-6 cytokines in group under treatment with 200 mg/kg aqueous extract of Artemisia absinthium significantly decreased and serum level of FSH and levels of SOD, CAT and GPX antioxidant enzymes of ovarian tissue significantly increased (p

Background and Currently medicinal plants as immunomodulator or antimicrobial agents have gained interests in pharmaceutical researches. The aim of this study was to evaluate antibacterial activity, mutagenicity and proliferator effect of different parts of seven species of Artemisia genus on human lymphocyte cells of methanolic extracts.
In this experimental study the plant materials were collected, identified, air dried, and powdered. Then, the plant materials were extracted by methanol using maceration method. Antibacterial activity was determined by disk diffusion method. Also, mutagenic and proliferative activities of the extracts were evaluated by Ames test and MTT assay, respectively.
None of the species showed mutagenic activity. The extracts from the flowers and roots in all tested species showed high antibacterial and proliferative activities. Artimisia vulgaris and A. sieberi had the most and least antibacterial activity, respectively. On the other hand, A. khorasanica and A. deserti showed the highest and lowest proliferative effects on human lymphocyte cells, respectively.
In this investigation none of the species showed mutagenic activity. We also observed significant antibacterial and proliferative activities in flower and root extracts.

Hydatidosis is one of the dengerous zoonotic diseases that cause serious problems for human health, as well as major economic losses for livestock industry. Due to the nature of the parasite life cycle and also the structure of the cyst in human, the control of parasite in community is difficult and its treatment has faced with a major challenge. One of these challenges is inactivating the protoscolices for treatment purposes and preventing secondary cysts. Different chemicals have been used in the treatment of cyst that most of them had serious side effects for the patient. The aim of this study was investigating the scolicidal effects of some herbal extracts in vitro. Liver hydatid cysts were collected from slaughterhouse; the cysts fluid containing live protoscolex was aspirated aseptically and stored at 4°C until use. Three concentrations (25, 50 and 100 mg per ml) of each extract (ginger and artemisia) prepared and protoscoleces placed into incubator at 37oC. The viability of the protoscoleces was determined by eosin staining method at the times 5, 10, 25, 40 and 60 minutes. The methanolic extract of ginger at the concentration of 100 mg/ml leads to kill all of protoscoleces at 40 minutes. While the artemisia extract in none of investigated concentrations had not much effect on the protoscoleces. The study of animal models and complementary tests showed that methanolic extract of ginger can be used as an effective protoscolex for it has high activity.

Morphine withdrawal syndrome is one of the critical and dangerous side effects of opioid abusing; and treatment of addiction and its withdrawal syndrome are in health priorities. The aim of this study was to investigate the effect of Teucrium polium, Descurainia sophia and Artemisia aucheri extracts on morphine. In this experimental study, 40 male rats were divided into 4 identical groups including control group that received morphine, naloxone and distilled water, and groups with morphine dependent rats treated with Descurainia sophia extract (400 mg/kg), morphine dependent rats treated with Teucrium polium extract (600mg/kg), and morphine dependent rats treated with Artemisia aucheri extract (200mg/kg). All animals received different doses of morphine for 7 days. Then, at the 7th day of addiction they received the extracts by an oral-gastric catheter. After passing one hour of intraperitoneal naloxone injection (0.5 mg/kg), each animal was placed in a cage separately and observed for forty minutes. Then, variables of fecal quality, frequency, and weight of stool, lying down, itching, and jumping were determined. In this experimental study, the extract of Descurainia sophia significantly decreased indexes of lying down, weight loss and itching compared with control group (P<0.01). Teucrium polium extract group significantly decreased (P<0.01) frequency of jumping and lying down compared with control group. Also, the Artemisia aucheri extract decreased itching index compared to control group (P<0.001). Results showed that the extracts have significant effects on reducing the symptoms of withdrawal syndrome in rats. More studies are suggested to confirm the effects of these plants on morphine withdrawal syndrome.

Consumption of plant derived antioxidant compounds contributes to reducing risks of certain chronic and degenerative diseases. The present study aimed at comparing various antioxidative activities hydroalcholic of flaver extract (DKME) and essential oil of Artemisa persica in Lorestan province in 2012. The present experimental study was carried out in Lorestan province in 2012. After supplying hydroalcholic extract and essential oil of Artemisa persica، radical scavenging activity of the samples was assessed by using diphenylpicrylhydrazyl (DPPH). Total antioxidant capacity of the samples was assessed through phosphomolybdat method. The amount of total phenol and flavonoid samples was assessed by means of Folin-Ciocalteu and Zhishen methods. The obtained data was analysed using Kruskal-Wallis test and SPSS software (V: 13) at the significant level (P<0. 05). It was found that total antioxidant capacity of hydroalcholic extract and essential oil of Artemisa persica was 25. 123±5. 18 and 1. 05±0. 55 nmol of ascorbic acid equivalent/g extract، respectively. That of essential oil and phenol content was 326. 68±10. 07 and 131. 14±3mg of gallic acid equivalent (GAE) /g extract or essential oil، respectively. Flavonoid content of quercetin equivalents/g extract and essential oil was 6. 3±0. 94 and 1. 4±0. 65 mg، in the order mentioned. In the DPPH scavenging assay، the IC50 (the concentration required to scavenge 50% of radicals) values of hydroalcholic extract of Artemisa Persica leaves، essential oil and Butylated hydroxytoluene (BHT) as reference was 165. 73±4، 16470. 67±34، and 3. 88±1μg/ml، respectively. The current study showed that Artemisa persica extract is an easily accessible source of natural antioxidants which can be suitable to be included in foods and pharmaceutical applications.

