جستجوی مقالات مرتبط با کلیدواژه « lactate dehydrogenase » در نشریات گروه « پزشکی »

Aging is a major risk factor for developing cardiovascular diseases. Losartan is an antihypertensive drug that has shown promising effects in improving cardiovascular diseases. The aim of this study was to investigate the effect of Losartan on cardiac hypertrophy, cardiac damage markers including lactate dehydrogenase (LDH) and cardiac creatine kinase (CK-MB), and lipid profile due to D-galactose induced aging model in male rats.

The rats were randomly divided into 3 groups, with 8 rats in each group, including the control group, the aged group induced with D-galactose, and the aged group induced with D-galactose and receiving Losartan. Cardiac hypertrophy was measured by histological examination and measurement of heart weight and body weight. Also, serum lipid profile and heart damage markers were measured.

The findings showed that aging induced by D-galactose causes cardiac hypertrophy, hyperlipidemia and increased serum levels of heart damage markers. In addition, treatment with Losartan decreased cardiac hypertrophy, decreased cholesterol, decreased low density lipoprotein (LDL), and decreased cardiac injury markers in aged rats.

The results of the present study show that Losartan is effective in improving cardiac hypertrophy and reducing heart damage caused by D-galactose-induced aging, which is partially done by improving lipid profile.

Several studies have investigated the effect of creatine supplementation on indirect markers of muscular damage and sports performance of athletes. However, pooled data from several studies suggest that the benefits of creatine on recovery of athletes are limited. The aim of this study was to investigate the effect of creatine supplementation on post-exercise recovery and muscle injury factors in healthy subjects.

A systematic search of articles published in English and Persian databases until November 2023 was conducted to investigate the effect of creatine supplementation on muscle injury markers creatine kinase (CK) and lactate dehydrogenase (LDH) and muscle soreness in healthy subjects. Weighted mean difference (WMD) and standardized mean difference (SMD) and 95% confidence interval were calculated using random effect model.

A total of 20 studies including 458 subjects were included in the present meta-analysis. The results showed that creatine did not cause a significant change in muscle soreness in any of 24, 48, 72, and 96 hours after exercise compared to the control group. Also, creatine did not cause a significant change in CK in any time spectrum compared to the control group. In addition, creatine did not cause a significant change in LDH in different time ranges after exercise compared to the control group in athletes and non-athletes.

The results of the present study show that creatine supplementation is not effective in reducing muscle soreness and muscle injury factors after exercise.

Coronavirus disease 2019 (COVID-19) can lead to organ failure by the occurrence of mechanisms such as increased thrombosis and, subsequently, increased lactate dehydrogenase (LDH). This study was conducted to determine LDH serum levels in COVID-19 patients and the factors affecting their mortality.

This descriptive-analytical study was conducted on 212 patients (57 males and 155 females) with COVID-19 with a mean age of 49.19±10.6 referring to Imam Ali Hospital in Chabahar, Iran during 2021. After obtaining patients’ informed consent and demographic information, the heparinized peripheral blood sample was taken from them. The LHD levels were determined using an autoanalyzer.

Twenty-nine (13.67%) patients died. The mean LDH serum level of 29 deceased patients (708.420±96.25 U/L) was not statistically significant compared to survivors (640.360±96.80 U/L in 183). The comparison between the surviving and deceased groups showed that 25% of the deceased patients were hospitalized in the intensive care unit (ICU), and 90.90% of the survivors were hospitalized in the internal ward (P<0.05). All the deceased and 85.85% of the survivors were 40 years old and above, and this difference was not statistically significant. Furthermore, 24.56% of the deceased were male, 90.32% of the survivors were female (P<0.05), 22.72% of the deceased had a university education, and 88.69% of the survivors had a diploma or under-diploma education (P<0.05), and 71.42% of the deceased patients had thin, and 91.37% of the survivors were overweight (P<0.05).

There was no difference in the LDH serum levels of the COVID-19 survivors and deceased. The age of 40 years and above, lean and morbidly obese body mass indices, male gender, and the need for hospitalization in the ICU were determined as risk factors.

