جستجوی مقالات مرتبط با کلیدواژه « میکروبیولوژی » در نشریات گروه « پزشکی »

One of the most common infectious diseases in diabetes mellitus is diabetic foot ulcer infection. This study was designed to identify the microorganisms that cause diabetic foot ulcer infection and their antibiotic resistance to improve the rational administration of antibiotics in experimental therapy.

The present descriptive cross-sectional study is retrospective. This study was performed on 200 patients admitted with a diagnosis of diabetic foot ulcer infection in the infectious ward of Razi Hospital in Ahvaz during the years 1394 to 1397. Finally, 126 of them were included in the study. Statistical analyzes were performed using SPSS software (v22) (IBM, USA).

In this study, 61 cases were female (48.4%), and 65 were male (51.6%). 18 cases (14.3%) were PEDIS 2, 71 cases (56.3%) were PEDIS 3, 37 cases (29.4%) were PEDIS 4. In this study, gram-negative bacteria 50.3% and gram-positive bacteria 49.7% were the cause of infection, and the three main strains were Staphylococcus aureus (41.3%), Escherichia coli (27.8%), and Staphylococcus epidermis (24.6%). 9.6% of Staphylococcus aureus were resistant to methicillin, and 5.7% of Escherichia coli were punk resistant. The lowest resistance was observed in gram-negative and positive bacteria to Meropenem and imipenem and the highest resistance to ciprofloxacin and clindamycin antibiotics.

it is necessary to study the causative strains and their antibiotic resistance alternately in each region. Progressive resistance to clindamycin and ciprofloxacin also reduces the effectiveness of these drugs in experimental therapies.

Dental units are among the equipment that flows through the dental application, and due to the durability of water and the probability of forming the biofilm. They can have a high microbial load. It is important to investigate this pollution, which should be frequently investigated due to the incidence of dangerous infections in people with weakness of the defense system, pollution and dental units. The aim of this study is to investigate the amount of bacterial infection and pollutant of Water Supply in Dental units at Bojnourd dental school 2017.

This study was conducted by descriptive _ analytical method on the water units in Bojnourd dental school.30 active units of the College of Dentistry were selected for the sampling. The samples were cultured on proprietary environments, and after 48 h at 37 ° C the number of microbial colonies was counted. And finally the obtained data were analyzed by SPSS 20.

The results showed that the average total bacterial count before flushing and after flushing was 711 and 293 cfu / ml, respectively, which had the highest level of contamination in water before flushing.

The results of this study concluded that flushing was one of the best methods to reduce microbial contamination and Considering that dental units at Bojnourd dental school are polluted, dentists should pay more attention to prevent infection from infected patients and dental staff.

Approximately 500 bacteria species inhibit in periodontal pockets. Association between Aggre gatibacter Actino my cetem comitans and destructive and progressive forms of periodontitis has been demonstrated. The aim of this study was to evaluate the microbiological and clinical effects of metronidazole-ciprofloxacin therapy in subjects with periodontitis. In this experimental randomized double-blind controlled clinical trial, 24 patients with at least 4 sites with ≥4mm of clinical attachment loss, bleeding on probing and detection of A. a were included. Measurement of Clinical attachment loss, gingival index, plaque index and bleeding on probing were done. All the patients received scaling/root planning and oral hygiene instruction. The patients received drug and placebo. Bacterial culturing and measurement of Clinical attachment. Attachment loss, gingival index, plaque index and bleeding on probing were done at the 10days, 3 and 6 months later. Data were subjected to Paired t-test and Mann-u-Whitney test for data analysis. Bleeding on probing, Gingival Index and A. a Colony count on 3rd and 6th month in control and test group were (33/337 و /5) Vs (0), (1/2±0/46-1/21±0/42 Vs(0/06 ±0/11-0/10±0/13), (9/15±10/88-12/56±11/35) Vs (0/75±0/99 -1/96±2/15) respectively. These differences were statistically significant (p<0/05). No significant difference was seen between two groups in Clinical attachment. Attachment loss and plaque index in all studied periods also gingival index, colony count in10 days showed no significant difference. Administration of Ciprofloxacin plus Metronidazole as an adjunct to mechanical therapy can provide effective periodontal healing and Aa eradication from periodontal tissue.

