جستجوی مقالات مرتبط با کلیدواژه "seminiferous tubules" در نشریات گروه "پزشکی"
-
مقدمه
هدف از مطالعه ی حاضر، بررسی ارتباط میزان بیان فاکتور مهم رونویسی POU5F1 در سلول های بنیادی اسپرماتوگونی با سن و ارایه ی الگوهای بیانی این فاکتور در لوله ی اسپرم ساز موش می باشد.
روش هادر مطالعه ی حاضر پس از استخراج بیضه ی موش های نوزاد دو هفته ای و بالغ 16 هفته ای که از موسسه ی پاستور ایران خریداری شد، سلول های بنیادی اسپرماتوگونی جدا سازی گردید؛ پس از کشت سلول ها در محیط کشت StemPro-34 medium حاوی فاکتورهای رشد اختصاصی کشت داده شدند. همینطور بافت برش داده شده پس از انجام مراحل فیکس کردن و آماده سازی، با آنتی بادی های اولیه و ثانویه انکوبه شدند و بررسی ایمنوهیستوشیمیایی با میکروسکوپ کونفوکال انجام گردید. همچنین آنالیز real-time PCR برای بررسی کمی میزان بیان فاکتور رونویسی POU5F1 استفاده شد.
یافته هادر حالی که در آنالیز ایمنوهیستوشیمیایی، بیان بالای فاکتور رونویسی POU5F1در بیضه ی نوزاد مشاهده شد، تعداد سلول های POU5F1 مثبت در لوله های اسپرم ساز بیضه ی بالغ بیشتر از نوزاد بود. همینطور تجزیه و تحلیل real-time PCR نشان داد که میزان بیان ژن POU5F1 در SSC های نوزاد به طور معنی داری (0/05 P <) بالاتر از SSCهای 16 هفته ای بود.
نتیجه گیرینتایج حاصل می تواند پایه ابعاد جدیدی در ارتباط فاکتورهای اپی ژنتیکی با قدرت تمایزی سلول های بنیادی را آشکار سازد که باید در پژوهش های تمایز آزمایشگاهی سلول های بنیادی اسپرماتوگونی به سلول های اسپرم مورد توجه قرار گیرد.
کلید واژگان: بیضه, لوله های اسپرم ساز, سلول های زایا, فاکتور رونویسی, POU5F1BackgroundThe purpose of this study is to investigate the relationship between the expression level of the important transcription factor POU5F1 in spermatogonial stem cells with age and to present the expression patterns of this factor in the mouse spermatogenic tube.
MethodsIn the present study, spermatogonial stem cells were isolated after extracting the testicles of two-week-old and sixteen-week-old adult mice purchased from the Pasteur Institute of Iran. The cells were cultured in a StemPro-34 medium containing specific growth factors. Also, after fixing and preparing the tissue, they were incubated with primary and secondary antibodies, and an immunohistochemical examination was done with a confocal microscope. Then, real-time PCR analysis was used to quantitatively investigate the expression of the POU5F1 transcription factor.
FindingsWhile in the immunohistochemical analysis, high expression of the POU5F1 transcription factor was observed in the infant's testis, the number of POU5F1 positive cells in the seminiferous tubules of the adult testis was higher than that of the infant. Also, real-time PCR analysis showed that the level of POU5F1 gene expression in newborn SSCs was significantly (P < 0.05) higher than 16-week-old SSCs.
ConclusionThe results can reveal the foundation of new dimensions in the relationship between epigenetic factors and the differentiation power of stem cells, which should be considered in research on the differentiation of spermatogonial stem cells into sperm cells.
Keywords: Testis, seminiferous tubules, Germ cells, Transcription factor, POU5F1 -
The aim of this study was to investigate the effects of long-term administration of clenbuterol on the histomorphometry and histopathology of testicular tissue in mice. 30 male NMRI mice were divided into three groups including the control group that received (0.1 ml of normal saline) the second group, (2.5 mg /kg clenbuterol) and the third group (8 mg/kg clenbuterol) that received for 30 consecutive days. At the days 3 and 30 from the beginning of the study, five mice were randomly sacrificed from each experimental group. The testicular tissue was removed from the right testis and fixed in the Bouin's solution for histomorphometric and pathological examinations. Degenerative lesions including distortion of the germ cells, reducing their number, and presence of vacuoles within the seminiferous epithelium were demonstrated in the clenbuterol treated groups. At the present study, administration of clenbuterol decreased spermatogenesis parameters including meiosis index, Johansen score, spermatogenesis percentage, seminiferous tubules diameter and epithelium height of seminiferous tubules both in the day 3 in low and high dose of clenbuterol with more severity in high dose. On the 30th day, testicular degenerative changes due to clenbuterol were more in the low dose of rather than high dose. According to the obtained results, it can be concluded that administration of clenbuterol may has potentially negative affect on the sperm production and fertility in male mice.