Heavy metals cause to nephrotoxicity in kidney tissue of human and animals through increased production of oxygen free radicals. Plants Antioxidants are of good choices for protection against the toxicity of these compounds. In the current study، the protective effect of hydro alcoholic extract of Artemisia was assessed on Mercuric chloride-induced nephrotoxicity. In this study 28 male mice were used. Animals were randomly divided into 4 groups (N=7) as: 1 - Control، 2 - Mercuric chloride، 3 - Mercuric chloride with extract of Artemisia and 4 – Extract of Artemisia groups. Mercuric chloride and Artemisia extracts intra-peritoneal were injected with the dose of 1. 5 mg/kg and 200 mg/kg for 8 days، respectively. Then، blood samples were prepared and nephrotoxicity was determined by measuring serum urea and creatinine، using spectrophotometry (device SINCO) and specific kit (Pars test). Data were reported as Mean ± SEM via SPSS software. One-way ANOVA followed by Tukey test was used for data analysis and P <0. 05 was considered as a significant difference. Compared to the Mercuric chloride group، Artemisia extract caused a significant decrease in serum urea and creatinine in the combined Artemisia extract with Mercuric chloride group. Mercuric chloride also caused a significant increase in serum urea and creatinine in the Mercuric chloride group compared to the control group. Our results showed that the extract of Artemisia has protective effects against nephrotoxicity induced by heavy metals such as mercury.

Backround and

In the present time, analgesic and anti-inflammatory drugs such as nonseroidal anti-inflammatory drugs and opioid compounds are used for the releif of pain, but due to their side effects and economical issues, the significance of research on finding analgesic drugs with less side effects and their ability to substitute these synthetic drugs and substituting newer analgesic compounds is obvious. There is very little information about physiologic effects of Artemisia herba alba, one of species of Herba alba family. In the present study the possible analgesic effects of hydroalcoholic extract of this plant were investigated in chemical and thermal models of nociception in rats.

In this experimental study, percolation method was used for extraction. Specified amounts of dried extract were dissolved in twin to produce desired concentration. The extract were used orally or injected intraperitoneally and analgesic activity was assessed with two methods, Tail flick and Formalin test. In addition, anti nociceptive effect of this plant was compared with sodium salicylate as standard drug.

The plant extract significantly increased tail flick latency in tail flick test and decreased significantly pain perception in both phases of formalin test. The anti-nociception effect of herba alba was dose dependent.

These data confirm peripheral and central antianalgesic effect of this plant.

Considering the prevalence of leishmaniasis in different regions of the world including Iran and many side effects of pentavalent antimony compounds used in its treatment, and controversies about herbal drugs, the effects of Artemisia sieberi extract on the experimental ulcers of cutaneous leishmaniasis (CL) on BALB/c mice was studied at Kashan University of Medical Sciences. In this experimental study the effect of 1, 3 and 5 percent concentrations of Artemisia sieberi hydroalcohol lotions in mouse previously infected with alive and active Leishmania major promastogote was evaluated. The 50 selected BALB/c Mice were divided into 5 groups: 3 groups (n= 30) in experimental and 2 in control groups (n= 20). Three groups (cases) received drug one group received ethanol 80% and one group received none (controls). The drug was administered topically, three times daily for maximum of 30 days. Ulcer diameters were measured in all groups every 10 days and were tested for amastigote. Ulcer diameter change was analyzed by Paired t-test, ANOVA and Tukey. Whole recovery wasn’t observed in any treated mouse by Artemisia concentrates at the end of 30-day treatment period and mice direct smears in microscopic studies were positive. 1, 3 and 5 percent Artemisia extracts were ineffective in the treatment of Leishmania major ulcer. Artemisia emulsions had no effect on the reduction of ulcers diameter and leishman bodies at the end of treatment course.

History and Considering a prolonged treatment process for paronychia and a high complication rate for griseofulvin, in this study a case report on the treatment of pitted nail with Artemisia was introduced. Case Report: A 36-year old man (An inhabitant of Kashan) noticed thickening and development of congregations, fragility and a change in color (Yellowish brown) and appearance in nail of little finger of his right hand. With recommendation of a dermatologist, he referred to laboratory of mycology of Kashan University of Medical Sciences and his disease was diagnosed as dermatophytosis. Because his liver was hypo-functional and there was contraindications for griseofulvin and since Artemisia had fungi static properties prepared by Baridje Essence Corporation, therefore local treatment with drop extract of this plant was begun three times/day for a period of 15 weeks. After 14 weeks, the nail restored to normal and no complication was observed. Conclusion and Recommendations: Artemisia drop could effectively treat paronychia. Thus, it is recommended to carry out more studies in this field.