جستجوی مقالات مرتبط با کلیدواژه « سلول های تک هسته ای خون محیطی » در نشریات گروه « پزشکی »

Numerous studies have shown the anti-inflammatory, antimicrobial, anti-allergic, anti-cancer, anti-spasm, analgesic and wound healing effects of chamomile (Matricaria chamomile). In this study, the effect of aqueous and ethanolic extracts of chamomile on the vital activity of cultured leukocytes of patients with covid-19 was investigated.
After preparation of aqueous and ethanolic extracts of chamomile, peripheral blood mononuclear cells (PBMC) of patients with covid-19 with doses of 200, 300, 400 and 500 micrograms/ml of ethanolic extract of chamomile and doses of 400, 500, 600 and 700 µg/ml chamomile aqueous extract were treated. After 24 hours, the vital activity of PBMCs was measured by MTT method. To analyze the findings, one-way analysis of variance was used in SPSS software version 24. P<0.05 was considered significant.
In the current research, chamomile ethanol extract in doses of 500 micrograms/ml and aqueous extract in doses of 600 and 700 micrograms/ml led to a significant decrease in the vital activity of peripheral blood mononuclear cells compared to the control group.
Aqueous and ethanol extracts of chamomile significantly decrease the vital activity of peripheral blood mononuclear cells; This decrease in vital activity is dose-dependent, and with increasing dose, a decrease in vital activity is observed.

Inflammation is the immune system’s primary response to harmful stimuli. The aim of this study was to evaluate the effect of ethanolic extract of mentha pulegium on the expression of pro- inflammatory mediators of iNOS and NF-κB in mononuclear cells isolated from human peripheral blood activated with lipopolysaccharide.

In this experimental study, mononuclear cells of 12 individuals with no history of specific disease were used. In the first group: PBS buffer was added to the culture media. Group 2: Lipopolysaccharide was used to stimulate inflammation at a concentration of 10 ng/ ml (19). Group 3: After stimulation with lipopolysaccharide, a concentration of 10 μg / ml ethanolic extract of mentha pulegium was used in the culture medium. Group 4: After stimulation with lipopolysaccharide, a concentration of 30 μg / ml ethanolic extract of mentha pulegium was used. Group 5: After stimulation with lipopolysaccharide, a concentration of 60 μg / ml ethanolic extract of mentha pulegium was used. Then, the expression of iNOS and NF-KB pro- inflammatory mediators in different groups wasmeasured. Data analysis was performed using SPSS software with one-way ANOVA analysis and Tukey supplementary test.

The results of the present study showedthat the expression of NF-κB gene in the second group (1.09 ± 0.14) significantly increased compared to the first group (P˂0.05). Moreover, NF-κB gene expression was significantly lower in the third group (0.48 ± 0.09), the fourth group (0.54 ± 0.13), and the fifth group (0.66 ± 0.24) compared to the second group (P˂0.05).In addition,, iNOS gene expression in the second group (1.14 ± 0.22) showed a significant increase compared to the first group (P˂0.05). The expression of iNOS gene in the third group (0.35 ± 0.24) and the fourth group (0.29 ± 0.07) was not significantly different from the second group (P˂0.05).

The results of the present study indicated that the Ethanolic extract of mentha pulegium has  anti- inflammatory effects and can be effective in altering the expression of pro- inflammatory mediators iNOS and     NF-κB in mononuclear cells isolated from human peripheral blood activated with lipopolysaccharide.

Breast cancer is a complex genetic disease. All types of cancer at the cellular level are called genetic disorders, and the implication is that mutations in genes that control cell proliferation can cause cancer. Among the various types of cancer, breast cancer is the most common cancer and the deadliest malignancy among women and is one of the most important health concerns in the world. According to the statistics of the whole country in Iran, breast cancer is the most common cancer in women and every year, about 6160 new cases are found, of which 1063 die from this disease. Breast cancer in man is about 1% reported in women. breast cancer in men appears to be associated with disorders that increase estrogen. Factors influencing breast cancer include gender, age, race and ethnicity, family history, genetic factors, hormonal factors, childbirth, abortion, etc. Symptoms of breast cancer include deformity in the nipple. Discharge from the nipple, breast mass or tumor, changes in the skin of the breast, change in the size of the breast, etc. The increasing number of cancer patients, especially breast cancer in the world and in our country, has raised it as a health problem and has made combating it a health priority. The following common methods can be used to treat breast cancer according to the stage of the disease: Surgery, Radiotherapy, Chemotherapy, Hormone therapies, Gene Therapy, etc. Various factors are involved in the occurrence or even identification of the disease, including noncoding RNAs as diagnostic biomarkers and prognosis of cancer. MicroRNAs are a group of non-coding RNAs that are about 18-25 nucleotides in length and affect gene expression after transcription. Recent studies have shown very important roles in many biological functions of these RNAs, including their role in differentiation, evolution, metabolism, cancer-related processes and even in diseases such as diabetes and Alzheimer's. Cardiac, autism and fragile X Syndrome One of these factors is mir, and mir-96 is one of the MIRs that seems to be important in this disease. The igsf4 gene has also been investigated as a target gene in mir-96. The aim of this study was to investigate the changes in the expression of mir-96 and igsf4 gene in peripheral blood samples of peripheral blood mononuclear cell (PBMCs) of patients with breast cancer.