Keywords: Beta-2 agonist, Testes, Infertility, Degeneration, Seminiferous tubules, Spermatogenesis -
Background
Sox2 (SRY box2) is an essential transcription factor that plays a vital role in spermatogenesis and regulates the genes in this process. Sox2 is important for pluripotency, self-renewal, and even spermatogonial stem cell differentiation. This gene is found in pluripotent and specialized cells, and it is involved in their biological activities.
MethodsProtein-protein interaction (PPI) network analysis was performed during spermatogenesis using NCBI, STRING, and Cytoscape databases. Then, after isolating spermatogonial stem cells from 6 C57BL/6 mice, mouse embryonic stem cells and ES-like cells were prepared. In the following, Sox2 expression was examined in differentiated and undifferentiated spermatogonia by immunohistochemistry (IMH), immunocytochemistry (ICC), and Fluidigm PCR (polymerase chain reaction). Finally, the results were compared using the Kruskal-Wallis and Dunn tests at the significance level of p<0.05.
ResultsThe results of this experiment showed that contrary to expectations, Sox2 has cytoplasmic expression in undifferentiated cells and nuclear expression in differentiated cells in in vitro conditions. In addition, the expression of Sox2 increased during differentiation. Fluidigm PCR showed a significantly higher expression of Sox2 (p<0.05) in differentiated compared to undifferentiated spermatogonia. Sox2 has an interaction with other genes during spermatogenesis such as Oct4, Nanog, Klf4, Stra8, Smad1, Tcf3, and Osm.
ConclusionSox2, which is known as a pluripotency marker, has a vital role in spermatogenesis and could be a differential marker. Sox2 has strong connections with other genes such as Oct4, Nanog, Klf4, Tcf3, Osm, Stra8, Lim2, Smad1, Gdnf, and Kit.
Keywords: Adult germline stem cells, Cell differentiation, Seminiferous tubules, Sox2 protein, Transcription factors -
مقدمه
مت آمفتامین ماده محرک سیستم عصبی مرکزی است که معمولا سوء مصرف می شود. هدف مطالعه حاضر تعیین اثرات مت آمفتامین بر قطر و ضخامت لوله های سمینی فر گناد موش های صحرایی نر است.
روش کاردر این مطالعه از 20 سر موش صحرایی نر بالغ با وزن 200 تا 220 گرم استفاده شد. موش ها به دو گروه آزمایش و کنترل هر گروه 10 موش تقسیم شدند. در گروه آزمایش موش های صحرایی سه روز اول 2 میلی گرم و چهار روز بعد، 5 میلی گرم مت آمفتامین به ازای هر کیلوگرم وزن بدن به صورت داخل صفاقی دریافت کردند. گروه کنترل نیز 5/0 میلی لیتر سرم فیزیولوژی به صورت داخل صفاقی به مدت یک هفته به ازای هر موش دریافت کردند. بعد از گذشت 24 ساعت از آخرین تزریق، موش های صحرایی کشته و گناد اصلی از اسکروتوم خارج شد. بعد از پاساژ بافتی و رنگ آمیزی با هماتوکسیلین-ایوزین، با میکروسکوپ نوری بررسی شدند. نتایج با استفاده از آزمون تی مستقل تحلیل شد. مقدار 05/0 P< برای تعیین سطح معنی داری بین گروه ها در نظر گرفته شد.
یافته ها:
نتایج پژوهش نشان داد مصرف مت آمفتامین باعث کاهش معنادار میانگین قطر و ضخامت اپی تلیوم لوله های سمینی فر در موش های صحرایی می شود.
نتیجه گیریبا توجه به نتایج به دست آمده از این مطالعه، مت آمفتامین با دژنراسیون و آتروفی لوله های اسپرم ساز یک ترکیب سمی برای گناد اصلی موش های صحرایی نر است
کلید واژگان: تستوسترون, گناد اصلی, لوله های سمینی فر, مت آمفتامین, موش صحرایی نرIntroductionMethamphetamine is a stimulant of the central nervous system that is usually misused. This study aimed to determine the effects of methamphetamine on the diameter and thickness of seminiferous tubules of adult male rats’ main gonad.
MethodTwenty adult male rats weighted 200 to 220 gr were used in the current study. They were divided into study and control groups, each consisting of 10 rats. The study group received 2 mg per body weight of methamphetamine in the first three days and 5 mg in the next four days in peritoneum. The control group, on the other hand, received 0.5 ml per rat of physiologic serum in the the peritoneum. Twenty-four hours after the last injection, the rats were killed, and the main gonad was taken out of the scrotum. After tissue passage and dying with hematoxylin and eosin, the main gonad was observed with an optic microscope, and the results were analyzed using an independent t-test. A value of P<0.05 was considered to determine the level of significance between the two groups.
ResultsThe findings showed that using methamphetamine significantly decreases the average diameter and thickness of the epithelium of seminiferous tubules of rats. It was also found that the weight of rats and gonads decreased, but it was not statistically significant.
