جستجوی مقالات مرتبط با کلیدواژه "uncoupling protein 1" در نشریات گروه "پزشکی"

Obesity and overweight are recognized as major risk factors for many diseases. The aim of this study was to determine the effect of eight weeks of resistance training on the expression of apelin and uncoupling protein-1 (UCP1) genes in the visceral adipose tissue of obese male Wistar rats.

In this experimental study, 20 obese male Wistar rats (8 months old, mean weight 350 ± 50.24 g, and height 23 ± 0.9 cm) were randomly divided into two groups: resistance training and control. The training group performed resistance exercises for 8 weeks, 5 sessions per week, each session for about 50-60 minutes, with progressive intensity. The resistance training protocol consisted of climbing a one-meter ladder with 36 steps and an 85-degree slope. Each training session consisted of three sets of five repetitions, with a one-minute rest between each repetition and a two-minute rest between each set. During this period, the control group only stood on the ladder without performing any activity. The expression of apelin and UCP1 genes in visceral adipose tissue was measured using real-time PCR.

The results showed that eight weeks of resistance training significantly decreased the expression of the apelin gene and significantly increased the expression of the UCP1 gene in the visceral adipose tissue of obese rats (P<0.0001).

Resistance training, by reducing the expression of the apelin gene and increasing the expression of the UCP1 gene, likely induces cellular changes that contribute to reducing fat mass and increasing thermogenesis in visceral adipose tissue.

Changes in diet to high-fat and high-calorie diets along with reduced physical activity increase obesity and UCP-1 plays a preventive role against weight gain, obesity, and type 2 diabetes. The present study aimed to evaluation of the effect of aerobic exercise on the expression of UCP1 and MAPK p38 heat gene in subcutaneous adipose tissue in male Wistar rats fed a high-fat diet.

15 rats were randomly divided into 3 groups: normal control, high-fat diet control (40% fat), and high-fat diet+aerobic exercise. After 13 weeks of consumption of a high-fat diet, the aerobic exercise group performed the exercise protocol on a treadmill for 12 weeks and 5 sessions per week. UCP1 and MAPK p38 levels were measured using RT-PCR. One-way ANOVA analysis of variance and Kruskal-Wallis test were used at the level of P≤0.05.

There was no significant difference in UCP-1 mRNA expression between the normal control (P=0.151) and the high-fat diet group but there was a significant difference between the normal control and the high-fat diet+aerobic exercise group (P=0.008). Also, the expression of MAPK p38 in the high-fat diet control had a significant increase compared to the normal control (P=0.008), and a significant decrease in the expression of MAPK P38 in the high-fat diet+aerobic exercise compared to the high-fat diet control was observed (P=0.008).

Aerobic exercise can be a suitable stimulus in the expression of UCP-1. The complexity of MAPK p38 pathway activity is due to its dependence on the type of stimulus and the cell.

Irisin is a myokine that secrets from muscle during and after the exercise and induces non-shivering thermogenesis after stimulation of uncoupling protein-1 (UCP1) gene expression and elevation of number and size of mitochondria. Therefore, irisin activity is effective in reduction of fat percent that lead to treatment of obesity. The aim of this review study was to investigate the effect of various sports activities on irisin levels in healthy adults.

In this systematic review, PubMed and Google Scholar databases were used for articles searching. Keywords include training, exercise, physical activity, irisin and brown adipose tissue. Among 154 articles published between 2010 and 2020, 53 articles were selected. Used parts of selected articles were those related to abstract, biology of irisin, mechanisms of effects of irisin on chronic diseases and mechanisms of effects of exercise on irisin.

Elevation of irisin levels was reported in the most articles (90%) about acute effects of different types of physical activity on Irisin, also irisin levels were consistent after long term exercise in the most related articles (66%).

Results showed that higher exercise intensities were more effective for irisin secretion than lower exercise intensities. Overall, one session exercise increases irisin, while irisin levels remains constant after regular exercise. Also, higher exercise intensity and more muscle bulk usage is more effective for production and secretion of irisin from skeletal muscle that is effective in prevention of diseases such as atherosclerosis, osteoporosis, Alzheimer, cancer and diabetes.

Irisin is a new myokine that has a beneficial effects of exercise on energy metabolism. However, irisin response to different intensities of endurance training is not completely understood. In the current experimental study, 18 male Wistar rats were fed a high-fat diet and 6 rats were nourished a standard diet for 12 weeks. After 12 weeks, obese rats were randomly divided into 3 groups: high-intensity training (34 m/min ~85% VO2max), moderate-intensity training (28 m/min ~70% VO2max) and control. Exercise groups trained 5 days/week for 12 weeks on a rodent treadmill. Serum irisin level and gene expression changes were measured using ELISA and Real Time-PCR method, respectively. In high and moderate-intensity endurance training expression of PGC1-α (P=0.001 and P=0.001), FNDC5 (P=0.013 and P=0.049) and UCP1 (P=0.001 and P=0.001) were significantly more than the control group. Serum irisin level only after high-intensity training was more than the control group (P=0.014). A positive correlation was observed between serum irisin and UCP1 in both of high (P=0.017) and moderate-intensity (P=0.046) training groups. High-intensity endurance training has a positive effect on the level of serum irisin. Also, there is a positive correlation between irisin and UCP1 gene expression in subcutaneous adipose tissue. However, irisin isn’t the only factor affecting on exercise-induced UCP1 gene expression.

The synthesis of uncoupling protein 1 (UCP1) in adipose tissue plays an important role in providing resistance and prevention of fat accumulation, weight gain and obesity. This study was done to evaluate the effect of eight weeks aerobic training with moderate and high intensities on serum levels of Irisin and UCP1 white adipose tissue in obese male rats.
In this experimental study, 24 adult obese male Wistar rats (weight: 250 to 300 gr, BMI>30g/cm2) were randomly assigned into three groups including moderate aerobic training intensity, high intensity aerobic training and control group. The aerobic exercise training was included 8 weeks (5 sessions/per-week for 60 min per session). All training groups carried out aerobic training with 28 m/min (moderate intensity), aerobic training with 34 m/min (high intensity) on treadmill. 48 hours after the training period, the level of UCP1 and Irisin protein was measured.
The level of UCP-1 in adipose tissue and serum Irisin in both aerobic training intensities increased compared to control group, but this increase only in aerobic training group with a moderate intensity was singnificant (p Eight weeks aerobic training with moderate intensity leads to increase of UCP-1 in adipose tissue and Irisin levels.