جستجوی مقالات مرتبط با کلیدواژه "brucellosis" در نشریات گروه "دامپزشکی"

Brucellosis is one of the most common infectious diseases in Iran, which is common between humans and animals and is caused by Brucella species. One of the ways of brucellosis transmission to humans is the consumption of milk and traditional products contaminated with Brucella bacteria. The present study was conducted to investigate the epidemiological situation and the incidence of malt fever in cattle and sheep in Shiraz city. 120 serum samples were taken from cows and 80 serum samples from sheep. First, the rose bengal test was performed on all serum samples as a rapid initial diagnostic test, and it was observed that 5% of the 200 rose bengal test samples were positive. Then the positive samples in the Rose Bengal test were subjected to the Wright tube test and it was observed that 4% of the samples were positive. Then, in order to detect the type of antibody and to determine the active or inactive state of the disease, two mercaptoethanol tests were performed on the said sera, which was 3.5% positive. According to the mentioned results, the prevalence of Brucellosis in cattle and sheep was reported to be 3.5%, and being female (OR=7.4), being old (OR=7.87) and traditional keeping (OR=7.27) increase the risk of brucellosis in cows and sheep. In the present research, contamination was observed in old animals more than in young animals. It was observed that the contamination in the animals that are kept in the traditional way is more than the animals that are in industrial and production units.

Brucellosis is one of the most important diseases common to humans and animals worldwide, both economically and publicly. Cattle brucellosis is usually caused by Brucella abortus and is one of the most important diseases in many countries of the world because of its economic importance. Generally, risk factors for brucellosis can be divided into four groups of management factors (herd size, etc.), animal factors (age, sex, etc.), factors divided by rancher (rancher age, etc.) and geographical area (such as weather conditions, etc.). Therefore, this study investigated the relationship between some risk factors of brucellosis at the animal level. This case-control study was performed on 843 cows including 281 seropositive (case) and 562 seronegative (control). Data were analyzed using Stata 14 software and conditional logistic regression. Being pregnant (OR= 0.44), lactating (OR= 0.43) and having a good vaccination history (OR= 0.09) reduced the risk of infection and being born in another farm (coming from another farm) (OR= 2.04) and having a history of abortion (OR= 3.77) increased the risk of brucellosis infection in dairy cows. Appropriate vaccination and no displacement of livestock is recommended.

Brucellosis caused by Brucella melitensis is one of the important health and economic diseases in Iran is very important to pay attention to it as a zoonotic disease. The aim of the study was to investigate B. melitensis infection in a kennel dog and also to compare different laboratory methods to diagnose Brucella melitensis infection in dogs. In this study, the whole blood samples (for PCR and culture), vaginal swabs samples (for PCR tests) and serum samples (for Rose Bengal, Wright and 2 mercaptoethanol tests) were obtained from 14 bitches at a kennel, in which a bitch had been aborted due to Brucella melitensis infection. Also, the whole blood sample and semen sample were obtained from the only male dog of this kennel for PCR test. Finally, the data obtained from these tests were analyzed by calculating the sensitivity and specificity as well as the Kappa coefficient. Bacteriological culture results of all blood samples were negative. The PCR results of seven blood samples (%50) and eight vaginal swab samples (%57.1) obtained from 14 bitches were positive. By using PCR primers, B. melitensis were identified in all positive samples. In the serological test, out of 14 sera obtained from bitches for the Rose Bengal screening test, eight positive samples were observed, but only one positive sample was observed in Wright and 2 mercaptoethanol tests. The results showed that the sensitivity and specificity of PCR test in vaginal swab samples was 100% and there is an excellent performance (kappa coefficient = 1) between the results of the Rose Bengal test and PCR of vaginal swab samples in the diagnosis of Brucella melitensis in dogs. In dogs suspected of brucellosis, at first it is recommended to take a sample of the animal's blood to perform the Rose Bengal test, and if the result is positive, to confirm the diagnosis and to determine the strain of Brucella, then a vaginal swab sample should be taken to perform the PCR test.

