جستجوی مقالات مرتبط با کلیدواژه "پارانونایل فنل" در نشریات گروه "پزشکی"

Para-Nonylphenol (p-NP) is an estrogenic compound with toxic effects on the various organs including testis. The purpose of this investigation was to study the toxic effects of p-NP on the testis tissue of mice and evaluation of the effect of Nigella sativa oil (NSO) against this toxicity
This study was conducted on 24 adult male NMRI mice (32±3 g) that were divided into 4 groups (n=6), control, NSO (5mg/kg/day), p-NP (250mg/kg/day) and p-NP NSO, and treated orally for 34 days. Finally, mice were sacrificed, their right testis were taken out, fixed, sectioned, processed and stained using Heidenhain̕ & azan method. Testicular tissue sections were evaluated using stereological method. Lipid peroxidation index, malondialdehyde (MDA) level, was also measured in serum of mice. The data were statistically analyzed using one way ANOVA and difference means was considered significant at P A significant decrease in the diameter (P NSO has a protective effect on the p-NP toxicity in the mice testis therefore it can be suggested as a raputic supplement in preventing the adverse effects of p-NP exposure in industrial zones.

Para-Nonylphenol (p-NP) can cause oxidative stress through reducing the activity of scavenging enzymes. The aim of the present study was to investigate the effect of green tea extract, as an antioxidant, on testis in rats exposed to p-NP. In the present study, 24 Wistar rats with mean body weight of 215 ± 10 g were divided into 4 groups (n = 6). The first group was considered as the control group. The treatment group, respectively, received p-NP (200 mg/kg/day), green tea extract (200 mg/kg/day), and p-NP + green tea extract orally for 56 days. Then, the rats were sacrificed and their right testis removed. After tissue processing and staining, the volume of seminiferous tubules and interstitial tissue, seminiferous tubules length and diameter, height of germinal epithelium, basement membrane thickness, and the number of cell types were estimated stereologically. The concentration of serum malondialdehyde (MDA) was also evaluated. Data were analyzed using one-way ANOVA. All P values of less than 0.05 were considered significant. The mean volume and diameter of seminiferous tubules, the thickness of their basement membrane, germinal epithelium height, and mean total number of spermatocytes, spermatids, and Sertoli cells significantly reduced in the p-NP group compared to other groups. MDA concentration in rats treated with p-NP significantly increased compared with the control group. Green tea seems to have a protective role in p-NP-induced toxicity in testis tissue. Thus, the results suggest that consumption of green tea, as an antioxidant agent, can play an important role in preventing the side effects of p-NP exposure in industrial areas.

Para-nonylphenol (p-NP) is an important environmental pollutant that can affect the male reproductive system through endocrine disruption and inducing of oxidative stressence. Hence, this study aimed to investigate the protective effect of Nigella sativa oil (NSO) on testicular tissue and sperm parameters (count, motility, viability and morphology), sperm DNA and chromatin abnormalities against toxicity induced by p-NP in adult NMRI mice.

Twenty-four adult male NMRI mice (32±3g) were divided into 4 groups (n=6): control, NSO (5ml/kg/day), p-NP (250mg/kg/day) and p-NP+NSO. After 34 days of oral treatment, the left testis was removed and used for histopathological observations. Left caudal epididymis was cut in the Ham’s F10 and released spermatozoa were utilized in order to analyze sperm motility, viability, number and morphology. Sperm chromatin quality was assessed by nuclear staining using acridine orange and aniline blue. The blood serum malondialdehyde (MDA) levels were also measured. The study data were analyzed utilizing one Way ANOVA and Tukey’s test, and P<0.05 was considered as the significant level.

A significant decrease was observed in the p-NP group in regard with the motility, viability, number, normal sperm morphology, diameter of seminiferous tubules and germinal epithelium thickness, whereas a significant increase was reported in regard with the diameter of the seminiferous tubules lumen and MDA levels compared to the control group. Above parameters were significantly compensated by Nigella sativa oil in p-NP+NSO group compared to Para-nonylphenol group. The application of Nigella sativa oil alone significantly increased the number, motility, normal morphology of the sperm and significantly decreased the MDA levels compared to the control group.

The study results indicated that the Nigella sativa oil, as a potent antioxidant, could compensate for the toxicity induced by p-NP.

Para-nonylphen as an environmental pollutant has weak estrogenic activity and causes oxidative stress in different organs including testis. This study was done to determine the protective effect of vitamin E on the para-nonylphenol induced-testicular toxicity in adult rats. In this experimental study, 24 Wistar rats were randomly allocated into four groups including control, vitamin E (100 mg/kg/day, orally), para-nonylphenol (250mg/kg/day, orally) and finally para-nonylphenol (250mg/kg/day, orally) plus vitamin E (100mg/kg/day, orally). After 56 days of treatment, removal of the right testis, tissue processing and staining with Heidenhain's Azan, the morphometric parameters of testicular tissue was evaluated using stereological method. The mean volume of seminiferous tubules, height of the germinal epithelium, seminiferous tubules diameter, thickness of the basement membrane, number of spermatocyte, spermatid and sertoli cells significantly reduced in para nonylphenol group compared to the controls (P<0.05). These parameters were significantly increased in the para-nonylphenol plus vitamin E group compared to para nonylphenol group (P<0.05). In the histopathological examination, atrophy of seminiferous tubules, germinal epithelium vacuolation and epithelial disarrangement were observed in para nonylphenol group. Histopathological alterations reduced in para-nonylphenol plus vitamin E group compared to para nonylphenol group. Co-administration of vitamin E with para nonylphenol can prevent the adverse effects of para nonylphenol on the testicular tissue in adult rats.

In this study, the effect of para-nonylphenol as an environmental pollutant on viability, morphology and proliferation of bone marrow mesenchymal stem cells was investigated. Bone marrow mesenchymal stem cells of rat were treated with the 0.5, 1, 2.5, 3.5 and 5 μM of paranonylphenol for a period of 21 days, then the viability of the cells were estimated using trypan blue and MTT methods. After choosing the effective dose, the integration of the DNA was investigated using comet assay and agarose gel electrophoresis. Mechanisms of cell death were also investigated by TUNEL assay and presence of caspase activity. The results showed that para-nonylphenol caused significant dose dependent reduction of viability and proliferation of the cells. Comet assay, agarose gel electrophoresis and TUNEL test showed that the DNA of the cells were damaged and broken after treatment with 0.5 and 2.5 μM of para-nonylphenol. In addition, activated caspase-3 was observed in the cytoplasm of treated cells. This study showed that a very low concentration of para-nonylphenol has drastic effects on bone marrow mesenchymal stem cells. This chemical is used in formulation of cosmetics and detergents and therfore may have detrimental effects on the viability and proliferation of stem cells.