ConclusionThe results of this study show that methamphetamine is a poisonous compound for the main gonad of male rats due to the degeneration and atrophy of seminiferous tubules
Keywords: Main gonad, Male rat, Methamphetamine, Seminiferous tubules, Testosterone -
Background
Neem leaves (Azadirachta indica L.) have been used for many therapeutic purposes and medicinal applications. The extract of this plant has been used in both male and female genders as a traditional agent to prevent early pregnancy. In this study, the effect of this extract was investigated histologically and morphometrically on the germinal epithelia of the seminiferous tubules, epididymis and secretory epithelia of rats’ prostate glands.
MethodsTwenty male albino rats were divided into four groups of five each and administered the extract at a concentration of zero, 100, 200 or 400 mg/kg of the body weight for 50 consecutive days. These rats were sacrificed and the male reproductive organs were removed, weighed and processed for routine histological examinations. The micrographs were analyzed and the structural changes in the epithelial lining and morphometric analyses were recorded, which included measuring the epithelial thickness in the seminiferous tubules, epididymis and secretory prostatic epithelia.
ResultsThe extract was found to reduce the rats’ weight; decreased both the weight and dimension of the testes; reduced the number of germinal epithelial lining cells in the seminiferous tubules of the testes, the epididymal and prostatic secretory epithelial cells.
ConclusionThe histological alterations were most significant in response to the treatment with the extract at 200 mg/kg of the rats with the greatest damages observed in the epithelial lining. The deleterious effects of the extract were found to be dose-dependent and this corroborates the use of this extract as a contraceptive in animal models, and potentially in humans.
Keywords: Epididymis, Epithelia, Morphometry, Prostate gland, Seminiferous tubules -
Purpose
Oilseeds and their related products are known to have various bioactive and health-promoting ingredients. In this research, we investigated the effects of phytosterols and fatty acids of Pistacia vera on spermatogenesis process and testis histological changes in Wistar male rats for the first time.
Materials and MethodsA total number of 64 adult male Wistar rats were divided randomly into eight groups including one control group, and seven test groups. Test groups received phytosterols, fatty acids, and pistachio oil orally for 30 days. Then, LH, FSH, and serum testosterone levels were determined. Also, the spermatogenesis process and changes in testicular tissue in rats were investigated.
ResultsThe results of this research suggest that phytosterols in doses of 10 and 50 mg/kg reduce the spermatogenesis process. Fatty acid in a low dose of 10 mg/kg increases spermatogenesis, but when a high dose of 50 mg/kg was used, it harmed the spermatogenesis process. When low levels of phytosterols and fatty acids are used simultaneously in dose 5 mg/kg, improvement in spermatogenesis process is observed but when these were used together in the dose of 25 mg/kg, the spermatogenesis process was disrupted. Using pistachio oil alone also improved spermatogenesis process.
ConclusionIt seems that phytosterols reduce spermatogenesis at high and low doses, while fatty acids increase spermatogenesis when used in low doses and reduce this process when used in high doses. The use of fatty acids extracted from pistachios to treat infertility in men seems hopeful.
Keywords: Infertility, Nut, Spermatogonia, Spermatid, Seminiferous tubules, sertoli cells, epithelial layer thickness -
Background
Spermatogenesis system is one of the most radiosensitive organs in the body. A usual therapeutic dose of radiation such as the conventional 2 Gy in each fraction of radiotherapy and lower doses seen in diagnostic radiology or a radiation disaster affect the process of spermatogenesis potently. Selenium and zinc are two important elements playing key roles in the development of sperms and also have radioprotective effects.
ObjectiveIn this study aims to evaluate the radioprotective effect of zinc and selenium against radiation-induced mice testis injury.
Material and MethodsIn this experimental study, 30 mice were divided equally into 6 groups, including control selenium treated, zinc treated, radiation, radiation + selenium, radiation + zinc. Treatments started from 2 days before irradiation with 2 Gy cobalt-60 gamma rays. After 37 days, all mice were killed for histopathological evaluations.
ResultsResults showed that exposure to radiation caused a potent effect on spermatogenesis system. Treatment with selenium reversed these radiation effects potently, while zinc had some limited protective effects. Zinc treatment itself caused a detrimental effect on epididymis and, in combination with radiation, it leads to more damage to seminiferous tubules.
ConclusionIn contrast to previous studies that proposed zinc to protect spermatogenesis against various toxic agents, results of this study showed that although zinc may protect from some parameters, it potentiates radiation damage on seminiferous tubules and has a detrimental effect on the epididymis. By contrast, zinc and selenium could alleviate radiation-induced toxicity on the most of the evaluated parameters.