The present study is a case-control study, 30 blood samples of patients and 30 blood samples of healthy individuals of breast cancer patients were evaluated as controls. Taking into account the mentioned cases and after obtaining written consent and provided that the personal and confidential information of the patients remain confidential, a sample of the mentioned individuals was taken and the subjects entered the testing stage. After sampling, white cells should be isolated from the sample. The next step is to extract RNA, After RNA extraction, the next step is DNA synthesis. Next we have to do PCR for all the sections and genesAnd analysis them. In this study, real time-PCR method was used to study to changes of igsf4 gene expression and The relationship between the results of statistical analysis of data related to NFAT5 and miR-490 gene expression was investigated using SPSS software version 16. Significant level. Finally, statistical analysis of data with P ≤0.05.

the results showed an increase in the expression of mir-96 in patients with breast cancer, on the other hand, no significant changes in igsf4 gene expression were observed in these patients. Another goal of this experiment was the relationship between the expression level of mir-96 and igsf4 gene. From the results of these experiments, it can be concluded that there is a significant relationship between mir-96 and igsf4 gene in mononuclear cells. There is no peripheral blood in PBMC of patients with breast cancer.

According to the observation and results, it can be concluded that in patients with breast cancer, there is an increase in mir-96 and is directly related to this disease, but there was no significant relationship between mir-96 expression and igsf4 gene. This data has been reviewed with SPSS software. The studies are not final studies Due to the limitations of this experiment, it is suggested that in future experiments, this research be re-examined by considering other patients or more diverse patients, using other techniques. and it is suggested that they be reviewed, evaluated and re-studied in other ways as well.

According to the results of some studies, peripheral blood mononuclear cells activated with human placental gonadotropin increase the success rate of pregnancy in infertile women. This study aimed to compare the effect of two treatment methods of peripheral blood mononuclear cells activated with human placental gonadotropin and platelet-rich plasma on the success rate of pregnancy after embryo transfer.

This randomized clinical trial was conducted on 40 females with repeated implantation failure who were candidates for in vitro fertilization (IVF) and referred to Fatemieh Hospital, Hamadan, Iran, in 2019. Subsequently, they were randomly assigned to two groups of A and B. Peripheral blood mononuclear cells activated with human placental gonadotropin were utilized in group A, and group B received platelet-rich plasma autologous blood before transfusion. The incidence of chemical and clinical pregnancies was measured in both groups. Moreover, the data were analyzed in Stata software (version 14), and a p-value less than 0.05 was considered statistically significant.

The mean ages and duration of the infertility of the females were 32.5 and 34.4 years (P=0.203), as well as 9.1 and 8.5 years (P=0.747) in groups A and B, respectively. Moreover, the mean numbers of the obtained ovum, embryos formed, and transferred embryos were 15.4±11.5 and 12.9±8.9 (P=0.439), 7.1±6.2 and 5.8±3.8 (P=0.427), as well as 2.3±0.7 and 2.3±0.6 (P=1.00) in groups A and B, respectively. Furthermore, the rates of chemical pregnancies were 15% and 25% (P=0.619), and clinical pregnancies were 15% and 20% (P=0.581) in groups A and B, respectively.

The administration of human placental gonadotropin-activated peripheral blood cells showed similar results on pregnancy outcome with platelet-rich plasma after an embryo transfer in females who were candidates for IVF with repeated implantation failure.

