جستجوی مقالات مرتبط با کلیدواژه « پونه » در نشریات گروه « پزشکی »

The medical application of oil-in-water nanoemulsion has been expanded due to its special properties. Nanoemulsions based on medicinal plants are important because of the simple and fast preparation method, high efficiency, and economic efficiency.

In this study, the oil-in-water nanoemulsion was prepared containing Mentha pulegium essential oil as the oil phase, Mentha pulegium extract as the aqueous phase, and span 80 surfactant for 30 minutes using homogenizer. The properties of samples were investigated. Chitosan was used as the natural biological material to modify the optimal nanoemulsion due to its biocompatibility, biodegradability, and antibacterial properties. The samples were evaluated by methods of dynamic light scattering (DLS) to investigate size and size distribution, transmission electron microscopy (TEM) to measure size and shape, and zeta potential to determine stability. Antibacterial activity, antioxidant activity, and cytotoxicity of the samples were investigated.

The results of the antibacterial activity of the essential oil, its extract, and nanoemulsions against gram-negative bacteria escherichia coli (E.Coli) and gram-positive bacteria staphylococcus aureus (S.Aureus) showed that these materials are suitable candidates instead of chemical drugs to deal with strains. The antioxidant activity of the samples was observed based on the measurement of total phenolic compounds and free radical inhibition. Also, the samples are not cytotoxic on healthy cells.

Based on the results, oil-in-water nanoemulsion containing essential oil as the oily phase and extract as the aqueous phase can be suitable candidates for pharmaceutical and medical applications.

Considering the antibacterial effects of medicinal plants and the side effects of chemical drugs, replacing chemical drugs with herbal drugs is of particular importance. Therefore, this study aims to investigate the chemical compounds in the extracts of Uritica dioica L., Mentha longifolia L., and Tragopogon collinus collected from the vegetative regions of Meshgin Shahr and compare the therapeutic effect of their combination with that of ciprofloxacin against common bacteria of urinary tract infection.

The main phenolic compounds of the desired medicinal plants were identified using HPLC after collecting from the original habitat and extraction by the maceration method. Then, the antimicrobial properties of the combination of extracts against common urinary tract infection bacteria were investigated and compared with those of the ciprofloxacin drug using the disk diffusion method. In addition, the MIC values of the combination of extracts at concentrations of 12.5, 25, 50, and 100 mg/ml against the studied bacteria were determined in 3 replicates.

The main phenolic compounds identified from the hydroalcoholic extract of Uritica dioica L,. including catechin and rutin, Mentha longifolia L., including gallic acid and quercetin, and Tragopogon collinus including ursolic acid and caffeic acid. According to the obtained results, it was found that the mixture of plant extracts shows relatively similar antimicrobial properties   to ciprofloxacin.

According to the obtained results, it can be said that the mixture of extract of Uritica dioica L., Mentha longifolia L., and Tragopogon collinus medicinal plants by conducting more studies, especially at the in vivo conditions, can be a suitable product for treatment of urinary tract infection.

Nowadays, the application of procedures to minimize oxidative and microbial spoilage in marine products is economically and hygienically important. This study was performed to determine the effect of free and nanoliposome forms of poneh (Mentha pulegium L.) essential oil to increase the shelf life and improve the sensory characteristics of silver carp minced fish (Hypophthalmichthys molitrix) during storage time.

In this study, the antibacterial properties of 1 and 2% essential oil and nanoliposomes form on the microbial population of minced silver carp fish inoculated with Bacillus cereus and Escherichia coli were measured. Also, chemical tests (DPPH (2,2-Diphenyl-1-picrylhydrazyl assay, measurement of iodine peroxide (PV), pH, total volatile nitrogen (TVB-N) and thiobarbituric acid (TBARs)) and sensory properties of minced silver carp were evaluated during 12 days of refrigerated storage.

The results showed that the addition of poneh essential oil (free and nanoliposomes) according to the microbial results and the results of chemical experiments can effectively prevent microbial growth and chemical spoilage (p<0.05). Treatment containing 2% nano essential oil of poneh showed the greatest effect on the chemical, microbial and sensory properties of minced fish during the experimental period in comparison with control treatments, 1% poneh nanoliposomes essential oil and 2 and 1% free essential oils.