Milk and dairy products are important sources of food-borne pathogens. Non-pasteurized dairy products are popular due to home production, beliefs about their higher nutritional value, high accessibility, and taste.

This study aims to investigate the consumption pattern of local dairy products in women in Qom, Iran, in 2022, and determine the affecting factors.

In this cross-sectional study conducted in 2022, 319 women in Qom were selected using a stratified random sampling method. Their demographic information (age, educational level, employment status, and income) and consumption of local dairy products were surveyed. In addition, a questionnaire based on the theory of planned behavior (TPB) with 32 items and 4 subscales (attitude towards nutrition, subjective norms, behavioral intention, and nutritional behavior) was completed. The data was analyzed in SPSS software using ANOVA, and Chi-square test.

Overall, the consumption rate of local milk was 82.3 %; yogurt, 85.1 %; cheese, 57.3%; cream, 53.7 %; butter, 42.3 %; and curd, 33.9 %. Regarding the daily consumption rate, the highest consumption rate was related to milk (13.9 %) and yogurt (11.8 %), and the lowest consumption was related to curd (3.1%) and cream (5.1 %). The type of dairy consumed was significantly related to behavioral intention and nutritional attitude (P<0.05). There was a significant difference in the type of consumed dairy in terms of the husband's occupation (P=0.001), but there was no significant difference in terms of educational level, marital status, employment status, and relationship with the villagers (P>0.05).

The prevalence of local dairy products consumption, especially milk and yogurt, is high in women living in Qom. Their behavioral intention to consume healthy dairy products is at good level, but they do not have proper nutritional attitude and nutritional behavior. Therefore, the risk of developing common zoonotic diseases, including brucellosis and crimean-Congo hemorrhagic fever is high in Qom.

Brucellosis is one of the five common infectious diseases between humans and animals, caused by contamination with Brucella. Since farmers, students, veterinary medicine staff, and slaughterhouse personnel are considered to be at risk of contamination with this bacterium, it is necessary to estimate the prevalence of the disease in these individuals. This study was conducted on 300 people with high occupational risk (farmers, veterinary staff, veterinary students, slaughterhouse personnel) in Shahrekord City, Iran. Blood serum samples were evaluated using Rose Bengal, Wright, Combs Wright, 2ME, and PCR tests. People's profile was recorded by questionnaire. Data were analyzed using Sigma stat 4 statistical software by McNemart test. The results of the Rose Bengal test showed that out of 300 samples, 46 (15.3%) had a positive reaction, but in the Wright test, 24 (8%) showed a positive reaction with a titer of 1.80 or higher. Wright's test revealed that 12.5% of farmers, 10% of slaughterhouse workers, 5.7% of veterinary staff, and 2% of veterinary students had positive titers against Brucella. In the 2ME test, the rates of positive cases (1:40 and more) among farmers were 8.8%, slaughterhouse workers 7.5%, veterinary staff 5%, and veterinary students 2%. DNA extraction and PCR tests on serum samples showed that 7.5% of farmers, 6.3% of slaughterhouse workers, 5% of veterinary staff, and 2% of veterinary students had the Brucella bacterium genome in their blood serum. The statistical test results showed a significant agreement between the results of the 2ME and PCR tests (P<0.01). According to the results of the present study, the prevalence of brucellosis among farmers, slaughterhouse workers, and butchers was higher than among other groups at risk. There is a close agreement between the results of 2ME and PCR tests; therefore, the PCR can be used as a valid test to diagnose brucellosis.