Severe corneal ulcer is an ocular emergency. The aim of this study was to identify the predisposing factors, microbial isolates and therapeutic results of patients with corneal ulcer. In a prospective study, between" 2003-2004" all patients with corneal ulcer, who admitted to Nikookari hospital, were evaluated by corneal scraping. All samples were studied by gram staining and micro biological culture. Corneal scraping was performed in 100 patients (M/F = 65/35, 51-80 years). Thirty one percent were farmer. Previous corneal surgery had been performed in 30% of patients. The most common organism in gram stain was staphylococcus Epidermidis (58%) and topical Cephazolin and Gentamycin were used in 89% of cases. Corneal ulcer was healed in 74% of patients with medical therapy. In this study, staphylococcus epidermidis was the most common organism and previous eye surgery was the most predisposing factor for development of corneal ulcer.

The aim of present investigation was to evaluate low frequency low-level infra red diode laser therapy for a third degree burn healing in rats skin. 36 rats were divided into groups one and two.On day zero three third degree burns were made on the dorsum of each rat by steam.In group one first burn were exposed to a 80 Hz –pulsed 890 nm infra red diode laser with o.396 J/cm2three times a week. In group two first burn were exposed to the laser with shot down laser. In both groups second burns were treated with topical application of nitrofurazone % 0.2. In both groups third burns did not receive any treatment and were considered as control burn. Burns were clinically examined. Wound closure of burns were recorded and compared by paired student t test. On days 812 and 20 microbiological samples of burns were collected and analysed.The data of each extracted micro organisms of burn were compared.A further comparison were made between micro organisms assumed to be pathogen and micro organisms presumed to be non-pathogen.Data were analyzed by chi-square method. Paired student t test showed wound closure rate of laser-treated burns(17.6±days)was significantly higher than that of its control burn(19.6±days)(p=0.007).There were not significant difference between each micro organism and also between micro organisms assumed to be pathogen and micro organisms presumed to be non-pathogen. Low-level laser therapy with a diode laser significantly accelerated third degree burn healing in rat.

Ventilated_associated pneumonia is the most common acquired infection in ICU.The prevalence of pneumonia in intubated patients is 21 times more than other patients.Tracheal tube colonization is considered as a risk factor and affected with duration of intubation and decreased gastric acid.This study is conducted to determine the epidemiology and microbiology status in the patients admitted at loqman hospital poisoning ICU. The reaserch method was descriptive and it's technique was an observation-interview.Patients with positive endotracheal tube culture were entered the study, the sampling method was endotracheal aspiration.Qualitative culture and resistance pattern evaluation with Kirby-bauer method was used. We surveyed 180 intubated patients with mean age of 31.63±13.9 years old. 83.3 precent of the patients had recieved antibiotic therapy before sampling. Staph. coag. positive.(45.6%)was the most commonly isolated pathogen and followed by Klebsiella, Acintobacter and E.coli. Isolation of one organism was seen in 62.8% and multiorganisms in 37.2%. Temp≥38 was seen in one_third of the patients and WBC≥12000 was detected in 44 precents of the patients. There was a significant relationship between antibiotic therapy with fever. 11.1 precent of the patients died. There was a significant relationship between the kind of organism and survival, but there was a significant relationship between antibiotic therapy before sampling and patient's survival. Detection of Acintobacter with Klebsiella, E.coli and Staph. aureuse increases the probability of nosocomial pneumonia caused by AST III group. Lack of relationship between kind of organism and prior antibiotic therapy is considered as an alarm for resistant infections.

Septic discitis is an inflammatory process of the intervertebral disc which usually involves the discovertebral junction and may extend in to the epidural space and para spinal soft tissues. The aim of this study was to evaluate spondylodiscitis cases(clinical, microbiological, radiological, treatment and complication features) in Firoozgar and Rasool-e-Akram hospitals during 1998-2003. Patients and The present research was an observational study done in periodic cross-sectional way on 50 patients with spondylodiscitis. Data collection including age, gender, predisposing factors, radiography, CT.Scan, MRI, CBC, ESR, CRP was done. The results of aspiration or biopsy, prescription of drug and etc. was recorded through a checklist. Statistical analysis was done with spss 11.5 software. Fifty cases of spondylodiscitis were identified, 25 patients were women(50%). The mean age of our patients was 48.20±20.70 years 75 percent of patients were aged ≤65 years. The most common presenting symptom was back pain(86%). ESR was increased in 91.1% of patients. Diagnosis was originally made by MRI. TB spondylodiscitis was the commonest form of disease. The major predisposing factors were invasive procedure and frequent UTI(22% each of them). Spondylodiscitis should be considered in any patient who has severe localized pain at any spinal level, especially if accompanied by fever and elevated ESR. In developing countries such as Iran one of the important causes of this disease is Tuberculosis.