Background and

Today, cancer is considered as the second cause of death in the world. Often, the treatment of all types of cancer is very complicated. The discovery of new anti-cancer drugs with high effectiveness, low toxicity, the ability to select normal cells from cancerous cells, and low cost is one of the concerns of the world's pharmaceutical communities. Nowadays, the use of natural compounds based on their synergistic effects has opened a new therapeutic horizon in the management of different types of cancers. The aim of this study was to investigate the synergistic effects of peiminine and hyperthermia therapy on the induction of apoptosis in MCF-7 cell line.

In the present experimental-laboratory study, after culturing MCF-7 cells in 96-well plates, they were treated with peiminine (5.12 µg/ml for 24 h) and hyperthermia (410C for 1 h) independently and simultaneously. Then the cell viability rate, apoptosis percentage, ROS production rate, LDH release rate, and caspase 8 and 9 activity level were measured.

The results of the present study showed that peiminine and hyperthermia therapy caused a significant decrease in cell viability as well as a significant increase in the percentage of apoptosis, ROS production, and LDH release compared with the control group. By measuring the activity of caspase 8 and 9, it was determined that peiminine induced apoptosis through both mitochondrial and extracellular pathways. It was also found that peiminine and hyperthermia therapy, in combination, had synergistic antiproliferative effects.

According to the results of the present study, it seems that peiminine, as a natural product, when used in combination with hyperthermia therapy has synergistic antiproliferative and apoptosis-inducing effects. This suggests that it can be used as a complementary method in cancer treatment.

Objective:

 The use of amino acid supplements among athletes for reducing muscle injuries has become more popular. This study aims to examine the effect of branched-chain amino acids (BCAA) consumption before exhaustive exercise along with one month vitamin E supplementation on lactate dehydrogenase (LDH) and creatine kinase (CK) levels of active females.  

Methods :

In this study, 32 active female students of Razi University in Kermanshah, Iran aged 18-23 years were selected and, after obtaining the informed consent form them, were randomly divided into four groups of placebo (n=8), Vitamin E (n=8), BCAA (n=8), and Vitamin E+BCAA (n=). Vitamin E was consumed as 400 IU daily for a month and BCAA with lemon juice was consumed 2.5 hours before exercise four times with a 30-min interval. The exercise program included 30 minutes of cycling on an ergometer with 50% of the aerobic capacity, and immediately followed by cycling with 75% of the aerobic capacity until the exhaustion. Sampling was done immediately and 48 hours after the exercise, and data were analyzed using Shapiro-Wilk test, repeated measures ANOVA, Bonferroni test, and Pearson correlation test in SPSS software, version 22.

Results:

 None of the supplements had a significant effect on levels of LDH and CK immediately after the exercise (P>0.05), but 48 hours after the exercise, the mean levels of LDH and CK decreased in BCAA group  (CK: 199.4±11.00, LDH: 213.2±23.44) and Vitamin E+BCAA (CK: 188.3±3.20, LDH: 208.3±40.12) compared to Vitamin E and placebo groups (P = 0.001). Their lowest levels was observed in the Vitamin E+BCAA group which was negatively correlated to plasma leucine and isoleucine levels (P = 0.001). 

Conclusion :

Although BCAA consumption alone before exhaustive exercise can reduce muscular damage indicators in active females, it seems that leucine and isoleucine along with Vitamin E supplementation is more effective in reducing muscular damage.