Brucellosis is considered one of the most important diseases of humans and animals. Due to abortion, reduced milk production, infertility of infected animals, and transfer to humans, this disease is always considered from two economic and health aspects. This study was conducted to investigate the epidemiological characteristics and the incidence of Brucellosis in Kamijan city. This cross-sectional study examined demographic and epidemiological information of 379 people with Brucellosis who had referred to health centers and offices in Komijan between 1390 and 1398. Frequency distribution statistical models, independent t-test, univariate chi-square, and logistic regression were used for analysis using SPSS software version 25. The significance level of the tests was considered 0.05. The disease incidence between 1390 and 1398 was 98 per 100,000 population. The mean age of patients was 38.20±18.97 years, and the highest frequency of reported cases was in the age group of 25-44 years with 38 percent. The infection rate was higher in men (67 percent) than in women (33 percent). Occupationally, the highest disease incidence was in ranchers and farmers, with 37.9 percent. A 70.2 percent of patients lived in rural areas, and only 29.8 percent lived in urban areas; this difference was statistically significant (p<0.001). This study showed that the disease is more common among rancher-farmer men and has a specific seasonal pattern, so special attention to the high-risk group is essential. Due to the many economic and social problems caused by the disease, it is necessary to consider the infection as a health priority, and necessary education about the ways of disease transmission should be given to different population groups, especially the people at risk.

Brucellosis is the most common zoonosis and is a health issue in Iran. Considering this disease is endemic in Qaen, this study intended to investigate the epidemiologic characteristics of brucellosis during 2015-2021. This cross-sectional study was conducted on 421 brucellosis patients, based on the information from Qaen Health Center from 2015 to 2021. To describe the data, frequency, mean, and standard deviation indices and to analyze the data, Chi-square and T-test statistical tests were utilized using SPSS software version 21. The incidence rate was calculated based on 100,000 at-risk populations per year. The average incidence rate of brucellosis from 2015 to 2021 was 135 per 100,000. The highest incidence rate was 156 per 100,000 people in 2021, and the lowest was 20.58 per 100,000 in 2017. The mean age of brucellosis patients was 36.78 ± 19.85  years. The most frequent clinical symptoms were musculoskeletal pain in 317 cases (75.3%) and fever in 160 cases (38%). The consumption history of unpasteurized dairy products such as milk and cheese was 33% and 27%, respectively. Farmers and ranchers, with 239 cases (56.7%), and the rural living population, with 282 cases (67%), were the most frequent cases of brucellosis. The incidence rate of brucellosis in Qaen has an upward trend and is higher than the provincial and national averages. According to the latest national guidelines, this county is classified as a highly polluted region.

Brucellosis is a common disease between humans and animals with global distribution and an increasing trend. The control of brucellosis in humans depends on the control of the animal disease. Although there is no approved vaccine for brucellosis in humans, many efforts have been made to develop vaccines with high efficiency and low side effects for animals. Live attenuated vaccines are cheaper and more effective than other vaccines, but they can increase antibiotic resistance, interfere with serological diagnostic tests, and increase virulence in animals. Vaccination of animals with commercially available vaccines may cause disease and, in some cases, have low efficacy.  Vaccines containing recombinant protein or their coding DNA can be suitable alternatives to the conventional vaccines, because they can reduce unwanted side effects. Choosing the suitable antigen that stimulates the immune system is very important in the preparation of these vaccines. In the present study, by observing the new recombinant vaccines that are made based on recombinant proteins and DNA, it has been tried to clarify their strengths and weaknesses and the new perspective of vaccination against brucellosis. There is currently no approved vaccine for brucellosis in humans. In addition, the control of brucellosis in humans depends on the control of the animal disease. This review article searches Google scholar, PubMed, and SID databases for new research on recombinant protein and DNA vaccines against brucellosis until 2021. Considering the advantages and disadvantages of common and new brucellosis vaccines, recombinant and DNA vaccines, after conducting the research stages in large animals, can be a suitable approach to prevent the progress of brucellosis.