Keywords: Radiation, Spermatogenesis, zinc, Selenium, Seminiferous Tubules, Epididymis -
International Journal of Reproductive BioMedicine، سال هجدهم شماره 7 (پیاپی 126، July 2020)، صص 531 -538مقدمه
تامسولوزین یک عامل مهار کننده گیرنده های آلفا آدرنرژیک است که برای از بین بردن علایم بالینی و درمان احتباس ادراری حاد استفاده می شود.
هدفهدف از این مطالعه بررسی اثرات تامسولوزین بر محور اندوکرین و بافت بیضه در موش های صحرایی نر بالغ است.
مواد و روش هادر این مطالعه تجربی 30 سر موش صحرایی نر بالغ نژاد ویستار (وزن 250 تا 300 گرم) به سه گروه تقسیم شدند: 1-شاهد (آب مقطر)، 2- آزمایش 1 (تامسولوزین 2/0 میلی گرم بر کیلوگرم در روز) و 3 - آزمایش 2 (دریافت تامسولوزین 4/0 میلی گرم بر کیلوگرم در روز)، دریافت دارو از طریق گاواژ خوراکی به مدت 28 روز انجام شد. سطح هورمون های سرمی و هیستوپاتولوژی بیضه در پایان آزمایش مورد ارزیابی قرار گرفت.
نتایجوزن بیضه در این مطالعه در گروه آزمایشی به طور معنی داری نسبت به گروه کنترل کاهش یافت. کاهش معنی داری در وزن بیضه (004/0 p=) و تعداد سلول های لیدیگ (012/0p=) در گروه های تحت درمان با تامسولوزین دیده شد. تامسولوزین موجب بهبود پروفایل هورمون ها را در گروه های آزمایشی شد. همچنین تامسولوسین با دوز بالا به طور معنی داری تعداد سلول های لیدیگ، اسپرماتوگونی، ضخامت لایه ژرمینال و قطر لوله های منی ساز را تغییر داد.
نتیجه گیریبا توجه به نتایج این مطالعه، استفاده از تامسولوسین، احتمالا با تاثیر بر سیستم آدرنرژیک، باعث کاهش غلظت تستوسترون می شود و تاثیرات مخربی بر فعالیت پرولیفراتیو سلول های زایا دارد. بنابراین، پیشنهاد می شود برای کاهش اثرات جانبی، بهتر است تامسولوزین با دارویی دیگر بصورت ترکیبی تجویز گردد.
کلید واژگان: تامسولوزین, لوله منی ساز, هیستوپاتولوژی, موش صحرایی, بیضهBackgroundTamsulosin is an inhibitory factor of alpha-adrenergic receptors that is used for relieving of the clinical symptoms and management of acute urinary retention.
ObjectiveThe aim of this study was to evaluate the effects of tamsulosin on the endocrine axis and testicular tissue in adult male rats.
Materials and MethodsIn this experimental study, 30 adult male Wistar rats (weighing 250-300 gr) were divided into three groups: 1) control (received distilled water), 2) experimental 1 (received 0.2 mg/kg/day tamsulosin) and 3) experimental 2 (received 0.4 mg/kg/day tamsulosin) through oral gavage for 28 days. Serum hormones level and testicular histopathology were evaluated at the end of the experiment.
ResultsIn this study, the testicular weight decreased significantly in the experimental groups compared to the control group. A significant decrease was seen in testicular weight (p = 0.004) and the number of Leydig cells in tamsulosin-treated groups (p = 0.012). Tamsulosin improved the hormone profile in experimental groups. Also, higher dose of tamsulosin significantly changed the number of Leydig, spermatogonia cells, the thickness of germinal layer, and the diameter of the seminiferous tubules.
ConclusionResults showed that using tamsulosin, possibly reduces the testosterone concentration through adrenergic axis system and in turn has destructive effects on proliferative activity of germ cells.
Keywords: Tamsulosin, Seminiferous tubules, Histopathology, Rat, Testis -
Objective(s)Pyriproxyfen as an insect growth regulator is widely used globally for pest management. There are reports on adverse effects of insecticides such as organ toxicity, endocrine disruptions, and teratogenicity in animals and humans. We aimed to investigate reproductive toxicity of pyriproxyfen in adult male mice.Materials and Methods48 male Swiss albino mice were divided into eight groups and received the different 1200, 600, 320, 200, 100, 40, 20, 0 mg/kg/day doses orally, and body weights were accessed for 28 consecutive days. In the end, mice were sacrificed, testes were dissected and weighed. Probable testicular tissue alterations were examined by histopathological studies. In addition, the diameter of seminiferous tubules and Leydig cells distribution were assessed in all experimental and control groups.ResultsPyriproxyfen treatment caused significant (P<0.05) reduction in body and organ weights in mice. However, the shrinkage and displacement of seminiferous tubules, reduced lumen diameter, and vacuolization occurred in seminiferous tubules in higher doses exposed animals in comparison to controls. The relative testis weights, mean diameter of seminiferous tubules, and Leydig cells distribution remained unchanged at low doses.ConclusionThese findings reveal that pyriproxyfen caused reduction in body weight gain as well as damage to the testicular architecture in mice and thus may potentially interfere with spermatogenesis. Findings in an outbred strain of mice can be extrapolated fairly reliably to the human model. The chemical can thus be further exploited to study its effects on impairment of fertility and as an endocrine disruptor.Keywords: Endocrine disruptor, Histopathology, Leydig cells, Pyriproxyfen, Reproductive Toxicity, Seminiferous tubules
-
Background
Diabetes can increase the generation of free radicals and can be harmfully effective in spermatogenesis. Crocin is a carotenoid and is accountable for the red color of saffron. Crocin has shown numerous pharmacological actions such as antioxidant roles and radical scavenging. The aim of this study was to determine the effects of crocin on sperm parameters and the diameter of seminiferous tubules in diabetic rats.