Therefore, the nanoliposomes form of 2% poneh essential oil can be used as a suitable additive to increase the shelf life of Silver carp fish in the fish industry.

Despite numerous treatments, head lice are still a health problem, especially in female students. In this study, the effect of topical application of Allium Sativum and Mentha Pulegium on the severity of head lice infection has been investigated.

A multi-stage randomized clinical trial study was performed on 120 female primary and secondary school students with head lice in Gonabad. After obtaining written consent from parents and oral consent from students, a demographic questionnaire and a checklist were completed. One group was given a decoction Allium Sativum and Mentha Pulegium and the other group was given 4% dimethicone lotion twice a week. Evaluation was made before, 7 days and 14 days after the intervention.

The severity of lice infection during the interventions in both groups decreased significantly (P=0.001), but the frequency of severity of infection before, 7 days after and 14 days after intervention was not statistically significant in the two groups.

Our findings suggest that both Allium Sativum and Mentha Pulegium can be used for the treatment of head lice infection.

Breast cancer is one of the most important neoplasms affecting one out of eight women. Despite new therapeutic methods, the breast cancer mortality rate is increasing annually. Medicinal plants are considered important sources of anti-cancer compounds. The present study was conducted to evaluate the cytotoxic effects of hydroalcoholic extract of Mentha Pulegitim L. and Satureja hortensis L. on MCF-7 and MCF-10 breast cancer cell lines in vitro.

This study was performed in the laboratory of Boroujerd Azad University, Boroujerd, Iran, in 2020. After the preparation of hydroalcoholic extracts of Mentha Pulegitim L. and Satureja hortensis L., the effect of different concentrations of 3, 7, 15, 30, 60, 125, and 500 g/ml of both plants were examined on cell lines prepared at the tumor section of the Department of Cell Bank of Pasteur Institute of Iran, Tehran, Iran, for 24, 28, and 72 h. The effect of cytotoxicity was evaluated using an MTT assay. The light absorption was determined at 570 nm wavelengths using an enzyme-linked immunosorbent assay reader. In this study, a one-way analysis of variance was utilized. The identification of the compounds was performed using the Gas Chromatography-Mass Spectrometry method.

The highest percentages of inhibition of MCF-7 and MCF-10 breast cancer cell lines were found in the 500 μg/ml concentrations of the hydroalcoholic extracts of Satureja hortensis L. and Mentha Pulegitim L.at 96.558% and 88.547%, respectively.

The results of the present study showed that the hydroalcoholic extracts of Satureja hortensis L. and Mentha Pulegitim L. leaves had significant cytotoxicity effects on the breast cancer cell line and prohibited their growth extensively.

Considering the adverse effects of synthetic antioxidants, anticancer drugs and antibiotics on human health, in this study, bioactivities of Mentha longifolia essential oils collected from Marivan and Qaza An were examined and compared with each other for identification a natural alternative of synthetic antioxidants, anticancer drugs and antibiotics.

Essential oil of M. longifolia leaves and stems collected from Marivan and Qaza An was prepared using solvent-free microwave extraction method. The essential oil components were identified by gas chromatography/mass spectrometry. The antioxidant and cytotoxicity activity of the essential oils were determined via β–carotene bleaching assay and brine shrimp lethality test, respectively. The antimicrobial activity of the essential oils was evaluated by the agar well diffusion method and by determination of minimum inhibitory concentration.

The main components of M. longifolia essential oil collected from Marivan were pulegone (81.45%) and isopulegol (8.39%) and the main components of M. longifolia essential oil collected from Qaza An were pulegone (48.29%) and piperitenone oxide (23.53%). The inhibition of linoleic acid oxidation by M. longifolia essential oils collected from Marivan, Qaza An and BHT were 80.02%, 78.59% and 95.50%, respectively. The LC50 of the M. longifolia essential oils collected from Marivan, Qaza An and vincristine sulfate were 53.47μg/ml, 55.96 μg/ml and 0.751 μg/ml, respectively. M. longifolia essential oils had good antimicrobial activity, especially against gram-positive bacteria.

