جستجوی مقالات مرتبط با کلیدواژه « copper nanoparticles » در نشریات گروه « پزشکی »

Moringa oleifera is a traditional South Indian medicinal plant and an excellent source of antioxidants.

To evaluate the antimicrobial potential of copper nanoparticles (CuONPs) derived from Moringa oleifera pods against urinary tract-infecting microbes.

Phyto constituents of the Moringa oleifera pod were screened. CuONPs were synthesized from Moringa oleifera pod, and it was characterized using UV-visible spectroscopy, FTIR, and SEM. Antioxidant activity of CuONPs was also determined. CuONPs discs at 50, 75, and 100 µg/ml were prepared and tested for antimicrobial action against Escherichia coli, Enterococcus faecalis, Klebsiella pneumonia, Streptococcus aureus, and Candida albicans. The integrity of the E.coli was assayed by determining the lactate dehydrogenase activity.

GCMS analysis revealed the presence of Di-n-Octyl Phthalate and 2, 2-diethoxy Propane presence in the Moringa oleifera pod. Antioxidant assay revealed the free radical scavenging property of CuONPs. UV- VIS confirmed the synthesis of CuONPs by showing the λ Vmax at 220nm. SEM analysis revealed the CuONPs size in 40 to 160nm. The FTIR analysis exhibited the presence of OH and C = C groups in the CuONPs. The klebsiella pneumoniae was the most susceptible strain for CuONPs, followed by Escherichia coli and Candida albicans. E. coli showed a significant LDH activity at high concentration of CuONPs.

The CuONPs synthesized from the Moringa oleifera pod exhibited significant antimicrobial activity against the microbes.

Vulvovaginal candidiasis (VVC) is a prevalent and often recurrent condition affecting an estimated 75% of women worldwide. Candida albicans is a primary fungal pathogen responsible for a significant proportion of VVC cases. This cross-sectional study investigated the expression levels of two critical virulence genes, ALS1 and HWP1, in C. albicans isolates from women diagnosed with VVC. Moreover, we examined the effect of copper nanoparticles on the expression of these genes, exploring their potential as a novel antifungal therapy for VVC treatment.

This study recruited 30 patients diagnosed with VVC from Razi Hospital, Iran. We employed polymerase chain reaction (PCR) to confirm the presence of the ALS1 and HWP1 genes in C. albicans isolates. Subsequently, we extracted RNA from the isolates and assessed the effect of copper nanoparticles on the expression of ALS1 and HWP1 genes using quantitative real-time PCR (qRT-PCR).

Of the 30 C. albicans clinical isolates analyzed, 17 (56.7%) harbored both HWP1 and ALS1 virulence genes. Copper nanoparticles significantly downregulated the expression of these genes. Notably, treatment with 8.8 μg/mL copper nanoparticles resulted in a significant reduction of HWP1 gene expression, while 3.23 μg/mL copper nanoparticles led to a significant decrease in ALS1 gene expression.

This study identified the presence of ALS1 and HWP1 virulence genes in C. albicans isolates from women with VVC and demonstrated the potential of copper nanoparticles to downregulate their expression. These findings offer promising insights into the development of novel antifungal therapies for VVC treatment. However, further investigations with larger, more diverse cohorts and comprehensive analyses are necessary to fully understand the effects of copper nanoparticles on C. albicans gene expression and their potential clinical applications for VVC management.

In recent years, the green synthesis of nanoparticles has received much attention. Green synthesis has several advantages over other

cost-effectiveness, simplicity, and non-toxicity. In the present study, we obtained the aqueous extract of Thymus daenensis (Celak) flora, biosynthesized the copper nanoparticles (Cu-NPs), and evaluated the antifungal activity.

UV-vis spectroscopy analyses, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) were used to identify the synthesized nanoparticles. The antifungal activity of the synthesized copper nanoparticles was evaluated using the microdilution method.

After adding the extract to the copper sulfate solution, the solution color changed from light blue to yellowish-green. A maximum peak at the wavelength of 414 nm confirmed the copper nanoparticles formation. Scanning electron microscopy demonstrated the particle size ranging from 30 nm to 42 nm. The biosynthesized Cu-NPs had an inhibitory effect against Candida albicans, Fusarium solani, Aspergillus Niger, and Aspergillus flavus.

Our findings demonstrated that T. daenensis aqueous extract acts as a reducer and stabilizer factor. We successfully synthesized Cu-NPs from copper sulfate using T. daenensis (Celak) flora aqueous extract according to the UV-Vis spectrum, FTIR, and SEM results. This research was the first report of Cu-NPs synthesized from an aqueous T. daenensis (Celak) flora extract. Our simple, quick, and inexpensive method for biosynthesis of a nanoparticle, which showed antifungal activity, provides a new potential antifungal agent for therapeutic applications.

Green synthesis of nanoparticles is more simple, inexpensive, nontoxic, and environmentally friendly than traditional methods. Thus, given the presence of secondary metabolites, the use of plant extracts has attracted much attention in causing metal ion reduction reactions into nanoparticles.

In this study, sampling was conducted from pistachio trees of the same age and from branches of the same diameter with a height of 1.5 meters. Leaves and fruits with green and red hull samples were harvested at a specified time. Besides, biochemical properties of the samples were examined for phenolic compounds, anthocyanins, and flavonoids. Copper nanoparticles were synthesized using 1mM copper sulfate salt at 55 and 80 °C. In addition, the absorption spectrum of the synthesized nanoparticles was analyzed through measuring the UV-Vis spectrum.

The content of phenolic compounds in the leaves showed a significant increase compared to the fruit hull. In addition, red hull fruits had less phenols than green hull ones. Besides, anthocyanin and flavonoid contents were higher in the leaves than in the fruit hull. Unlike the phenolic compounds, the anthocyanin content increased upon fruit development. Nanoparticles were synthesized upon observing the change in the color of the plant extract and the copper sulfate from pale yellow to dark brown. In addition, the peak in the wavelength range of 400- 430 nm confirmed the formation of copper nanoparticles.

The results of this study showed that the phenolic compounds, anthocyanins, and flavonoids exiting in the aqueous extract of the pistachio leaves and hull acted as copper-reducing agents and chemical stabilizers of copper metal ions into copper nanoparticles.

Iranian researchers have enriched conventional engine oil with nanoparticles at the Institute of Petroleum Research and hope that this product can replace conventional engine oil due to the improved effects of copper nanoparticles on oils. the purpose of this study was to investigate the pathological effect of engine oil enriched with copper nanoparticles on the rat.

In this study, 72 female rat were randomly divided into 8 experimental groups, and three treatment groups (repeated) oral doses of 2000 mg /kg and 5000 mg /kg engine oil containing and without copper nanoparticles in three treatment groups at 30 minutes, 4 hours and 24 hours.

In treatment group A and B, oral dose of 5000 mg/kg engine oil containing and without copper nanoparticles showed a significant positive correlation with lethality at 30 minutes, 4 hours and 24 hours. p