Materials and MethodsIn this experimental study, diabetic rats were induced by Streptozotocin (STZ) (60 mg/kg). Sixty-four rats were equally divided into the following eight groups; (1) normal control group, (2–4) crocin groups, receiving various doses of crocin (12.5, 25, and 50 mg/kg), (5) diabetic control group, and (6–8) diabetic groups, receiving STZ plus crocin (12.5, 25, and 50 mg/kg) injected intraperitoneally once a day for 28 consecutive days. The sperm count, motility, morphology, viability, spermatogenesis index (SI), and the diameter of seminiferous tubules were examined and compared.
ResultsThe results demonstrated that count, motility, viability, normal sperm morphology, SI, and the diameter of seminiferous tubules decreased significantly in the diabetic control group compared to the normal control group (P < 0.05). However, in the diabetic groups, count, motility, normal morphology, viability, SI, and the diameter of seminiferous tubules enhanced significantly in total doses compared to those of the diabetic control group (P < 0.05).
ConclusionIt seems that, as a strong antioxidant, crocin could compensate for the toxicity induced through STZ and raise the quality of some sperm parameters.
Keywords: Crocin, diabetes, seminiferous tubules, sperm parameters -
ObjectiveThe incidence rate of testicular cancer among young males is high. Co-administration of bleomycin, etoposide and cisplatin (BEP) has increased survival rate of patients with testicular cancer. Although BEP is one of the most effective treatment for testicular cancer, but it severely affects the reproductive system that ultimately leads to infertility. In addition to its antioxidant activity, zinc has an important role in progression of spermiogenesis. This study aimed to evaluate the effect of zinc on sperm parameters, chromatin condensation and testicular structure after BEP treatment.Materials And MethodsIn this experimental study, 40 male rats were divided into 4 groups (control, BEP, BEP zinc and zinc) and examined for 2 spermatogenesis periods (i.e. 18 weeks). The rats in BEP and BEP zinc group were treated with BEP at appropriate doses (0.75, 7.5, and 1.5 mg/kg) for three cycles of three weeks. Zinc at a dose of 10 mg/kg/day was administered to BEP zinc and zinc groups. After 18 weeks, we assessed sperm parameters, and excessive histone in sperm chromatin using aniline blue staining, as well as testicular structure and germ line cells using periodic acid-Schiff staining.ResultsAfter BEP treatment, significant decreases were observed in normal sperm morphology, motility, and concentration, as well as alterations in rat sperm chromatin condensation and testicular tissue (PConclusionZinc administration after chemotherapy with BEP in testicular cancer might be potentially useful in declining the off target consequence associated with oxidative stress.Keywords: Chemotherapy, Chromatin, Seminiferous Tubules, Spermatozoa, Zinc
-
IntroductionCisplatin is a platinum-based drug widely used for the treatment of different cancers. Cell surface glycoconjugates play an important role in cell-cell interactions. The present investigation was carried out to study the toxic effects of double dose injection of cisplatin on cell surface glycoconjugates in rat as an experimental model.MethodsIn this experimental study, 45 adult male Sprague Dawley rats were used. Experimental group E1 and experimental group E2 received two repeated dose of 2.5 mg/kg and 5 mg/kg of cisplatin, respectively in the beginning of the first and fifth week of the experiment. After 8 weeks of injection, rats were killed. Tissue samples were removed and prepared sections were stained with H&E, PNA (Peanut agglutinin), and UEA (Ulex europaeus agglutinin) methods. Prepared microscopic slides were utilized for both histopathological and morphometrical studies. The obtained data were analyzed by ANOVA and Tukey tests using SPSS.ResultsCisplatin administration induced a significant decrease in internal and external diameters of seminiferous tubules in the experimental groups compared to the control one (PConclusionCisplatin induces a dose-dependent morphological changes of germinal epithelium and extensive changes in distribution pattern of fucose- and Gal/GalNac-containing glycoconjugates in seminiferous epithelium in rats.Keywords: Cisplatin, Seminiferous tubules, Glycoconjugates, Spermatogenesis, Rat