M. longifolia essential oils have significant antioxidant, antimicrobial and anticancer activity and can be introduced as alternatives of synthetic antioxidants, antibiotics and anti-cancer drugs.

Anxiety is one of the most common psychiatric disorders that leads to poor quality of life and chronic disorders. Regarding the side effects of most chemical drugs, this study was conducted to evaluate the effect of hydroalcoholic extract of Mentha pulegium leaves on anxiety in male rats.

In this experimental study, 24 male rats were used. Animals were divided into 4 groups (n=6), including Control groups (untreated) and three experimental groups receiving doses of 400, 800, 1600 mg/kg/w of Mentha pulegium leaves hydroalcoholic extract. The extract was injected intraperitoneally half an hour before the test. Anxiety was assessed by using elevated plus maze. The duration and frequency of the presence of the rats in open arms and closed arms of maze were recorded. Data were analyzed by one-way ANOVA and Tukey post-hock tests.

The mean percentage of open arms and their duration was significantly increased in rat receiving the extract compare than control (P <0.05), and the duration of presence in closed arm was reduced by the extract (P <0.05, 0.01).

According to these findings, Pune's hydroalcoholic extract reduces anxiety in rats and may have this effect on humans’ witch it need to be studied

Food poisonings caused by consumption of domestic dairy products such as cheese due to Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) are one of the hygienic problems in foodstuffs. Nowadays, using natural preservatives instead of synthetic preservatives in foods has been regarded increasingly and among these natural compounds, medicinal plants comprise these materials that finding their antimicrobial effects have high priority. The aim of this study was to investigate antibacterial effect of Thyme (Thymus persicus (and Pennyroyal (Mentha longifolia) alcoholic (ethanolic) and aqueous extracts against isolated bacteria from domestic cheeses. Material & Methods: After preparing Thyme and Pennyroyal, alcoholic (ethanolic) and aqueous, the extract of these plants were gained and their antimicrobial effects were investigated in concentrative levels of 0.39- 100 percent against E. coli and S. aureus isolated from domestic cheese by microdilution method. E. coli PTCC 1270 and S. aureus PTCC 1112 were used as control. Alcoholic (ethanolic) extract of Thyme and Pennyroyal showed more antibacterial effect against Staphylococcus aureus in comparison with Escherichia coli (P<0.05). Thyme ethanolic extract showed more antibacterial effect than Pennyroyal ethanolic extract (P<0.05). Aqueous extract of Thyme and Pennyroyal did not show any significant antibacterial effect in tested concentration (P>0.05). According to current study results, alcoholic (ethanolic) extract of Thyme and Pennyroyal have high antibacterial potency and they can be used as alternative instead of synthetic preservatives for protection against food borne bacterial poisoning and infection.

Marinated olives using various herbs, spices and pastes have become quite popular and demanding. This research work is concerned in providing new formulations of marinated olives using various concentrations of tamarind and oregano. Chemical evaluations concerned with pH, acidity, salt and antioxidant activities and microbiological evaluations concerned with lactic acid bacteria, molds and entrobacter followed by sensory evaluations related to aroma, color, taste and texture were carried out according to the defined standard. The results indicated a decrease in pH and consequently an increase in acidity with a statistical analysis of 95% (p<0.05) using Duncan procedure. Both pomegranate paste and tamarind contain high concentrations of phenolic compounds that could act as primary antioxidants. By increasing tamarind concentration in relation to pomegranate paste, lactic acid bacteria were observed. The application of dried oregano increased molds growth as compared to the control without oregano. Salt concentration within the permitted limit had little effect on the growth of microorganisms. The samples containing 10% tamarind and 1% oregano scored the highest regarding the taste and flavor of the samples. The texture and color of the formulated product were not affected during 120 days of storage at 4 C.

