جستجوی مقالات مرتبط با کلیدواژه « کواگولاز » در نشریات گروه « پزشکی »

Staphylococcus aureus is a common pathogen in human that can be the cause of a wide range of infectious diseases including bacteremia, pneumonia, cellulitis, and osteomyelitis and skin and soft tissue infections. The coagulase enzyme is one of the most important virulence factors of this bacterium. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Coa pattern is one of the molecular base typing methods. Molecular typing plays an important role in epidemiological studies of nosocomial infection, such as methicillin-resistant Staphylococcus aureus (MRSA) infection. The PCR-RFLP Coa gene technique provides a useful preliminary method to monitor variations in MRSA populations. We were done Coa-RFLP typing according to the method of Hookey et al., with some modifications. In this cross-sectional study, one-hundred fifty isolates of S. aureus from urine and blood samples of patients that collected from educational hospitals of Imam Hossein and Al Zahra Isfahan University of Medical Sciences, Iran, from February 2018 to October 2018 were analyzed. After bacterial confirmation of isolates by Coa gene in polymerase chain reaction (PCR) technique, to perform coagulase gene typing, the repeated units encoding hypervariable regions of the coagulase gene of S. aureus were amplified by PCR. This was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. Of 150 samples, 45 isolated of S. aureus were confirmed by biochemical methods. Of previous positive samples, 36 (80%) isolates carried Coa gene. Two different genotypes of Coa gene were obtained that include bp680 fragment in 20 specimens and bp750 fragment in 16 specimens. After enzymatic digestion by AluI restriction enzyme for RFLP, four different restriction patterns were obtained that including, the 280+400 pattern in 16 specimens (44.4%), 280+470 pattern in 7 specimens (19.4%), 340+340 pattern in 6 specimens (16.6%) and 750 patterns without digestion were in 7 specimens (19.6%). Using the present experiments, it was determined that the PCR-RFLP pattern, 280+400, was the dominant pattern in the Staphylococcus aureus samples isolated in Isfahan.

Staphylococcus aureus is an important opportunistic pathogen causing a wide range of infections in human. Most clinical isolates of S.aureus are resistant to a number of antibiotics. For appropriate antimicrobial therapy, this study was conducted to determine antibacterial drug resistance patterns of S.aureus isolates obtained from different clinical samples in Rasht. the clinical isolates of S.aureus were collected from different clinical laboratories in Rasht. Thirty coagulase positive S.aureus strains were identified using biochemical tests and amplification of 23SrRNA and coa genes by polymerase chain reaction. Finally, the resistance pattern of the isolates to 16 selected antimicrobial agents was evaluated by disk diffusion method. the S.aureus isolates (75%) were resistant to methicillin and all of them were multidrug resistance. The isolates were high resistance to ampicillin (73%), amoxicillin (60%), cloxacillin (53%) and low resistance to vancomycin (7%) and gentamicin (10%). given the high prevalence of methicillin resistant, multi drug resistant and presence of vancomycin resistant S.aureus isolates in Rasht, continuously monitoring of drug resistance pattern of S.aureus isolates is recommended for having appropriate therapeutic regime.

Staphylococci are the most common causes of nosocomial infections are considered. Aminoglycosides are often used in combination with B-lactamas and glycopeptides for the treatment of endocarditis and bacteremia caused by Staphylococci. The main mechanism of aminoglycoside resistance in staphylococci is drug inactivation by cellular aminoglycoside modifying enzymes. 50 isolates of methicillin-sensitive Staphylococcus aureus and 50 isolates coagulase-negative staphylococci, were collected from various clinical specimens and were identified by standard biochemical tests. The antibiotic susceptibility pattern of isolates using the disk diffusion method and Etest for determining aminoglycoside antibiotics and the frequency of gene aac(6')-Ie-aph(2"), aph(3')-IIIa and ant(4')-Ia was determined using PCR. 26% of the samples showed resistance to at least one antibiotics.Genes aac(6')-Ie/aph(2") and aph(3')-IIIa were most abundant genes, respectively. Approximately 14% of these genes were two samples simultaneously, but in no instance a gene ant(4')-Ia were found. High prevalence of genes aac(6')-Ie-aph(2") and aph(3')-IIIa resistance genes among isloate were found. Proper antibiotic can be prescribed to prevent dissemination of resistant strains. Use phenotypic and genotypic methods simultaneously give us full information of aminoglycoside resistance

Introduction. Many investigations have, so far, carried out to determine antibiotic resistance pattern and plasmid profiles. The aim of this study was to assess coagulase positive Staphylococcus resistance to 12 selected antibiotics, using disc diffusion and to determine their possible origin, through plasmid deletion, using mitomycin C treatment. Material and Methods. Bacterial strains were isolated from clinical specimens taken from patients in Imam Khorneini (RAH) hospital and preserved at our laboratory. Being cultured in nutrient and blood agar, they were incubated for 24 hours at 37 °C. Gram staining, coagulase and DNAase monitol fermentation tests were done. After preparing inoculating suspention, disc diffusion (12 antibiotics) test was performed. Susceptible and resistant strains were also determined through their treatment with mitomycin C. Results. The results indicated that antibi otic resistance was the most against erthrornycine (89%) and mitomycin C significantly decreased the frequency of single, double and multiple resistant cases. Conclusion. These findings suggest that coagulase positive, Staphylococcus aureus is resistant, to different degree, to some selected antibiotics. Plasmids not only cause resisance to aantibiotic but also play obvious role in multiple resistance.