-
مجله دانشگاه علوم پزشکی شهید صدوقی یزد، سال بیست و سوم شماره 12 (پیاپی 113، اسفند 1394)، صص 1169 -1178مقدمهتاموکسیفن یک داروی غیراستروئیدی است که به طور عمده در درمان سرطان پستان و همچنین برای تحریک تخمک گذاری و درمان نازایی مورد استفاده قرار می گیرد. با توجه به اتصال تاموکسیفن به رسپتورهای استروژن و امکان نقش استروژن ها در اسپرماتوژنز، هدف از برسی حاضر، مطالعه اسپرماتوژنز و بررسی هیستولوژیک مجاری منی ساز در موش های بالغی است که مادران آنها طی دوره حاملگی تاموکسیفن دریافت کرده است.روش بررسیدر این بررسی 30 سر موش ماده و 15سر موش نر از نژاد NMRI، برای عمل جفت گیری انتخاب شدند. از روز 13 حاملگی، به مدت هفت روز، موش های گروه تجربی، داروی تاموکسیفن را با دوز mg/kg 5 به صورت داخل صفاقی دریافت کردند و به موش های گروه کنترل نرمال سالین تجویز گردید. بعد از زایمان موش های مورد مطالعه، نوزادان نر را جدا کرده و تحت مراقبت در شرایط استاندارد قرارگرفتند. پس از رسیدن به سن بلوغ(8-6 هفتگی) موش های بالغ با روش جابجائی گردنی کشته شدند و برای بررسی اسپرماتوژنز، بیضه ها را از شکم خارج کرده و پس از طی مراحل روتین بافتی، با میکروسکوپ نوری مورد مطالعه قرار گرفتند.نتایجمطالعات هیستولوژیک نشان داد که تعداد سلول های اسپرماتوژنیک و سرتولی در لوله های منی ساز در دو گروه آزمایش و کنترل تفاوت معنی داری داشتند ولی تفاوتی در تعداد سلول های لایدیگ در دو گروه مشاهده نشد.نتیجه گیرینتایج این تحقیق نشان داد که، تجویز تاموکسیفن طی دوره تکاملی، می تواند باعث تغییرات هیستولوژیک در لوله های منی ساز گردد و در نتیجه منجر به ناباروری در موش نر گردد.کلید واژگان: تاموکسیفن, ساختار بافتی, بارداری, لوله های منی ساز, اسپرماتوژنزJournal of Shaeed Sdoughi University of Medical Sciences Yazd, Volume:23 Issue: 12, 2016, PP 1169 -1178IntroductionTamoxifen is a nonsteroidal drug which mainly treats breast cancer. It is also applied for stimulation of ovulation and remedy of infertility. Regarding the tamoxifen binding to estrogen receptors and the possible role of estrogens in spermatogenesis, the present study aimed to histologically evaluate spermatogenesis in the seminiferous ducts of mice, whose mothers had received tamoxifen during pregnancy.MethodsIn the present study, 30 female and 15 male mice of NMRI race were selected for mating. Since 13th day of pregnancy, the experimental group received tamoxifen with the dosage of 5 mg/kg intra-peritoneally for 7 days, wherease the control group received normal saline. After childbirth of the mated mice, male infants were selected and monitored in the standard laboratory conditions. After reaching the age of puberty (6-8Weeks), adult mice were sacrificed by the cervical dislocation, and the testes were removed for histological evaluation of spermatogenesis. After routine histological processing, the samples were studied by the light microscope.ResultsHistological studies showed that spermatogenic and Sertoli cells in the seminiferous tubules in control and experimental groups were significantly different, though no difference was observed in the number of Leydig cells in the both groups.ConclusionThe findings of the present study showed that tamoxifen exposure during development can cause histological changes in the seminiferous tubules, which can lead to infertility in the male rat.Keywords: Pregnancy, Seminiferous tubules, Spermatogenesis, Tamoxifen, Tissue structure
-
مقدمهبرنامه ریزی خانواده ها برای استفاده از روش های پیشگیری از بارداری، از مسائل مهم بهداشت جهانی است. در سیستم های پزشکی، خواص دارویی متنوعی ضد دیابت، ضد اکسیدان، ضد بارداری و غیره به نقاط مختلف درخت انبه نسبت داده اند.روش کاردر این پژوهش، 35 سر موش صحرایی نر با وزن تقریبی250 گرم انتخاب و به گروه های کنترل، شاهد و تجربی تقسیم شدند. گروه کنترل آب و غذا به مقدار کافی، گروه شاهد 2/0 میلی لیتر آب مقطر، گروه های تجربی میزان 500، 1000 و1500 (mg/Kg) عصاره برگ انبه روزانه به مدت 28 روز تجویز شد. در روز اول و آخر آزمایش وزن هر موش اندازه گیری شد و جهت سنجش میزان باروری، نمونه ها برای جفت گیری با موشهای ماده همجوار گردیدند. نمونه ها بیهوش و بافت بیضه جهت مطالعات بافت شناسی تثبیت شد. تجویز عصاره برگ انبه موجب کاهش معنی دار وزن بدن و بیضه در سطح P≤ 0.05 شد.یافته هامیانگین وزن بدن و بیضه ها در گروه های تجربی دریافت کننده عصاره نسبت به کنترل کاهش معنی داری نشان دادند. تعداد سلول های جنسی نر، سرتولی و لیدیگ و قطر لوله های منی ساز، ارتفاع اپیتلیوم منی ساز و میزان باروری گروه های تجربی نسبت به کنترل به طور معنی داری کاهش یافت.نتیجه گیریبنابراین احتمالا عصاره آبی برگ انبه روی فعالیت سیستم تولید مثلی نر، اثر کاهشی داشته و می تواند به عنوان ماده ای جهت پیشگیری از بارداری، مفید واقع شود.