Zero- iron nanoparticles, due to their high degradation properties, cause the destruction of many pollutants. Among the methods for synthesizing iron nanoparticles, green synthesis using plant extracts is more beneficial for the synthesis of nanoparticles than other methods.
In this research, zero iron nanoparticles are synthesized in a one-step process by using of Mentha longifolia L. extract, which includes phenolic compounds, from the reduction of iron (III) chloride salt. The purpose of this research is to synthesize and develop a green method for the preparation of zero iron nanoparticles.
The extract was obtained by percolation method at room temperature and distilled water solvent. The extract was added to a FeCl3.6H2O iron solution of 0.01 M. The color change from yellow to dark brown indicates the formation of nanoparticles. Changing in pH and color of the solution after the start of the reaction were investigated and recorded. Specifications of the synthesized nanoparticles were analyzed using SEM (scanning electron microscopy), UV-vis and FTIR.
Iron nanoparticles were synthesized at 46°C, 87 min and the volume ratio of 0.01 M iron salts to the extract 5:1. In this condition, the nanoscale size was 30.44 nanometers, which was confirmed by the SEM image.
The synthesis of zero-iron nanoparticles using Mentha longifolia L. extract is possible. The antioxidant compounds in the plant can reduce iron (III) and produce zero iron nanoparticles.

Aflatoxins are natural fungal toxins produced by Aspergillus species such as A. flavus. The toxins are poisoning and can cause tissue necrosis and liver cancer. The aim of this study was to determine the control of Aflatoxin B1 production by extracts and essential oils.
Aqueous extracts were prepared by heating and essential oil by Clevenger's apparatus. Antifungal activity of essential oil and aqueous extract of Mentha pulegium and Satureja hortensis were determined by disc diffusion and microplate dilution methods. Production control of Aflatoxin B1 was investigated with concentrations under MIC(Minimum inhibitory growth concentration) of two materials and were determined by HPLC method.
The most zone of inhibition was 10% belonging to Satureja essential oil and its aqueous extracts with diameters of 26mm and 12mm, respectively. These values for Mentha extract and 10% essential oil were 18mm and 8mm respectively. MIC of the aqueous extract of Satureja and Mentha were 0.031 and 0.063mg/ml respectively, and 1% essential oil of two materials was 0.039 and 0.078 mg/ml, respectively. Aflatoxin B1 produced by A. flavus in concentrations of 1%, 2% and 10% Satureja essential oil were 122, 113 and 134 ppb, in 1%, 2% and 10% Mentha were 163, 168 and 171 ppb, respectively. The aqueous extracts of 1% Satureja reduced the production of toxin as 58.1 and the 1% aqueous extract of Mentha as 39.6.
The results of this study showed that both Satureja hortensis and Mentha pulegium have the ability to inhibit the growth of Aspergillus flavus fungus, as well as control of aflatoxin B1 production in low concentrations and recommended for further studies.

Mentha pulegium L. is one of the medicinal plants used in the food production as a flavoring substance and its antibacterial effect has also been considered. The present study was designed to evaluate the phytochemical composition of Mentha pulegium L. originated from Iran and its antimicrobial activity against several pathogenic bacteria.
The chemical compounds of this medicinal plant were analyzed by GC-MS and 14 components were identified, representing 86.98% of its total essential mass. The antibacterial activity of the essential oil (EO) of Mentha pulegium L., has been evaluated against Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, and Pseudomonas syringae pv. tomato using disc diffusion method compared to synthetic antibiotic ciprofloxacin. The minimum inhibitory concentration (MIC) was evaluated against the above tested bacteria using macrobroth dilution method.
Results showed that the EO of Mentha pulegium L., was formed by the main components of piperitone (32.1%), piperitenone (21.71%) and pulegone (15.85%). In addition, the results obtained using MIC and MBC showed that the minimum inhibitory concentration of the EO of M. pulegium was in the range of 10-40 mg/mL for S. aureus, E. coli, P. aeroginosa and 20 mg/mL for Ps. syringae pv. tomato, respectively. According to the results, the maximum zones of inhibition (29.15 mm) was observed on P. syringae P. tomato in 40 mg/mL of essential oil, and the minimum zone of inhibition (17.08 mm) was related to P. aeroginosa in the same concentration.
The results revealed that EO of Mentha pulegium L. had an appropriate inhibitory effect on Staphylococcus aureus and Escherichia coli.

