جستجوی مقالات مرتبط با کلیدواژه "وروکوس کارسینوما" در نشریات گروه "پزشکی"

Squamous cell carcinoma is the most common type of oral cancer that shows certain tissue changes. Laminin-5γ2 is a protein that plays a central role in migration of the neoplastic cells during tumor invasion. Expression of this protein in cells and stroma adjacent to tumor can confirm the cellular invasion. The aim of this study was to evaluate stroma in verrucous carcinoma and squamous cell carcinoma of the mouth using two markers α SMA and laminin 5 gamma 2.

In this retrospective descriptive-analytical study, 60 samples were selected with the diagnosis of squamous cell carcinoma and verrucous oral carcinoma. Then, two markers of α SMA and laminin 5 gamma 2 were examined in epithelium and mesenchymal stroma by immunohistochemistry. The vasculature was used as a positive immunoreactivity control for alpha-smooth muscle and the normal mucosa was used to control the positive immunoreactivity of laminin 5 gamma 2. The results were analyzed using Chi-square and Spearman correlation tests (α = 0.05).

Out of 31 SCC samples, 80.6% showed laminin 5 gamma 2 and 90.3% showed alpha smooth and out of 9 samples of varocus carcinoma, 66.7% showed laminin 5 gamma 2 and 77.8% showed alpha smooth actin. The higher the positive percentage of laminin, the higher the percentage of positive α SMA.

Expression of alpha smooth muscle actin and lamminin 5 gamma 2 was significantly higher in oral squamous cell carcinoma and there exists a correlation between expression of alpha smooth muscle actin and the increase in myofibroblasts takes place during the carcinogenesis process.

Oral Squamous Cell Carcinoma (OSCC) is one of the most prevalent cancers of oral cavity worldwide. The main components consisting basal lamina are collagen type IV and adhesive glycoprotein lamina. Local loss of basement membrane leads to formation of laminin-5, gamma-2 (laminin–5γ2) through proteolysis of basement membrane or synthesis in cytoplasm of neoplastic cells. Laminin–5γ2 is a protein that plays a central role in migration of the neoplastic cells during tumor invasion. Expression of this protein in cells and stroma adjacent to tumor can confirm the cellular invasion. The aim of this study was to evaluate expression of laminin–5γ2 in OSCC, verrucous carcinoma (VC), and dysplastic mucosa (DM), compared to normal mucosa (NM).
57, 31, 9,9, and 8 paraffin blocks of squamous cell, OSCC, VC, DM, and NM (as control group) were scored as laminin-5γ2 markers, respectively. The 10 and 40-fold magnifications were used to determine the percentage of colored cells. [Zamen1] All the data were statistically analyzed by Chi-square and Mann-Whitney tests using SPSS version 20.
the findings demonstrated that expression of laminin marker was positive in the epithelium of 61.3, 33.3, and 22.2% of the SCC, VC, and DM samples, respectively. On the other hand, it was negative in 100% of the NM samples. The distribution frequency of negative and positive laminin samples was assessed based on the type of lesions. The significance level was considered as 0.006, which is less than 0.05. There was a significant difference concerning the number of laminin positive lesions between the types of oral malignancies and normal mucosa. The SCC lesions revealed the highest positive results.
According to the results of this study, laminin–5γ2 expression in the basement membrane, extracellular matrix, and epithelium of malignant tumor tissue might indicate the invasive and metastatic behavior of the OSCC