جستجوی مقالات مرتبط با کلیدواژه « razi institute » در نشریات گروه « دامپزشکی »

The most effective way to prevent fowl pox disease is to vaccinate and the aim of this study was to determine the immunity level of fowl pox vaccine and also to determine the efficacy of the vaccine against the pathogenic agent, which seems necessary and important to do so. The experiments of this study are summarized in two sections. The first stage includes the vaccination of the experienced chicks in the farm and the evaluation of the effect of the vaccine on the farm, and the second phase included a challenge test carried out in the vaccinated pullets. In order to realize this, fowl pox vaccine of the Razi Institute, which is live attenuated, was inoculated into a curtain under the wing in a layer flock with sixty thousands of Bowans strain laying hen farm was used for testing. Seven to ten days after vaccination, the immune response of the herd was investigated in 10% of the layer flock was taken and the percentage was 96.7%. Then, in order to the challenge test, 30 of these pullets were divided into two separate halls, the first group consisting of vaccinated pullets and the second group that did not inoculate the vaccine. The pathogenic virus was inoculated to them and the control group showed all the symptoms of the disease, and all the birds in the control group had no symptoms. From the above experiments, it is concluded that fowl pox vaccine of the Razi Institute has suitable efficacy.

Leptospira interrogans is the cause of leptospirosis, a common disease among humans and animals. One of the most important recommendations of international associations concerned with laboratory animals, is the use of defined animals. Based on the recommendation of the FELASA, the diagnostic monitoring is required to evaluate the contamination of animals to different microbial agents. In this study, the prevalence of this bacteria was investigated in a colony of NIH strain laboratory mice of laboratory animal breeding unit at the Razi Institute in 1396.74 adult mice (in the range of 30-35g and age 6-8 weeks) were randomly selected from both sexes in breeding colony and 6 wild mice were selected by means of live traps from the outside of facility. After ethical euthanasia and autopsy, samples were taken from their kidney tissues and after preparation, the PCR method was performed using Leptospira interrogans specific primers. Designing and preparing the positive control was carried out using plasmid (pGEM -T easy vector) transformation containing the pathogenic of Leptospira interrogans gene (LipL32) in E.coli for gene amplification and extraction. No positive cases were observed from the examined samples. According to FELASA instructions, it can be concluded that with 99.9% confidence, the Leptospira interrogans infection in breeding salons of Razi Institute NIH mice was discarded.

Infectious laryngotracheitis (ILT) is an acute viral respiratory tract of chickens with worldwide outbreak, and its virus latency, presence of carrier chickens, and long-term virus separation have been increased the difficulty of ILT control. Because of considerable economic losses associated with ILT, vaccination of susceptible pullets with attenuated viruses is recommended in endemic areas. The aim of the present study was to compare the immunization of ILT vaccine produced by Razi Vaccine and Serum Research Institute in White Bovans and HyLine W36 pullets. Seventy thousand pullets of each laying line were allotted into two different farms and were vaccinated with Razi ILT vaccine through eye drop method at eight week of age. In order to determine sera antibody titers against ILT virus by ELISA and serum virus neutralization tests, blood samples were randomly collected from 20 chickens per group before (d=0), and at 3, 6, 8, 10, 12, and 16 weeks post-vaccination. In addition, three weeks following vaccination, 20 birds per group were challenged with a nasal drop of virulent infectious laryngotracheitis (vILT) virus strain to evaluate vaccine effecacy. Results showed that Razi ILT vaccine caused a similar performance regarding serological ELISA antibody titers against ILT and the virus neutralization index in the both strains (P > 0.05). Trends in ELISA antibody tiers against ILT and the virus neutralization index revealed that these incidies reached to the protective immunity threshold three weeks post-vaccination. Moreover, Razi ILT vaccine was effectively protected 100% and 95% of White Bovans and HyLine W36 pullets against a vILT challenge, respectively. In conclusion, vaccination of White Bovans and HyLine W36 pullets with Razi ILT vaccine produced sufficient sera titers against ILT, resulted in effective immune protection against vILT virus. However, there was no difference between White Bovans and HyLine W36 pullets regarding the evaluated immune insides following vaccination.

Razi vaccine and serum research Institute is known as the oldest and biggest manufacturer of the livestock vaccines. This Institute has an important role in general hygiene and public health. In the ground of customer satisfaction, offering of high quality products and establishment of ISO system in Razi Institute, we accomplished farmer´s satisfaction and their attitude about RAZI Brucellosis , Anthrax and FMD Vaccines in five provinces : Tehran, Alborz, East Azarbaijan , Esfehan and Khorasan Razavi. Data collection was done with researcher – made questionnaire .Content validity was confirmed by specialists and internal reliability of the questionnaire was determined by Alfa coronbagh coefficient in a pilot study. Satisfaction and attitude were evaluated in two forms of quantitative and ordinal qualitative variables. Satisfaction was evaluated ineffectiveness, safety and acceptability items. Farmers were selected in the form of available sampling from industrial cattle with more than 100 cows. 428 farmers were enrolled. Farmers´ mean satisfaction level was 80.6± 11.2 and satisfaction level about availability of the vaccine was higher than the other area, so that 86% of participating ranchers had desirable satisfaction level of access fields. 94% of participants had total positive attitude regarding to the livestock vaccination. Attitude and Satisfaction level of farmers about safety of these studied livestock vaccines was less than the other fields.