Resistance of Acanthamoeba cysts causes recurrence of the disease; so, the patient should be monitored regularly ¡The aim of the study was to examine the effect of a few herbal materials on Acanthamoeba cysts in vitro.
Essential oils (EOs) of Zataria, Mint, and Oregano were prepared by steam distillation. The EOs and Hypericum perforatum extract were prepared in three concentrations (0.6%, 1% and 10%)¡ Acanthamoeba cysts in various time intervals (30, 60, 120, 180 and 1440 minutes) were exposed with plant extracts. Then, the viability of parasite was investigated by eosin 0.1%.
Comparison of the parasite mortality rate between control and case groups showed that the mortality of Acanthamoeba cysts was higher in the case groups that exposed to herbal materials. At the equal concentration (10%) and time (24 h), the Zataria and mint EOs produced the highest (22%) and lowest (4%) mortality, respectively. The results showed the mortality rate of Acanthamoeba was time-dependent.
Zataria showed the most fatality effect against Acanthamoeba cysts. In this respect, clinical trial studies are suggested.

Ephedra, Nepeta, and Hymenocrater herbs have long been used in the treatment of many diseases, but their interactions with cellular components, especially nuclear proteins, are still unknown. On the other hand, in the eukaryotic cell nucleus histone proteins play a main role in the packaging of the genetic material as chromatin. The present study aimed at comparing the in-vitro interactions of aqueous extracts of Ephedra, Nepeta, and Hymonocarater with histone proteins.
Histone proteins type 2A were purchased from Sigma company and aqueous extract of Ephedra, Nepeta and Hymenocrater were prepared in the research laboratory of Birjand University of Medical sciences. Different concentrations of the aqueous extracts were incubated with histone proteins, then analyzed by UV-Spectroscopy and Circular dichroism.
Aqueous extract of Nepeta increased maximum absorbance of histone proteins at 210 nm, but the reduction in absorbance was shown at its high concentration. The absorbance of histone proteins also changed in the presence of the aqueous extract of Ephedra and Hymenocrater and it decreased to zero at higher concentration of Ephedra. Circular Dichroism studies demonstrated that the structure of histone proteins changed in the presence of mentioned aqueous extract; the observed effect of the aqueous extract of ephedra was higher than Nepeta and Hymenocrater.
The aqueous extracts of ephedra, Nepeta,and Hymenocrated interacted with histone proteins and changed their structure. The effect of ephedra was higher than others.

Low back pain is one of the most common and most costly chronic diseases, which in many cases there is no cure for it . Iranian Traditional Medicine (TIM), has a valuable therapeutic perspectives for the treatment of low back pain. According to Iranian traditional medicine (TIM), One of the main causes of low back pain is dyspepsia. The aim of this study (by conducting a clinical trial) is to investigate the hypothesis that treatment of dyspepsia can reduce low back pain symptoms.
30 patients between 20 and 55 years with chronic low back pain for more than 12 weeks were diagnosed according to the criteria ROM3 suffering from dyspepsia were enrolled.
Mentha longifolia capsules as a drug with positive effect in dyspepsia were given 3 times daily after meals for 4 weeks At the time of entry and after weeks 2, 4 and 8. Visual analog pain scale(VAS) and Oswestry Functional Disability Questionnaire were used and severity of dyspepsia Leeds Questionnaire were checked out.
Mentha longifolia capsule significantly reduced dyspepsia, and also pain and disability due to low back pain. (p Mentha longifolia capsule can reduce pain and disability by reducing dyspepsia. It is suggested to consider digestive problems, especially dyspepsia in patients with low back pain.