Doxorubicin (Dox) is a very effective drug for the treatment of a wide range of cancers. However, in healthy tissues, especially cardiac tissue, it can lead to cytotoxicity. This study aims to determine the effect of 8 weeks of high intensity interval training (HIIT) and crocin consumption on serum markers of Dox-induced cardiotoxicity in male rats

In this study, 32 male Wistar rats were randomly divided into four groups: healthy control, doxorubicin, doxorubicin + HIIT,  and doxorubicin + crocin + HIIT. The HIIT program was performed for 8 weeks, five days a week, with two 2-min intervals and 80% intensity in the first week and eight 2-minute intervals and 90% intensity of maximum speed in the final weeks. The last group received 10 mg/kg body weight of crocin by gavage for 8 weeks. Dox was injected subcutaneously seven times at a dose of 2 mg/kg. Forty-eight hours after the last training session and after anesthesia, blood samples were taken directly from the left ventricle; after separating serum from plasma, the levels of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) were measured. Data were analyzed using independent t-test and one-way analysis of variance. The significance level was set at 0.05.

The Dox injection led to a significant increase in LDH and CK-MB levels in rats compared to the healthy control group (P=0.001). However, 8 weeks of HIIT significantly reduced the serum levels of LDH (P=0.001) and CK-MB (P=0.017) compared to the Dox group. In addition, 8 weeks of HIIT plus crocin consumption significantly reduced the LDH (P=0.001) and CK-MB (P=0.001) levels compared to the Dox group. There was no significant difference between the effects of HIIT alone and HIIT plus crocin consumption on the serum levels of LDH (P=0.087) and CK-MB (P=0.877) in rats received doxorubicin.

It seems that HIIT alone and in combination with crocin consumption can reduce the changes in serum levels of LDH and CK-MB induced by doxorubicin.

Intense physical activity, despite its various benefits for general health, can cause possible damage to various tissues of the body due to increasing oxidative stress through increasing production of reactive species. Muscle bruising and pain is a common experience that occurs after performing unusual and intense activities, especially after resistance and extroverted activities. Delayed muscle spasm is associated with the release of creatine kinase, lactate dehydrogenase, and aspartate aminotransferase, and can be measured by the release of these enzymes into the blood. Numerous studies have examined the effect of resistance trainings on muscle injury indices that creatine kinase and lactate dehydrogenase, spartate aminotransferase, alanine aminotransferase and myoglobin indices increased significantly after exercise. In another study, they did not see any significant changes in CK and LDH muscle injury indices after 3 hours of cycling. Therefore, due to the injuries, researchers have drawn attention to the effects of resistance exercise on muscle injury indices. Although resistance training improves performance, it does not prevent the release of inflammatory and traumatic factors, so these factors can cause acute sports injuries, which will be especially important at a young age. Nowadays, special attention is paid to the use of various methods of resistance training, the most prominent of which is TRX or the same resistance training exercises suspended by body weight. Therefore, considering the different effects of sports activities, especially strength training on different parts of the body and the possibility of injury in these exercises, and considering the differences in the type of resistance training programs such as TRX and circular resistance, it is important to pay attention to the effects of this type of training and muscle injury indices. Also due to the contradictory results about the effect of exercise, especially the effect of resistance activity on muscle injury indices and also the lack of sufficient research on the long-term effect of TRX training on muscle injury indices and especially insufficient research on the comparison of resistance training and TRX, the researcher decided to compare 8 weeks of traditional resistance training and TRX on the indicators of muscle injury in inactive women.

In this semi-experimental study, (28) healthy inactive girls with a mean age of 21.00 38 1.38 years were randomly divided into three groups: traditional resistance training (9), TRX training (9) and control (10). The experimental groups performed the training protocol three times a week for 8 weeks with an intensity of 65 to 80%. Blood samples were taken before training and 48 hours after the last training session and creatine kinase and lactate dehydrogenase levels were measured. Data were statistically analyzed using Shapiro-Wilk, dependent t-test and one-way analysis of variance.

The results showed that traditional resistance training and TRX both cause significant changes in LDH levels, while there was no significant difference in CPK variable from pre-test to post-test in all three groups. Also, in examining the differences between groups, no significant difference was observed between the three study groups at CPK levels. While there was a significant difference in the amount of lactate dehydrogenase between the three groups (p <0.05). According to the results of Table 3, there was a significant difference in the levels of lactate dehydrogenase between the resistance group and the TRX group (p = 0.0001), the control group with the TRX group (p = 0.0001) and the control group with the resistance group (p = 0.012) was observed.