Prevention, control and eradication of brucellosis in areas where the disease is endemic requires sufficient epidemiological information to apply policies. The present study was conducted with the aim of epidemiological investigation of brucellosis among the ruminant population of Lorestan province. In this cross-sectional descriptive study, all studies related to brucellosis in livestock were analyzed in Lorestan province. The results obtained from this study show that brucellosis has a high prevalence (22%) in three ruminant species (including cows, sheep and goats) of Lorestan province. The highest prevalence of brucellosis in sheep, goat, and cow milk was determined to be 25.6%, 23.5%, and 21.3%, respectively. Brucella abortus is the predominant species in milk obtained from ruminants in Lorestan province. Rev. 1 vaccine strain is excreted to a high extent (7.5%) in sheep and goats' milk. In general, unpasteurized milk is introduced as a threat to public health in Lorestan province. More extensive studies should be conducted, and existing methods of controlling and eradicating brucellosis should be modified.

Brucellosis or Malta fever is a public health concern and an economically important disease with a worldwide distribution. After rabies, it is important zoonotic disease. The disease is endemic in some geographical areas, including Iran and it is one of the important problems in livestock and humans. The control of disease is highly dependent on the seroprevalence of this disease in livestock. Therefore, the aim of the present study is to evaluate the seroprevalence of brucellosis in sheep in Tabriz to better control this disease in this region. A total of 200 serum samples were obtained from sheep and brucellosis were detected using the Rose Bengal test. The overall prevalence of brucellosis was 18.5% in sheep. The prevalence of brucellosis was 2% in male and 16.5% in female. The odds of brucellosis exposure in > 1 Year sheep (adult) were about 2 times higher than that of <1 Year sheep. The results of the present study showed that the prevalence of ovine brucellosis in the Tabriz Zone was relatively high. Age groups and sex were associated risk factors with the seroprevalence of this disease. Thus, a control and prevention programs of this disease are very important in this region.

Spondylitis is actually vertebral osteomyelitis, and if the infection also involves discs, it is called discospondylitis, which is a complication of diseases such as tuberculosis and Brucellosis.

This study aimed to determine radiological properties of discospondylitis due to tuberculosis and brucellosis infections differentially in German Shepherd dogs.

This retrospective cross-sectional study was carried out in affected German Shepherd dogs with chronic discospondylitis due to tuberculosis (TB) and Brucellosis. Data were collected based on clinical and laboratory symptoms, as well as plain radiography and myelography findings. : In total, 13 dogs were studied, of which 9, including 5 males (55.6%) and 4 females (44.4%), were affected with Brucellosis, and 4, including 2 males (50%) and 2 females (50%), were infected with TB. In the diagnostic imaging of the vertebrae, degenerative lesions in affected dogs with TB were significantly more than those affected with brucellosis (with a significant level P=0.026<0.05). Sclerotic lesions were observed in 44.4% of the brucellosis patients and 25% of the TB patients, while lytic lesions were found in less than 50% of brucellosis patients and more than 70% of TB patients.

Based on this study, degenerative and lytic lesions in TB cases were more than brucellosis patients; however, sclerotic lesions in TB patients were less than brucellosis patients. The severity of each of these complications and the site of involvement of the vertebrae can be differential features for diagnosing the radiology of TB from Brucellosis in this breed of dogs.

Brucellosis or Malta fever is one of the most prevalent zoonotic diseases considered as a health and economic concern.

The current study aimed to employ several methods to detect Brucella in blood and milk samples of saanen goat and use a safe and definitive method to diagnose this disease.

In this study, 122 blood samples and 122 milk samples were collected from saanen goats. After culture and serological-based isolation methods (RBPT, Wright, 2ME, and Ring test), DNA was extracted from all the blood and milk samples. PCR was carried out using B4 and B5 primers on all the extracted DNAs in order to detect the B. abortus and B. melitensis; PCR was carried out with Br.a and Br.m primers.

The results of all the blood samples were negative, but bacterial growth was observed in three milk samples, which was detected in biotyping, biovar 1 melitenensis. The PCR results for detection of Brucella spp. of nine blood samples and nine milk samples were positive. Using mPCR primers, B. melitensis were identified through all the nine milk and blood samples.

Herein, we found that better bacterial diagnostic system and choosing an appropriate technique for rapid detection, such as PCR and Real Time PCR, in addition to popular awareness and other functions of national veterinary medicine institute could control the diseases and decrease their incidence successfully.