The aim of this study was to find new plant components and study antimicrobial effect of extracts of Satureja hortensis, Stachys lavandulifolia Vahl, and Mentha pullegium on methicillin resistant and methicillim susceptible Staphylococcus aureus bacteria.
In this descriptive analytic study, Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution in 96-well microplate. The level of phenolic, flavonoid, and flavonol components in the plant extracts was measured and at the end the above values were reported in mg/ml.
The lowest MIC was obtained for S. hortensis extract which was 5 mg/ml for both standard strains. S. lavandulifolia extract with 20 mg/ml MIC had the highest MIC rate for methicillin susceptible S. aureus and, besides M. pullegium extract with 10 mg/ml MIC, had the highest MIC rate for methicillin resistant S. aureus. The lowest MBC was also obtained for S. hortensis which was 5 mg/ml for methicillin susceptible and methicillin resistant S. aureus. The highest MBC for methicillin resistant S. aureus was obtained for M. pullegium extract.
S. hortensis extract contained the highest amounts of phenolic, flavonoid, and flavonol components (14.98, 25.82, 169.37 mg/ml), respectively and this extract’s amount of phenolic components was higher compared to other extracts.
S. hortensis extract contributes greatly to inhibiting the growth of methicillin susceptible and methicillin resistant S. aureus which is due to this extract phenolic components high amount.

Chronic myeloid leukemia (CML) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells، erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. The research evaluates the cytotoxic effect of hydro-alcoholic extract of M. polegium before flowering aerial organs on K562 cell line as a model of chronic myeloid leukemia. In this experimental trial، Leaves and stems of M. pulegium before flowering collected from Afoos city and extraction using maceration method. K562 cells were cultured and treated with concentrations of extract (12. 5-100 μg/ml) and different times (24،48،72 hour). Cytotoxicity of M. pulegium before flowering extract against K562 leukemia cells was estimated by the MTT test method. The absorbance was measured using an ELISA plate reader at 540 nm. Survey on data accomplished with the use of SPSS15 software and one-way ANOVA test analysis and Tukey test; and p <0. 001 was considered significant. hydro-alcoholic extract of Mentha pulegium before flowering showed the highest cytotoxic effect (IC50=50 μg/ml) and 72 hour after treatment on K562 cell line. in other words، hydro-alcoholic extract of Mentha pulegium before flowering extracts a dose and time dependent cytotoxic effect on K562 cell line Considering the cytotoxic effect of hydro-alcoholic extract of M. polegium before flowering aerial organs on K562 cells، the plant can be considered as a potential candidate for further studies on CML treatment.

Long-term consumption of many drugs followed by reduction of their effectiveness has necessitated performing research on new analgesics. Thus، the present study was conducted to evaluate the analgesic effects of mentha longifolia and morphine in mice using writhing and hot plate tests. In this experimental study، 70 male rats were divided into 7 equal groups. The groups included the control، three experimental groups receiving 400، 800، or 1600 mg/kg of mentha extract and three experimental groups which received 2، 4، or 8 mg/kg of morphine. In order to measure pain، the two acceptable tests، writhing and hot plate tests، were applied. Pain scores were measured at 0، 15، 30، 45 or 60 min after administration of algogenic stimulus. It was found that in hot plate test، only the dose of 1600mg/kg of Mentha extract after 60 minutes was significantly able to exert an analgesic effect (P<0. 05). In wrighting test، mentha extract at different doses significantly reduced the number and time of wrightes in the rats، comparable to morphine (P<0. 05). It seems that all doses of mentha extract in wrighting test have analgesic effects which indicate chronic pain inhibition of mentha hydroalcholic extract.

Pennyroyal is an aromatic herb that has been considered by various industries such as food and medicine, since it grows in different countries. Various industry`s attention to this worth product due to effective agent Pulegune (original compound essential oils of pennyroyal) that causes anti-microbial, anti-oxidant and aromatic properties. Essential oils of pennyroyal have a significantly effect on preventing the growth of several species of pathogenic and spoilage bacteria. Moreover, these oils maintain food quality by preventing the oxidation of unsaturated fatty acids and increase product shelf life. So it seems that pennyroyal is an appropriate alternative for synthetic antioxidants. On the other hand, it can be used in treatment of many conditions. Thus, pennyroyal essential oils can be a good, nature and economy alternative for food and medicine industries. The present study focuses on the characteristics of pennyroyal essential oils.