The presence of CK and LDH in the blood is an indirect sign of muscle damage as well as the diagnosis of medical conditions such as heart attack, muscular dystrophy and brain diseases. Cellular disorders, especially muscle cells, can cause CK and LDH to leak from cells into the blood serum. The findings of the present study showed that although there was a difference in the means of the three groups in both variables of creatine kinase and LDH, but this difference was significant only for LDH (P <0.05). The results of this study also showed that there is no significant difference in creatine kinase index between the two types of traditional resistance training and TRX after eight weeks. These factors affect the amount of response and the time period of secretion along with the injury. In general, the researchers state that intense resistance activities due to increased mechanical-metabolic pressure on the fibers lead to rupture of fibers, fluidization of z-plates, rupture of sarcolemma, displacement of intracellular organs, instability of plasma membranes and increasing secretion of intracellular proteins, after performing the resistant and intense activity. In fact, fatigue of muscle fibers following exhaustive activities can lead to increasing permeability of cell membranes to intracellular free calcium ions and dysfunction of sodium-potassium pumps, causing instability of cell membranes and activation of proteases and intracellular lipases. There is a close relationship between the release of phospholipases and creatine kinase due to the activity of intracellular proteolytic enzymes stimulated by calcium in the isolated muscle of mammals. The results of this study on LDH showed that the LDH of the experimental groups was significantly lower than the control group. Lactate dehydrogenase is one of the enzymes involved in the anaerobic pathway of ATP production. The secretion of lactate from the training muscle and the activity of the muscle pump indicate the role of continuous exercise training on the rate of cell damages. It has also been shown that trained people have less enzymatic release into the bloodstream than people who did not, which may be due to the adaptation of trained people to the physical activity and exercise; because the skeletal muscles of athletes and trained people have the highest amount of ATP, which is used during exercise and physical activity to maintain the integrity of the cell membrane, and as a result, reduces the leakage of enzymes into the blood. In any case, it seems that as a result of running consecutive weeks of regular exercise programs, intramuscular adaptation may be established, and the nature of resistance training programs causes adaptation to occur at all levels, including intramuscular and better blood supply, which reduces the activity level of CK and LDH enzymes as indicators of muscle and cell damage; therefore, it seems that the use of appropriate methods of training reduces the disruption of biochemical changes. Therefore, according to the findings of the present study, it can be stated that the implementation of TRX training program and traditional resistance both can significantly reduce the rate of LDH cell damage index and no change in CK, and there is no difference between this type of training on the indicators muscle damage in young women, so both types of exercise can have beneficial effects on muscle injury indices.

Introduction: Cardiac biomarkers play an important role in predicting heart damage. The aim of this study was to investigate The effect of aerobic maximal workload exercises on Galactin-3, plasma Creatine Cinase-MB and lactate dehydrogenase concentration of Iranian Soccer premier league players. Methods: The statistical population consisted of players of the Iranian Football Premier League in the season 1399-1400. 20 football players were selected as available samples. Evaluation of aerobic activity was incremental to maximal with the implementation of the Bruce protocol. Blood samples were taken before, immediately and 24 hours after the end of the protocol. To test the hypotheses and compare the dependent variables in different groups, analysis of variance in repeated measures and Bonferroni test were used to compare between blood sampling steps. Data analysis was performed using SPSS25 software. Results: There was no significant difference in the amount of Galactin-3 between the pre-test and post-test stages and also between the post-test and 24 hours after the test. There was a significant difference in Creatine Kinase-MB and Lactate Dehydrogenase levels between pre-test and post-test stages as well as between pre-test and 24 hours after test. Conclusion: The merobic maximal workload exercises alters serum Galactin-3 levels in Premier League players, possibly due to inflammatory and anti-inflammatory effects during exercise and post-exercise recovery. Also, based on the findings of the present study, increasing aerobic exercise to the maximum caused a significant difference in levels of creatine kinase MB and lactate dehydrogenase in the post-test and 24 hours after the test.