In previous studies, the effects of food restriction on the changes in immune responses and brain dopamine content have been determined. On the other hand, it has been shown that immune cells, in addition to dopamine production, also have dopamine receptors. The purpose of this study was to evaluate the effect of inhibition of D2 dopamine receptors on several functions of monocytes of peripheral blood in rat under food restriction In this experimental study, 36 male Wistar rats (weighing 200-250 gr) were allocated into six groups (n=6), including control groups, food restriction (25%), food restriction (50%), food restriction (75%), food restriction 75% and Sulpiride and rats treated with Sulpiride. Sulpiride was injected Intracerebroventricular at a concentration of 50 μg / rat on day 21 after the study initiation. At the end, the Rats were bled and peripheral blood mononuclear cells were isolated by ficoll gradient method. Food restriction caused a significant decrease in the activity of monocyte cells of gradient of peripheral blood mononuclear cells like neutral red uptake test and respiratory burst (NBT reduction test) simultaneously with decreasing lymphocytes proliferation after stimulation with phytohemagglutinin. Administration of Sulpiride with a 75% Food restriction resulted in the improvement of these functions of monocyte cells of gradient of peripheral blood mononuclear cells as well as lymphocyte proliferation. Intracerebroventricular administration of dopamine D2 receptor antagonists (sulpiride) effectively inhibited the effects of a severe dietary restriction on the suppression of immunity system.

The pattern of incidence of asthma varies with age and sex, as females suffer more than males. Some asthmatic women report premenstrual exacerbation of asthma symptoms as well as variation of its severity during pregnancy, thus it is believed that sex hormonal changes could affect asthma. Hormone Replacement Therapy (HRT) is a routine and accepted procedure which is used for treatment of several cases such as irregular periods, hirsutism, menopausal manifestations, acne, osteoporosis and amelioration of the symptoms in some autoimmune disease. HRT could reduce the magnitude of variations in estrogen and progesterone over the menstrual cycle. According to increased asthma prevalence among women than men and regarding to expression of estrogen and progesterone receptor on lung and immune cells, we aimed to determine the effects of 17β-estradiol (E2) and progesterone (P4) alone and in combination form on expression of T-bet and IFN-ɣ cytokine secretion, in correlation with Th1 cell subset of Peripheral Blood Mononuclear Cells (PBMC) (as crucial cells that could affect cytokines’ balance) in asthmatic patients versus non-asthmatic healthy controls.
The diagnosis of asthma was confirmed on the basis of clinical symptoms and detection of allergen specific IgE. Then PBMCs were isolated and cultivated in 24-well plates in the presence or absence of 1% phytohemagglutinin (PHA), 10-8 M of estrogen and 10-6 M of progesterone, followed by mRNA isolation. After reverse transcription, real-time quantitative PCR was performed to evaluate the expression level of T-bet. We also measured the concentration of the related cytokine (IFN-ɣ) in supernatants by ELISA.
The expression of T-bet as well as secretion of IFN-ɣ which is a Th1 related cytokine was significantly increased when a combination of both hormones were applied in case group compared to controls [Median: 84.04 (IQR: 77.32-177) and Median: 71.52 (IQR: 68.85-84.04) pg/ml respectively], however, treatment with these hormones alone did not show any significant effects.
We concluded that, treating PBMCs with estrogen and progesterone alone or in combination as an in vitro example of HRT, has stimulatory effect on Th1 cells’ behavior that may have a role in improving (sometimes worsening because of the complex role of CD8 cells) of allergic asthma symptoms. It is crucial to clarify the effect of these hormones on differentiated T helper cell population, which requires more studies to understand the effect of sex hormones on allergic asthma

In the previous studies, the beneficial effects of oral administration of probiotic bacteria in treatment of some gastrointestinal cancer have been documented. The main aim of this study was to evaluate the direct effects of the bacterial cell wall extract Bifidobacterium bifidum on proliferation of human erythromyeloid cancer cell line K562.
Material & After culturing the bacteria, and preparing cell wall fractions, dilutions of 1000, 2000, 4000 and 8000 µg/ml bacteria cell walls were prepared in sterile conditions. K562 cells and peripheral blood mononuclear cells (PBMC) were cultured in a conventional manner in the presence of different concentrations of bacteria from cell wall extract with the doxorubicin concentration of 0.1 mM. In order to assess the anticancer property of the abovementioned extract the MTT test was utilized. The T-Student test was used in order to analyze the data using SPSS 21.
The minimum effective concentration of bacteria extract on the viability of K562 cells and PBMC was 2000 and 4000 µg/ml, respectively.
The selected bacterial extract killed more cancer cells than PBMC cells.