کلید واژگان: برگ انبه, اسپرماتوژنز, باروری, لوله های منی ساز, موش صحراییBackgroundTo use appropriate contraceptive methods، families planning have one of the important issues in global health. In medical systems، Medicinal properties have been attributed anti-diabetic، anti-oxidant، anti-fertility، etc. to various parts of mango tree.Materials And MethodsIn this study، 35 male rats approximately 250 g was selected and were divided into: control، testator and experimental groups. The control group received sufficient water and food، 0. 2 ml distilled water received to testator and about 500، 1000 and 1500 mg/ kg extract mango leaf was prescript to experimental group for 28 days. The weight of each mouse was measured and recorded in the first and last days. For evaluation of reproducibility، the samples were nearby with female rats. The samples were anesthetized and fixed for histological studies. Administration of extract mango leaf، significantly was decreased weight of body and testis at level of P≤ 0. 05.ResultsThe results showed that administration of extracted mango leaf، significantly decreased weight of body and testis. The number of male sexual cells، Sertoli and Ledig and the seminiferous tubule diameter، seminiferous epithelium height and fertility rate were decreased significantly.ConclusionExtract mango leaf can be reduced effect on activity of the male reproductive system، and probably، can be recommended as a benefit material for birth control.Keywords: mango leaves, spermatogenesis, fertility, seminiferous tubules, rat -
Background
Spermatogenesis is a highly conserved and regulated process and it is sensitive to fluctuations in the physical and chemical environment. Gemcitabine is a novel antimetabolic anticancer drug used frequently in the treatment of many cancers. This study aimed to investigate the histomorphometric effects of gemcitabine on spermatogenesis in Swiss albino mice.
Materials and MethodsGemcitabine in high and low doses (80 and 40 mg/kg) injected intraperitoneally to inbred Swiss albino mice. Gross testicular features and seminiferous tubular histomorphometry was studies at the end of 7th, 14th day and at 2 months sperm shape abnormalities were studied.
ResultsSeminiferous tubular morphology was altered significantly, showing a reduction in height, perimeter and area in a dose dependent manner. Sertoli cell number decreased. Basement membrane thickness was reduced and it appeared to be permanent, with statistically insignificant changes even after 2 months. There was a reduction of intertubular spaces. Sperms have shown banana heading, decapitation and loss of normal hook of head. The effects were partially reversible at the end of 2 months.
ConclusionIt was concluded that gemcitabine affects the process of spermatogenesis adversely in a dose and time dependent manner and the effects are partially reversible.
Keywords: Gemcitabine, seminiferous tubules, Sertoli cells, spermatogenesis, Swiss albino mice -
BackgroundCadmium chloride which is potentially toxic is currently used in industry. The toxic effects of cadmium on testes have been reported to range from apoptosis to necrosis, with different effects on fertility. This research aimed to study the effect of different doses of cadmium on testicular tissues at acute stage by light and electron microscopy.MethodsCadmium chloride was injected into mature Balb/c mice intraperitoneally in 7 doses. Five mice were studied in each group. After 48 hr, the testes were extracted and prepared for light microscopy. Then two concentrations (15 and 25 µM/kg) of them were selected for electron microscopic study based on histological changes. The cellular changes of luminal epithelium of seminiferous tubules were studied under an electron microscope. Histological and ultrastructural changes were reported.ResultsThe absence of sperm in the tubules was observed at 20 µM/kg concentration. At 25 µM/kg, histological destruction and epithelial damages were observed. Histological changes were not remarkable at 5 and 10 µM/kg. However, ultrastructural changes of seminiferous tubules at 20 µM/kg included spermatogonial cell death. At 25 µM/kg, vacuolation of Sertoli cells and death of spermatids as well as spermatocytes were observed. Cell death in the tubules was limited to germ cells. However, Sertoli cells exhibited architectural alterations without any cell death.ConclusionBoth apoptosis and necrosis occurred in testicular tissue by exposure to cadmium in a concentration-dependent manner. Gonadal cells were sensitive to cadmium administration. Supportive cells such as Sertoli cells in seminiferous tubules did not exhibit sensitivity to cadmium.Keywords: Apoptosis, Cadmium, Necrosis, Seminiferous tubules
-