Natural killer T like (NKT) cells are a population of T cells that show NK cells features. These cells in addition to administrative actions, have an important role in the regulation of immune responses. The role of these cells in people exposed to sulfur mustard (SM) are not well known. The aim of this study was to determine the percentage of NKT cells in the peripheral blood of SM exposed people and its association with pulmonary complications.
77 SM exposed patients with lung complications and 62 healthy volunteers as control without any clinical complications were invited to participate. Spirometry was performed and participants were divided into four groups (normal, mild-moderate, severe, and very severe) according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD). Also, percentage of peripheral blood NKT (CD3 CD16/56) in SM exposed individuals and control group was determined with flow cytometry.
There was a significant increase in the percentage of NKT-like cells in exposed group compared with controls. Also, according to GOLD classification, NKT cells percentage showed a significant difference in severe exposed as compared to the control group.
NKT cells have the potential to migrate to the lung. So, these cytotoxic cells identified in the peripheral blood of these patients may be reflective of cell populations in the lung of this patients. However, further studies are necessary in the lung.

Today the use of medicinal plants to improve the immune system function and against pathogenic bacteria has been considered.
In this study, the effect of methanol extract of the aerial parts of Agrimonia eupatoria on peripheral blood mononuclear cells (PBMC) and 12 pathogenic bacteria were investigated.
The methanol extract of branches, stems, seeds and leaves of Agrimonia eupatoria were prepared and the effect of different concentrations of 50, 100, 500, 1000, 1500, 2000 and 2500 μg/ml of the extract on proliferation of PBMC was evaluated by MTT assay. Also the effect of concentrations of 1, 2, 4, 5, 7 and 10 mg/ml of the extract was tested on 12 pathogenic bacteria by disc method and on nutrient agar media.
The methanol extract of the branch, stem and seed of Agrimonia eupatoria showed the most stimulatory effects on immune system and induced the proliferation of PBMCs up to 8 times. Methanol extract of Agrimonia eupatoria showed antibacterial effects against gram-positive bacteria and the most antibacterial effect was on Bacillus subtilis at a concentration of 4 mg/ml and Staphylococcus aureus at concentration of 7 mg/ml.
The Agrimonia eupatoria methanol extract showed stimulatory effects on the immune system and also antibacterial properties against certain gram-positive pathogenic bacteria. These finding indicate that Agrimonia eupatoria can be considered to use for immunodeficiency patients and moreover to control some bacterial infections.

Echinops ilicifolius, Echinops jesdianus, Echinops ceratophorus and Echinops lasiolepis are defined as native plants of Yazd that their immunomedulatory effects have not been studied yet. The aim of this study was to determine the effect of different concentrations of these plants on peripheral blood mononuclear cells(PBMCs) proliferation and interleukin(IL)-4 secretions. Root extracts of Echinops ilicifolius, Echinops jesdianus, Echinops ceratophorus and Echinops lasiolepis were prepared by Maceration method. PBMCs were obtained from three healthy volunteer individuals and cultured with the presence of 0.1,1,10,100 and 200 µg/ml with concentrations of 10 µg/ml of phytohemagglutinin. The rate of cell proliferation was determined by BrdU kit. The IL-4 levels in PBMCs. supernatant were measured by enzyme-linked immunosorbent assay(ELISA). P value<0.05 was considered significant. The different concentrations of root extracts of all plants showed inhibitory effect on PBMCs. There was a significant difference among Echinops lasiolepis extracts in different concentrations(p=0.045). The levels of IL-4 were similar in super natant in control group and different concentrations and the control groups. The results showed that root extracts of Echinops species had inhibitory effect on PBMCs proliferation and in some species with decrease in IL-4 secretion might have immunomedulatory effects. The effect of Echinops extract fractions on PBMC is suggested.

The widespread use of herbal medicines because of their protective effects on various organs has been shown in many studies. Silymarin is a flavonolignan complex isolated from the milk thistle (Silybum marianum L. Gaertn), has been clinically used for its anti-inflammatory and hepatoprotective effects. Recently, immunomodulating effects of this plant have considered by the researchers. This study aimed to investigated the effect of silymarin and DMSO on the human T lymphocyte proliferation under the cell culture conditions. Peripheral blood mononuclear cells (PBMCs) from the healthy individuals were activated with Con A (10 μg/ml) and treated with silymarin at different concentrations (0.001, 0.01, 0.1, 1, 10, 100 and 200 μM). Then, cells were incubated for five days (at 37◦ C with 5% CO2) for proliferation assay using the CFSE dye and for viability analysis using flow cytometry. We analyzed the data using the Graph pad prism (version 6). This study showed that silymarin has the ability to inhibit the T cell proliferation in vitro. Moreover, 100 μM and 200 μM of silymarin (P < 0.001) had more inhibitory effect on T cells. We conclude that Silymarin exerts immunosuppression effects, and it could be used in therapeutic situations with fewer side effects compared with the other immunosuppressive drugs.

Type2 diabetes (T2D) is the most prevalent chronic metabolic disease. MicroRNAs act as regulators of gene expression by inhibiting translation or promoting degradation of target mRNAs. Recent reports indicate that microRNAs also can play important role in establishment of diabetes and their roles in T2D different aspects including insulin insensitivity.To detect a noninvasive screening method for type2 diabetes (T2D) we evaluated the expression level of miR-103 in peripheral blood mononuclear cells (PBMC) samples from pre-diabetes (n = 8) and T2D rats (n = 8) in compare to normal group (n = 8) using real time polymerase chain reaction (RT-PCR).

Animal model T2D was made with combination of high fat diet (HFD) and low dose of Stretozotocin (STZ) injection. Blood glucose level, serum insulin, and cholesterol, low density lipid and triglyceride levels were measured after 10 weeks diet interference. PBMC isolated from blood sample of three group of animals, and then total RNA was obtained using Trizol reagent. All microRNAs converted to cDNA (complementary DNA). Expression level of rno-miR-103 assessed by one step SYBR GreenⅠ relative real time PCR. Using specific LNA TM qRT-PCR primer set for amplifying rno-miR-103. Statistical analysis was performed using SPSS version 20.

Combination of high fat diet and low dose of STZ effectively induced T2D in rats. rno-miR-103 expression level in PBMC samples of pre-diabetic (HFD) subjects and T2D rats significantly elevated in compare to normal group.

The high fat diet/STZ induced T2D animal models is the most suitable for mimicking pre-diabetes and diabetes in men. The result of miRNA expression assay in PBMC indicates the importance of rno-miR-103 in T2D pathogenesis in early stages and PBMC potential as a clinically useful noninvasive source to predict the disease statues.

Background and Royal jelly (RJ) is a substance synthesized by worker bee- is shown to have various biological activities on different cells and tissues of the human body. In this study the effect of RJ on peripheral blood mononuclear cells cytotoxicity against the K562 Erythroleukemia cell line was investigated. In this experimental study human PBMCs (106 cells/ml) were separately cultured in standard conditions. The cells were then incubated with different concentrations of RJ (5 mg/ml, 10 mg/ml, 25 mg/ml) for 72 hours. Subsequently, cytotoxic effect of PBMCs pulsed with RJ on K562 was evaluated by Anexin PI test (Flow cytometry). In the Anexin PI test, apoptotic property and cytotoxic effect of PBMCs pulsed with RJ on K562 increased significantly compared to those of the control group. PBMCs pulsed with RJ increased survival rate of PBMCs after treatment with RJ. This compound could be beneficial in preventing and controlling carcinoma cells.

Tonsils and adenoid hypertrophy is a major respiratory symptom in children which is partly due to recruitment of inflammatory cells in upper airway lymph nodes as a result of the effects of synthesis and release of different inflammatory cytokines. It seems that infections play role in concert with these cytokines leading to tonsilar hypertrophy and other pathologic consequences. It is proposed that cellular infiltrate of tonsils and adenoids may secrete different quantities of these cytokines compared with peripheral blood mononuclear cells (PBMC) cultures. Among patients who were admitted for adenotonsillectomy to the ENT ward، 37 patients، under 1-12 years old patients with fulfill criteria selected to include the study. Excised adenoid and tonsils cultured and inflammatory cytokines Interferon-γ (INF-γ)، Interlukine-1 (IL-1)، IL-6، IL-8 and tumor necrosis factor-α (TNF-α) measured in cellular culture supernatant. The same cytokines measured in PBMC cultures. The data shows that there is a significant difference between IFN-γ and IL-8 amounts in adenoid tissue culture supernatant and PBMC culture of our patients. Furth-ermore، the amounts of IFN-γ، IL-1 and IL-8 showed considerable difference between tonsilar tissue culture supernatant and PBMC culture of these patients. Although there is a significant correlation between IL-6 amounts in tissue culture supernatant and PBMC culture (P=0. 02)، the respective data for TNF is only almost significant. Inflammatory cytokines may have significant role in the early provoke of inflammation occurred in hypertrophied tonsils and adenoid. The majority of these cyt-okines increase the expression of adhesion molecules on epithelial cells and influence the recruitment of leucocytes and inflamed tonsils. On the other hand lack of sufficient cytokine release may lead to persistent infections and may cause chronic inflammation and hypertrophied tissue.