IntroductionWistar rats are an outbred strain of albino rats belonging to the species Rattus norvegicus. Histopathological studies on the testis was carried out to determine the effects of heat and lead on male fertility by evaluating some andrological parameters of the wistar rat such as morphology of spermatozoa, sperm count and motility. The main objective of this research was to compare the deleterious effect of lead and heat on the male reproductive system of a wistar rat.MethodsFifteen adult male rats were randomly divided into 3 groups: group A were the control group; group B were kept in hot environment above 42 degrees Celsius; and group C were administered 0.18mls of lead acetate solution per grams body weight once daily for 14 days.ResultsThe gonadotoxins caused morphological alterations of sperm cells in this study including head, tail and middle defects which are indications of interference with maturation stage of spermatogenesis in the seminiferous tubules. The deleterious effect of the gonadotoxins on the seminiferous tubules is corroborated by histopathology which revealed degeneration of epithelium of the seminiferous tubules. The degree of sperm cell motility was significantly lowered in animals exposed to gonadotoxins compared to animals in the control group and also the sperm cell count was lowered (P<0.05) in animals exposed to gonadotoxins in comparison with animals in the control group, as a result of decline in the production of normal, viable sperm cells in the test animals.ConclusionIt was concluded that the gonadotoxins exert toxic effect on the seminiferous tubular epithelium with concomitant reduction in the reproductive abilities of the male rats and as such exposure to heat and therefore high level of lead should be avoided both in animal and man.Keywords: Gonadotoxins, Heat, Lead acetate, Spermatogenesis, Seminiferous tubules
-
Objective(s)This study was designed to investigate the effect of Phaleria macrocarpa aqueous extract (PM) on spermatogenesis by observing the histological changes of testes in adult male rats.Materials And MethodsPM was prepared by boiling the dried slices of P. macrocarpa fruits followed by filtering, centrifugation and freeze-drying to obtain the powder form. Eighteen Sprague Dawley adult male rats were divided into three groups (six in each group), designated as treatment (240 mg/kg PM), negative control (distilled water) and positive control (4mg/kg testosterone) and administered via intragastric gavage for seven weeks. In the sixth week of supplementation period, each male rat was introduced to five female rats. Afterward, all rats were sacrificed and the testes were removed for histological studies.ResultsPM significantly increased the number of cell and the thickness of seminiferous tubules of male rats (P<0.05). However, there was no significant effect on the volume and size of testes. The mean of spermatogonia cells numbers of PMgroups differed significantly from the negative and positive groups (P<0.05).ConclusionPM showed potential value as an attractive alternative for improving sexual strength by increasing the number of spermatogonia cell and the thickness of the seminiferous tubules. Perhaps, PM could be suggested to be one of the herbal remedies that can improve men fertility. The results may have some clinical implication in the management of infertility.Keywords: Herbal medicine, Infertility, Phaleria macrocarpa, Seminiferous tubules, Spermatogenesis
-
BackgroundElectromagnetic field (EMF) has harmful effects on different tissues specially testis through different mechanisms such as increase free radicals. Therefore, application of antioxidants may reduce these abnormalities. Polygonum aviculare extract (PAe)as an herbal plant has antioxidant activities. Therefore, in the present study, its protective property against EMF was investigated in testis.Methods6 weeks old male Balb/c mice were divided to control (without EMF exposure), EMF (exposed to 3 mT EMF for 8 weeks), EMF+PAe (received 50 mg/kg PAe and 3mT EMF for 8 weeks) and PAe (received only 50 mg/kg PAe for 8 weeks) groups. After 8 weeks the animals were sacrificed, the testes were dissected out and fixed, and then stained with hematoxylin and eosin (H & E). The sections were subjected to morphometric and histological analysis.ResultsMorphological studies showed that germinal epithelium of testes was negatively affected by EMF and the PAe reduced the harmful effects.ConclusionAs expected, EMF waves have destructive effects on testis morphology via producing free radicals that may cause male infertility. The herbal medicines such as polygonum aviculare extract have antioxidant activity and can reduce the harmful effects of EMF.Keywords: Electromagnetic fields, Seminiferous tubules, Testis, Polygonum avicular
- نتایج بر اساس تاریخ انتشار مرتب شدهاند.
- کلیدواژه مورد نظر شما تنها در فیلد کلیدواژگان مقالات جستجو شدهاست. به منظور حذف نتایج غیر مرتبط، جستجو تنها در مقالات مجلاتی انجام شده که با مجله ماخذ هم موضوع هستند.
- در صورتی که میخواهید جستجو را در همه موضوعات و با شرایط دیگر تکرار کنید به صفحه جستجوی پیشرفته مجلات مراجعه کنید.